SSGCID: Target Page

BupsM.18138.b

EAL domain-containing protein

CENTER ID:	BupsM.18138.b
ORGANISM:	Burkholderia pseudomallei 1026b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsM.18138.b.B1.GE38816	full length		1	424

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsM.18138.b.B1.PS38079	full length		1	424

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|884204.6.peg.1735
RefSeq:	YP_006275648.1
RefSeq:	WP_004530843.1
UniProt:	A0A0H3HQS1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSMIDIDPPS FQSPRPIAGD DGNRRTVLYG DYTVFSVFQP VFSVSHRRAI GYHASLRAHD SQGNQVPSHE VFTQAARRGD LLELGRLAES LHLGNFHTFD SHDEWLFLSL HPAALMDTVY GDALLANLKA LGLPPQRVVL EVPEQAGGET PRFAAIVDGL RKAGFLIALG GFGAKHSNID RVWHLHPDIV TLDRGILAQA SEHSHLERVL PGLVSLLHES GQLVLMGGIT TEREALIALE CDVDFVQGQY FAGPSVEPVQ PQAAAGVMDT LSAALRLRVA ERTRAQQARL APYVAALLQA SALLREGRDL TEASKELLEL PEAARCFLLD ASGRQIGDNV LPRVHASQRA KRFSPLLHSE GASWERRPYF IEAMRVPGRA HFTPPYLSIN EAHLCVTASI ATPAVQGMQV LCVDINWEAA AHRD

NT Sequence

atgagcatga tcgacatcga tcctcccagc ttccagtcgc cgcgcccgat cgcgggcgac gacggcaacc ggcgcaccgt gctgtacggc gactacaccg tgttcagcgt gttccagccg gtcttctcgg tgtcgcatcg ccgtgcgatc ggctatcacg cgtcgctgcg cgcgcacgat tcgcagggca accaggtgcc ctcgcacgag gtgttcacgc aggccgcgcg ccgcggcgat ctgctcgaac tcggccgcct tgccgaatcg ctgcacctgg gcaacttcca cacgttcgac agtcacgacg aatggctctt cctgagcctg catcccgccg cgctgatgga caccgtctac ggcgacgcgc tcctcgcgaa cctgaaggcg ctcgggctgc cgccgcagcg cgtcgtgctc gaagtgcccg agcaggcggg cggcgagacg ccgcgcttcg cggcgatcgt cgacggcctg cgcaaggcgg gtttcctgat cgcgctcggc ggcttcggcg cgaagcactc gaacatcgat cgcgtctggc acctgcaccc ggacatcgtc acgctcgatc gcggcatcct cgcgcaggca agcgagcatt cgcatctcga gcgcgtgctg cccggcctcg tgtcgctgct gcacgagtcg gggcagctcg tgctgatggg cggcatcacg accgagcgcg aagcgctgat cgcgctcgaa tgcgacgtcg atttcgtaca agggcaatac ttcgccgggc cgagcgtcga gccggtgcag ccgcaggccg cggcgggcgt catggatacg ctgtcggccg cgcttcggct gcgcgtcgcc gagcgcacgc gcgcgcagca agcgcgcctc gcgccgtacg tcgccgcgct gctgcaggcg agcgcgctgc tgcgcgaagg ccgtgacctc accgaagcgt cgaaggagct gctcgagctg cccgaggcgg cgcgctgctt cctgctcgac gcgtcgggcc gtcagatcgg cgacaacgtg ctgccgcgcg tgcatgcatc gcagcgcgcg aagcgcttca gcccgctgct gcactcggaa ggcgcgagct gggaacgccg gccgtacttc atcgaggcga tgcgcgtgcc ggggcgcgcg catttcacgc cgccctatct gtcgatcaat gaagcgcacc tgtgcgtgac ggcgtcgatc gcgacgcccg cggtgcaagg catgcaggtg ctctgcgtcg acatcaactg ggaagccgcc gcgcaccgcg actga

Details for BupsM.18138.b.B1.GE38816

HARVESTED ON:	1/22/2015
SEQUENCED ON:	1/15/2015
EXPECTED MW:	47kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMS MIDIDPPSFQ SPRPIAGDDG NRRTVLYGDY TVFSVFQPVF SVSHRRAIGY HASLRAHDSQ GNQVPSHEVF TQAARRGDLL ELGRLAESLH LGNFHTFDSH DEWLFLSLHP AALMDTVYGD ALLANLKALG LPPQRVVLEV PEQAGGETPR FAAIVDGLRK AGFLIALGGF GAKHSNIDRV WHLHPDIVTL DRGILAQASE HSHLERVLPG LVSLLHESGQ LVLMGGITTE REALIALECD VDFVQGQYFA GPSVEPVQPQ AAAGVMDTLS AALRLRVAER TRAQQARLAP YVAALLQASA LLREGRDLTE ASKELLELPE AARCFLLDAS GRQIGDNVLP RVHASQRAKR FSPLLHSEGA SWERRPYFIE AMRVPGRAHF TPPYLSINEA HLCVTASIAT PAVQGMQVLC VDINW

Validated NT Sequence

atggctcacc accaccacca ccatatgagc atgatcgaca tcgatcctcc cagcttccag tcgccgcgcc cgatcgcggg cgacgacggc aaccggcgca ccgtgctgta cggcgactac accgtgttca gcgtgttcca gccggtcttc tcggtgtcgc atcgccgtgc gatcggctat cacgcgtcgc tgcgcgcgca cgattcgcag ggcaaccagg tgccctcgca cgaggtgttc acgcaggccg cgcgccgcgg cgatctgctc gaactcggcc gccttgccga atcgctgcac ctgggcaact tccacacgtt cgacagtcac gacgaatggc tcttcctgag cctgcatccc gccgcgctga tggacaccgt ctacggcgac gcgctcctcg cgaacctgaa ggcgctcggg ctgccgccgc agcgcgtcgt gctcgaagtg cccgagcagg cgggcggcga gacgccgcgc ttcgcggcga tcgtcgacgg cctgcgcaag gcgggtttcc tgatcgcgct cggcggcttc ggcgcgaagc actcgaacat cgatcgcgtc tggcacctgc acccggacat cgtcacgctc gatcgcggca tcctcgcgca ggcaagcgag cattcgcatc tcgagcgcgt gctgcccggc ctcgtgtcgc tgctgcacga gtcggggcag ctcgtgctga tgggcggcat cacgaccgag cgcgaagcgc tgatcgcgct cgaatgcgac gtcgatttcg tacaagggca atacttcgcc gggccgagcg tcgagccggt gcagccgcag gccgcggcgg gcgtcatgga tacgctgtcg gccgcgcttc ggctgcgcgt cgccgagcgc acgcgcgcgc agcaagcgcg cctcgcgccg tacgtcgccg cgctgctgca ggcgagcgcg ctgctgcgcg aaggccgtga cctcaccgaa gcgtcgaagg agctgctcga gctgcccgag gcggcgcgct gcttcctgct cgacgcgtcg ggccgtcaga tcggcgacaa cgtgctgccg cgcgtgcatg catcgcagcg cgcgaagcgc ttcagcccgc tgctgcactc ggaaggcgcg agctgggaac gccggccgta cttcatcgag gcgatgcgcg tgccggggcg cgcgcatttc acgccgccct atctgtcgat caatgaagcg cacctgtgcg tgacggcgtc gatcgcgacg cccgcggtgc aaggcatgca ggtgctctgc gtcgacatca actggga

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca tgatcgacat cgatcctccc agcttccagt cgccgcgccc gatcgcgggc gacgacggca accggcgcac cgtgctgtac ggcgactaca ccgtgttcag cgtgttccag ccggtcttct cggtgtcgca tcgccgtgcg atcggctatc acgcgtcgct gcgcgcgcac gattcgcagg gcaaccaggt gccctcgcac gaggtgttca cgcaggccgc gcgccgcggc gatctgctcg aactcggccg ccttgccgaa tcgctgcacc tgggcaactt ccacacgttc gacagtcacg acgaatggct cttcctgagc ctgcatcccg ccgcgctgat ggacaccgtc tacggcgacg cgctcctcgc gaacctgaag gcgctcgggc tgccgccgca gcgcgtcgtg ctcgaagtgc ccgagcaggc gggcggcgag acgccgcgct tcgcggcgat cgtcgacggc ctgcgcaagg cgggtttcct gatcgcgctc ggcggcttcg gcgcgaagca ctcgaacatc gatcgcgtct ggcacctgca cccggacatc gtcacgctcg atcgcggcat cctcgcgcag gcaagcgagc attcgcatct cgagcgcgtg ctgcccggcc tcgtgtcgct gctgcacgag tcggggcagc tcgtgctgat gggcggcatc acgaccgagc gcgaagcgct gatcgcgctc gaatgcgacg tcgatttcgt acaagggcaa tacttcgccg ggccgagcgt cgagccggtg cagccgcagg ccgcggcggg cgtcatggat acgctgtcgg ccgcgcttcg gctgcgcgtc gccgagcgca cgcgcgcgca gcaagcgcgc ctcgcgccgt acgtcgccgc gctgctgcag gcgagcgcgc tgctgcgcga aggccgtgac ctcaccgaag cgtcgaagga gctgctcgag ctgcccgagg cggcgcgctg cttcctgctc gacgcgtcgg gccgtcagat cggcgacaac gtgctgccgc gcgtgcatgc atcgcagcgc gcgaagcgct tcagcccgct gctgcactcg gaaggcgcga gctgggaacg ccggccgtac ttcatcgagg cgatgcgcgt gccggggcgc gcgcatttca cgccgcccta tctgtcgatc aatgaagcgc acctgtgcgt gacggcgtcg atcgcgacgc ccgcggtgca aggcatgcag gtgctctgcg tcgacatcaa ctgggaagcc gccgcgcacc gcgactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for BupsM.18138.b.B1.PS38079

PURIFICATION DATe:	10/13/2016
CONCENTRATION:	25.35mg/ml
OBSERVED MW:	38kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	SEC
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	14
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCA TGATCGACAT CGATCCTCCC AGCTTCCAGT CGCCGCGCCC GATCGCGGGC GACGACGGCA ACCGGCGCAC CGTGCTGTAC GGCGACTACA CCGTGTTCAG CGTGTTCCAG CCGGTCTTCT CGGTGTCGCA TCGCCGTGCG ATCGGCTATC ACGCGTCGCT GCGCGCGCAC GATTCGCAGG GCAACCAGGT GCCCTCGCAC GAGGTGTTCA CGCAGGCCGC GCGCCGCGGC GATCTGCTCG AACTCGGCCG CCTTGCCGAA TCGCTGCACC TGGGCAACTT CCACACGTTC GACAGTCACG ACGAATGGCT CTTCCTGAGC CTGCATCCCG CCGCGCTGAT GGACACCGTC TACGGCGACG CGCTCCTCGC GAACCTGAAG GCGCTCGGGC TGCCGCCGCA GCGCGTCGTG CTCGAAGTGC CCGAGCAGGC GGGCGGCGAG ACGCCGCGCT TCGCGGCGAT CGTCGACGGC CTGCGCAAGG CGGGTTTCCT GATCGCGCTC GGCGGCTTCG GCGCGAAGCA CTCGAACATC GATCGCGTCT GGCACCTGCA CCCGGACATC GTCACGCTCG ATCGCGGCAT CCTCGCGCAG GCAAGCGAGC ATTCGCATCT CGAGCGCGTG CTGCCCGGCC TCGTGTCGCT GCTGCACGAG TCGGGGCAGC TCGTGCTGAT GGGCGGCATC ACGACCGAGC GCGAAGCGCT GATCGCGCTC GAATGCGACG TCGATTTCGT ACAAGGGCAA TACTTCGCCG GGCCGAGCGT CGAGCCGGTG CAGCCGCAGG CCGCGGCGGG CGTCATGGAT ACGCTGTCGG CCGCGCTTCG GCTGCGCGTC GCCGAGCGCA CGCGCGCGCA GCAAGCGCGC CTCGCGCCGT ACGTCGCCGC GCTGCTGCAG GCGAGCGCGC TGCTGCGCGA AGGCCGTGAC CTCACCGAAG CGTCGAAGGA GCTGCTCGAG CTGCCCGAGG CGGCGCGCTG CTTCCTGCTC GACGCGTCGG GCCGTCAGAT CGGCGACAAC GTGCTGCCGC GCGTGCATGC ATCGCAGCGC GCGAAGCGCT TCAGCCCGCT GCTGCACTCG GAAGGCGCGA GCTGGGAACG CCGGCCGTAC TTCATCGAGG CGATGCGCGT GCCGGGGCGC GCGCATTTCA CGCCGCCCTA TCTGTCGATC AATGAAGCGC ACCTGTGCGT GACGGCGTCG ATCGCGACGC CCGCGGTGCA AGGCATGCAG GTGCTCTGCG TCGACATCAA CTGGGAAGCC GCCGCGCACC GCGACTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT