ButhA.00008.a

Serine hydroxymethyltransferase 1

CENTER ID: ButhA.00008.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00008.a.B1.GE36001 Full length( ButhA.00008.a ) 1 415
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00008.a.B1.PW36646 Full length( ButhA.00008.a ) 1 415

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.4154
RefSeq: YP_441925.2
UniProt: Q2SYS4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFDRAQSTIA NVDPEIWQAI QQENVRQEEH IELIASENYT SPAVMAAQGS QLTNKYAEGY PGKRYYGGCE YVDIVEQLAI DRVKALFGSE AANVQPNSGS QANQGVFFAM LKPGDTIMGM SLAHGGHLTH GSPVNMSGKW FNVVSYGLNE NEDIDYEAAD KLAHEHKPKL IVAGASAFAL KIDFERLAKI AKAVGAYLMV DMAHYAGLIA AGVYPNPVPH ADFVTTTTHK SLRGPRGGVI LMKAEYEKQI NSAIFPGIQG GPLMHVIAAK AVAFKEALSP EFKEYQQKVI ENARVLAETL VKRGLRIVSG RTESHVMLVD LRAKNITGKA AEAALGNAHI TVNKNAIPND PEKPFVTSGV RLGSPAMTTR GFGPQEAELV GNLIADVLEH PEDAATIERV RAQVADLTKR FPVYR
NT Sequence
ATGTTTGACA GAGCCCAAAG CACCATTGCG AACGTCGATC CCGAGATCTG GCAAGCGATC CAGCAGGAAA ACGTCCGCCA GGAAGAGCAC ATCGAGCTGA TCGCGTCGGA GAACTACACG AGCCCGGCCG TGATGGCGGC GCAAGGCTCG CAGCTCACGA ACAAGTACGC GGAAGGCTAC CCGGGCAAGC GCTATTACGG CGGCTGCGAA TACGTCGACA TCGTCGAGCA GCTTGCGATC GACCGCGTGA AGGCGCTGTT CGGCTCCGAA GCCGCGAACG TGCAGCCGAA CTCCGGCTCG CAGGCGAACC AGGGCGTGTT CTTCGCGATG CTCAAGCCGG GCGATACGAT CATGGGCATG AGCCTCGCGC ATGGCGGCCA CCTGACGCAC GGCTCGCCCG TCAACATGTC GGGCAAATGG TTCAACGTCG TGAGCTACGG CTTGAACGAG AACGAAGACA TCGACTACGA AGCCGCCGAT AAGCTTGCGC ACGAGCACAA GCCGAAGCTG ATCGTCGCCG GCGCTTCGGC GTTTGCGCTG AAGATCGATT TCGAGCGTCT CGCGAAGATC GCGAAGGCGG TCGGCGCGTA CCTGATGGTC GACATGGCGC ACTACGCGGG CCTCATCGCC GCTGGCGTCT ACCCGAACCC GGTGCCGCAC GCCGATTTCG TCACGACGAC GACGCACAAG AGCCTGCGCG GCCCGCGCGG CGGCGTGATC CTGATGAAGG CCGAGTACGA GAAGCAGATC AACTCGGCGA TCTTCCCGGG CATCCAGGGC GGTCCGCTGA TGCACGTGAT TGCGGCGAAG GCCGTCGCGT TCAAGGAAGC GCTGTCGCCG GAATTCAAGG AATACCAGCA GAAGGTGATC GAGAACGCGC GCGTGCTGGC CGAGACGCTC GTCAAGCGCG GCCTGCGCAT CGTGTCGGGC CGCACCGAAA GCCACGTGAT GCTCGTTGAC CTGCGCGCGA AGAACATTAC CGGCAAGGCC GCGGAAGCGG CGCTCGGCAA TGCGCACATC ACGGTCAACA AGAACGCGAT CCCGAACGAT CCGGAGAAGC CGTTCGTGAC GAGCGGCGTG CGTCTCGGCT CGCCCGCGAT GACGACGCGC GGCTTCGGTC CGCAGGAAGC CGAACTGGTC GGCAACCTGA TCGCCGACGT GCTCGAGCAT CCGGAAGACG CGGCGACGAT CGAGCGCGTG CGCGCGCAGG TCGCCGACCT GACCAAGCGT TTCCCGGTCT ATCGC
Details for ButhA.00008.a.B1.GE36001
HARVESTED ON: 10/2/2012
SEQUENCED ON: 10/1/2012
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 20
PERCENT COVERAGE: 91
Validated AA Sequence
MLEHVGDQVA DQFGFLRTEA ARRHRGRAET HAARHERLLR IVRDRVLVDR DVRIAERRFR GLAGNVLRAQ VNEHHVAFGA ARHDAQAALD ERLGQHARVL DHLLLVFLEF RRQRFLERDG LRRNHVHQRT ALDAREDRRV DLLLVLGLHQ DHAAARAAQA LVRRRRDEIG VRHRVRVDAS GDEARVVRHV DHQVRADRLR DLRETLEIDL QRKRRSAGDD QLRLVLVRKL IGGFVVDVFV LVQAVAHDVE PFARHVDGRA VRQVAAMREA HAHDRIARLE HREEHALVRL RAGVRLHVRG FGAEQRLHAV DRKLLDDVDV FAAAVIALAR VAFRVLVREL RALRRHHGRA RVVLRRDQLD VLFLADVFLL DRLPDLGIDV RNGALG
Validated NT Sequence
ggcgacctgc gcgcgcacgc gctcgatcgt cgccgcgtct tccggatgct cgagcacgtc ggcgatcagg ttgccgacca gttcggcttc ctgcggaccg aagccgcgcg tcgtcatcgc gggcgagccg agacgcacgc cgctcgtcac gaacggcttc tccggatcgt tcgggatcgc gttcttgttg accgtgatgt gcgcattgcc gagcgccgct tccgcggcct tgccggtaat gttcttcgcg cgcaggtcaa cgagcatcac gtggctttcg gtgcggcccg acacgatgcg caggccgcgc ttgacgagcg tctcggccag cacgcgcgcg ttctcgatca ccttctgctg gtattccttg aattccggcg acagcgcttc cttgaacgcg acggccttcg ccgcaatcac gtgcatcagc ggaccgccct ggatgcccgg gaagatcgcc gagttgatct gcttctcgta ctcggccttc atcaggatca cgccgccgcg cgggccgcgc aggctcttgt gcgtcgtcgt cgtgacgaaa tcggcgtgcg gcaccgggtt cgggtagacg ccagcggcga tgaggcccgc gtagtgcgcc atgtcgacca tcaggtacgc gccgaccgcc ttcgcgatct tcgcgagacg ctcgaaatcg atcttcagcg caaacgccga agcgccggcg acgatcagct tcggcttgtg ctcgtgcgca agcttatcgg cggcttcgta gtcgatgtct tcgttctcgt tcaagccgta gctcacgacg ttgaaccatt tgcccgacat gttgacgggc gagccgtgcg tcaggtggcc gccatgcgcg aggctcatgc ccatgatcgt atcgcccggc ttgagcatcg cgaagaacac gccctggttc gcctgcgagc cggagttcgg ctgcacgttc gcggcttcgg agccgaacag cgccttcacg cggtcgatcg caagctgctc gacgatgtcg acgtattcgc agccgccgta atagcgcttg cccgggtagc cttccgcgta cttgttcgtg agctgcgagc cttgcgccgc catcacggcc gggctcgtgt agttctccga cgcgatcagc tcgatgtgct cttcctggcg gacgttttcc tgctggatcg cttgccagat ctcgggatcg acgttcgcaa tggtgctttg ggctct
Expected Protein Sequence
MAHHHHHHMF DRAQSTIANV DPEIWQAIQQ ENVRQEEHIE LIASENYTSP AVMAAQGSQL TNKYAEGYPG KRYYGGCEYV DIVEQLAIDR VKALFGSEAA NVQPNSGSQA NQGVFFAMLK PGDTIMGMSL AHGGHLTHGS PVNMSGKWFN VVSYGLNENE DIDYEAADKL AHEHKPKLIV AGASAFALKI DFERLAKIAK AVGAYLMVDM AHYAGLIAAG VYPNPVPHAD FVTTTTHKSL RGPRGGVILM KAEYEKQINS AIFPGIQGGP LMHVIAAKAV AFKEALSPEF KEYQQKVIEN ARVLAETLVK RGLRIVSGRT ESHVMLVDLR AKNITGKAAE AALGNAHITV NKNAIPNDPE KPFVTSGVRL GSPAMTTRGF GPQEAELVGN LIADVLEHPE DAATIERVRA QVADLTKRFP VYR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtttg acagagccca aagcaccatt gcgaacgtcg atcccgagat ctggcaagcg atccagcagg aaaacgtccg ccaggaagag cacatcgagc tgatcgcgtc ggagaactac acgagcccgg ccgtgatggc ggcgcaaggc tcgcagctca cgaacaagta cgcggaaggc tacccgggca agcgctatta cggcggctgc gaatacgtcg acatcgtcga gcagcttgcg atcgaccgcg tgaaggcgct gttcggctcc gaagccgcga acgtgcagcc gaactccggc tcgcaggcga accagggcgt gttcttcgcg atgctcaagc cgggcgatac gatcatgggc atgagcctcg cgcatggcgg ccacctgacg cacggctcgc ccgtcaacat gtcgggcaaa tggttcaacg tcgtgagcta cggcttgaac gagaacgaag acatcgacta cgaagccgcc gataagcttg cgcacgagca caagccgaag ctgatcgtcg ccggcgcttc ggcgtttgcg ctgaagatcg atttcgagcg tctcgcgaag atcgcgaagg cggtcggcgc gtacctgatg gtcgacatgg cgcactacgc gggcctcatc gccgctggcg tctacccgaa cccggtgccg cacgccgatt tcgtcacgac gacgacgcac aagagcctgc gcggcccgcg cggcggcgtg atcctgatga aggccgagta cgagaagcag atcaactcgg cgatcttccc gggcatccag ggcggtccgc tgatgcacgt gattgcggcg aaggccgtcg cgttcaagga agcgctgtcg ccggaattca aggaatacca gcagaaggtg atcgagaacg cgcgcgtgct ggccgagacg ctcgtcaagc gcggcctgcg catcgtgtcg ggccgcaccg aaagccacgt gatgctcgtt gacctgcgcg cgaagaacat taccggcaag gccgcggaag cggcgctcgg caatgcgcac atcacggtca acaagaacgc gatcccgaac gatccggaga agccgttcgt gacgagcggc gtgcgtctcg gctcgcccgc gatgacgacg cgcggcttcg gtccgcagga agccgaactg gtcggcaacc tgatcgccga cgtgctcgag catccggaag acgcggcgac gatcgagcgc gtgcgcgcgc aggtcgccga cctgaccaag cgtttcccgg tctatcgctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for ButhA.00008.a.B1.PW36646
PURIFICATION DATe: 12/31/2012
CONCENTRATION: 39.9mg/ml
OBSERVED MW: 46kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 200µl
PERCENT IDENTITY: 20
PERCENT COVERAGE: 91
Protocol Notes
notes unavailable
Validated AA Sequence
MLEHVGDQVA DQFGFLRTEA ARRHRGRAET HAARHERLLR IVRDRVLVDR DVRIAERRFR GLAGNVLRAQ VNEHHVAFGA ARHDAQAALD ERLGQHARVL DHLLLVFLEF RRQRFLERDG LRRNHVHQRT ALDAREDRRV DLLLVLGLHQ DHAAARAAQA LVRRRRDEIG VRHRVRVDAS GDEARVVRHV DHQVRADRLR DLRETLEIDL QRKRRSAGDD QLRLVLVRKL IGGFVVDVFV LVQAVAHDVE PFARHVDGRA VRQVAAMREA HAHDRIARLE HREEHALVRL RAGVRLHVRG FGAEQRLHAV DRKLLDDVDV FAAAVIALAR VAFRVLVREL RALRRHHGRA RVVLRRDQLD VLFLADVFLL DRLPDLGIDV RNGALG
Validated NT Sequence
ggcgacctgc gcgcgcacgc gctcgatcgt cgccgcgtct tccggatgct cgagcacgtc ggcgatcagg ttgccgacca gttcggcttc ctgcggaccg aagccgcgcg tcgtcatcgc gggcgagccg agacgcacgc cgctcgtcac gaacggcttc tccggatcgt tcgggatcgc gttcttgttg accgtgatgt gcgcattgcc gagcgccgct tccgcggcct tgccggtaat gttcttcgcg cgcaggtcaa cgagcatcac gtggctttcg gtgcggcccg acacgatgcg caggccgcgc ttgacgagcg tctcggccag cacgcgcgcg ttctcgatca ccttctgctg gtattccttg aattccggcg acagcgcttc cttgaacgcg acggccttcg ccgcaatcac gtgcatcagc ggaccgccct ggatgcccgg gaagatcgcc gagttgatct gcttctcgta ctcggccttc atcaggatca cgccgccgcg cgggccgcgc aggctcttgt gcgtcgtcgt cgtgacgaaa tcggcgtgcg gcaccgggtt cgggtagacg ccagcggcga tgaggcccgc gtagtgcgcc atgtcgacca tcaggtacgc gccgaccgcc ttcgcgatct tcgcgagacg ctcgaaatcg atcttcagcg caaacgccga agcgccggcg acgatcagct tcggcttgtg ctcgtgcgca agcttatcgg cggcttcgta gtcgatgtct tcgttctcgt tcaagccgta gctcacgacg ttgaaccatt tgcccgacat gttgacgggc gagccgtgcg tcaggtggcc gccatgcgcg aggctcatgc ccatgatcgt atcgcccggc ttgagcatcg cgaagaacac gccctggttc gcctgcgagc cggagttcgg ctgcacgttc gcggcttcgg agccgaacag cgccttcacg cggtcgatcg caagctgctc gacgatgtcg acgtattcgc agccgccgta atagcgcttg cccgggtagc cttccgcgta cttgttcgtg agctgcgagc cttgcgccgc catcacggcc gggctcgtgt agttctccga cgcgatcagc tcgatgtgct cttcctggcg gacgttttcc tgctggatcg cttgccagat ctcgggatcg acgttcgcaa tggtgctttg ggctct
Expressed Protein Sequence
MAHHHHHHMF DRAQSTIANV DPEIWQAIQQ ENVRQEEHIE LIASENYTSP AVMAAQGSQL TNKYAEGYPG KRYYGGCEYV DIVEQLAIDR VKALFGSEAA NVQPNSGSQA NQGVFFAMLK PGDTIMGMSL AHGGHLTHGS PVNMSGKWFN VVSYGLNENE DIDYEAADKL AHEHKPKLIV AGASAFALKI DFERLAKIAK AVGAYLMVDM AHYAGLIAAG VYPNPVPHAD FVTTTTHKSL RGPRGGVILM KAEYEKQINS AIFPGIQGGP LMHVIAAKAV AFKEALSPEF KEYQQKVIEN ARVLAETLVK RGLRIVSGRT ESHVMLVDLR AKNITGKAAE AALGNAHITV NKNAIPNDPE KPFVTSGVRL GSPAMTTRGF GPQEAELVGN LIADVLEHPE DAATIERVRA QVADLTKRFP VYR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTTG ACAGAGCCCA AAGCACCATT GCGAACGTCG ATCCCGAGAT CTGGCAAGCG ATCCAGCAGG AAAACGTCCG CCAGGAAGAG CACATCGAGC TGATCGCGTC GGAGAACTAC ACGAGCCCGG CCGTGATGGC GGCGCAAGGC TCGCAGCTCA CGAACAAGTA CGCGGAAGGC TACCCGGGCA AGCGCTATTA CGGCGGCTGC GAATACGTCG ACATCGTCGA GCAGCTTGCG ATCGACCGCG TGAAGGCGCT GTTCGGCTCC GAAGCCGCGA ACGTGCAGCC GAACTCCGGC TCGCAGGCGA ACCAGGGCGT GTTCTTCGCG ATGCTCAAGC CGGGCGATAC GATCATGGGC ATGAGCCTCG CGCATGGCGG CCACCTGACG CACGGCTCGC CCGTCAACAT GTCGGGCAAA TGGTTCAACG TCGTGAGCTA CGGCTTGAAC GAGAACGAAG ACATCGACTA CGAAGCCGCC GATAAGCTTG CGCACGAGCA CAAGCCGAAG CTGATCGTCG CCGGCGCTTC GGCGTTTGCG CTGAAGATCG ATTTCGAGCG TCTCGCGAAG ATCGCGAAGG CGGTCGGCGC GTACCTGATG GTCGACATGG CGCACTACGC GGGCCTCATC GCCGCTGGCG TCTACCCGAA CCCGGTGCCG CACGCCGATT TCGTCACGAC GACGACGCAC AAGAGCCTGC GCGGCCCGCG CGGCGGCGTG ATCCTGATGA AGGCCGAGTA CGAGAAGCAG ATCAACTCGG CGATCTTCCC GGGCATCCAG GGCGGTCCGC TGATGCACGT GATTGCGGCG AAGGCCGTCG CGTTCAAGGA AGCGCTGTCG CCGGAATTCA AGGAATACCA GCAGAAGGTG ATCGAGAACG CGCGCGTGCT GGCCGAGACG CTCGTCAAGC GCGGCCTGCG CATCGTGTCG GGCCGCACCG AAAGCCACGT GATGCTCGTT GACCTGCGCG CGAAGAACAT TACCGGCAAG GCCGCGGAAG CGGCGCTCGG CAATGCGCAC ATCACGGTCA ACAAGAACGC GATCCCGAAC GATCCGGAGA AGCCGTTCGT GACGAGCGGC GTGCGTCTCG GCTCGCCCGC GATGACGACG CGCGGCTTCG GTCCGCAGGA AGCCGAACTG GTCGGCAACC TGATCGCCGA CGTGCTCGAG CATCCGGAAG ACGCGGCGAC GATCGAGCGC GTGCGCGCGC AGGTCGCCGA CCTGACCAAG CGTTTCCCGG TCTATCGCTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT