SSGCID: Target Page

BupsM.17236.g

Uncharacterized protein

CENTER ID:	BupsM.17236.g
ORGANISM:	Burkholderia pseudomallei 1026b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsM.17236.g.B2.GE38813	soluble domain #1		48	298
BupsM.17236.g.B3.GE38814	soluble domain #2		323	786

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsM.17236.g.B3.PS38089	soluble domain #2		323	786

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|884204.6.peg.1949
RefSeq:	YP_006275455.1
RefSeq:	WP_004185903.1
UniProt:	A0A0H3HM69

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSSSRSHAAR AARPVRATRK SRRRRALFAI PLLGTLALAL LWVAIIARLS VEKDSAFRDA GASAAILSTA LEQHTVKAIH QVDQITRFVK YEFEKSPTRF NLASTVEKGV VPSDTLIQVS ILDARGRLVA NTAEAHPKPI DLSDREHFKV HMQRNDDDLY ISKPVFGRVS RHWTLQMTRR LNHPDGSFAG VVVVSEDPAY FTNDFYNNAT LGKDGVIAVI SDTGAVLARS TGSTQKTPGT FSASGVYPVS ERASGTYVDP IDGIVRIVSY RHLDGYPLGV MVGLSQAEEF ADYHHTRNVY LLMTGFITLA MLAFFGVATG LIGKLLGRER EMTQLAEFDL LTGLANRYAT LRSLRNDVAL PSNLSRLAIL FIDLDNFKTV NDTLGHNAGD IVLQMTAARL SDAVANEGAL ARIGGDEFVV VVKGENVERR AERLAEAIVR MFAEPFDVRG SSFVLHASIG IALHSVENES EIDLLKKADL AMYSAKDAGK NCYQFYAPHL SHRADHLMRW EQQLRVALSD GQLFLAYQPK IDLTYRCITG FEALVRWDHP EHGVISANEF ISIAESTGLI VPIGDFVIRT ACEQLARWRN EGHDKLTLAV NISPVQFWRG DLIDTISRAL AETGIEARRL ELEITETAMM EYPELVSEKI VALKRLGIRV ALDDFGTGYS SLSYLHRFAV DTLKVDRSFI QAIPADRSVC VMVSSIVHLA RSLGLTVVVE GTETEEQIAW LAALGRIEAQ GFLFSRPVPS DGIAALLARF GVCGGDSARA AGRDIANAAK QSFGSD

NT Sequence

atgagttcgt ctcggtcaca cgccgcccgc gccgcgcggc cggtgcgcgc gacacgcaag tcgcgccgcc gccgcgcgct gttcgcgatt ccgctcctcg gcacgctggc gctcgcgctg ctgtgggtgg ccatcatcgc gcgcctgtcc gtcgagaagg acagcgcgtt ccgcgatgcg ggcgcgtcgg ccgcgatcct gtcgacggcg ctcgagcagc acacggtcaa ggcgatccat caggtcgacc agatcacccg cttcgtcaaa tacgaattcg agaagtcgcc cacccgcttc aaccttgcga gcacggtgga gaaaggcgtg gtgccgagcg acacgctgat ccaggtgtcc atcctcgatg cgcgcggccg gctcgtcgcg aatacggccg aggcgcatcc gaagccgatc gatctgtccg accgcgagca cttcaaggtg cacatgcagc gcaacgacga cgatctctac atcagcaagc cggtgttcgg ccgcgtgtcg cgccactgga cgctgcagat gacgcggcgc ctgaaccatc cggacggctc gttcgccggc gtggtcgtcg tgtccgagga tccggcctac ttcacgaacg acttctacaa caacgcgacg ctcggcaagg acggcgtgat cgcggtgatc tcggatacgg gcgccgtgct cgcgcgcagc acgggctcca cgcagaagac gcccggcacg ttctcggcat cgggcgtcta cccggtgtcg gagcgcgcct cgggcaccta tgtcgatccg atcgacggca tcgtgcgcat cgtgtcgtat cgccatctcg acggctatcc gctcggcgtg atggtgggcc tgtcgcaggc ggaagaattc gccgactacc accacacgcg caatgtctat ctgctgatga cgggcttcat cacgctcgcg atgctcgcgt tcttcggcgt cgcgacgggc ctgatcggca agctgctcgg ccgcgagcgc gaaatgacgc agctcgccga gttcgatctg ctgacggggc tcgcgaaccg ctatgcgacg ctgcgcagcc tgcgcaacga cgtcgcgctg ccgtcgaatc tgtcgcgcct cgcgatcctg ttcatcgatc tcgacaattt caagaccgtc aacgacacgc tcggccacaa cgcgggcgac atcgtgctgc agatgacggc cgcgcgcctg tccgatgcgg tggcgaacga aggcgcgctc gcgcgcatcg gcggcgacga gttcgtcgtc gtcgtgaagg gcgagaacgt cgagcggcgc gccgagcggc tcgcggaggc gatcgtgcga atgttcgccg agccgttcga cgtgcgcggc agctcgttcg tgctgcacgc gagcatcggc atcgcactgc attcggtgga gaacgaaagc gagatcgact tgctgaagaa ggccgatctc gcgatgtaca gcgccaagga cgccggcaag aactgctacc agttctatgc gccgcacttg tcgcaccgcg ccgatcacct gatgcgctgg gagcagcagt tgcgcgtcgc gctgtcggac gggcaactgt tcctcgccta ccagccgaag atcgatctca cgtaccgctg catcaccggc ttcgaggcgc tcgtgcgctg ggaccatccg gagcacggcg tgatctccgc gaacgaattc atttcgatcg ccgaatcgac cgggctcatc gtgccgatcg gcgacttcgt gatccgcacc gcgtgcgagc agctcgcgcg ctggcgcaac gagggccacg acaagctcac gctcgccgtc aatatctcgc ccgtgcagtt ctggcgcggc gacctgatcg acacgatctc gcgcgcgctc gccgagaccg gaatcgaggc gcgccgcctc gagctcgaga tcaccgagac cgcgatgatg gaataccctg agctcgtatc ggagaagatc gtcgcgctga aaaggctcgg gatccgcgtc gcgctcgacg atttcggcac cggctattcg tcgctgtcct acctgcaccg cttcgcggtc gacaccctga aggtcgaccg ctcgttcatc caggcgattc cggccgaccg cagcgtgtgt gtgatggtgt cgtcgatcgt gcacctcgcg cgctcgctcg ggctcacggt cgtcgtcgag gggacggaga cggaggagca gatcgcgtgg ctcgccgcgc tcgggcgcat cgaggcgcaa ggcttcctgt tctcgcggcc ggtgccctcc gacggcattg cggccctgct cgcgcgcttc ggcgtctgcg gcggcgacag cgcgcgcgcg gcgggccgcg acatcgcgaa cgccgctaaa cagtcgttcg gcagcgacta a

Details for BupsM.17236.g.B2.GE38813

HARVESTED ON:	1/22/2015
SEQUENCED ON:	1/15/2015
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMR LSVEKDSAFR DAGASAAILS TALEQHTVKA IHQVDQITRF VKYEFEKSPT RFNLASTVEK GVVPSDTLIQ VSILDARGRL VANTAEAHPK PIDLSDREHF KVHMQRNDDD LYISKPVFGR VSRHWTLQMT RRLNHPDGSF AGVVVVSEDP AYFTNDFYNN ATLGKDGVIA VISDTGAVLA RSTGSTQKTP GTFSASGVYP VSERASGTYV DPIDGIVRIV SYRHLDGYPL GVMVGLSQAE EFADYHHTRN

Validated NT Sequence

atggctcacc accaccacca ccatatgcgc ctgtccgtcg agaaggacag cgcgttccgc gatgcgggcg cgtcggccgc gatcctgtcg acggcgctcg agcagcacac ggtcaaggcg atccatcagg tcgaccagat cacccgcttc gtcaaatacg aattcgagaa gtcgcccacc cgcttcaacc ttgcgagcac ggtggagaaa ggcgtggtgc cgagcgacac gctgatccag gtgtccatcc tcgatgcgcg cggccggctc gtcgcgaata cggccgaggc gcatccgaag ccgatcgatc tgtccgaccg cgagcacttc aaggtgcaca tgcagcgcaa cgacgacgat ctctacatca gcaagccggt gttcggccgc gtgtcgcgcc actggacgct gcagatgacg cggcgcctga accatccgga cggctcgttc gccggcgtgg tcgtcgtgtc cgaggatccg gcctacttca cgaacgactt ctacaacaac gcgacgctcg gcaaggacgg cgtgatcgcg gtgatctcgg atacgggcgc cgtgctcgcg cgcagcacgg gctccacgca gaagacgccc ggcacgttct cggcatcggg cgtctacccg gtgtcggagc gcgcctcggg cacctatgtc gatccgatcg acggcatcgt gcgcatcgtg tcgtatcgcc atctcgacgg ctatccgctc ggcgtgatgg tgggcctgtc gcaggcggaa gaattcgccg actaccacca cacgcgcaat taagtgagta agat

Expected Protein Sequence

MAHHHHHHRL SVEKDSAFRD AGASAAILST ALEQHTVKAI HQVDQITRFV KYEFEKSPTR FNLASTVEKG VVPSDTLIQV SILDARGRLV ANTAEAHPKP IDLSDREHFK VHMQRNDDDL YISKPVFGRV SRHWTLQMTR RLNHPDGSFA GVVVVSEDPA YFTNDFYNNA TLGKDGVIAV ISDTGAVLAR STGSTQKTPG TFSASGVYPV SERASGTYVD PIDGIVRIVS YRHLDGYPLG VMVGLSQAEE FADYHHTRN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcgcctgt ccgtcgagaa ggacagcgcg ttccgcgatg cgggcgcgtc ggccgcgatc ctgtcgacgg cgctcgagca gcacacggtc aaggcgatcc atcaggtcga ccagatcacc cgcttcgtca aatacgaatt cgagaagtcg cccacccgct tcaaccttgc gagcacggtg gagaaaggcg tggtgccgag cgacacgctg atccaggtgt ccatcctcga tgcgcgcggc cggctcgtcg cgaatacggc cgaggcgcat ccgaagccga tcgatctgtc cgaccgcgag cacttcaagg tgcacatgca gcgcaacgac gacgatctct acatcagcaa gccggtgttc ggccgcgtgt cgcgccactg gacgctgcag atgacgcggc gcctgaacca tccggacggc tcgttcgccg gcgtggtcgt cgtgtccgag gatccggcct acttcacgaa cgacttctac aacaacgcga cgctcggcaa ggacggcgtg atcgcggtga tctcggatac gggcgccgtg ctcgcgcgca gcacgggctc cacgcagaag acgcccggca cgttctcggc atcgggcgtc tacccggtgt cggagcgcgc ctcgggcacc tatgtcgatc cgatcgacgg catcgtgcgc atcgtgtcgt atcgccatct cgacggctat ccgctcggcg tgatggtggg cctgtcgcag gcggaagaat tcgccgacta ccaccacacg cgcaattgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for BupsM.17236.g.B3.GE38814

HARVESTED ON:	1/22/2015
SEQUENCED ON:	1/15/2015
EXPECTED MW:	52kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMG KLLGREREMT QLAEFDLLTG LANRYATLRS LRNDVALPSN LSRLAILFID LDNFKTVNDT LGHNAGDIVL QMTAARLSDA VANEGALARI GGDEFVVVVK GENVERRAER LAEAIVRMFA EPFDVRGSSF VLHASIGIAL HSVENESEID LLKKADLAMY SAKDAGKNCY QFYAPHLSHR ADHLMRWEQQ LRVALSDGQL FLAYQPKIDL TYRCITGFEA LVRWDHPEHG VISANEFISI AESTGLIVPI GDFVIRTACE QLARWRNEGH DKLTLAVNIS PVQFWRGDLI DTISRALAET GIEARRLELE ITETAMMEYP ELVSEKIVAL KRLGIRVALD DFGTGYSSLS YLHRFAVDTL KVDRSFIQAI PADRSVCVMV SSIVHLARSL GLTVVVEGTE TEEQIAWLAA LGRIEAQGFL FSRPVPSDGI AALLARFGVC GGDSARAAGR DIANA

Validated NT Sequence

atggctcacc accaccacca ccatatgggc aagctgctcg gccgcgagcg cgaaatgacg cagctcgccg agttcgatct gctgacgggg ctcgcgaacc gctatgcgac gctgcgcagc ctgcgcaacg acgtcgcgct gccgtcgaat ctgtcgcgcc tcgcgatcct gttcatcgat ctcgacaatt tcaagaccgt caacgacacg ctcggccaca acgcgggcga catcgtgctg cagatgacgg ccgcgcgcct gtccgatgcg gtggcgaacg aaggcgcgct cgcgcgcatc ggcggcgacg agttcgtcgt cgtcgtgaag ggcgagaacg tcgagcggcg cgccgagcgg ctcgcggagg cgatcgtgcg aatgttcgcc gagccgttcg acgtgcgcgg cagctcgttc gtgctgcacg cgagcatcgg catcgcactg cattcggtgg agaacgaaag cgagatcgac ttgctgaaga aggccgatct cgcgatgtac agcgccaagg acgccggcaa gaactgctac cagttctatg cgccgcactt gtcgcaccgc gccgatcacc tgatgcgctg ggagcagcag ttgcgcgtcg cgctgtcgga cgggcaactg ttcctcgcct accagccgaa gatcgatctc acgtaccgct gcatcaccgg cttcgaggcg ctcgtgcgct gggaccatcc ggagcacggc gtgatctccg cgaacgaatt catttcgatc gccgaatcga ccgggctcat cgtgccgatc ggcgacttcg tgatccgcac cgcgtgcgag cagctcgcgc gctggcgcaa cgagggccac gacaagctca cgctcgccgt caatatctcg cccgtgcagt tctggcgcgg cgacctgatc gacacgatct cgcgcgcgct cgccgagacc ggaatcgagg cgcgccgcct cgagctcgag atcaccgaga ccgcgatgat ggaataccct gagctcgtat cggagaagat cgtcgcgctg aaaaggctcg ggatccgcgt cgcgctcgac gatttcggca ccggctattc gtcgctgtcc tacctgcacc gcttcgcggt cgacaccctg aaggtcgacc gctcgttcat ccaggcgatt ccggccgacc gcagcgtgtg tgtgatggtg tcgtcgatcg tgcacctcgc gcgctcgctc gggctcacgg tcgtcgtcga ggggacggag acggaggagc agatcgcgtg gctcgccgcg ctcgggcgca tcgaggcgca aggcttcctg ttctcgcggc cggtgccctc cgacggcatt gcggccctgc tcgcgcgctt cggcgtctgc ggcggcgaca gcgcgcgcgc ggcgggccgc gacatcgcga acgccgct

Expected Protein Sequence

MAHHHHHHGK LLGREREMTQ LAEFDLLTGL ANRYATLRSL RNDVALPSNL SRLAILFIDL DNFKTVNDTL GHNAGDIVLQ MTAARLSDAV ANEGALARIG GDEFVVVVKG ENVERRAERL AEAIVRMFAE PFDVRGSSFV LHASIGIALH SVENESEIDL LKKADLAMYS AKDAGKNCYQ FYAPHLSHRA DHLMRWEQQL RVALSDGQLF LAYQPKIDLT YRCITGFEAL VRWDHPEHGV ISANEFISIA ESTGLIVPIG DFVIRTACEQ LARWRNEGHD KLTLAVNISP VQFWRGDLID TISRALAETG IEARRLELEI TETAMMEYPE LVSEKIVALK RLGIRVALDD FGTGYSSLSY LHRFAVDTLK VDRSFIQAIP ADRSVCVMVS SIVHLARSLG LTVVVEGTET EEQIAWLAAL GRIEAQGFLF SRPVPSDGIA ALLARFGVCG GDSARAAGRD IANAAKQSFG SD

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggcaagc tgctcggccg cgagcgcgaa atgacgcagc tcgccgagtt cgatctgctg acggggctcg cgaaccgcta tgcgacgctg cgcagcctgc gcaacgacgt cgcgctgccg tcgaatctgt cgcgcctcgc gatcctgttc atcgatctcg acaatttcaa gaccgtcaac gacacgctcg gccacaacgc gggcgacatc gtgctgcaga tgacggccgc gcgcctgtcc gatgcggtgg cgaacgaagg cgcgctcgcg cgcatcggcg gcgacgagtt cgtcgtcgtc gtgaagggcg agaacgtcga gcggcgcgcc gagcggctcg cggaggcgat cgtgcgaatg ttcgccgagc cgttcgacgt gcgcggcagc tcgttcgtgc tgcacgcgag catcggcatc gcactgcatt cggtggagaa cgaaagcgag atcgacttgc tgaagaaggc cgatctcgcg atgtacagcg ccaaggacgc cggcaagaac tgctaccagt tctatgcgcc gcacttgtcg caccgcgccg atcacctgat gcgctgggag cagcagttgc gcgtcgcgct gtcggacggg caactgttcc tcgcctacca gccgaagatc gatctcacgt accgctgcat caccggcttc gaggcgctcg tgcgctggga ccatccggag cacggcgtga tctccgcgaa cgaattcatt tcgatcgccg aatcgaccgg gctcatcgtg ccgatcggcg acttcgtgat ccgcaccgcg tgcgagcagc tcgcgcgctg gcgcaacgag ggccacgaca agctcacgct cgccgtcaat atctcgcccg tgcagttctg gcgcggcgac ctgatcgaca cgatctcgcg cgcgctcgcc gagaccggaa tcgaggcgcg ccgcctcgag ctcgagatca ccgagaccgc gatgatggaa taccctgagc tcgtatcgga gaagatcgtc gcgctgaaaa ggctcgggat ccgcgtcgcg ctcgacgatt tcggcaccgg ctattcgtcg ctgtcctacc tgcaccgctt cgcggtcgac accctgaagg tcgaccgctc gttcatccag gcgattccgg ccgaccgcag cgtgtgtgtg atggtgtcgt cgatcgtgca cctcgcgcgc tcgctcgggc tcacggtcgt cgtcgagggg acggagacgg aggagcagat cgcgtggctc gccgcgctcg ggcgcatcga ggcgcaaggc ttcctgttct cgcggccggt gccctccgac ggcattgcgg ccctgctcgc gcgcttcggc gtctgcggcg gcgacagcgc gcgcgcggcg ggccgcgaca tcgcgaacgc cgctaaacag tcgttcggca gcgactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for BupsM.17236.g.B3.PS38089

PURIFICATION DATe:	10/26/2016
CONCENTRATION:	4.5mg/ml
OBSERVED MW:	43kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	SEC
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	2
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGGCAAGC TGCTCGGCCG CGAGCGCGAA ATGACGCAGC TCGCCGAGTT CGATCTGCTG ACGGGGCTCG CGAACCGCTA TGCGACGCTG CGCAGCCTGC GCAACGACGT CGCGCTGCCG TCGAATCTGT CGCGCCTCGC GATCCTGTTC ATCGATCTCG ACAATTTCAA GACCGTCAAC GACACGCTCG GCCACAACGC GGGCGACATC GTGCTGCAGA TGACGGCCGC GCGCCTGTCC GATGCGGTGG CGAACGAAGG CGCGCTCGCG CGCATCGGCG GCGACGAGTT CGTCGTCGTC GTGAAGGGCG AGAACGTCGA GCGGCGCGCC GAGCGGCTCG CGGAGGCGAT CGTGCGAATG TTCGCCGAGC CGTTCGACGT GCGCGGCAGC TCGTTCGTGC TGCACGCGAG CATCGGCATC GCACTGCATT CGGTGGAGAA CGAAAGCGAG ATCGACTTGC TGAAGAAGGC CGATCTCGCG ATGTACAGCG CCAAGGACGC CGGCAAGAAC TGCTACCAGT TCTATGCGCC GCACTTGTCG CACCGCGCCG ATCACCTGAT GCGCTGGGAG CAGCAGTTGC GCGTCGCGCT GTCGGACGGG CAACTGTTCC TCGCCTACCA GCCGAAGATC GATCTCACGT ACCGCTGCAT CACCGGCTTC GAGGCGCTCG TGCGCTGGGA CCATCCGGAG CACGGCGTGA TCTCCGCGAA CGAATTCATT TCGATCGCCG AATCGACCGG GCTCATCGTG CCGATCGGCG ACTTCGTGAT CCGCACCGCG TGCGAGCAGC TCGCGCGCTG GCGCAACGAG GGCCACGACA AGCTCACGCT CGCCGTCAAT ATCTCGCCCG TGCAGTTCTG GCGCGGCGAC CTGATCGACA CGATCTCGCG CGCGCTCGCC GAGACCGGAA TCGAGGCGCG CCGCCTCGAG CTCGAGATCA CCGAGACCGC GATGATGGAA TACCCTGAGC TCGTATCGGA GAAGATCGTC GCGCTGAAAA GGCTCGGGAT CCGCGTCGCG CTCGACGATT TCGGCACCGG CTATTCGTCG CTGTCCTACC TGCACCGCTT CGCGGTCGAC ACCCTGAAGG TCGACCGCTC GTTCATCCAG GCGATTCCGG CCGACCGCAG CGTGTGTGTG ATGGTGTCGT CGATCGTGCA CCTCGCGCGC TCGCTCGGGC TCACGGTCGT CGTCGAGGGG ACGGAGACGG AGGAGCAGAT CGCGTGGCTC GCCGCGCTCG GGCGCATCGA GGCGCAAGGC TTCCTGTTCT CGCGGCCGGT GCCCTCCGAC GGCATTGCGG CCCTGCTCGC GCGCTTCGGC GTCTGCGGCG GCGACAGCGC GCGCGCGGCG GGCCGCGACA TCGCGAACGC CGCTAAACAG TCGTTCGGCA GCGACTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT