BupsM.18138.a

EAL domain-containing protein

CENTER ID: BupsM.18138.a
ORGANISM: Burkholderia pseudomallei 1026b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsM.18138.a.B1.GE38815 full length 1 427
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsM.18138.a.B1.PS02422 full length 1 427

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|884204.6.peg.2173
RefSeq: YP_006275265.1
RefSeq: WP_004553987.1
UniProt: A0A0H3HLP7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPKTQGFSPE SLISQFTRCD AGWCVRYHDV TLASAFQPVI SITHKRVVGY EALARATDAN GAPISPDTLF ARAQARGETI LLDRLTRCLH VANFAAQDTG ACWLFLNVLP QMFDAGIAPG AFIEALCAHF ALPPTRVVLE VIEQPSRNEV ALARTIDMIQ HGDFLIAVDD FGTGFSNFDR IWQIKPDIVK LDRSILERSL AASDAHRIIH HLVTMLHHAG TMVLAEGVEN EDALQILMDA DVDFVQGFCF GRPAPSLEHA RRAAPARIEA AWRRFAERTK ERRGDVVHPG FDTIERIMLT GAADYAQTRN LRDAAQRLLT SPIVRRVFVA EANGEQVEPS VTIETPDAPS ATARRLAPLL PELHCNWSRR AYFQRAIAAP GRVALMGPHF SLTDGRDCYT AAVAIHLGTT LKVFCVDFDF SSYGDGR
NT Sequence
atgccgaaaa cgcaagggtt ctcgcccgaa agcctgattt cgcagttcac ccgctgcgac gcaggctggt gtgtgcgata tcacgacgtc acccttgcca gcgcctttca gccggtcatc tcgatcacgc acaagcgcgt cgtcggctat gaggcgctcg cccgcgcgac cgacgcgaac ggcgcgccga tttcgcccga cacgctgttc gcgcgtgcgc aggcgcgcgg cgagacgatc ctgctcgatc ggctgacccg gtgcctgcac gtcgcgaact tcgccgcgca ggacacgggc gcgtgctggc tgttcctgaa cgtgctgccg cagatgttcg acgccggcat cgcgccgggc gcgttcatcg aggcgctttg cgcgcacttc gcgctgccgc cgacgcgcgt cgtgctcgaa gtgatcgagc agccgtcgcg caacgaggtc gcgctcgccc gcacgatcga catgatccag cacggcgact tcctgatcgc ggtcgacgat ttcggcaccg gcttctcgaa cttcgaccgc atctggcaga tcaagcccga catcgtgaag ctcgaccgct cgatcctcga gcgctcgctc gcggcaagcg acgcgcatcg gatcattcat catctcgtca cgatgctgca tcacgcgggc acgatggtgc tcgcggaggg cgtcgaaaac gaagacgcgc tgcagatcct gatggacgcc gacgtcgatt tcgtgcaggg cttctgcttc ggccggcccg ccccgtcgct cgagcacgcg cgccgcgccg cgccggcgcg catcgaggcc gcgtggcggc gcttcgccga acgcacgaag gaacggcgtg gcgacgtcgt gcatcccggc ttcgatacga tcgagcggat catgctcacg ggcgccgccg actatgcgca gacccgaaac ctgcgcgacg ccgcgcagcg gctgctgacg agcccgatcg tgcggcgcgt gttcgtggcg gaagcgaacg gcgagcaagt ggagccatcg gtcacgatcg agacgccgga cgcgccgagc gcgacggcgc ggcggctcgc gccgctgctg cccgagctgc actgcaactg gtcgcggcgc gcgtacttcc agcgcgcgat cgcggcgccc ggacgcgtcg cgctgatggg cccgcacttt tcgctgacgg acggccgcga ctgctacacg gccgccgtcg cgatccacct cgggacgacg ctgaaggtgt tctgcgtcga cttcgacttt tcgtcgtacg gggacggacg ctag
Details for BupsM.18138.a.B1.GE38815
HARVESTED ON: 1/22/2015
SEQUENCED ON: 1/15/2015
EXPECTED MW: 48kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 94
PERCENT COVERAGE: 66
Validated AA Sequence
MAHHHHHHMP KTQGFSPESL ISQFTRCDAG WCVRYHDVTL ASAFQPVISI THKRVVGYEA LARATDANGA PISPDTLFAR AQARGETILL DRLTRCLHVA NFAAQDTGAC WLFLNVLPQM FDAGIAPGAF IEALCAHFAL PPTRVVLEVI EQPSRNEVAL ARTIDMIQHG DFLIAVDDFG TGFSNFDRIW QIKPDIVKLD RSILERSLAA SDAHRIIHHL VTMLHHAGTM VLAEGVENED ALQILMDADV DFVQGFCFXR PAPXLEHARR AAXXRIEAXX XRXXXXXXXX
Validated NT Sequence
atggctcacc accaccacca ccatatgccg aaaacgcaag ggttctcgcc cgaaagcctg atttcgcagt tcacccgctg cgacgcaggc tggtgtgtgc gatatcacga cgtcaccctt gccagcgcct ttcagccggt catctcgatc acgcacaagc gcgtcgtcgg ctatgaggcg ctcgcccgcg cgaccgacgc gaacggcgcg ccgatttcgc ccgacacgct gttcgcgcgt gcgcaggcgc gcggcgagac gatcctgctc gatcggctga cccggtgcct gcacgtcgcg aacttcgccg cgcaggacac gggcgcgtgc tggctgttcc tgaacgtgct gccgcagatg ttcgacgccg gcatcgcgcc gggcgcgttc atcgaggcgc tttgcgcgca cttcgcgctg ccgccgacgc gcgtcgtgct cgaagtgatc gagcagccgt cgcgcaacga ggtcgcgctc gcccgcacga tcgacatgat ccagcacggc gacttcctga tcgcggtcga cgatttcggc accggcttct cgaacttcga ccgcatctgg cagatcaagc ccgacatcgt gaagctcgac cgctcgatcc tcgagcgctc gctcgcggca agcgacgcgc atcggatcat tcatcatctc gtcacgatgc tgcatcacgc gggcacgatg gtgctcgcgg agggcgtcga aaacgaagac gcgctgcaga tcctgatgga cgccgacgtc gatttcgtgc agggcttctg cttcnnccgg cccgccccgt nnctcgagca cgcgcgccgc gccgcgnnnn cgcgcatcga ggcnncgtng cnncgctnnn nnnnnnnnan gnnnnaacnn c
Expected Protein Sequence
MAHHHHHHMP KTQGFSPESL ISQFTRCDAG WCVRYHDVTL ASAFQPVISI THKRVVGYEA LARATDANGA PISPDTLFAR AQARGETILL DRLTRCLHVA NFAAQDTGAC WLFLNVLPQM FDAGIAPGAF IEALCAHFAL PPTRVVLEVI EQPSRNEVAL ARTIDMIQHG DFLIAVDDFG TGFSNFDRIW QIKPDIVKLD RSILERSLAA SDAHRIIHHL VTMLHHAGTM VLAEGVENED ALQILMDADV DFVQGFCFGR PAPSLEHARR AAPARIEAAW RRFAERTKER RGDVVHPGFD TIERIMLTGA ADYAQTRNLR DAAQRLLTSP IVRRVFVAEA NGEQVEPSVT IETPDAPSAT ARRLAPLLPE LHCNWSRRAY FQRAIAAPGR VALMGPHFSL TDGRDCYTAA VAIHLGTTLK VFCVDFDFSS YGDGR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccga aaacgcaagg gttctcgccc gaaagcctga tttcgcagtt cacccgctgc gacgcaggct ggtgtgtgcg atatcacgac gtcacccttg ccagcgcctt tcagccggtc atctcgatca cgcacaagcg cgtcgtcggc tatgaggcgc tcgcccgcgc gaccgacgcg aacggcgcgc cgatttcgcc cgacacgctg ttcgcgcgtg cgcaggcgcg cggcgagacg atcctgctcg atcggctgac ccggtgcctg cacgtcgcga acttcgccgc gcaggacacg ggcgcgtgct ggctgttcct gaacgtgctg ccgcagatgt tcgacgccgg catcgcgccg ggcgcgttca tcgaggcgct ttgcgcgcac ttcgcgctgc cgccgacgcg cgtcgtgctc gaagtgatcg agcagccgtc gcgcaacgag gtcgcgctcg cccgcacgat cgacatgatc cagcacggcg acttcctgat cgcggtcgac gatttcggca ccggcttctc gaacttcgac cgcatctggc agatcaagcc cgacatcgtg aagctcgacc gctcgatcct cgagcgctcg ctcgcggcaa gcgacgcgca tcggatcatt catcatctcg tcacgatgct gcatcacgcg ggcacgatgg tgctcgcgga gggcgtcgaa aacgaagacg cgctgcagat cctgatggac gccgacgtcg atttcgtgca gggcttctgc ttcggccggc ccgccccgtc gctcgagcac gcgcgccgcg ccgcgccggc gcgcatcgag gccgcgtggc ggcgcttcgc cgaacgcacg aaggaacggc gtggcgacgt cgtgcatccc ggcttcgata cgatcgagcg gatcatgctc acgggcgccg ccgactatgc gcagacccga aacctgcgcg acgccgcgca gcggctgctg acgagcccga tcgtgcggcg cgtgttcgtg gcggaagcga acggcgagca agtggagcca tcggtcacga tcgagacgcc ggacgcgccg agcgcgacgg cgcggcggct cgcgccgctg ctgcccgagc tgcactgcaa ctggtcgcgg cgcgcgtact tccagcgcgc gatcgcggcg cccggacgcg tcgcgctgat gggcccgcac ttttcgctga cggacggccg cgactgctac acggccgccg tcgcgatcca cctcgggacg acgctgaagg tgttctgcgt cgacttcgac ttttcgtcgt acggggacgg acgctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BupsM.18138.a.B1.PS02422
PURIFICATION DATe: 6/18/2015
CONCENTRATION: 25.66mg/ml
OBSERVED MW: 49kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 9
VIAL VOLUME: 110µl
PERCENT IDENTITY: 94
PERCENT COVERAGE: 66
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMP KTQGFSPESL ISQFTRCDAG WCVRYHDVTL ASAFQPVISI THKRVVGYEA LARATDANGA PISPDTLFAR AQARGETILL DRLTRCLHVA NFAAQDTGAC WLFLNVLPQM FDAGIAPGAF IEALCAHFAL PPTRVVLEVI EQPSRNEVAL ARTIDMIQHG DFLIAVDDFG TGFSNFDRIW QIKPDIVKLD RSILERSLAA SDAHRIIHHL VTMLHHAGTM VLAEGVENED ALQILMDADV DFVQGFCFXR PAPXLEHARR AAXXRIEAXX XRXXXXXXXX
Validated NT Sequence
atggctcacc accaccacca ccatatgccg aaaacgcaag ggttctcgcc cgaaagcctg atttcgcagt tcacccgctg cgacgcaggc tggtgtgtgc gatatcacga cgtcaccctt gccagcgcct ttcagccggt catctcgatc acgcacaagc gcgtcgtcgg ctatgaggcg ctcgcccgcg cgaccgacgc gaacggcgcg ccgatttcgc ccgacacgct gttcgcgcgt gcgcaggcgc gcggcgagac gatcctgctc gatcggctga cccggtgcct gcacgtcgcg aacttcgccg cgcaggacac gggcgcgtgc tggctgttcc tgaacgtgct gccgcagatg ttcgacgccg gcatcgcgcc gggcgcgttc atcgaggcgc tttgcgcgca cttcgcgctg ccgccgacgc gcgtcgtgct cgaagtgatc gagcagccgt cgcgcaacga ggtcgcgctc gcccgcacga tcgacatgat ccagcacggc gacttcctga tcgcggtcga cgatttcggc accggcttct cgaacttcga ccgcatctgg cagatcaagc ccgacatcgt gaagctcgac cgctcgatcc tcgagcgctc gctcgcggca agcgacgcgc atcggatcat tcatcatctc gtcacgatgc tgcatcacgc gggcacgatg gtgctcgcgg agggcgtcga aaacgaagac gcgctgcaga tcctgatgga cgccgacgtc gatttcgtgc agggcttctg cttcnnccgg cccgccccgt nnctcgagca cgcgcgccgc gccgcgnnnn cgcgcatcga ggcnncgtng cnncgctnnn nnnnnnnnan gnnnnaacnn c
Expressed Protein Sequence
MAHHHHHHMP KTQGFSPESL ISQFTRCDAG WCVRYHDVTL ASAFQPVISI THKRVVGYEA LARATDANGA PISPDTLFAR AQARGETILL DRLTRCLHVA NFAAQDTGAC WLFLNVLPQM FDAGIAPGAF IEALCAHFAL PPTRVVLEVI EQPSRNEVAL ARTIDMIQHG DFLIAVDDFG TGFSNFDRIW QIKPDIVKLD RSILERSLAA SDAHRIIHHL VTMLHHAGTM VLAEGVENED ALQILMDADV DFVQGFCFGR PAPSLEHARR AAPARIEAAW RRFAERTKER RGDVVHPGFD TIERIMLTGA ADYAQTRNLR DAAQRLLTSP IVRRVFVAEA NGEQVEPSVT IETPDAPSAT ARRLAPLLPE LHCNWSRRAY FQRAIAAPGR VALMGPHFSL TDGRDCYTAA VAIHLGTTLK VFCVDFDFSS YGDGR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCGA AAACGCAAGG GTTCTCGCCC GAAAGCCTGA TTTCGCAGTT CACCCGCTGC GACGCAGGCT GGTGTGTGCG ATATCACGAC GTCACCCTTG CCAGCGCCTT TCAGCCGGTC ATCTCGATCA CGCACAAGCG CGTCGTCGGC TATGAGGCGC TCGCCCGCGC GACCGACGCG AACGGCGCGC CGATTTCGCC CGACACGCTG TTCGCGCGTG CGCAGGCGCG CGGCGAGACG ATCCTGCTCG ATCGGCTGAC CCGGTGCCTG CACGTCGCGA ACTTCGCCGC GCAGGACACG GGCGCGTGCT GGCTGTTCCT GAACGTGCTG CCGCAGATGT TCGACGCCGG CATCGCGCCG GGCGCGTTCA TCGAGGCGCT TTGCGCGCAC TTCGCGCTGC CGCCGACGCG CGTCGTGCTC GAAGTGATCG AGCAGCCGTC GCGCAACGAG GTCGCGCTCG CCCGCACGAT CGACATGATC CAGCACGGCG ACTTCCTGAT CGCGGTCGAC GATTTCGGCA CCGGCTTCTC GAACTTCGAC CGCATCTGGC AGATCAAGCC CGACATCGTG AAGCTCGACC GCTCGATCCT CGAGCGCTCG CTCGCGGCAA GCGACGCGCA TCGGATCATT CATCATCTCG TCACGATGCT GCATCACGCG GGCACGATGG TGCTCGCGGA GGGCGTCGAA AACGAAGACG CGCTGCAGAT CCTGATGGAC GCCGACGTCG ATTTCGTGCA GGGCTTCTGC TTCGGCCGGC CCGCCCCGTC GCTCGAGCAC GCGCGCCGCG CCGCGCCGGC GCGCATCGAG GCCGCGTGGC GGCGCTTCGC CGAACGCACG AAGGAACGGC GTGGCGACGT CGTGCATCCC GGCTTCGATA CGATCGAGCG GATCATGCTC ACGGGCGCCG CCGACTATGC GCAGACCCGA AACCTGCGCG ACGCCGCGCA GCGGCTGCTG ACGAGCCCGA TCGTGCGGCG CGTGTTCGTG GCGGAAGCGA ACGGCGAGCA AGTGGAGCCA TCGGTCACGA TCGAGACGCC GGACGCGCCG AGCGCGACGG CGCGGCGGCT CGCGCCGCTG CTGCCCGAGC TGCACTGCAA CTGGTCGCGG CGCGCGTACT TCCAGCGCGC GATCGCGGCG CCCGGACGCG TCGCGCTGAT GGGCCCGCAC TTTTCGCTGA CGGACGGCCG CGACTGCTAC ACGGCCGCCG TCGCGATCCA CCTCGGGACG ACGCTGAAGG TGTTCTGCGT CGACTTCGAC TTTTCGTCGT ACGGGGACGG ACGCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT