SSGCID: Target Page

ButhA.00010.h

Oxidoreductase, short-chain dehydrogenase/reductase family

CENTER ID:	ButhA.00010.h
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00010.h.B1.GE36009	Full length( ButhA.00010.h )		1	269

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.3934
RefSeq:	YP_441725.1
UniProt:	Q2SZC4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MADRPKGSGA VNRLAGKVAL VTGAGRGIGA AIARAFAREG AAVAIAELDA ALADETVDAI ARDVADARVL AVPADVAQAE SVAAALACTE RAFGPLDVLV NNAGVNVFGD PLALAEEDWR RCFAIDLDGV WHGCRAALPG MVERGRGSIV NIASTHAFKI IPGCFPYPVA KHGVLGLTRA LGVEYAPRNV RVNAIAPGYI ETQSTHDWWN AQPDPEAARR ETLALQPMKR IGRADEVAMT AVFLASDEAP FINASCITID GGRSVLYHD

NT Sequence

ATGGCGGATC GCCCGAAAGG GAGCGGCGCG GTGAACCGGC TCGCGGGCAA GGTCGCCCTC GTGACGGGCG CGGGACGCGG CATCGGCGCG GCGATCGCGC GTGCGTTCGC GCGCGAAGGC GCGGCCGTCG CGATTGCGGA GCTCGACGCG GCGCTCGCCG ACGAAACCGT CGACGCGATC GCGCGCGACG TGGCCGATGC GCGCGTGCTC GCGGTGCCAG CGGACGTCGC GCAAGCCGAG TCGGTCGCGG CGGCGCTCGC GTGCACGGAG CGCGCGTTCG GCCCGCTCGA CGTGCTCGTC AACAACGCAG GCGTCAACGT GTTCGGCGAT CCGCTCGCGC TTGCCGAAGA AGACTGGCGG CGCTGCTTCG CGATCGATCT CGACGGCGTC TGGCACGGCT GCCGCGCGGC GCTGCCGGGC ATGGTCGAGC GCGGTCGGGG CAGCATCGTG AACATCGCGT CGACGCACGC GTTCAAGATC ATCCCGGGCT GCTTTCCGTA CCCGGTCGCG AAGCACGGCG TGCTGGGCCT CACGCGCGCG CTCGGCGTCG AATATGCGCC GCGCAACGTG CGCGTGAACG CGATCGCGCC CGGCTACATC GAGACGCAAT CGACACATGA CTGGTGGAAC GCGCAGCCCG ACCCCGAGGC CGCGCGCCGC GAAACGCTCG CACTGCAGCC GATGAAGCGG ATCGGGCGTG CGGACGAAGT CGCGATGACC GCGGTGTTTC TCGCATCGGA CGAGGCGCCG TTCATCAACG CGAGCTGCAT CACGATCGAC GGCGGCCGAT CGGTGCTGTA CCACGAC

Details for ButhA.00010.h.B1.GE36009

HARVESTED ON:	10/2/2012
SEQUENCED ON:	10/2/2012
EXPECTED MW:	29kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA DRPKGSGAVN RLAGKVALVT GAGRGIGAAI ARAFAREGAA VAIAELDAAL ADETVDAIAR DVADARVLAV PADVAQAESV AAALACTERA FGPLDVLVNN AGVNVFGDPL ALAEEDWRRC FAIDLDGVWH GCRAALPGMV ERGRGSIVNI ASTHAFKIIP GCFPYPVAKH GVLGLTRALG VEYAPRNVRV NAIAPGYIET QSTHDWWNAQ PDPEAARRET LALQPMKRIG RADEVAMTAV FLASDEAPFI NASCITIDGG RSVLYHD

Validated NT Sequence

atggctcacc accaccacca ccatatggcg gatcgcccga aagggagcgg cgcggtgaac cggctcgcgg gcaaggtcgc cctcgtgacg ggcgcgggac gcggcatcgg cgcggcgatc gcgcgtgcgt tcgcgcgcga aggcgcggcc gtcgcgattg cggagctcga cgcggcgctc gccgacgaaa ccgtcgacgc gatcgcgcgc gacgtggccg atgcgcgcgt gctcgcggtg ccagcggacg tcgcgcaagc cgagtcggtc gcggcggcgc tcgcgtgcac ggagcgcgcg ttcggcccgc tcgacgtgct cgtcaacaac gcaggcgtca acgtgttcgg cgatccgctc gcgcttgccg aagaagactg gcggcgctgc ttcgcgatcg atctcgacgg cgtctggcac ggctgccgcg cggcgctgcc gggcatggtc gagcgcggtc ggggcagcat cgtgaacatc gcgtcgacgc acgcgttcaa gatcatcccg ggctgctttc cgtacccggt cgcgaagcac ggcgtgctgg gcctcacgcg cgcgctcggc gtcgaatatg cgccgcgcaa cgtgcgcgtg aacgcgatcg cgcccggcta catcgagacg caatcgacac atgactggtg gaacgcgcag cccgaccccg aggccgcgcg ccgcgaaacg ctcgcactgc agccgatgaa gcggatcggg cgtgcggacg aagtcgcgat gaccgcggtg tttctcgcat cggacgaggc gccgttcatc aacgcgagct gcatcacgat cgacggcggc cgatcggtgc tgtaccacga ctaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgg atcgcccgaa agggagcggc gcggtgaacc ggctcgcggg caaggtcgcc ctcgtgacgg gcgcgggacg cggcatcggc gcggcgatcg cgcgtgcgtt cgcgcgcgaa ggcgcggccg tcgcgattgc ggagctcgac gcggcgctcg ccgacgaaac cgtcgacgcg atcgcgcgcg acgtggccga tgcgcgcgtg ctcgcggtgc cagcggacgt cgcgcaagcc gagtcggtcg cggcggcgct cgcgtgcacg gagcgcgcgt tcggcccgct cgacgtgctc gtcaacaacg caggcgtcaa cgtgttcggc gatccgctcg cgcttgccga agaagactgg cggcgctgct tcgcgatcga tctcgacggc gtctggcacg gctgccgcgc ggcgctgccg ggcatggtcg agcgcggtcg gggcagcatc gtgaacatcg cgtcgacgca cgcgttcaag atcatcccgg gctgctttcc gtacccggtc gcgaagcacg gcgtgctggg cctcacgcgc gcgctcggcg tcgaatatgc gccgcgcaac gtgcgcgtga acgcgatcgc gcccggctac atcgagacgc aatcgacaca tgactggtgg aacgcgcagc ccgaccccga ggccgcgcgc cgcgaaacgc tcgcactgca gccgatgaag cggatcgggc gtgcggacga agtcgcgatg accgcggtgt ttctcgcatc ggacgaggcg ccgttcatca acgcgagctg catcacgatc gacggcggcc gatcggtgct gtaccacgac tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat