SSGCID: Target Page

BupsM.18900.a

GGDEF domain-containing protein

CENTER ID:	BupsM.18900.a
ORGANISM:	Burkholderia pseudomallei 1026b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsM.18900.a.B2.GE38817	soluble domain #1		27	283
BupsM.18900.a.B3.GE38818	soluble domain #2		304	489

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|884204.6.peg.5403
RefSeq:	YP_006278021.1
RefSeq:	WP_004524236.1
UniProt:	A0A0H3HUZ0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPLGYMVIAG AVAVAIVMMS ICAAILRDSR DDAFEHAQRA ARNTLQMIER DVTRGFRLYD RTLARIAAQF GDRRIAALPA GLRREILFDS AAAANEAGVM YVLDASGHVT LASTRGPLPA ERFASYDFFA AQRDTPALGM YLSGPYRMSS RDGEPYVALS RRIERADGAF AGVVVLAVRI AYFRQLLAAA NVGPHGALVL MHTDGRILMR IPFDEKFVGL DLTGTSLYSR MQFGRSGEFF DVEPIDHVKR FYLYRRLDDV PLVVQVAIAD DDIYATWRLR AWRFGVLTGV FALIFVVMSA YLAHSLWRKS TAEAALARLA GTDSLTGLYN RRALDETLER EWRRAVGGDR PLAILFVDID HFKRYNDTYG HQVGDEVLAT VSRCIAQQAA RLGDVVARYG GEEFVVVLPD TPRGGALVVA ERVRLGVRML GIEHEGSEVG FVTVSVGVAV WHPDGASAPH IAAIVEAADQ ALYQAKADGR NRVVDLGLG

NT Sequence

atgccgctcg gctatatggt gatcgccggc gcggtggcgg tcgcgatcgt catgatgtcc atctgcgcgg cgatcctgcg cgacagccgc gacgacgcct tcgagcacgc gcagcgcgcc gcgcgcaaca cgctgcagat gatcgagcgc gacgtcacgc gcggctttcg gctgtacgac cgcacgctcg cgcgcatcgc cgcgcagttc ggcgatcgcc ggatcgcggc gctgcccgcc ggtctgcgcc gcgagatcct gttcgattcc gcggcggccg ccaacgaggc cggcgtgatg tacgtgctcg acgcgagcgg gcacgtgacg ctcgcgtcga cgcgcggccc gctgccggcc gagcgcttcg cgtcgtacga ttttttcgcc gcgcagcgcg atacgccggc gctcggcatg tatctgagcg ggccgtaccg gatgtcgtcg cgcgacggcg agccgtacgt cgcgttgtcg cgccgcatcg agcgcgcgga cggcgcgttt gcgggcgtcg tcgtgctcgc ggtgcgcatc gcgtatttcc ggcaactgct ggcggcggcg aatgtgggcc cgcacggcgc gctcgtgctg atgcacacgg acgggcgcat cctgatgcgg attccgttcg acgagaaatt cgtcggcctc gacctgaccg ggacgagcct ctactcgcgc atgcagttcg gccgttccgg cgagttcttc gacgtcgagc cgatcgatca cgtgaagcgc ttctatctgt atcgccggct cgacgacgtt ccgctcgtcg tgcaggtggc gatcgccgac gacgacatct atgcgacctg gcgtctgcgc gcgtggcgct tcggcgtgct gacgggcgtg ttcgcgctga tcttcgtcgt gatgagcgcg tacctcgcgc attcgctgtg gcgcaagtcg acggccgagg cggcgctcgc gcggctcgcc ggcaccgaca gcctcacggg cctctacaac cggcgcgcgc tcgacgagac gctcgagcgc gaatggcggc gcgcggtggg cggcgatcgt ccgctcgcga tcctgttcgt cgacatcgat cacttcaagc gctacaacga cacgtacggc catcaggtcg gcgacgaggt gctcgcgacg gtgtcgcgct gcatcgcgca gcaggcggca cggctcggcg acgtcgtcgc gcgctacggc ggcgaggaat tcgtcgtcgt gctgccggat acgccgcgcg gcggcgcgct cgtcgtcgcc gagcgcgtgc ggctcggcgt gcggatgctc ggcatcgagc acgaaggctc ggaggtcggg ttcgtgacgg tcagcgtcgg cgtcgcggtg tggcatccgg acggcgcgtc cgcgccgcat atcgccgcga tcgtcgaggc ggccgatcag gcgctctatc aggcgaaggc ggacgggcgc aatcgcgtcg tcgacctcgg gctcggctaa

Details for BupsM.18900.a.B2.GE38817

HARVESTED ON:	1/22/2015
SEQUENCED ON:	1/15/2015
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	97
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMR DSRDDAFEHA QRAARNTLQM IERDVTRGFR LYDRTLARIA AQFGDRRIAA LPAGLRREIL FDSAAAANEA GVMYVLDASG HVTLASTRGP LPAERFASYD FFAAQRDTPA LGMYLSGPYR MSSRDGEPYV ALSRRIERAD GAFAGVVVLA VRIAYFRQLL AAANVGPHGA LVLMHTDGRI LMRIPFDEKF VGLDLTGTSL YSRMQFGRSG EFFDVEPIDH VKRFYLYRXX DDVPLVVQVA IADDDIYAXX XLRAXX

Validated NT Sequence

accatggctc accaccacca ccaccatatg cgcgacagcc gcgacgacgc cttcgagcac gcgcagcgcg ccgcgcgcaa cacgctgcag atgatcgagc gcgacgtcac gcgcggcttt cggctgtacg accgcacgct cgcgcgcatc gccgcgcagt tcggcgatcg ccggatcgcg gcgctgcccg ccggtctgcg ccgcgagatc ctgttcgatt ccgcggcggc cgccaacgag gccggcgtga tgtacgtgct cgacgcgagc gggcacgtga cgctcgcgtc gacgcgcggc ccgctgccgg ccgagcgctt cgcgtcgtac gattttttcg ccgcgcagcg cgatacgccg gcgctcggca tgtatctgag cgggccgtac cggatgtcgt cgcgcgacgg cgagccgtac gtcgcgttgt cgcgccgcat cgagcgcgcg gacggcgcgt ttgcgggcgt cgtcgtgctc gcggtgcgca tcgcgtattt ccggcaactg ctggcggcgg cgaatgtggg cccgcacggc gcgctcgtgc tgatgcacac ggacgggcgc atcctgatgc ggattccgtt cgacgagaaa ttcgtcggcc tcgacctgac cgggacgagc ctctactcgc gcatgcagtt cggccgttcc ggcgagttct tcgacgtcga gccgatcgat cacgtgaagc gcttctatct gtatcgccnn nncgacgacg ttccgctcgt cgtgcaggtg gcgatcgccg acgacgacat ctatgcgnnn nnnnnnctgc gcgcgtnnng ctaannnagt aagat

Expected Protein Sequence

MAHHHHHHRD SRDDAFEHAQ RAARNTLQMI ERDVTRGFRL YDRTLARIAA QFGDRRIAAL PAGLRREILF DSAAAANEAG VMYVLDASGH VTLASTRGPL PAERFASYDF FAAQRDTPAL GMYLSGPYRM SSRDGEPYVA LSRRIERADG AFAGVVVLAV RIAYFRQLLA AANVGPHGAL VLMHTDGRIL MRIPFDEKFV GLDLTGTSLY SRMQFGRSGE FFDVEPIDHV KRFYLYRRLD DVPLVVQVAI ADDDIYATWR LRAWR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcgcgaca gccgcgacga cgccttcgag cacgcgcagc gcgccgcgcg caacacgctg cagatgatcg agcgcgacgt cacgcgcggc tttcggctgt acgaccgcac gctcgcgcgc atcgccgcgc agttcggcga tcgccggatc gcggcgctgc ccgccggtct gcgccgcgag atcctgttcg attccgcggc ggccgccaac gaggccggcg tgatgtacgt gctcgacgcg agcgggcacg tgacgctcgc gtcgacgcgc ggcccgctgc cggccgagcg cttcgcgtcg tacgattttt tcgccgcgca gcgcgatacg ccggcgctcg gcatgtatct gagcgggccg taccggatgt cgtcgcgcga cggcgagccg tacgtcgcgt tgtcgcgccg catcgagcgc gcggacggcg cgtttgcggg cgtcgtcgtg ctcgcggtgc gcatcgcgta tttccggcaa ctgctggcgg cggcgaatgt gggcccgcac ggcgcgctcg tgctgatgca cacggacggg cgcatcctga tgcggattcc gttcgacgag aaattcgtcg gcctcgacct gaccgggacg agcctctact cgcgcatgca gttcggccgt tccggcgagt tcttcgacgt cgagccgatc gatcacgtga agcgcttcta tctgtatcgc cggctcgacg acgttccgct cgtcgtgcag gtggcgatcg ccgacgacga catctatgcg acctggcgtc tgcgcgcgtg gcgctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for BupsM.18900.a.B3.GE38818

HARVESTED ON:	1/22/2015
SEQUENCED ON:	1/15/2015
EXPECTED MW:	21kDa
OBSERVED MW:	20kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMH SLWRKSTAEA ALARLAGTDS LTGLYNRRAL DETLEREWRR AVGGDRPLAI LFVDIDHFKR YNDTYGHQVG DEVLATVSRC IAQQAARLGD VVARYGGEEF VVVLPDTPRG GALVVAERVR LGVRMLGIEH EGSEVGFVTV SVGVAVWHPD GASAPHIAAI VEAADQALYQ AKADGRNRVV DLGLG

Validated NT Sequence

atggctcacc accaccacca ccatatgcat tcgctgtggc gcaagtcgac ggccgaggcg gcgctcgcgc ggctcgccgg caccgacagc ctcacgggcc tctacaaccg gcgcgcgctc gacgagacgc tcgagcgcga atggcggcgc gcggtgggcg gcgatcgtcc gctcgcgatc ctgttcgtcg acatcgatca cttcaagcgc tacaacgaca cgtacggcca tcaggtcggc gacgaggtgc tcgcgacggt gtcgcgctgc atcgcgcagc aggcggcacg gctcggcgac gtcgtcgcgc gctacggcgg cgaggaattc gtcgtcgtgc tgccggatac gccgcgcggc ggcgcgctcg tcgtcgccga gcgcgtgcgg ctcggcgtgc ggatgctcgg catcgagcac gaaggctcgg aggtcgggtt cgtgacggtc agcgtcggcg tcgcggtgtg gcatccggac ggcgcgtccg cgccgcatat cgccgcgatc gtcgaggcgg ccgatcaggc gctctatcag gcgaaggcgg acgggcgcaa tcgcgtcgtc gacctcgggc tcggctaagt gagtaagat

Expected Protein Sequence

MAHHHHHHHS LWRKSTAEAA LARLAGTDSL TGLYNRRALD ETLEREWRRA VGGDRPLAIL FVDIDHFKRY NDTYGHQVGD EVLATVSRCI AQQAARLGDV VARYGGEEFV VVLPDTPRGG ALVVAERVRL GVRMLGIEHE GSEVGFVTVS VGVAVWHPDG ASAPHIAAIV EAADQALYQA KADGRNRVVD LGLG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcattcgc tgtggcgcaa gtcgacggcc gaggcggcgc tcgcgcggct cgccggcacc gacagcctca cgggcctcta caaccggcgc gcgctcgacg agacgctcga gcgcgaatgg cggcgcgcgg tgggcggcga tcgtccgctc gcgatcctgt tcgtcgacat cgatcacttc aagcgctaca acgacacgta cggccatcag gtcggcgacg aggtgctcgc gacggtgtcg cgctgcatcg cgcagcaggc ggcacggctc ggcgacgtcg tcgcgcgcta cggcggcgag gaattcgtcg tcgtgctgcc ggatacgccg cgcggcggcg cgctcgtcgt cgccgagcgc gtgcggctcg gcgtgcggat gctcggcatc gagcacgaag gctcggaggt cgggttcgtg acggtcagcg tcggcgtcgc ggtgtggcat ccggacggcg cgtccgcgcc gcatatcgcc gcgatcgtcg aggcggccga tcaggcgctc tatcaggcga aggcggacgg gcgcaatcgc gtcgtcgacc tcgggctcgg ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat