SSGCID: Target Page

BupsM.19910.a

Uncharacterized protein (BP1026B_I1564)

CENTER ID:	BupsM.19910.a
ORGANISM:	Burkholderia pseudomallei 1026b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsM.19910.a.B1.GE42243	full length		1	196

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|884204.3.peg.1737
UniProt:	A0A0H3HII7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDSAAEYGKV MQAIAANESA AVTYVQDSRL SATEVQTTHP IWSGVQNGDR LGVSWSGGDF QHQRIFVYEG NGNAGKWKKA EAADIPLKQG GAPSILNQTP LMPLMPQQQV RETSASAKSV ADVVSVSLYR RVTGNIDIYT VKVGSRIQGI YGVGMTAPGW IQADGVGTDI VVFQVRASGT PGLFYSGVAS ATVIRE

NT Sequence

atggattcgg cggccgaata cggaaaggtg atgcaggcca tcgccgcaaa cgaaagcgcc gctgtcacgt atgtgcaaga cagccgcctg tcggccaccg aggttcaaac gacgcatccg atctggagcg gcgtacagaa tggcgacagg ctcggcgtca gctggagcgg cggtgatttt cagcatcaac ggatcttcgt ctacgaaggc aacggcaatg ccgggaagtg gaaaaaagcc gaggcggcgg acattccgct caagcagggc ggagcgcctt ccatcctgaa tcagacgccg ctgatgccgc tgatgccgca gcagcaggtg cgcgaaacgt ccgcgtcggc caagagtgtc gcggatgtcg tttccgtgtc gctatatcga cgagtgactg gaaatatcga tatctatacg gtaaaagtgg gcagcaggat tcaaggcatt tacggtgtcg gcatgaccgc tcccggctgg attcaagccg acggcgtcgg aacggatatc gtcgtatttc aggtccgcgc ctccgggaca cccggtttgt tttatagcgg cgtggcgagc gccaccgtca tccgcgagtg a

Details for BupsM.19910.a.B1.GE42243

HARVESTED ON:	2/23/2018
SEQUENCED ON:	3/1/2018
EXPECTED MW:	22kDa
OBSERVED MW:	22kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMD SAAEYGKVMQ AIAANESAAV TYVQDSRLSA TEVQTTHPIW SGVQNGDRLG VSWSGGDFQH QRIFVYEGNG NAGKWKKAEA ADIPLKQGGA PSILNQTPLM PLMPQQQVRE TSASAKSVAD VVSVSLYRRV TGNIDIYTVK VGSRIQGIYG VGMTAPGWIQ ADGVGTDIVV FQVRASGTPG LFYSGVASAT VIRE

Validated NT Sequence

atcttactca cttactcgcg gatgacggtg gcgctcgcca cgccgctata aaacaaaccg ggtgtcccgg aggcgcggac ctgaaatacg acgatatccg ttccgacgcc gtcggcttga atccagccgg gagcggtcat gccgacaccg taaatgcctt gaatcctgct gcccactttt accgtataga tatcgatatt tccagtcact cgtcgatata gcgacacgga aacgacatcc gcgacactct tggccgacgc ggacgtttcg cgcacctgct gctgcggcat cagcggcatc agcggcgtct gattcaggat ggaaggcgct ccgccctgct tgagcggaat gtccgccgcc tcggcttttt tccacttccc ggcattgccg ttgccttcgt agacgaagat ccgttgatgc tgaaaatcac cgccgctcca gctgacgccg agcctgtcgc cattctgtac gccgctccag atcggatgcg tcgtttgaac ctcggtggcc gacaggcggc tgtcttgcac atacgtgaca gcggcgcttt cgtttgcggc gatggcctgc atcacctttc cgtattcggc cgccgaatcc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatt cggcggccga atacggaaag gtgatgcagg ccatcgccgc aaacgaaagc gccgctgtca cgtatgtgca agacagccgc ctgtcggcca ccgaggttca aacgacgcat ccgatctgga gcggcgtaca gaatggcgac aggctcggcg tcagctggag cggcggtgat tttcagcatc aacggatctt cgtctacgaa ggcaacggca atgccgggaa gtggaaaaaa gccgaggcgg cggacattcc gctcaagcag ggcggagcgc cttccatcct gaatcagacg ccgctgatgc cgctgatgcc gcagcagcag gtgcgcgaaa cgtccgcgtc ggccaagagt gtcgcggatg tcgtttccgt gtcgctatat cgacgagtga ctggaaatat cgatatctat acggtaaaag tgggcagcag gattcaaggc atttacggtg tcggcatgac cgctcccggc tggattcaag ccgacggcgt cggaacggat atcgtcgtat ttcaggtccg cgcctccggg acacccggtt tgttttatag cggcgtggcg agcgccaccg tcatccgcga gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat