BupsM.17236.f

EAL domain-containing protein

CENTER ID: BupsM.17236.f
ORGANISM: Burkholderia pseudomallei 1026b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsM.17236.f.B1.GE38812 full length 1 611
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|884204.6.peg.2142
RefSeq: YP_006275289.1
RefSeq: WP_004521882.1
UniProt: A0A0H3HLT9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEAIRNKTPR KGSWRAALHQ LRRFAWSGGA LMPARAGTPR ADDTVRSWLE QTVGAVDFLA HVDRDLRFLY VSDASLRFIG YHRDYLHTLT LRELIPEQDT ATLDGLLARA SQTGDVEKAT LCLVKSLTYP LDVEVRAVRS RHHGVDGFAI AAFDVSSWRA IEARLTYELH HDPMTGLDNL SALLPALMRA QQIADEGGGC TALLLLDLDD YQRINRALGY DAGDALLRDT AQRLQRLATR GERLARVASD KFAVMLSAPT RVAALDAAEA LARQLQTAVQ RPYAYDGQAV HLSASVGIAI YPDARATSKH AQHHSPLLRR ADRALAQAKA AGGNALAFHQ PTDDPADAER LKLEADLYNG VRNGEFSLHF QPITKSLSGA VVGVEALIRW HHPVHGLVAP ATFIPLAESI GLINYLGNWV LKAACMQLVS WDRQGIALQY VAVNVSPQQF RDPRFTQSVR DAIKLTGIDP RRIVLEITES LLMHDPNHAK TLLEEVTELG IRFAVDDFGT GYSSLAYLQS FPLAKLKIDR SFVENLLTSR NDRAIVSAVV GLAQTLELEL VAEGVETEAQ RTLLTEMGCN HIQGWLVCQA LPSDELAQRF EAQELHLHAT A
NT Sequence
atggaagcca tcaggaacaa aacgccccgc aagggctcct ggcgcgccgc acttcaccag ctccgccgct tcgcctggtc gggcggcgcg ctgatgcccg cgcgcgccgg tacgccgcgc gccgacgaca cagttcgaag ctggctggag caaaccgtcg gcgcggtcga tttcctcgcc cacgtggacc gggacctgcg cttcctgtac gtgtccgatg ccagcctgcg cttcatcggt tatcaccgcg actatctgca cacgctgacg ctgcgcgagc tgatccccga gcaggatacc gcgacgctcg acggcctgct cgcgcgcgcg tcgcagacgg gcgatgtcga gaaggcgacg ctctgcctcg tgaaatcgct gacctatccg ctcgacgtcg aggtgcgcgc ggtgcgcagc cgccaccacg gcgtcgacgg cttcgcgatc gccgcgttcg acgtgtcgtc gtggcgcgcg atcgaggccc ggctcaccta cgagctgcac cacgatccga tgacggggct cgataacctg tcggcgctcc tgcccgcgct gatgcgcgcg cagcagatcg ccgacgaagg cggcggctgc acggcgctgc tgctgctcga tctcgacgat taccagcgga tcaaccgcgc gctcggctac gacgcgggcg acgcgctgct gcgcgacacc gcgcagcggc tgcaacggct cgcgacccgc ggcgagcggc tcgcgcgggt cgcgagcgac aagttcgcgg tgatgctgag cgcgcccacg cgcgtcgccg cgctcgacgc ggccgaagcg ctcgcgcgcc agctgcagac ggccgtccag cggccgtacg cgtatgacgg gcaggccgtg cacctgtcgg cgagcgtcgg catcgcgatc tatccggacg cgcgcgcgac gtcgaagcac gcgcagcacc acagcccgct gctgcggcgc gcggaccgcg cgctcgcgca ggcgaaggcg gcgggcggca acgcgctcgc gttccaccag ccgaccgacg atccggccga cgccgagcgc ctgaagctcg aggccgatct ctacaacggc gtgcgcaacg gcgagttctc gctgcatttc cagccgatca cgaagagcct gtcgggcgcc gtggtcggcg tcgaggcgct gatccgctgg caccatccgg tgcacgggct cgtcgcgccc gcgacgttca tcccgctcgc cgaatcgatc ggcctcatca actatctcgg caactgggtg ctgaaggccg cgtgcatgca gctcgtgtcg tgggaccggc aaggcatcgc gctgcagtac gtggccgtca acgtgtcgcc gcagcagttc cgcgatccgc gtttcacgca gagcgtgcgc gacgcgatca agctgacggg catcgatccg cgccgcatcg ttctcgagat caccgaaagc ctgctgatgc acgatccgaa tcatgcgaag acgctgctcg aggaagtgac cgagctcggc atccggttcg cggtcgacga tttcggcacc ggctactcga gcctcgcgta cctgcagagc ttcccgctcg cgaagctgaa gatcgaccgc agtttcgtcg aaaatctgtt gacgtccaga aacgaccgcg cgatcgtgtc ggcggtcgtc ggcctcgcgc agacgctcga gctcgagctc gtcgcggagg gcgtcgagac cgaggcgcag cgcacgctgc tcaccgagat gggctgcaac cacattcaag gctggctcgt ctgccaggcg ctgccgtccg acgaactcgc gcaacgcttc gaggcacagg agctccatct gcacgccacc gcatga
Details for BupsM.17236.f.B1.GE38812
HARVESTED ON: 1/22/2015
SEQUENCED ON: 1/15/2015
EXPECTED MW: 68kDa
OBSERVED MW: 65kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 96
PERCENT COVERAGE: 42
Validated AA Sequence
MAHHHHHHME AIRNKTPRKG SWRAALHQLR RFAWSGGALM PARAGTPRAD DTVRSWLEQT VGAVDFLAHV DRDLRFLYVS DASLRFIGYH RDYLHTLTLR ELIPEQDTAT LDGLLARASQ TGDVEKATLC LVKSLTYPLD VEVRAVRSRH HGVDGFAIAA FDVSSWRAIE ARLTYELHHD PMTGLDNLSA LLPALMRAQQ IADEGGGCTA LLLLDLDDYQ RINRALGYDA GDALLRDTAQ RXXXLATRGE RLARXXSXXX X
Validated NT Sequence
atggctcacc accaccacca ccatatggaa gccatcagga acaaaacgcc ccgcaagggc tcctggcgcg ccgcacttca ccagctccgc cgcttcgcct ggtcgggcgg cgcgctgatg cccgcgcgcg ccggtacgcc gcgcgccgac gacacagttc gaagctggct ggagcaaacc gtcggcgcgg tcgatttcct cgcccacgtg gaccgggacc tgcgcttcct gtacgtgtcc gatgccagcc tgcgcttcat cggttatcac cgcgactatc tgcacacgct gacgctgcgc gagctgatcc ccgagcagga taccgcgacg ctcgacggcc tgctcgcgcg cgcgtcgcag acgggcgatg tcgagaaggc gacgctctgc ctcgtgaaat cgctgaccta tccgctcgac gtcgaggtgc gcgcggtgcg cagccgccac cacggcgtcg acggcttcgc gatcgccgcg ttcgacgtgt cgtcgtggcg cgcgatcgag gcccggctca cctacgagct gcaccacgat ccgatgacgg ggctcgataa cctgtcggcg ctcctgcccg cgctgatgcg cgcgcagcag atcgccgacg aaggcggcgg ctgcacggcg ctgctgctgc tcgatctcga cgattaccag cggatcaacc gcgcgctcgg ctacgacgcg ggcgacgcgc tgctgcgcga caccgcgcag cggcnnnnnn ggctcgcgac ccgcggcgag cggctcgcgc ggnnnnngag cgnnnngnnn nngg
Expected Protein Sequence
MAHHHHHHME AIRNKTPRKG SWRAALHQLR RFAWSGGALM PARAGTPRAD DTVRSWLEQT VGAVDFLAHV DRDLRFLYVS DASLRFIGYH RDYLHTLTLR ELIPEQDTAT LDGLLARASQ TGDVEKATLC LVKSLTYPLD VEVRAVRSRH HGVDGFAIAA FDVSSWRAIE ARLTYELHHD PMTGLDNLSA LLPALMRAQQ IADEGGGCTA LLLLDLDDYQ RINRALGYDA GDALLRDTAQ RLQRLATRGE RLARVASDKF AVMLSAPTRV AALDAAEALA RQLQTAVQRP YAYDGQAVHL SASVGIAIYP DARATSKHAQ HHSPLLRRAD RALAQAKAAG GNALAFHQPT DDPADAERLK LEADLYNGVR NGEFSLHFQP ITKSLSGAVV GVEALIRWHH PVHGLVAPAT FIPLAESIGL INYLGNWVLK AACMQLVSWD RQGIALQYVA VNVSPQQFRD PRFTQSVRDA IKLTGIDPRR IVLEITESLL MHDPNHAKTL LEEVTELGIR FAVDDFGTGY SSLAYLQSFP LAKLKIDRSF VENLLTSRND RAIVSAVVGL AQTLELELVA EGVETEAQRT LLTEMGCNHI QGWLVCQALP SDELAQRFEA QELHLHATA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaag ccatcaggaa caaaacgccc cgcaagggct cctggcgcgc cgcacttcac cagctccgcc gcttcgcctg gtcgggcggc gcgctgatgc ccgcgcgcgc cggtacgccg cgcgccgacg acacagttcg aagctggctg gagcaaaccg tcggcgcggt cgatttcctc gcccacgtgg accgggacct gcgcttcctg tacgtgtccg atgccagcct gcgcttcatc ggttatcacc gcgactatct gcacacgctg acgctgcgcg agctgatccc cgagcaggat accgcgacgc tcgacggcct gctcgcgcgc gcgtcgcaga cgggcgatgt cgagaaggcg acgctctgcc tcgtgaaatc gctgacctat ccgctcgacg tcgaggtgcg cgcggtgcgc agccgccacc acggcgtcga cggcttcgcg atcgccgcgt tcgacgtgtc gtcgtggcgc gcgatcgagg cccggctcac ctacgagctg caccacgatc cgatgacggg gctcgataac ctgtcggcgc tcctgcccgc gctgatgcgc gcgcagcaga tcgccgacga aggcggcggc tgcacggcgc tgctgctgct cgatctcgac gattaccagc ggatcaaccg cgcgctcggc tacgacgcgg gcgacgcgct gctgcgcgac accgcgcagc ggctgcaacg gctcgcgacc cgcggcgagc ggctcgcgcg ggtcgcgagc gacaagttcg cggtgatgct gagcgcgccc acgcgcgtcg ccgcgctcga cgcggccgaa gcgctcgcgc gccagctgca gacggccgtc cagcggccgt acgcgtatga cgggcaggcc gtgcacctgt cggcgagcgt cggcatcgcg atctatccgg acgcgcgcgc gacgtcgaag cacgcgcagc accacagccc gctgctgcgg cgcgcggacc gcgcgctcgc gcaggcgaag gcggcgggcg gcaacgcgct cgcgttccac cagccgaccg acgatccggc cgacgccgag cgcctgaagc tcgaggccga tctctacaac ggcgtgcgca acggcgagtt ctcgctgcat ttccagccga tcacgaagag cctgtcgggc gccgtggtcg gcgtcgaggc gctgatccgc tggcaccatc cggtgcacgg gctcgtcgcg cccgcgacgt tcatcccgct cgccgaatcg atcggcctca tcaactatct cggcaactgg gtgctgaagg ccgcgtgcat gcagctcgtg tcgtgggacc ggcaaggcat cgcgctgcag tacgtggccg tcaacgtgtc gccgcagcag ttccgcgatc cgcgtttcac gcagagcgtg cgcgacgcga tcaagctgac gggcatcgat ccgcgccgca tcgttctcga gatcaccgaa agcctgctga tgcacgatcc gaatcatgcg aagacgctgc tcgaggaagt gaccgagctc ggcatccggt tcgcggtcga cgatttcggc accggctact cgagcctcgc gtacctgcag agcttcccgc tcgcgaagct gaagatcgac cgcagtttcg tcgaaaatct gttgacgtcc agaaacgacc gcgcgatcgt gtcggcggtc gtcggcctcg cgcagacgct cgagctcgag ctcgtcgcgg agggcgtcga gaccgaggcg cagcgcacgc tgctcaccga gatgggctgc aaccacattc aaggctggct cgtctgccag gcgctgccgt ccgacgaact cgcgcaacgc ttcgaggcac aggagctcca tctgcacgcc accgcatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t