SSGCID: Target Page

ButhA.00010.e

Oxidoreductase, short-chain dehydrogenase/reductase family

CENTER ID:	ButhA.00010.e
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00010.e.B1.GE36006	Full length( ButhA.00010.e )		1	264

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00010.e.B1.PS01721	Full length( ButhA.00010.e )		1	264

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.5228
RefSeq:	YP_442908.1
UniProt:	Q2SVZ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTGKRILVTG AGSGFGREVA LRLAAKGHHV IAGVQIAPQV TELNAEAARR GAALDAVKLD VTSARDRAQA ARWDIDVLLN NAGAGEAGAL ADLPVDIVRE LFETNVFGPL ELTQQVARGM IARGRGRIVF VSSIAGLITG AYTGAYCASK HAVEAIAEAM HAELAVHGIQ IAVVNPGPYS TGFNDRMMET PRRWLDPARD AATPEKLTFP LEQFDPQEMI EKMVEVTESE GGLFRNLLPA AAEQFVRDEQ AHAWTRLQNE RRGA

NT Sequence

ATGACTGGAA AACGCATTCT GGTGACGGGC GCGGGCTCGG GTTTCGGCCG CGAGGTCGCG CTGCGGCTTG CCGCGAAGGG CCACCACGTG ATCGCGGGCG TGCAGATCGC CCCGCAGGTC ACTGAGCTGA ACGCCGAGGC CGCGCGGCGC GGCGCGGCGC TCGACGCCGT GAAGCTCGAT GTGACCTCCG CGCGCGACCG CGCGCAGGCC GCGCGCTGGG ATATCGACGT GCTGCTGAAC AACGCGGGCG CGGGCGAGGC GGGCGCGCTC GCCGACCTGC CCGTCGATAT CGTTCGCGAG CTGTTCGAGA CGAACGTGTT CGGCCCGCTC GAGCTCACGC AGCAGGTCGC GCGCGGGATG ATCGCGCGCG GGCGCGGCAG GATCGTGTTC GTATCGTCGA TCGCGGGGCT CATCACGGGC GCGTACACGG GCGCGTATTG CGCGTCGAAG CATGCGGTCG AGGCGATCGC CGAGGCGATG CACGCGGAGC TTGCCGTGCA CGGCATTCAG ATCGCCGTCG TCAACCCGGG GCCGTACAGC ACGGGCTTCA ACGACAGGAT GATGGAGACG CCGCGCCGCT GGCTCGACCC GGCGCGCGAC GCGGCGACGC CCGAGAAGCT GACGTTCCCG CTCGAGCAGT TCGATCCGCA GGAGATGATC GAGAAGATGG TCGAAGTGAC CGAGAGCGAA GGCGGGCTGT TCCGCAACTT GCTGCCCGCC GCCGCGGAGC AGTTCGTGCG CGACGAGCAG GCGCACGCGT GGACGCGCCT TCAGAACGAA CGGCGCGGCG CG

Details for ButhA.00010.e.B1.GE36006

HARVESTED ON:	10/2/2012
SEQUENCED ON:	10/1/2012
EXPECTED MW:	29kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMT GKRILVTGAG SGFGREVALR LAAKGHHVIA GVQIAPQVTE LNAEAARRGA ALDAVKLDVT SARDRAQAAR WDIDVLLNNA GAGEAGALAD LPVDIVRELF ETNVFGPLEL TQQVARGMIA RGRGRIVFVS SIAGLITGAY TGAYCASKHA VEAIAEAMHA ELAVHGIQIA VVNPGPYSTG FNDRMMETPR RWLDPARDAA TPEKLTFPLE QFDPXEMIEK MVEVTESEGG LFRNLLPAAA EQFVRDEXAH AWTRLQNERR GA

Validated NT Sequence

ttttgtttac tttaagaagg aganatacca tggctcacca ccaccaccac catatgactg gaaaacgcat tctggtgacg ggcgcgggct cgggtttcgg ccgcgaggtc gcgctgcggc ttgccgcgaa gggccaccac gtgatcgcgg gcgtgcagat cgccccgcag gtcactgagc tgaacgccga ggccgcgcgg cgcggcgcgg cgctcgacgc cgtgaagctc gatgtgacct ccgcgcgcga ccgcgcgcag gccgcgcgct gggatatcga cgtgctgctg aacaacgcgg gcgcgggcga ggcgggcgcg ctcgccgacc tgcccgtcga tatcgttcgc gagctgttcg agacgaacgt gttcggcccg ctcgagctca cgcagcaggt cgcgcgcggg atgatcgcgc gcgggcgcgg caggatcgtg ttcgtatcgt cgatcgcggg gctcatcacg ggcgcgtaca cgggcgcgta ttgcgcgtcg aagcatgcgg tcgaggcgat cgccgaggcg atgcacgcgg agcttgccgt gcacggcatt cagatcgccg tcgtcaaccc ggggccgtac agcacgggct tcaacgacag gatgatggag acgccgcgcc gctggctcga cccggcgcgc gacgcggcga cgcccgagaa gctgacgttc ccgctcgagc agttcgatcc gcangagatg atcgagaaga tggtcgaagt gaccgagagc gaaggcgggc tgttccgcaa cttgctgccc gccgccgcgg agcagttcgt gcgcgacgag cangcgcacg cgtggacgcg ccttcagaac gaacggcgcg gcgcgtaagt gagtaagata ggatnnnnnn gctannanaa

Expected Protein Sequence

MAHHHHHHMT GKRILVTGAG SGFGREVALR LAAKGHHVIA GVQIAPQVTE LNAEAARRGA ALDAVKLDVT SARDRAQAAR WDIDVLLNNA GAGEAGALAD LPVDIVRELF ETNVFGPLEL TQQVARGMIA RGRGRIVFVS SIAGLITGAY TGAYCASKHA VEAIAEAMHA ELAVHGIQIA VVNPGPYSTG FNDRMMETPR RWLDPARDAA TPEKLTFPLE QFDPQEMIEK MVEVTESEGG LFRNLLPAAA EQFVRDEQAH AWTRLQNERR GA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactg gaaaacgcat tctggtgacg ggcgcgggct cgggtttcgg ccgcgaggtc gcgctgcggc ttgccgcgaa gggccaccac gtgatcgcgg gcgtgcagat cgccccgcag gtcactgagc tgaacgccga ggccgcgcgg cgcggcgcgg cgctcgacgc cgtgaagctc gatgtgacct ccgcgcgcga ccgcgcgcag gccgcgcgct gggatatcga cgtgctgctg aacaacgcgg gcgcgggcga ggcgggcgcg ctcgccgacc tgcccgtcga tatcgttcgc gagctgttcg agacgaacgt gttcggcccg ctcgagctca cgcagcaggt cgcgcgcggg atgatcgcgc gcgggcgcgg caggatcgtg ttcgtatcgt cgatcgcggg gctcatcacg ggcgcgtaca cgggcgcgta ttgcgcgtcg aagcatgcgg tcgaggcgat cgccgaggcg atgcacgcgg agcttgccgt gcacggcatt cagatcgccg tcgtcaaccc ggggccgtac agcacgggct tcaacgacag gatgatggag acgccgcgcc gctggctcga cccggcgcgc gacgcggcga cgcccgagaa gctgacgttc ccgctcgagc agttcgatcc gcaggagatg atcgagaaga tggtcgaagt gaccgagagc gaaggcgggc tgttccgcaa cttgctgccc gccgccgcgg agcagttcgt gcgcgacgag caggcgcacg cgtggacgcg ccttcagaac gaacggcgcg gcgcgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for ButhA.00010.e.B1.PS01721

PURIFICATION DATe:	1/15/2013
CONCENTRATION:	21.2mg/ml
OBSERVED MW:	28kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMT GKRILVTGAG SGFGREVALR LAAKGHHVIA GVQIAPQVTE LNAEAARRGA ALDAVKLDVT SARDRAQAAR WDIDVLLNNA GAGEAGALAD LPVDIVRELF ETNVFGPLEL TQQVARGMIA RGRGRIVFVS SIAGLITGAY TGAYCASKHA VEAIAEAMHA ELAVHGIQIA VVNPGPYSTG FNDRMMETPR RWLDPARDAA TPEKLTFPLE QFDPQEMIEK MVEVTESEGG LFRNLLPAAA EQFVRDEQAH AWTRLQNERR GA

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACTG GAAAACGCAT TCTGGTGACG GGCGCGGGCT CGGGTTTCGG CCGCGAGGTC GCGCTGCGGC TTGCCGCGAA GGGCCACCAC GTGATCGCGG GCGTGCAGAT CGCCCCGCAG GTCACTGAGC TGAACGCCGA GGCCGCGCGG CGCGGCGCGG CGCTCGACGC CGTGAAGCTC GATGTGACCT CCGCGCGCGA CCGCGCGCAG GCCGCGCGCT GGGATATCGA CGTGCTGCTG AACAACGCGG GCGCGGGCGA GGCGGGCGCG CTCGCCGACC TGCCCGTCGA TATCGTTCGC GAGCTGTTCG AGACGAACGT GTTCGGCCCG CTCGAGCTCA CGCAGCAGGT CGCGCGCGGG ATGATCGCGC GCGGGCGCGG CAGGATCGTG TTCGTATCGT CGATCGCGGG GCTCATCACG GGCGCGTACA CGGGCGCGTA TTGCGCGTCG AAGCATGCGG TCGAGGCGAT CGCCGAGGCG ATGCACGCGG AGCTTGCCGT GCACGGCATT CAGATCGCCG TCGTCAACCC GGGGCCGTAC AGCACGGGCT TCAACGACAG GATGATGGAG ACGCCGCGCC GCTGGCTCGA CCCGGCGCGC GACGCGGCGA CGCCCGAGAA GCTGACGTTC CCGCTCGAGC AGTTCGATCC GCAGGAGATG ATCGAGAAGA TGGTCGAAGT GACCGAGAGC GAAGGCGGGC TGTTCCGCAA CTTGCTGCCC GCCGCCGCGG AGCAGTTCGT GCGCGACGAG CAGGCGCACG CGTGGACGCG CCTTCAGAAC GAACGGCGCG GCGCGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT