BupsM.17236.e

GGDEF domain-containing protein

CENTER ID: BupsM.17236.e
ORGANISM: Burkholderia pseudomallei 1026b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BupsM.17236.e.AE1.GE44038 full length 1 311
BupsM.17236.e.B1.GE38811 full length 1 311
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BupsM.17236.e.B1.PW37753 full length 1 311

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|884204.6.peg.7113
RefSeq: YP_006279130.1
RefSeq: WP_004538021.1
UniProt: A0A0H3HXU6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNLLSSLSDL VIERVSFGIF VLDREMRVLM WNRFMQDHSG VAAADVIGRN LFDSFPELPR AWLSRKIESV FQLGSFAFSS WEQRPYLFRF DHDRPITGGV DFMQQDCTFV PIARAREVEA VCVTVSDVTH VSVMQREREE AVAKLQEYAN RDGLTGIANR RFFEARLRDE FSRWQRYGGN LSVLLFDLDH FKTINDRFGH VVGDNVLRVM AQRVADVVRS QDTFGRFGGE EFALLLPCTS LDEAMHVAEK IRQTIGSTPI EAEGVRVPVT ASVGGAAAHA GALTHETLVN EADAALYRAK RQGRNCSVAF G
NT Sequence
atgaacctgc tttcctcgct gagcgatctc gtcatcgaac gggtcagctt cggtattttc gtgctcgacc gcgagatgag ggtactcatg tggaaccggt tcatgcagga ccatagcggc gtcgcggcgg ccgacgtgat cggccgcaac ctgttcgaca gctttcccga gctgccgcgc gcgtggctgt cgcgcaagat cgagagcgtg ttccagctcg gcagcttcgc gttcagctcg tgggagcagc gcccgtatct gttccgcttc gatcacgatc ggccgatcac gggcggcgtc gacttcatgc agcaggactg cacgttcgtg ccgatcgcgc gcgcgcgcga agtggaggcg gtctgcgtga ccgtctccga tgtcacgcac gtgagcgtga tgcagcgcga gcgcgaggag gccgtcgcga agctgcagga atacgcgaac cgcgacggcc tgacgggcat cgcgaaccgg cgcttcttcg aggcgcggct gcgcgacgaa ttctcgcgct ggcagcgcta cggcggcaat ctgtcggtgc tgctgttcga tctcgaccat ttcaagacga tcaacgatcg cttcggccac gtcgtcgggg acaacgtgct gcgcgtgatg gcgcagcgtg tcgcggacgt cgtgcgctcg caggacacgt tcggccgctt cggcggcgag gaattcgcgc tgctgctgcc gtgcacgtcg ctcgacgagg cgatgcacgt tgccgagaag atccggcaga cgatcggcag caccccgatc gaagcggagg gcgtgcgggt gccggtgacg gcgagcgtcg gcggcgcggc cgcgcacgcc ggcgcgctca cgcacgagac gctcgtcaac gaggccgacg cggcgcttta tcgcgcgaaa cggcagggac ggaactgctc ggttgcgttc ggttga
Details for BupsM.17236.e.AE1.GE44038
HARVESTED ON: 1/7/2021
SEQUENCED ON: 1/8/2021
EXPECTED MW: 36kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 22
PERCENT COVERAGE: 100
Validated AA Sequence
MMMMVVPSTE RNRAVPSLPF RAIKRRVGLV DERLVRERAG VRGRAADARR HRHPHALRFD RGAADRLPDL LGNVHRLVER RARQQQREFL AAEAAERVLR AHDVRDTLRH HAQHVVPDDV AEAIVDRLEM VEIEQQHRQI AAVALPAREF VAQPRLEEAP VRDARQAVAV RVFLQLRDGL LALALHHAHV RDIGDGHADR LHFARARDRH ERAVLLHEVD AARDRPIVIE AEQIRALLPR AEREAAELEH ALDLARQPRA RQLGKAVEQV AADHVGRRDA AMVLHEPVPH EYPHLAVEHE NTEADPFDDE IAQRGKQVHG R
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccag caccgaacgc aaccgagcag ttccgtccct gccgtttcgc gcgataaagc gccgcgtcgg cctcgttgac gagcgtctcg tgcgtgagcg cgccggcgtg cgcggccgcg ccgccgacgc tcgccgtcac cggcacccgc acgccctccg cttcgatcgg ggtgctgccg atcgtctgcc ggatcttctc ggcaacgtgc atcgcctcgt cgagcgacgt gcacggcagc agcagcgcga attcctcgcc gccgaagcgg ccgaacgtgt cctgcgagcg cacgacgtcc gcgacacgct gcgccatcac gcgcagcacg ttgtccccga cgacgtggcc gaagcgatcg ttgatcgtct tgaaatggtc gagatcgaac agcagcaccg acagattgcc gccgtagcgc tgccagcgcg agaattcgtc gcgcagccgc gcctcgaaga agcgccggtt cgcgatgccc gtcaggccgt cgcggttcgc gtattcctgc agcttcgcga cggcctcctc gcgctcgcgc tgcatcacgc tcacgtgcgt gacatcggag acggtcacgc agaccgcctc cacttcgcgc gcgcgcgcga tcggcacgaa cgtgcagtcc tgctgcatga agtcgacgcc gcccgtgatc ggccgatcgt gatcgaagcg gaacagatac gggcgctgct cccacgagct gaacgcgaag ctgccgagct ggaacacgct ctcgatcttg cgcgacagcc acgcgcgcgg cagctcggga aagctgtcga acaggttgcg gccgatcacg tcggccgccg cgacgccgct atggtcctgc atgaaccggt tccacatgag taccctcatc tcgcggtcga gcacgaaaat accgaagctg acccgttcga tgacgagatc gctcagcgag gaaagcaggt tcatgggaga ga
Expected Protein Sequence
MNLLSSLSDL VIERVSFGIF VLDREMRVLM WNRFMQDHSG VAAADVIGRN LFDSFPELPR AWLSRKIESV FQLGSFAFSS WEQRPYLFRF DHDRPITGGV DFMQQDCTFV PIARAREVEA VCVTVSDVTH VSVMQREREE AVAKLQEYAN RDGLTGIANR RFFEARLRDE FSRWQRYGGN LSVLLFDLDH FKTINDRFGH VVGDNVLRVM AQRVADVVRS QDTFGRFGGE EFALLLPCTS LDEAMHVAEK IRQTIGSTPI EAEGVRVPVT ASVGGAAAHA GALTHETLVN EADAALYRAK RQGRNCSVAF GGHHHHHH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat gaacctgctt tcctcgctga gcgatctcgt catcgaacgg gtcagcttcg gtattttcgt gctcgaccgc gagatgaggg tactcatgtg gaaccggttc atgcaggacc atagcggcgt cgcggcggcc gacgtgatcg gccgcaacct gttcgacagc tttcccgagc tgccgcgcgc gtggctgtcg cgcaagatcg agagcgtgtt ccagctcggc agcttcgcgt tcagctcgtg ggagcagcgc ccgtatctgt tccgcttcga tcacgatcgg ccgatcacgg gcggcgtcga cttcatgcag caggactgca cgttcgtgcc gatcgcgcgc gcgcgcgaag tggaggcggt ctgcgtgacc gtctccgatg tcacgcacgt gagcgtgatg cagcgcgagc gcgaggaggc cgtcgcgaag ctgcaggaat acgcgaaccg cgacggcctg acgggcatcg cgaaccggcg cttcttcgag gcgcggctgc gcgacgaatt ctcgcgctgg cagcgctacg gcggcaatct gtcggtgctg ctgttcgatc tcgaccattt caagacgatc aacgatcgct tcggccacgt cgtcggggac aacgtgctgc gcgtgatggc gcagcgtgtc gcggacgtcg tgcgctcgca ggacacgttc ggccgcttcg gcggcgagga attcgcgctg ctgctgccgt gcacgtcgct cgacgaggcg atgcacgttg ccgagaagat ccggcagacg atcggcagca ccccgatcga agcggagggc gtgcgggtgc cggtgacggc gagcgtcggc ggcgcggccg cgcacgccgg cgcgctcacg cacgagacgc tcgtcaacga ggccgacgcg gcgctttatc gcgcgaaacg gcagggacgg aactgctcgg ttgcgttcgg tgggcaccac catcatcatc attaacggat ccgaattcga gctccgtcga caagcttgcg gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggat
Details for BupsM.17236.e.B1.GE38811
HARVESTED ON: 1/22/2015
SEQUENCED ON: 1/15/2015
EXPECTED MW: 36kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMN LLSSLSDLVI ERVSFGIFVL DREMRVLMWN RFMQDHSGVA AADVIGRNLF DSFPELPRAW LSRKIESVFQ LGSFAFSSWE QRPYLFRFDH DRPITGGVDF MQQDCTFVPI ARAREVEAVC VTVSDVTHVS VMQREREEAV AKLQEYANRD GLTGIANRRF FEARLRDEFS RWQRYGGNLS VLLFDLDHFK TINDRFGHVV GDNVLRVMAQ RVADVVRSQD TFGRFGGEEF ALLLPCTSLD EAMHVAEKIR QTIGSTPIEA EGVRVPVTAS VGGAAAHAGA LTHETLVNEA DAALYRAKRQ GRXXSVAFG
Validated NT Sequence
atggctcacc accaccacca ccatatgaac ctgctttcct cgctgagcga tctcgtcatc gaacgggtca gcttcggtat tttcgtgctc gaccgcgaga tgagggtact catgtggaac cggttcatgc aggaccatag cggcgtcgcg gcggccgacg tgatcggccg caacctgttc gacagctttc ccgagctgcc gcgcgcgtgg ctgtcgcgca agatcgagag cgtgttccag ctcggcagct tcgcgttcag ctcgtgggag cagcgcccgt atctgttccg cttcgatcac gatcggccga tcacgggcgg cgtcgacttc atgcagcagg actgcacgtt cgtgccgatc gcgcgcgcgc gcgaagtgga ggcggtctgc gtgaccgtct ccgatgtcac gcacgtgagc gtgatgcagc gcgagcgcga ggaggccgtc gcgaagctgc aggaatacgc gaaccgcgac ggcctgacgg gcatcgcgaa ccggcgcttc ttcgaggcgc ggctgcgcga cgaattctcg cgctggcagc gctacggcgg caatctgtcg gtgctgctgt tcgatctcga ccatttcaag acgatcaacg atcgcttcgg ccacgtcgtc ggggacaacg tgctgcgcgt gatggcgcag cgtgtcgcgg acgtcgtgcg ctcgcaggac acgttcggcc gcttcggcgg cgaggaattc gcgctgctgc tgccgtgcac gtcgctcgac gaggcgatgc acgttgccga gaagatccgg cagacgatcg gcagcacccc gatcgaagcg gagggcgtgc gggtgccggt gacggcgagc gtcggcggcg cggccgcgca cgccggcgcg ctcacgcacg agacgctcgt caacgaggcc gacgcggcgc tttatcgcgc gaaacggcag ggacgnnnnn nctcggttgc gttcggttaa gtgagtaa
Expected Protein Sequence
MAHHHHHHMN LLSSLSDLVI ERVSFGIFVL DREMRVLMWN RFMQDHSGVA AADVIGRNLF DSFPELPRAW LSRKIESVFQ LGSFAFSSWE QRPYLFRFDH DRPITGGVDF MQQDCTFVPI ARAREVEAVC VTVSDVTHVS VMQREREEAV AKLQEYANRD GLTGIANRRF FEARLRDEFS RWQRYGGNLS VLLFDLDHFK TINDRFGHVV GDNVLRVMAQ RVADVVRSQD TFGRFGGEEF ALLLPCTSLD EAMHVAEKIR QTIGSTPIEA EGVRVPVTAS VGGAAAHAGA LTHETLVNEA DAALYRAKRQ GRNCSVAFG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacc tgctttcctc gctgagcgat ctcgtcatcg aacgggtcag cttcggtatt ttcgtgctcg accgcgagat gagggtactc atgtggaacc ggttcatgca ggaccatagc ggcgtcgcgg cggccgacgt gatcggccgc aacctgttcg acagctttcc cgagctgccg cgcgcgtggc tgtcgcgcaa gatcgagagc gtgttccagc tcggcagctt cgcgttcagc tcgtgggagc agcgcccgta tctgttccgc ttcgatcacg atcggccgat cacgggcggc gtcgacttca tgcagcagga ctgcacgttc gtgccgatcg cgcgcgcgcg cgaagtggag gcggtctgcg tgaccgtctc cgatgtcacg cacgtgagcg tgatgcagcg cgagcgcgag gaggccgtcg cgaagctgca ggaatacgcg aaccgcgacg gcctgacggg catcgcgaac cggcgcttct tcgaggcgcg gctgcgcgac gaattctcgc gctggcagcg ctacggcggc aatctgtcgg tgctgctgtt cgatctcgac catttcaaga cgatcaacga tcgcttcggc cacgtcgtcg gggacaacgt gctgcgcgtg atggcgcagc gtgtcgcgga cgtcgtgcgc tcgcaggaca cgttcggccg cttcggcggc gaggaattcg cgctgctgct gccgtgcacg tcgctcgacg aggcgatgca cgttgccgag aagatccggc agacgatcgg cagcaccccg atcgaagcgg agggcgtgcg ggtgccggtg acggcgagcg tcggcggcgc ggccgcgcac gccggcgcgc tcacgcacga gacgctcgtc aacgaggccg acgcggcgct ttatcgcgcg aaacggcagg gacggaactg ctcggttgcg ttcggttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for BupsM.17236.e.B1.PW37753
PURIFICATION DATe: 7/29/2015
CONCENTRATION: 5mg/ml
OBSERVED MW: 35kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 12
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMN LLSSLSDLVI ERVSFGIFVL DREMRVLMWN RFMQDHSGVA AADVIGRNLF DSFPELPRAW LSRKIESVFQ LGSFAFSSWE QRPYLFRFDH DRPITGGVDF MQQDCTFVPI ARAREVEAVC VTVSDVTHVS VMQREREEAV AKLQEYANRD GLTGIANRRF FEARLRDEFS RWQRYGGNLS VLLFDLDHFK TINDRFGHVV GDNVLRVMAQ RVADVVRSQD TFGRFGGEEF ALLLPCTSLD EAMHVAEKIR QTIGSTPIEA EGVRVPVTAS VGGAAAHAGA LTHETLVNEA DAALYRAKRQ GRXXSVAFG
Validated NT Sequence
atggctcacc accaccacca ccatatgaac ctgctttcct cgctgagcga tctcgtcatc gaacgggtca gcttcggtat tttcgtgctc gaccgcgaga tgagggtact catgtggaac cggttcatgc aggaccatag cggcgtcgcg gcggccgacg tgatcggccg caacctgttc gacagctttc ccgagctgcc gcgcgcgtgg ctgtcgcgca agatcgagag cgtgttccag ctcggcagct tcgcgttcag ctcgtgggag cagcgcccgt atctgttccg cttcgatcac gatcggccga tcacgggcgg cgtcgacttc atgcagcagg actgcacgtt cgtgccgatc gcgcgcgcgc gcgaagtgga ggcggtctgc gtgaccgtct ccgatgtcac gcacgtgagc gtgatgcagc gcgagcgcga ggaggccgtc gcgaagctgc aggaatacgc gaaccgcgac ggcctgacgg gcatcgcgaa ccggcgcttc ttcgaggcgc ggctgcgcga cgaattctcg cgctggcagc gctacggcgg caatctgtcg gtgctgctgt tcgatctcga ccatttcaag acgatcaacg atcgcttcgg ccacgtcgtc ggggacaacg tgctgcgcgt gatggcgcag cgtgtcgcgg acgtcgtgcg ctcgcaggac acgttcggcc gcttcggcgg cgaggaattc gcgctgctgc tgccgtgcac gtcgctcgac gaggcgatgc acgttgccga gaagatccgg cagacgatcg gcagcacccc gatcgaagcg gagggcgtgc gggtgccggt gacggcgagc gtcggcggcg cggccgcgca cgccggcgcg ctcacgcacg agacgctcgt caacgaggcc gacgcggcgc tttatcgcgc gaaacggcag ggacgnnnnn nctcggttgc gttcggttaa gtgagtaa
Expressed Protein Sequence
MAHHHHHHMN LLSSLSDLVI ERVSFGIFVL DREMRVLMWN RFMQDHSGVA AADVIGRNLF DSFPELPRAW LSRKIESVFQ LGSFAFSSWE QRPYLFRFDH DRPITGGVDF MQQDCTFVPI ARAREVEAVC VTVSDVTHVS VMQREREEAV AKLQEYANRD GLTGIANRRF FEARLRDEFS RWQRYGGNLS VLLFDLDHFK TINDRFGHVV GDNVLRVMAQ RVADVVRSQD TFGRFGGEEF ALLLPCTSLD EAMHVAEKIR QTIGSTPIEA EGVRVPVTAS VGGAAAHAGA LTHETLVNEA DAALYRAKRQ GRNCSVAFG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACC TGCTTTCCTC GCTGAGCGAT CTCGTCATCG AACGGGTCAG CTTCGGTATT TTCGTGCTCG ACCGCGAGAT GAGGGTACTC ATGTGGAACC GGTTCATGCA GGACCATAGC GGCGTCGCGG CGGCCGACGT GATCGGCCGC AACCTGTTCG ACAGCTTTCC CGAGCTGCCG CGCGCGTGGC TGTCGCGCAA GATCGAGAGC GTGTTCCAGC TCGGCAGCTT CGCGTTCAGC TCGTGGGAGC AGCGCCCGTA TCTGTTCCGC TTCGATCACG ATCGGCCGAT CACGGGCGGC GTCGACTTCA TGCAGCAGGA CTGCACGTTC GTGCCGATCG CGCGCGCGCG CGAAGTGGAG GCGGTCTGCG TGACCGTCTC CGATGTCACG CACGTGAGCG TGATGCAGCG CGAGCGCGAG GAGGCCGTCG CGAAGCTGCA GGAATACGCG AACCGCGACG GCCTGACGGG CATCGCGAAC CGGCGCTTCT TCGAGGCGCG GCTGCGCGAC GAATTCTCGC GCTGGCAGCG CTACGGCGGC AATCTGTCGG TGCTGCTGTT CGATCTCGAC CATTTCAAGA CGATCAACGA TCGCTTCGGC CACGTCGTCG GGGACAACGT GCTGCGCGTG ATGGCGCAGC GTGTCGCGGA CGTCGTGCGC TCGCAGGACA CGTTCGGCCG CTTCGGCGGC GAGGAATTCG CGCTGCTGCT GCCGTGCACG TCGCTCGACG AGGCGATGCA CGTTGCCGAG AAGATCCGGC AGACGATCGG CAGCACCCCG ATCGAAGCGG AGGGCGTGCG GGTGCCGGTG ACGGCGAGCG TCGGCGGCGC GGCCGCGCAC GCCGGCGCGC TCACGCACGA GACGCTCGTC AACGAGGCCG ACGCGGCGCT TTATCGCGCG AAACGGCAGG GACGGAACTG CTCGGTTGCG TTCGGTTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T