BupsM.18900.c

Uncharacterized protein

CENTER ID: BupsM.18900.c
ORGANISM: Burkholderia pseudomallei 1026b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsM.18900.c.B2.GE38820 soluble domain 269 462
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|884204.6.peg.6912
RefSeq: YP_006276833.1
RefSeq: WP_004551205.1
UniProt: A0A0H3HU95

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDGFLIQRPS RLQTLGAGTF ALLTLGALVA TAPHADLRLP PIEPFLPMCA LTVFSTSLIA AFLLGAQFIV MRRPILGTLA GAYAFAAITV ALQLLAFPGV FAPTGLLGAR HESAAWLWTF WHGGFPLFVV LAMLTRYRLA KRLSNTRHVG RWLATLAGVP IAVAIALGLV ALRADLPAPF GPSLDNALLA ADPVAIVVWT INLGAIAAAL FVGRMRTMLD IWLVVVLLAG FTDTSLNLLS TARFTAGWYV ARLFSMIAPG ILVCVLVWEV TVLYRRLFDA HASLMRTASH DALTMVYNRS YFNEQFERAF HYARRHNTPL ALVMVDVDHF KQYNDTFGHL QGDTCLAAVA AALSGVAGRA NDFVARYGGE EFAVVLPGAD ERGAATIAER ARQAVERLRI AADTAPGYVT VSAGYAASSG NPFETPGALI AAADAALYRA KMLGRNVSAA PHHDMQDTAM RD
NT Sequence
atggatggtt tcctcattca acgcccgtct cgcctgcaga cgcttggggc cggaacattc gcgttgctga cgctgggcgc gctcgtggcc accgcgccgc acgcggatct gcggctgccg ccgatcgagc cgttcctgcc gatgtgcgcg ctgaccgtgt tcagcacgtc gctcatcgcg gccttcctgc tcggcgcgca gttcatcgtg atgcgccgcc cgattctcgg cacgctggcc ggcgcgtatg ccttcgcggc gatcacggtc gcgcttcaac tgctcgcgtt tcccggcgtc ttcgcgccca ccggcctgct cggcgcgcgg catgagagcg ccgcgtggct atggaccttc tggcacggcg gcttcccgct gttcgtcgtg ctggcgatgc tgacgcgata ccggctcgcg aagcggctca gcaacacccg ccacgtcggc cgctggctcg cgacattggc cggcgtgccg atcgcggtcg cgatcgcgct cggcctcgtc gcgctgcgcg cggatctgcc cgcgccgttc ggcccgtcgc tcgacaatgc gctgctcgcc gccgatccgg tggcgatcgt cgtctggacg atcaacctcg gcgcgatcgc cgccgcgctg ttcgtcggcc ggatgaggac gatgctcgac atctggctcg tcgtcgtgct gctcgccggc ttcaccgata ccagcctgaa tctgctgagc accgcgcgct tcaccgccgg ctggtacgtc gcgcgcctgt tcagcatgat cgcgccgggc atcctcgtct gcgtgctcgt ctgggaagtg acggtgctgt accggcggct gttcgacgcg cacgcatcgc tcatgcggac cgcttcgcac gacgcgctga cgatggtgta caaccgcagc tatttcaacg aacagttcga gcgcgccttt cactacgcac gccggcacaa cacgcccctc gcgctcgtga tggtcgacgt cgatcatttc aagcagtaca acgatacgtt cggccacctg caaggggaca cgtgcctcgc cgccgtcgcg gccgcgctgt ccggcgtcgc gggccgtgcg aacgatttcg tcgcgcgcta cggcggcgag gaattcgccg tcgtgctgcc cggggcggac gaacgcggcg cggcgacgat cgccgaacgg gcacggcagg ccgtcgagcg gctgcgcatc gccgccgaca cggcgcccgg ctatgtgacg gtgagcgccg gctacgcggc gagcagcggc aatccgttcg aaacgcccgg cgcgctgatc gcggccgccg acgcggcgct ctaccgcgcg aagatgctgg ggcgcaacgt cagcgccgcg ccccaccacg acatgcagga tacggcgatg cgcgactga
Details for BupsM.18900.c.B2.GE38820
HARVESTED ON: 1/22/2015
SEQUENCED ON: 1/15/2015
EXPECTED MW: 22kDa
OBSERVED MW: 22kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHME VTVLYRRLFD AHASLMRTAS HDALTMVYNR SYFNEQFERA FHYARRHNTP LALVMVDVDH FKQYNDTFGH LQGDTCLAAV AAALSGVAGR ANDFVARYGG EEFAVVLPGA DERGAATIAE RARQAVERLR IAADTAPGYV TVSAGYAASS GNPFETPGAL IAAADAALYR AKMLGRNVSA APHHDMQDTA MRD
Validated NT Sequence
atggctcacc accaccacca ccatatggaa gtgacggtgc tgtaccggcg gctgttcgac gcgcacgcat cgctcatgcg gaccgcttcg cacgacgcgc tgacgatggt gtacaaccgc agctatttca acgaacagtt cgagcgcgcc tttcactacg cacgccggca caacacgccc ctcgcgctcg tgatggtcga cgtcgatcat ttcaagcagt acaacgatac gttcggccac ctgcaagggg acacgtgcct cgccgccgtc gcggccgcgc tgtccggcgt cgcgggccgt gcgaacgatt tcgtcgcgcg ctacggcggc gaggaattcg ccgtcgtgct gcccggggcg gacgaacgcg gcgcggcgac gatcgccgaa cgggcacggc aggccgtcga gcggctgcgc atcgccgccg acacggcgcc cggctatgtg acggtgagcg ccggctacgc ggcgagcagc ggcaatccgt tcgaaacgcc cggcgcgctg atcgcggccg ccgacgcggc gctctaccgc gcgaagatgc tggggcgcaa cgtcagcgcc gcgccccacc acgacatgca ggatacggcg atgcgcgact aagtgagtaa gat
Expected Protein Sequence
MAHHHHHHEV TVLYRRLFDA HASLMRTASH DALTMVYNRS YFNEQFERAF HYARRHNTPL ALVMVDVDHF KQYNDTFGHL QGDTCLAAVA AALSGVAGRA NDFVARYGGE EFAVVLPGAD ERGAATIAER ARQAVERLRI AADTAPGYVT VSAGYAASSG NPFETPGALI AAADAALYRA KMLGRNVSAA PHHDMQDTAM RD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaagtga cggtgctgta ccggcggctg ttcgacgcgc acgcatcgct catgcggacc gcttcgcacg acgcgctgac gatggtgtac aaccgcagct atttcaacga acagttcgag cgcgcctttc actacgcacg ccggcacaac acgcccctcg cgctcgtgat ggtcgacgtc gatcatttca agcagtacaa cgatacgttc ggccacctgc aaggggacac gtgcctcgcc gccgtcgcgg ccgcgctgtc cggcgtcgcg ggccgtgcga acgatttcgt cgcgcgctac ggcggcgagg aattcgccgt cgtgctgccc ggggcggacg aacgcggcgc ggcgacgatc gccgaacggg cacggcaggc cgtcgagcgg ctgcgcatcg ccgccgacac ggcgcccggc tatgtgacgg tgagcgccgg ctacgcggcg agcagcggca atccgttcga aacgcccggc gcgctgatcg cggccgccga cgcggcgctc taccgcgcga agatgctggg gcgcaacgtc agcgccgcgc cccaccacga catgcaggat acggcgatgc gcgactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat