BupsM.19914.a

Acid phosphatase (acpA BP1026B_I3569)

CENTER ID: BupsM.19914.a
ORGANISM: Burkholderia pseudomallei 1026b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsM.19914.a.B1.GE42247 full length 1 553
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|884204.3.peg.3918
UniProt: A0A0H3HV11

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDKHPPSTD LPEDPDRRRV LGGIAALGAS VALAGCETAV PAAPRSAAEL RLDTALRREV RNIVVIYAEN RSFANLFGDF PGVQQPLGAV SPERYAQLDR DGKTPLATLP KIWGGLVPQA QVVDGKRYMI GEHDIVGLPN APFVIPDAQG KPLPNGVITR DLWHRFYQNQ MQINGGRNDQ FAAWADSGGL VMGHYRNSAE TLRLWNLARG YTLCDNFFMA AFGGSWLNHI FLISAQAPLY PDVHQSPAKH LVSVVEGDDP AGTRLKLAAD SPASALDGPP KFENDGLFTP DGYAVNTMAP PYQPSSVRPP VEGNPAYADP SNPRVLPPQH YATIGDRLSE KGVDWAWYGG AWQYALEHRD TGSVPDFQYH HQPFNYFASY APGTEARRRH LRDAGLGDDP STNHLIADID AGRLPTVTFY KPQGNLNMHA GYADVASGDR HIATVIEHIM RGPQWAHTVV ILTVDENGGW WDPVSPPKGD RWGPGSRIPA MVISPFAKKG YVDHTLYDTN SILRFISRVH GLAPLDGVLA RERAFAARGA QPPGDLTEAL DLG
NT Sequence
gtgactgaca agcatccccc gtcgaccgac cttcccgagg accccgatcg ccgccgcgtg ctcggcggca tcgcggcgct cggcgcgagc gtcgcgctcg ccggctgcga gacggccgtg cccgccgcgc cgcgctcggc cgccgaactg cggctcgaca ccgcgctgcg ccgcgaagtg cgcaacatcg tcgtgatcta cgcggagaat cgcagcttcg cgaacctgtt cggcgatttc cccggcgtgc agcagccgct cggcgcggta tcgcccgagc gctacgcgca gctcgaccgc gacggcaaga cgccgctcgc gacgctgccg aaaatctggg gcggcctcgt gccgcaggcg caagtcgtcg acggcaagcg ctacatgatc ggcgagcacg acattgtcgg gctgccgaac gcgccgttcg tgattcccga tgcgcagggc aagccgctgc cgaacggcgt gatcacgcgc gacttgtggc atcgcttcta tcagaaccag atgcagatca acggcggccg caacgaccag ttcgccgcct gggccgattc gggcggcctc gtgatggggc attaccgcaa ttcggccgag acgctgcggc tgtggaatct cgcgcgcggg tacacgctct gcgacaactt cttcatggcc gcgttcggcg gctcgtggct gaaccacatc ttcctcattt ccgcgcaggc gccgctttat ccggacgtgc accagagccc ggcgaagcat ctcgtatccg tcgtcgaagg cgacgatccg gccggcacgc ggctgaagct ggccgcggat tcgcccgcat cggcgctcga cggcccgccg aaattcgaga acgatggcct cttcacgccg gacggctacg cggtgaacac gatggcgccg ccgtatcagc cgagcagcgt gcgcccgccc gtcgaaggca atccggcgta cgcggacccg tcgaacccgc gcgtgctgcc gccgcagcat tacgcgacga tcggcgatcg cctgtcggag aagggcgtcg actgggcgtg gtacggcggt gcgtggcaat acgcgctcga gcatcgggac acgggctcgg tgccggactt ccagtatcac catcagccgt tcaattactt cgcgagctac gcgccgggca ccgaggcgcg gcgccggcac ctgcgcgacg cgggcctcgg cgacgatccg tcgacgaacc atctgatcgc cgacatcgac gcgggccgcc tgccgaccgt cacgttctac aagccgcagg gcaacctgaa catgcacgcg ggctatgcgg atgtcgcgtc gggcgaccgg cacatcgcga cggtgatcga gcacatcatg cgcggcccgc agtgggcgca cacggtcgtg atcctgacgg tcgacgagaa cggcggctgg tgggacccgg tgtcgccgcc gaagggcgac cggtgggggc cgggttcgcg gattccggcg atggtgatct cgccgttcgc gaagaagggc tacgtcgatc acacgctgta cgacacgaac tcgatcctgc gtttcatctc gcgcgtgcac gggctcgcgc cgctcgacgg cgtgctcgcg cgtgagcgcg cgttcgcggc gcgcggcgcg cagccgccgg gcgatctcac cgaagcgctc gacctcggct ga
Details for BupsM.19914.a.B1.GE42247
HARVESTED ON: 2/23/2018
SEQUENCED ON: 3/1/2018
EXPECTED MW: 61kDa
OBSERVED MW: 61kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 30
PERCENT COVERAGE: 96
Validated AA Sequence
MAHHHHHXMV XDXXPPSTDL PEDPDRXXXL XGXAALGASV XXAGXXXXXX AAPXXXAXLX XXXALXXXXX XXVVIXXXXP XXXNLXXDFP XVQXXLGAXX PERXXXXDRX XXXPLATLPX XWGGLXPXXQ VXXXXRXXXG XXXXXXXXNA XXXIPDAQXX XLPXGVITRD XXHXXXXXXX XXXGXXNXXX XAXXDSGGLX XXXXXXXXXX LXXXNLARXX XXXXXXXXXX XXXXXXXHIX XXSXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXIXXXXXX XXXERWXXXX XXXRXXXXXX XVSXEXXXPA RXXQXXVRGP XXPARXXAAA XRDDRRSPVG EGRRLGVVRR CVAIRARASG HGLGAGLPVS PSAVQLLREL RAGHRGAAPA PARRGPRRRS VDEPSDRRHR RGPPADRHVL QAAGQPEHAR GLCGCRVGRP AHRDGDRAHH ARPAVGAHGR DPDGRRERRL VGPGVAAEGR PVGAGFADSG DGDLAVREEG LRRSHAVRHE LDPAFHLARA RARAARRRAR A
Validated NT Sequence
gagancgccc ggcggctgcg cgccgcgcgc cgcgaacgcg cgctcacgcg cgagcacgcc gtcgagcggc gcgagcccgt gcacgcgcga gatgaaacgc aggatcgagt tcgtgtcgta cagcgtgtga tcgacgtagc ccttcttcgc gaacggcgag atcaccatcg ccggaatccg cgaacccggc ccccaccggt cgcccttcgg cggcgacacc gggtcccacc agccgccgtt ctcgtcgacc gtcaggatca cgaccgtgtg cgcccactgc gggccgcgca tgatgtgctc gatcaccgtc gcgatgtgcc ggtcgcccga cgcgacatcc gcatagcccg cgtgcatgtt caggttgccc tgcggcttgt agaacgtgac ggtcggcagg cggcccgcgt cgatgtcggc gatcagatgg ttcgtcgacg gatcgtcgcc gaggcccgcg tcgcgcaggt gccggcgccg cgcctcggtg cccggcgcgt agctcgcgaa gtaattgaac ggctgatggt gatactggaa gtccggcacc gagcccgtgt cccgatgctc gagcgcgtat tgccacgcac cgccgtacca cgcccagtcg acgcccttct ccgacaggcg atcgccgatc gtcgcgtann gctgcggcgg nnncncgcgc gggttnnncg ggtccgcgta cgcnnnnttg ccntnnncgg gcgggcgnnn nnngctcnnc tgatacnnnn nnnnnnnnnn nnnnnnnncg nnnnnnnnnn ncgnnnnnnn gccatcgttc nnnnnnnnnn nngnnnnnnn nnnnnnngat gnnnnnnnnn nnnnnnnnnn nnnncnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngnnn nnnnnnngnn ngnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggaaannnnn nngatgtggt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgcgcg cgagattcnn nannnncagc nnnncnnnnn nnnnnnnnnn nnnnnccann nngaggccgc ccgaatcgnn nnaggcggnn nnnnnnncgt tncngnnncc nnnnnnnnnn nnnnnnnnnn nnnngnnnnn atgnnnnnng tcgcgcgtga tcacgccgtn nggcagcgnn nnnncctgcg catcgggaat cnnnnnnngc gcgttcngna ncncgnnnnn nnnnngctng ccnatcnnnn ngcgnnnnnc gnnnnngact tgcgnnngcg gnnngaggcc gccccagann ntcggcagcg tcgcgagcgg cgnnnnncnn tcgcggtcgn nnngcgnnta gcgctcgggc gnnnnngcgc cgagnnnnnn ctgcacgncg gggaaatcgn nnnnnaggtt cnngannnnn ggnnnnnnnn nntagatcac gacnnnnnnn nnnnnttnnn gnnncagcgc gnnntcgann nncagnnngg cggncnannn cggcgcggcg gnnnnnnnnn nnnnnnngcc ggcgnnnnng acgctcgcgc cgagcgccgc gnnnccncnn agnncgcnnn ggcgatcggg gtcctcggga aggtcggtcg acgggggnnn cntgtcannn accatnnggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMT DKHPPSTDLP EDPDRRRVLG GIAALGASVA LAGCETAVPA APRSAAELRL DTALRREVRN IVVIYAENRS FANLFGDFPG VQQPLGAVSP ERYAQLDRDG KTPLATLPKI WGGLVPQAQV VDGKRYMIGE HDIVGLPNAP FVIPDAQGKP LPNGVITRDL WHRFYQNQMQ INGGRNDQFA AWADSGGLVM GHYRNSAETL RLWNLARGYT LCDNFFMAAF GGSWLNHIFL ISAQAPLYPD VHQSPAKHLV SVVEGDDPAG TRLKLAADSP ASALDGPPKF ENDGLFTPDG YAVNTMAPPY QPSSVRPPVE GNPAYADPSN PRVLPPQHYA TIGDRLSEKG VDWAWYGGAW QYALEHRDTG SVPDFQYHHQ PFNYFASYAP GTEARRRHLR DAGLGDDPST NHLIADIDAG RLPTVTFYKP QGNLNMHAGY ADVASGDRHI ATVIEHIMRG PQWAHTVVIL TVDENGGWWD PVSPPKGDRW GPGSRIPAMV ISPFAKKGYV DHTLYDTNSI LRFISRVHGL APLDGVLARE RAFAARGAQP PGDLTEALDL G
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgactg acaagcatcc cccgtcgacc gaccttcccg aggaccccga tcgccgccgc gtgctcggcg gcatcgcggc gctcggcgcg agcgtcgcgc tcgccggctg cgagacggcc gtgcccgccg cgccgcgctc ggccgccgaa ctgcggctcg acaccgcgct gcgccgcgaa gtgcgcaaca tcgtcgtgat ctacgcggag aatcgcagct tcgcgaacct gttcggcgat ttccccggcg tgcagcagcc gctcggcgcg gtatcgcccg agcgctacgc gcagctcgac cgcgacggca agacgccgct cgcgacgctg ccgaaaatct ggggcggcct cgtgccgcag gcgcaagtcg tcgacggcaa gcgctacatg atcggcgagc acgacattgt cgggctgccg aacgcgccgt tcgtgattcc cgatgcgcag ggcaagccgc tgccgaacgg cgtgatcacg cgcgacttgt ggcatcgctt ctatcagaac cagatgcaga tcaacggcgg ccgcaacgac cagttcgccg cctgggccga ttcgggcggc ctcgtgatgg ggcattaccg caattcggcc gagacgctgc ggctgtggaa tctcgcgcgc gggtacacgc tctgcgacaa cttcttcatg gccgcgttcg gcggctcgtg gctgaaccac atcttcctca tttccgcgca ggcgccgctt tatccggacg tgcaccagag cccggcgaag catctcgtat ccgtcgtcga aggcgacgat ccggccggca cgcggctgaa gctggccgcg gattcgcccg catcggcgct cgacggcccg ccgaaattcg agaacgatgg cctcttcacg ccggacggct acgcggtgaa cacgatggcg ccgccgtatc agccgagcag cgtgcgcccg cccgtcgaag gcaatccggc gtacgcggac ccgtcgaacc cgcgcgtgct gccgccgcag cattacgcga cgatcggcga tcgcctgtcg gagaagggcg tcgactgggc gtggtacggc ggtgcgtggc aatacgcgct cgagcatcgg gacacgggct cggtgccgga cttccagtat caccatcagc cgttcaatta cttcgcgagc tacgcgccgg gcaccgaggc gcggcgccgg cacctgcgcg acgcgggcct cggcgacgat ccgtcgacga accatctgat cgccgacatc gacgcgggcc gcctgccgac cgtcacgttc tacaagccgc agggcaacct gaacatgcac gcgggctatg cggatgtcgc gtcgggcgac cggcacatcg cgacggtgat cgagcacatc atgcgcggcc cgcagtgggc gcacacggtc gtgatcctga cggtcgacga gaacggcggc tggtgggacc cggtgtcgcc gccgaagggc gaccggtggg ggccgggttc gcggattccg gcgatggtga tctcgccgtt cgcgaagaag ggctacgtcg atcacacgct gtacgacacg aactcgatcc tgcgtttcat ctcgcgcgtg cacgggctcg cgccgctcga cggcgtgctc gcgcgtgagc gcgcgttcgc ggcgcgcggc gcgcagccgc cgggcgatct caccgaagcg ctcgacctcg gctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat