SSGCID: Target Page

BupsM.17371.a

ABC transporter, periplasmic substrate-binding protein (BP1026B_II0374)

CENTER ID:	BupsM.17371.a
ORGANISM:	Burkholderia pseudomallei 1026b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsM.17371.a.B2.GE42240	mature protein		35	360

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsM.17371.a.B2.PW38475	mature protein		35	360

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|884204.3.peg.4532
UniProt:	A0A0H3HRW6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTYTNFPRGG AWRRFALAAG AAALLQGAAA QAQAAAVVLY TADGLENLYR DVLPAFEKQE GVKVNIVTAG SGEVVNRANV EKGSPKADVI VTLPPFIQQA GQFGLLQPYR SVNYKNVPAI AKAEDGSWAT FVNNYFSFAI NPSVVKSQPK TFADLLHPDY SGKLAYSNPA MAGDGMAVII LTSALMGEDK AFDYLAKLER SVKFHTKGTG YLNVLLSRNE IAVANGDLQM DLDDAEHGGL SIKPIFVAAK AGEPPTTFQL PYAIGLVKGG PNQDAGRKLI DYLMSADVQA KVPDMFGIPG RTDVPLSGKN GEAVKRAIAG VKLIPVDWDA VMAKKPVWTE RWKKEVIGDS GKQTEVVKPK

NT Sequence

atgacgtata cgaatttccc gcgcggcggc gcctggcgcc gcttcgcgct cgccgccggc gccgccgcgc tgttgcaagg cgcggcagcg caggcgcagg cggcagccgt cgtgctgtac acggcggacg gcctcgagaa cctgtaccgc gacgtgctgc ccgcgttcga gaagcaggaa ggcgtgaagg tgaacatcgt gacggcgggc agcggcgaag tggtgaaccg cgcgaacgtc gagaagggct cgccgaaggc cgacgtgatc gtcacgctgc cgccgttcat tcagcaggcc ggccagttcg gcctgctgca gccgtaccgc agcgtcaact acaagaacgt gccggcgatc gcgaaggcgg aagacggctc atgggcgacg ttcgtcaaca actacttctc gttcgcgatc aacccgtcgg tcgtgaagag ccagccgaag acgttcgccg atctgctgca tcccgattac agcggcaagc tcgcgtattc gaacccggcg atggcgggcg acgggatggc cgtcatcatc ctgacgagcg cgctgatggg cgaggacaag gcgttcgact atctcgcgaa gctcgagcgc agcgtgaagt tccacacgaa gggcacgggc tacctgaacg tgctgctgtc gcgcaacgag atcgcggtcg cgaacggcga tctgcagatg gatctggacg acgccgagca cggcggcctg tcgatcaagc cgatcttcgt cgccgcgaag gcgggcgagc cgccgacgac gttccagctg ccgtacgcga tcggcctcgt caagggcggc ccgaaccagg acgcgggcag gaagctgatc gactacctga tgtcggccga cgtgcaggcg aaggtgcccg acatgttcgg cattccgggc cgcaccgacg tgccgctttc gggcaagaac ggcgaggcgg tgaagcgcgc gatcgccggc gtgaagctga ttccggtcga ctgggacgcg gtgatggcga agaagcccgt gtggaccgag cgctggaaga aggaagtgat cggcgattcg ggcaagcaga ccgaagtcgt caagccgaaa tga

Details for BupsM.17371.a.B2.GE42240

HARVESTED ON:	2/23/2018
SEQUENCED ON:	3/1/2018
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMA AVVLYTADGL ENLYRDVLPA FEKQEGVKVN IVTAGSGEVV NRANVEKGSP KADVIVTLPP FIQQAGQFGL LQPYRSVNYK NVPAIAKAED GSWATFVNNY FSFAINPSVV KSQPKTFADL LHPDYSGKLA YSNPAMAGDG MAVIILTSAL MGEDKAFDYL AKLERSVKFH TKGTGYLNVL LSRNEIAVAN GDLQMDLDDA EHGGLSIKPI FVAAKAGEPP TTFQLPYAIG LVKGGPNQDA GRKLIDYLMS ADVQAKVPDM FGIPGRTDVP LSGKNGEAVK RAIAGVKLIP VDWDAVMAKK PVWTERWKKE VIGDS

Validated NT Sequence

cccgaatcgc cgatcacttc cttcttccag cgctcggtcc acacgggctt cttcgccatc accgcgtccc agtcgaccgg aatcagcttc acgccggcga tcgcgcgctt caccgcctcg ccgttcttgc ccgaaagcgg cacgtcggtg cggcccggaa tgccgaacat gtcgggcacc ttcgcctgca cgtcggccga catcaggtag tcgatcagct tcctgcccgc gtcctggttc gggccgccct tgacgaggcc gatcgcgtac ggcagctgga acgtcgtcgg cggctcgccc gccttcgcgg cgacgaagat cggcttgatc gacaggccgc cgtgctcggc gtcgtccaga tccatctgca gatcgccgtt cgcgaccgcg atctcgttgc gcgacagcag cacgttcagg tagcccgtgc ccttcgtgtg gaacttcacg ctgcgctcga gcttcgcgag atagtcgaac gccttgtcct cgcccatcag cgcgctcgtc aggatgatga cggccatccc gtcgcccgcc atcgccgggt tcgaatacgc gagcttgccg ctgtaatcgg gatgcagcag atcggcgaac gtcttcggct ggctcttcac gaccgacggg ttgatcgcga acgagaagta gttgttgacg aacgtcgccc atgagccgtc ttccgccttc gcgatcgccg gcacgttctt gtagttgacg ctgcggtacg gctgcagcag gccgaactgg ccggcctgct gaatgaacgg cggcagcgtg acgatcacgt cggccttcgg cgagcccttc tcgacgttcg cgcggttcac cacttcgccg ctgcccgccg tcacgatgtt caccttcacg ccttcctgct tctcgaacgc gggcagcacg tcgcggtaca ggttctcgag gccgtccgcc gtgtacagca cgacggctgc catatggtgg tggtggtggt gagccat

Expected Protein Sequence

MAHHHHHHAA VVLYTADGLE NLYRDVLPAF EKQEGVKVNI VTAGSGEVVN RANVEKGSPK ADVIVTLPPF IQQAGQFGLL QPYRSVNYKN VPAIAKAEDG SWATFVNNYF SFAINPSVVK SQPKTFADLL HPDYSGKLAY SNPAMAGDGM AVIILTSALM GEDKAFDYLA KLERSVKFHT KGTGYLNVLL SRNEIAVANG DLQMDLDDAE HGGLSIKPIF VAAKAGEPPT TFQLPYAIGL VKGGPNQDAG RKLIDYLMSA DVQAKVPDMF GIPGRTDVPL SGKNGEAVKR AIAGVKLIPV DWDAVMAKKP VWTERWKKEV IGDSGKQTEV VKPK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcagccg tcgtgctgta cacggcggac ggcctcgaga acctgtaccg cgacgtgctg cccgcgttcg agaagcagga aggcgtgaag gtgaacatcg tgacggcggg cagcggcgaa gtggtgaacc gcgcgaacgt cgagaagggc tcgccgaagg ccgacgtgat cgtcacgctg ccgccgttca ttcagcaggc cggccagttc ggcctgctgc agccgtaccg cagcgtcaac tacaagaacg tgccggcgat cgcgaaggcg gaagacggct catgggcgac gttcgtcaac aactacttct cgttcgcgat caacccgtcg gtcgtgaaga gccagccgaa gacgttcgcc gatctgctgc atcccgatta cagcggcaag ctcgcgtatt cgaacccggc gatggcgggc gacgggatgg ccgtcatcat cctgacgagc gcgctgatgg gcgaggacaa ggcgttcgac tatctcgcga agctcgagcg cagcgtgaag ttccacacga agggcacggg ctacctgaac gtgctgctgt cgcgcaacga gatcgcggtc gcgaacggcg atctgcagat ggatctggac gacgccgagc acggcggcct gtcgatcaag ccgatcttcg tcgccgcgaa ggcgggcgag ccgccgacga cgttccagct gccgtacgcg atcggcctcg tcaagggcgg cccgaaccag gacgcgggca ggaagctgat cgactacctg atgtcggccg acgtgcaggc gaaggtgccc gacatgttcg gcattccggg ccgcaccgac gtgccgcttt cgggcaagaa cggcgaggcg gtgaagcgcg cgatcgccgg cgtgaagctg attccggtcg actgggacgc ggtgatggcg aagaagcccg tgtggaccga gcgctggaag aaggaagtga tcggcgattc gggcaagcag accgaagtcg tcaagccgaa atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for BupsM.17371.a.B2.PW38475

PURIFICATION DATe:	5/14/2018
CONCENTRATION:	24.06mg/ml
OBSERVED MW:	36kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	1
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCAGCCG TCGTGCTGTA CACGGCGGAC GGCCTCGAGA ACCTGTACCG CGACGTGCTG CCCGCGTTCG AGAAGCAGGA AGGCGTGAAG GTGAACATCG TGACGGCGGG CAGCGGCGAA GTGGTGAACC GCGCGAACGT CGAGAAGGGC TCGCCGAAGG CCGACGTGAT CGTCACGCTG CCGCCGTTCA TTCAGCAGGC CGGCCAGTTC GGCCTGCTGC AGCCGTACCG CAGCGTCAAC TACAAGAACG TGCCGGCGAT CGCGAAGGCG GAAGACGGCT CATGGGCGAC GTTCGTCAAC AACTACTTCT CGTTCGCGAT CAACCCGTCG GTCGTGAAGA GCCAGCCGAA GACGTTCGCC GATCTGCTGC ATCCCGATTA CAGCGGCAAG CTCGCGTATT CGAACCCGGC GATGGCGGGC GACGGGATGG CCGTCATCAT CCTGACGAGC GCGCTGATGG GCGAGGACAA GGCGTTCGAC TATCTCGCGA AGCTCGAGCG CAGCGTGAAG TTCCACACGA AGGGCACGGG CTACCTGAAC GTGCTGCTGT CGCGCAACGA GATCGCGGTC GCGAACGGCG ATCTGCAGAT GGATCTGGAC GACGCCGAGC ACGGCGGCCT GTCGATCAAG CCGATCTTCG TCGCCGCGAA GGCGGGCGAG CCGCCGACGA CGTTCCAGCT GCCGTACGCG ATCGGCCTCG TCAAGGGCGG CCCGAACCAG GACGCGGGCA GGAAGCTGAT CGACTACCTG ATGTCGGCCG ACGTGCAGGC GAAGGTGCCC GACATGTTCG GCATTCCGGG CCGCACCGAC GTGCCGCTTT CGGGCAAGAA CGGCGAGGCG GTGAAGCGCG CGATCGCCGG CGTGAAGCTG ATTCCGGTCG ACTGGGACGC GGTGATGGCG AAGAAGCCCG TGTGGACCGA GCGCTGGAAG AAGGAAGTGA TCGGCGATTC GGGCAAGCAG ACCGAAGTCG TCAAGCCGAA ATGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT