SSGCID: Target Page

ButhA.00010.a

Oxidoreductase, short-chain dehydrogenase/reductase family

CENTER ID:	ButhA.00010.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00010.a.B1.GE36002	Full length( ButhA.00010.a )		1	257

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.5688
RefSeq:	YP_443329.1
UniProt:	Q2SUS0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MADHGIKGKT VIIAGGAKNL GGLIARDLAA QGAKAVVIHY NSPGTRADAE ATVAAVKAAG AHAVALQADL TTAGAVEKLF ADAVAAVGRP DIAINTVGKV LKKPFVEITE AEYDEMAAVN SKTAFFFLKE AGKHVNDNGK IVTLVTSLLG AFTPFYAAYA GMKAPVEHFT RAAAKEFGAR GVSVTAVGPG PMDTPFFYPA EGADAVAYHK TAAALSPFSK TGLTDIEDVV PFIRHLVSDG WWITGQTILI NGGYTTK

NT Sequence

ATGGCAGATC ACGGCATCAA GGGCAAAACC GTCATCATCG CGGGCGGCGC GAAGAACCTC GGCGGGCTGA TCGCGCGCGA TCTCGCCGCG CAAGGCGCGA AGGCGGTCGT GATCCACTAC AACAGCCCGG GTACGCGGGC AGACGCGGAA GCGACCGTCG CCGCGGTGAA GGCGGCGGGC GCGCACGCGG TCGCGCTGCA GGCGGACCTG ACGACGGCAG GCGCTGTCGA GAAGCTGTTC GCCGACGCGG TGGCCGCGGT CGGCCGCCCG GACATCGCAA TCAATACCGT CGGCAAGGTG CTGAAGAAGC CGTTCGTCGA GATCACCGAG GCCGAATACG ACGAGATGGC GGCCGTCAAT TCGAAGACGG CGTTCTTCTT CCTGAAGGAG GCGGGCAAGC ACGTGAACGA CAACGGCAAG ATCGTCACGC TCGTCACGTC GCTGCTCGGC GCGTTCACGC CGTTCTATGC GGCCTATGCG GGGATGAAGG CGCCTGTCGA GCACTTCACG CGCGCGGCCG CGAAGGAGTT CGGCGCGCGC GGCGTCTCGG TGACGGCGGT GGGGCCGGGC CCGATGGATA CGCCGTTCTT CTATCCGGCC GAAGGCGCGG ACGCGGTCGC GTACCACAAG ACGGCCGCGG CGCTGTCGCC GTTCTCGAAG ACGGGCCTGA CCGACATCGA GGACGTCGTG CCGTTCATCC GCCATCTCGT CAGCGACGGC TGGTGGATCA CCGGCCAGAC GATCCTGATC AACGGCGGTT ATACGACGAA G

Details for ButhA.00010.a.B1.GE36002

HARVESTED ON:	10/2/2012
SEQUENCED ON:	10/1/2012
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA DHGIKGKTVI IAGGAKNLGG LIARDLAAQG AKAVVIHYNS PGTRADAEAT VAAVKAAGAH AVALQADLTT AGAVEKLFAD AVAAVGRPDI AINTVGKVLK KPFVEITEAE YDEMAAVNSK TAFFFLKEAG KHVNDNGKIV TLVTSLLGAF TPFYAAYAGM KAPVEHFTRA AAKEFGARGV SVTAVGPGPM DTPFFYPAEG ADAVAYHKTA AALSPFSKTG LTDIEDVVPF IRHLVSDGWW ITGQTILING GYTTK

Validated NT Sequence

atcttactca cttacttcgt cgtataaccg ccgttgatca ggatcgtctg gccggtgatc caccagccgt cgctgacgag atggcggatg aacggcacga cgtcctcgat gtcggtcagg cccgtcttcg agaacggcga cagcgccgcg gccgtcttgt ggtacgcgac cgcgtccgcg ccttcggccg gatagaagaa cggcgtatcc atcgggcccg gccccaccgc cgtcaccgag acgccgcgcg cgccgaactc cttcgcggcc gcgcgcgtga agtgctcgac aggcgccttc atccccgcat aggccgcata gaacggcgtg aacgcgccga gcagcgacgt gacgagcgtg acgatcttgc cgttgtcgtt cacgtgcttg cccgcctcct tcaggaagaa gaacgccgtc ttcgaattga cggccgccat ctcgtcgtat tcggcctcgg tgatctcgac gaacggcttc ttcagcacct tgccgacggt attgattgcg atgtccgggc ggccgaccgc ggccaccgcg tcggcgaaca gcttctcgac agcgcctgcc gtcgtcaggt ccgcctgcag cgcgaccgcg tgcgcgcccg ccgccttcac cgcggcgacg gtcgcttccg cgtctgcccg cgtacccggg ctgttgtagt ggatcacgac cgccttcgcg ccttgcgcgg cgagatcgcg cgcgatcagc ccgccgaggt tcttcgcgcc gcccgcgatg atgacggttt tgcccttgat gccgtgatct gccatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcag atcacggcat caagggcaaa accgtcatca tcgcgggcgg cgcgaagaac ctcggcgggc tgatcgcgcg cgatctcgcc gcgcaaggcg cgaaggcggt cgtgatccac tacaacagcc cgggtacgcg ggcagacgcg gaagcgaccg tcgccgcggt gaaggcggcg ggcgcgcacg cggtcgcgct gcaggcggac ctgacgacgg caggcgctgt cgagaagctg ttcgccgacg cggtggccgc ggtcggccgc ccggacatcg caatcaatac cgtcggcaag gtgctgaaga agccgttcgt cgagatcacc gaggccgaat acgacgagat ggcggccgtc aattcgaaga cggcgttctt cttcctgaag gaggcgggca agcacgtgaa cgacaacggc aagatcgtca cgctcgtcac gtcgctgctc ggcgcgttca cgccgttcta tgcggcctat gcggggatga aggcgcctgt cgagcacttc acgcgcgcgg ccgcgaagga gttcggcgcg cgcggcgtct cggtgacggc ggtggggccg ggcccgatgg atacgccgtt cttctatccg gccgaaggcg cggacgcggt cgcgtaccac aagacggccg cggcgctgtc gccgttctcg aagacgggcc tgaccgacat cgaggacgtc gtgccgttca tccgccatct cgtcagcgac ggctggtgga tcaccggcca gacgatcctg atcaacggcg gttatacgac gaagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat