ButhA.00010.g

Oxidoreductase, short chain dehydrogenase/reductase family

CENTER ID: ButhA.00010.g
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: in PDB
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
ButhA.00010.g.B1.GE36008 Full length( ButhA.00010.g ) 1 271
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
ButhA.00010.g.B1.PS01774 Full length( ButhA.00010.g ) 1 271
Structures
4LVU
DEPOSITED: 7/26/2013
DETERMINATION: XRay
CLONE: ButhA.00010.g.B1.GE36008
PROTEIN: ButhA.00010.g.B1.PS01774
5WJS
DEPOSITED: 7/24/2017
DETERMINATION: XRay
CLONE: ButhA.00010.g.B1.GE36008
PROTEIN: ButhA.00010.g.B1.PS01774
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_164060
PATRIC ID: fig|271848.6.peg.3938
RefSeq: YP_441729.1
UniProt: Q2SZC0
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDIASHRES NGARSAQDER YARYPSLAGR AVLITGGATG IGASFVEHFA RQGARVAFVD LDEQAARALA ARLADAAHEP VFVACDLTDI AALRGAIEAI RARIGPIAAL VNNAANDVRH AIADVTPDSF DACIAVNLRH QFFAAQAVID DMKRLGGGSI VNLGSISWML KNAGYPVYAS AKAAVQGLTR ALARELGPFG IRVNTLVPGW VMTDKQRRLW LDDAGRAAIK AGQCIDAELL PGDLARMALF LAADDSRMIT AQDVVVDGGW A
NT Sequence
ATGAGCGACA TTGCCTCGCA TCGCGAATCG AACGGCGCGC GCAGCGCGCA AGACGAGCGT TACGCACGCT ACCCGAGCCT CGCCGGGCGC GCGGTGCTGA TCACGGGCGG CGCGACGGGC ATCGGCGCGT CGTTCGTCGA ACACTTCGCG CGGCAGGGCG CGCGCGTCGC GTTCGTCGAT CTCGACGAAC AAGCCGCGCG CGCGCTCGCC GCGCGGCTTG CCGACGCCGC GCACGAACCG GTGTTCGTCG CGTGCGACCT GACCGACATC GCCGCGCTGC GCGGCGCGAT CGAAGCGATC CGCGCGCGCA TCGGCCCGAT CGCGGCGCTC GTGAACAACG CGGCGAACGA CGTGCGCCAC GCGATCGCCG ACGTGACGCC CGATTCGTTC GACGCGTGCA TCGCGGTGAA CCTGCGCCAT CAGTTCTTCG CCGCGCAGGC GGTGATCGAC GACATGAAGC GGCTGGGCGG CGGCTCGATC GTCAATCTCG GCTCGATCAG CTGGATGCTG AAGAACGCCG GCTATCCGGT CTACGCGAGC GCGAAGGCGG CGGTGCAGGG GCTCACGCGC GCGCTCGCGC GCGAGCTCGG GCCGTTCGGC ATTCGCGTGA ATACGCTCGT GCCCGGCTGG GTGATGACCG ACAAGCAGCG TCGCTTGTGG CTCGACGACG CGGGCCGCGC GGCGATCAAG GCGGGGCAGT GCATCGACGC GGAGCTGCTT CCGGGCGACC TCGCGCGGAT GGCGCTCTTT CTCGCGGCGG ACGACAGCCG GATGATCACC GCGCAGGACG TTGTCGTCGA CGGCGGCTGG GCA
Details for ButhA.00010.g.B1.GE36008
HARVESTED ON: 10/2/2012
SEQUENCED ON: 10/1/2012
EXPECTED MW: 29kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 96
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS DIASHRESNG ARSAQDERYA RYPSLAGRAV LITGGATGIG ASFVEHFARQ GARVAFVDLD EQAARALAAR LADAAHEPVF VACDLTDIAA LRGAIEAIRA RIGPIAALVN NAANDVRHAI ADVTPDSFDA CIAVNLRHQF FAAQAVIDDM KRLGGGSIVN LGSISWMLKN AGYPVYASAK AAVQGLTRAL ARELGPFGIR VNTLVPGWVM TDKQRRLWLD XAGRAAIKAG QCIDAELLXG DLARMALFLA ADDSRMITAX XLSSXAAGXK
Validated NT Sequence
ttgnnnnnnt taagaaggag anataccatg gctcaccacc accaccacca tatgagcgac attgcctcgc atcgcgaatc gaacggcgcg cgcagcgcgc aagacgagcg ttacgcacgc tacccgagcc tcgccgggcg cgcggtgctg atcacgggcg gcgcgacggg catcggcgcg tcgttcgtcg aacacttcgc gcggcagggc gcgcgcgtcg cgttcgtcga tctcgacgaa caagccgcgc gcgcgctcgc cgcgcggctt gccgacgccg cgcacgaacc ggtgttcgtc gcgtgcgacc tgaccgacat cgccgcgctg cgcggcgcga tcgaagcgat ccgcgcgcgc atcggcccga tcgcggcgct cgtgaacaac gcggcgaacg acgtgcgcca cgcgatcgcc gacgtgacgc ccgattcgtt cgacgcgtgc atcgcggtga acctgcgcca tcagttcttc gccgcgcagg cggtgatcga cgacatgaag cggctgggcg gcggctcgat cgtcaatctc ggctcgatca gctggatgct gaagaacgcc ggctatccgg tctacgcgag cgcgaaggcg gcggtgcagg ggctcacgcg cgcgctcgcg cgcgagctcg ggccgttcgg cattcgcgtg aatacgctcg tgcccggctg ggtgatgacc gacaagcagc gtcgcttgtg gctcgacnac gcgggccgcg cggcgatcaa ggcggggcag tgcatcgacg cggagctgct tcngggcgac ctcgcgcgga tggcgctctt tctcgcggcg gacgacagcc ggatgatcac cgcgcnnncg ttgtcgtcga nngcngctgg gnataagtga gtaanatnng atnnnnnntg nnnannannc ccnaang
Expected Protein Sequence
MAHHHHHHMS DIASHRESNG ARSAQDERYA RYPSLAGRAV LITGGATGIG ASFVEHFARQ GARVAFVDLD EQAARALAAR LADAAHEPVF VACDLTDIAA LRGAIEAIRA RIGPIAALVN NAANDVRHAI ADVTPDSFDA CIAVNLRHQF FAAQAVIDDM KRLGGGSIVN LGSISWMLKN AGYPVYASAK AAVQGLTRAL ARELGPFGIR VNTLVPGWVM TDKQRRLWLD DAGRAAIKAG QCIDAELLPG DLARMALFLA ADDSRMITAQ DVVVDGGWA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg acattgcctc gcatcgcgaa tcgaacggcg cgcgcagcgc gcaagacgag cgttacgcac gctacccgag cctcgccggg cgcgcggtgc tgatcacggg cggcgcgacg ggcatcggcg cgtcgttcgt cgaacacttc gcgcggcagg gcgcgcgcgt cgcgttcgtc gatctcgacg aacaagccgc gcgcgcgctc gccgcgcggc ttgccgacgc cgcgcacgaa ccggtgttcg tcgcgtgcga cctgaccgac atcgccgcgc tgcgcggcgc gatcgaagcg atccgcgcgc gcatcggccc gatcgcggcg ctcgtgaaca acgcggcgaa cgacgtgcgc cacgcgatcg ccgacgtgac gcccgattcg ttcgacgcgt gcatcgcggt gaacctgcgc catcagttct tcgccgcgca ggcggtgatc gacgacatga agcggctggg cggcggctcg atcgtcaatc tcggctcgat cagctggatg ctgaagaacg ccggctatcc ggtctacgcg agcgcgaagg cggcggtgca ggggctcacg cgcgcgctcg cgcgcgagct cgggccgttc ggcattcgcg tgaatacgct cgtgcccggc tgggtgatga ccgacaagca gcgtcgcttg tggctcgacg acgcgggccg cgcggcgatc aaggcggggc agtgcatcga cgcggagctg cttccgggcg acctcgcgcg gatggcgctc tttctcgcgg cggacgacag ccggatgatc accgcgcagg acgttgtcgt cgacggcggc tgggcatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for ButhA.00010.g.B1.PS01774
PURIFICATION DATe: 3/12/2013
CONCENTRATION: 48.9mg/ml
OBSERVED MW: 29kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 96
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS DIASHRESNG ARSAQDERYA RYPSLAGRAV LITGGATGIG ASFVEHFARQ GARVAFVDLD EQAARALAAR LADAAHEPVF VACDLTDIAA LRGAIEAIRA RIGPIAALVN NAANDVRHAI ADVTPDSFDA CIAVNLRHQF FAAQAVIDDM KRLGGGSIVN LGSISWMLKN AGYPVYASAK AAVQGLTRAL ARELGPFGIR VNTLVPGWVM TDKQRRLWLD XAGRAAIKAG QCIDAELLXG DLARMALFLA ADDSRMITAX XLSSXAAGXK
Validated NT Sequence
ttgnnnnnnt taagaaggag anataccatg gctcaccacc accaccacca tatgagcgac attgcctcgc atcgcgaatc gaacggcgcg cgcagcgcgc aagacgagcg ttacgcacgc tacccgagcc tcgccgggcg cgcggtgctg atcacgggcg gcgcgacggg catcggcgcg tcgttcgtcg aacacttcgc gcggcagggc gcgcgcgtcg cgttcgtcga tctcgacgaa caagccgcgc gcgcgctcgc cgcgcggctt gccgacgccg cgcacgaacc ggtgttcgtc gcgtgcgacc tgaccgacat cgccgcgctg cgcggcgcga tcgaagcgat ccgcgcgcgc atcggcccga tcgcggcgct cgtgaacaac gcggcgaacg acgtgcgcca cgcgatcgcc gacgtgacgc ccgattcgtt cgacgcgtgc atcgcggtga acctgcgcca tcagttcttc gccgcgcagg cggtgatcga cgacatgaag cggctgggcg gcggctcgat cgtcaatctc ggctcgatca gctggatgct gaagaacgcc ggctatccgg tctacgcgag cgcgaaggcg gcggtgcagg ggctcacgcg cgcgctcgcg cgcgagctcg ggccgttcgg cattcgcgtg aatacgctcg tgcccggctg ggtgatgacc gacaagcagc gtcgcttgtg gctcgacnac gcgggccgcg cggcgatcaa ggcggggcag tgcatcgacg cggagctgct tcngggcgac ctcgcgcgga tggcgctctt tctcgcggcg gacgacagcc ggatgatcac cgcgcnnncg ttgtcgtcga nngcngctgg gnataagtga gtaanatnng atnnnnnntg nnnannannc ccnaang
Expressed Protein Sequence
MAHHHHHHMS DIASHRESNG ARSAQDERYA RYPSLAGRAV LITGGATGIG ASFVEHFARQ GARVAFVDLD EQAARALAAR LADAAHEPVF VACDLTDIAA LRGAIEAIRA RIGPIAALVN NAANDVRHAI ADVTPDSFDA CIAVNLRHQF FAAQAVIDDM KRLGGGSIVN LGSISWMLKN AGYPVYASAK AAVQGLTRAL ARELGPFGIR VNTLVPGWVM TDKQRRLWLD DAGRAAIKAG QCIDAELLPG DLARMALFLA ADDSRMITAQ DVVVDGGWA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCG ACATTGCCTC GCATCGCGAA TCGAACGGCG CGCGCAGCGC GCAAGACGAG CGTTACGCAC GCTACCCGAG CCTCGCCGGG CGCGCGGTGC TGATCACGGG CGGCGCGACG GGCATCGGCG CGTCGTTCGT CGAACACTTC GCGCGGCAGG GCGCGCGCGT CGCGTTCGTC GATCTCGACG AACAAGCCGC GCGCGCGCTC GCCGCGCGGC TTGCCGACGC CGCGCACGAA CCGGTGTTCG TCGCGTGCGA CCTGACCGAC ATCGCCGCGC TGCGCGGCGC GATCGAAGCG ATCCGCGCGC GCATCGGCCC GATCGCGGCG CTCGTGAACA ACGCGGCGAA CGACGTGCGC CACGCGATCG CCGACGTGAC GCCCGATTCG TTCGACGCGT GCATCGCGGT GAACCTGCGC CATCAGTTCT TCGCCGCGCA GGCGGTGATC GACGACATGA AGCGGCTGGG CGGCGGCTCG ATCGTCAATC TCGGCTCGAT CAGCTGGATG CTGAAGAACG CCGGCTATCC GGTCTACGCG AGCGCGAAGG CGGCGGTGCA GGGGCTCACG CGCGCGCTCG CGCGCGAGCT CGGGCCGTTC GGCATTCGCG TGAATACGCT CGTGCCCGGC TGGGTGATGA CCGACAAGCA GCGTCGCTTG TGGCTCGACG ACGCGGGCCG CGCGGCGATC AAGGCGGGGC AGTGCATCGA CGCGGAGCTG CTTCCGGGCG ACCTCGCGCG GATGGCGCTC TTTCTCGCGG CGGACGACAG CCGGATGATC ACCGCGCAGG ACGTTGTCGT CGACGGCGGC TGGGCATGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T