BupsM.19909.a

Mechanosensitive ion channel family protein (BP1026B_I1537)

CENTER ID: BupsM.19909.a
ORGANISM: Burkholderia pseudomallei 1026b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsM.19909.a.B2.GE42242 MscS-like soluble domain 647 803
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|884204.3.peg.1710
UniProt: A0A0H3HJR4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIRLATFARH VALIGLLHLL CAALPAAAAD LASGASVPPA PAISRDEALA ELKRVQAALD RIKQQASAAT TYKQLDALDE STQALSADVD KLTAALVPTR AQLQAQLDVL GPPPAPGAAP ETPAVARQRA DLNARKTQLD AALKQAADEK ESLANLTQQY SKLRRSLLRD QLVLRSSSIL GPRFWAPLFR PSDDDRQELG AFSAQIGQVL AAAWEPGRRL GTALLLAAAL AVWTLGRRLA ERALAWFSLT KLPETRVRRS ALATSIALSS VVTTGIAVQF VYLALTRDYA LSSALQDFAD ELAKLALTCA LIAGLGRALL CTHHPSWRLP ALADEVALAM RPFPAILAAL LLVAGALEEL NRTADTSLQV TLFGRGIVSL VVVVTIGASL LRANRARTAL AAAGEPPEAR STLAGLIHAG VTLTVVAALA ALLIGYISVA RFLTYELVWF EIVIGSVYLL TRLTRDAYAS AFAANLSSGR LIKHLFGLND SHLDQARTVL TGISTSVLLL VAVIALLTGG FGTTPNDLLV SVISMFGGDK LQRLNIVPAR ILNAALALAI GIYLLRSVRR WLDNELLPAI GLDQGMRASL VTLFSNLGYV ALVLLALSLL GVKWDNLAWI VSALSVGIGF GLQEIVKNFV SGLILLTERP VKVGDMISIS GVEGDIRRIN VRATEIQLSD RSTVIVPNSQ LISQNLRNVT MGNSTQGVAT LMLTFPLNTD PEQVRDLLLD AYREHASVLE KPAPSVTFSQ LTPDGITLSV TGYVGSPRIA AATKSDLLFE ILKRLRAAGI ALSNPQTLMV QNLPPPSSE
NT Sequence
atgatccgtc ttgcgacatt cgcgcgccac gtcgcgctga taggcctatt gcatctgctc tgcgcggcgt tgcccgcggc ggcggccgac ctcgcgtccg gcgcgagcgt gccgcccgcg ccggcgatct cgcgtgacga ggcgctcgcc gaactgaagc gcgtgcaggc cgcgctcgac cggatcaagc agcaggcgtc ggccgcgacc acctacaagc aactggacgc gctcgacgaa tcgacgcaag cgctgagcgc cgacgtcgac aagctgacgg cggcgctcgt gcccacgcgc gcgcagcttc aggcgcagct cgacgtgctc ggaccgccgc ccgcgccggg cgcggcgccc gaaacgcccg ccgtcgcgcg gcagcgcgcg gacctgaacg cgcgcaagac gcagctcgac gccgcgctca agcaggccgc cgacgagaag gaaagcctcg cgaacctgac acagcaatac tcgaagctgc gccgcagcct gctgcgcgac cagctcgtgc tgcgctcgag cagcattctc ggcccgcggt tctgggcccc gctctttcgt ccgtcggacg atgatcggca ggaactcggc gcgttcagcg cgcagatcgg gcaagtgctt gccgcggcat gggagccggg ccgccggctc ggcaccgcgc tgctgctcgc cgccgcgctc gccgtgtgga cgctcggcag gcggctcgcc gagcgcgcgc tcgcgtggtt cagcctgacg aagctgccgg agacgcgcgt gcggcgcagc gcgctcgcga cgtcgatcgc gctgtcgagc gtcgtgacga cgggcatcgc cgtgcaattc gtctatctcg cgctcacgcg cgactacgcg ctgtcgagcg cgctgcagga tttcgccgac gagctcgcga agctcgcgct gacttgcgcg ctgatcgcgg ggctcgggcg cgcgctgctg tgcacccacc atccgagctg gcggctgccc gcgcttgccg acgaagtcgc gctcgcgatg cggccgttcc cggcgatcct cgcggcgctg ctgctcgtcg cgggcgcgct agaggaactg aaccgaaccg ccgacacgag cctgcaggtc acgctgttcg ggcgcggaat cgtgtcgctc gtcgtcgtcg tgacgatcgg cgcgtcgctg ctgcgcgcga accgcgcgcg caccgcgctc gccgccgcgg gcgagccgcc cgaggcgcgc tcgacgctcg cggggctgat ccacgcgggc gtcacgctga ccgtcgtcgc ggcgctcgcc gcgctcctca tcggctacat ctcggtcgcg cgctttctca cctatgagct cgtgtggttc gagatcgtga tcggcagcgt gtacttgctg acgcgcctca cccgcgacgc atacgcgagc gcgttcgccg cgaacctgtc gagcggccgg ctgatcaagc atctgttcgg gctgaacgac agccacctcg atcaggcacg caccgtgctg accggaatca gcacgagcgt gctgctgctc gtcgccgtca tcgcgctgct gacgggcggc ttcggcacga cgccgaacga tctgctcgtg agcgtgatct cgatgttcgg cggcgacaaa ctgcaacgcc tgaacatcgt gcccgcccgc atcctgaacg cggcgctcgc gctggcgatc ggcatctatc tgctgcgctc ggtgcgccgc tggctggaca acgagctgct gccggcgatc ggcctggatc agggcatgcg cgcgtcgctc gtcacgctgt tcagcaatct cggctatgtc gcgctcgtgc tgctcgcgct gtcgctgctc ggcgtcaaat gggacaacct cgcgtggatc gtgagcgcgc tgtcggtggg catcggcttc ggcctgcagg agatcgtgaa gaacttcgtg tccgggctga tcctgctcac cgagcggccc gtgaaggtcg gcgacatgat cagcatctcg ggcgtcgaag gcgacatccg ccggatcaac gtgcgcgcga ccgagatcca gctgtccgac cgctcgacgg tgatcgtgcc gaactcgcaa ttgatctcgc agaacctgcg taacgtgacg atgggcaaca gcacgcaggg cgtcgcgacg ctgatgctca cgtttccgct caacaccgat cccgagcagg tgcgcgatct gctgctcgac gcataccgcg agcacgcgtc ggtgctcgag aagcccgcgc cgtccgtcac cttcagccag ctgacgccgg acgggatcac gctcagcgtg acggggtacg tcggcagccc gcggatcgcg gcggcgacga agagcgacct gctgttcgag attctcaagc gactgcgcgc ggcgggcatc gcgctgtcga atccgcagac gctgatggtg cagaatctgc cgccgccttc gagcgagtga
Details for BupsM.19909.a.B2.GE42242
HARVESTED ON: 2/23/2018
SEQUENCED ON: 3/1/2018
EXPECTED MW: 18kDa
OBSERVED MW: 18kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT ERPVKVGDMI SISGVEGDIR RINVRATEIQ LSDRSTVIVP NSQLISQNLR NVTMGNSTQG VATLMLTFPL NTDPEQVRDL LLDAYREHAS VLEKPAPSVT FSQLTPDGIT LSVTGYVGSP RIAAATKSDL LFEILKRLRA AGIALSNPQT LMVQNL
Validated NT Sequence
atcttactca cttacagatt ctgcaccatc agcgtctgcg gattcgacag cgcgatgccc gccgcgcgca gtcgcttgag aatctcgaac agcaggtcgc tcttcgtcgc cgccgcgatc cgcgggctgc cgacgtaccc cgtcacgctg agcgtgatcc cgtccggcgt cagctggctg aaggtgacgg acggcgcggg cttctcgagc accgacgcgt gctcgcggta tgcgtcgagc agcagatcgc gcacctgctc gggatcggtg ttgagcggaa acgtgagcat cagcgtcgcg acgccctgcg tgctgttgcc catcgtcacg ttacgcaggt tctgcgagat caattgcgag ttcggcacga tcaccgtcga gcggtcggac agctggatct cggtcgcgcg cacgttgatc cggcggatgt cgccttcgac gcccgagatg ctgatcatgt cgccgacctt cacgggccgc tcggtcatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHTE RPVKVGDMIS ISGVEGDIRR INVRATEIQL SDRSTVIVPN SQLISQNLRN VTMGNSTQGV ATLMLTFPLN TDPEQVRDLL LDAYREHASV LEKPAPSVTF SQLTPDGITL SVTGYVGSPR IAAATKSDLL FEILKRLRAA GIALSNPQTL MVQNL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataccgagc ggcccgtgaa ggtcggcgac atgatcagca tctcgggcgt cgaaggcgac atccgccgga tcaacgtgcg cgcgaccgag atccagctgt ccgaccgctc gacggtgatc gtgccgaact cgcaattgat ctcgcagaac ctgcgtaacg tgacgatggg caacagcacg cagggcgtcg cgacgctgat gctcacgttt ccgctcaaca ccgatcccga gcaggtgcgc gatctgctgc tcgacgcata ccgcgagcac gcgtcggtgc tcgagaagcc cgcgccgtcc gtcaccttca gccagctgac gccggacggg atcacgctca gcgtgacggg gtacgtcggc agcccgcgga tcgcggcggc gacgaagagc gacctgctgt tcgagattct caagcgactg cgcgcggcgg gcatcgcgct gtcgaatccg cagacgctga tggtgcagaa tctgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat