BupsM.17236.d

EAL/GGDEF domain-containing protein

CENTER ID: BupsM.17236.d
ORGANISM: Burkholderia pseudomallei 1026b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BupsM.17236.d.B2.GE38810 soluble domain 233 688
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|884204.6.peg.6019
RefSeq: YP_006277536.1
RefSeq: WP_004528082.1
UniProt: A0A0H3HYA9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHGTYNLWLV ALSLAVATLA SYTALDLSGR IALLAHGRLR HAWLAGSAVA MGIGIGSMHV IGVLSVTLPI RIGFDAAITG LSLALAIVVS GFALYTIAGA QLTRLRLGIG GVLMGLGIAG MHYTGDAAMR MKPAIEYDPL LFTASIVIAL VASTVALWIA QTLIGIGRSR LARIGAAGVM GAAITGMHYT AYAAAIFAPG STCGAATGID LRWLAATVAL FTVAILIVAL ILSHFDVRTT LLAGSVSRLS GQIVRMAAFD TLTDLPNRHT LNEHLGRALA AARARGGKLA ILFMDLDGFK TINDSLGHPA GDEVLKTFSQ RLLRCVRDGD IVARLGGDEF VVLLESVSSD DEAARVARDV LERMRKGRWN DGQALQVTPS IGIALYPKDG DTADTLLRHA DAAMYAAKRG GRCTYRFFEA TMDEAAARAL KLQHALHEAL DSDYFSLCFQ PKFRCDGHRL AGAEALLRLN HPTLGKLAPM EFIPVAERSG QIIEIGYWVI RETCRHLRRW RSAHRAACRI AINLSPRQLA QPDLVANILE IVRAEHVEPR QLMFEITESV AMEDPPRTIE MIRLFQAHGF EIAIDDFGTG YSSLAYLQRF GAKQLKIDRF FTHALDEQGH EGGAIVSAII ALAHSLAMDV VAEGVETRSQ LDRLKTLMCD EIQGFLLGRP VPPEEFEAMV PLHSEAPA
NT Sequence
atgcacggca cctacaacct ttggctggtc gcgctctcgc ttgcggtcgc cacgctcgcc tcctataccg cgctcgacct gtcgggccgg atcgcgctgc tcgcgcacgg ccggctgcgc catgcgtggc tcgccggcag cgcggtcgcg atgggtatcg gcatcgggtc gatgcacgtc atcggcgtgc tgtcggtgac gctgcccatc cgcatcggct tcgacgccgc gatcaccggc ctgtcgctcg cgctcgcgat cgtggtgtcc ggcttcgcgc tgtatacgat cgccggcgcc caactgacga ggctgcggct cggcatcggc ggcgttctga tggggctcgg catcgcgggc atgcattaca ccggcgacgc cgcgatgcgg atgaagcccg ccatcgagta cgacccgctg ctgttcacgg catcgatcgt catcgcgctc gtcgcctcga cggtcgcgct atggatcgcg cagacgctca tcggcatcgg ccgatcgcgg ctggcccgca tcggcgcggc cggcgtgatg ggcgcggcga tcaccggcat gcactacacc gcctacgccg ccgcgatctt cgcccccggc tcgacctgcg gcgcggcgac cggcatcgat ctgcgctggc tcgcggcgac cgtcgcgctc ttcacggtcg cgatcctgat cgtcgcgctg atcctgtcgc acttcgacgt gcgcacgacg ctgctcgccg gatcggtttc gcgcctgagc ggccagatcg tgcgcatggc cgcattcgac acgctcacgg atctgccgaa ccgccacacg ctgaacgagc acctcggccg cgcgctcgcg gcggcgcggg cgcgcggcgg caagctggcg atcctgttca tggatctcga cggattcaag accatcaacg attcgctcgg ccacccggcc ggcgacgaag tgctgaaaac cttctcgcag cggctgctgc gctgcgtgcg agacggcgac atcgtcgcgc gcctcggcgg cgacgaattc gtcgtgctgc tggagagcgt ttcgtcggac gatgaagcgg cccgcgtcgc gcgcgatgtc ctcgaacgga tgcgcaaggg gcgctggaac gacgggcagg cgctacaggt cacgccgagc atcggcatcg cgctgtatcc gaaggacggc gatacggccg acacgctgct caggcacgcc gatgcggcga tgtatgcggc caagcgcggc ggccgctgca cctaccgctt cttcgaggcg acgatggacg aggcggcggc gcgggcgctc aagcttcagc acgcgctgca cgaagcgctc gattccgact acttctcgct gtgcttccag ccgaaattcc gctgcgacgg ccaccggctc gcgggcgcgg aagcgctgtt gcgcctgaat catccgacgc tcggcaaact cgcgccgatg gaattcatcc ccgtggccga acgctccggc cagatcatcg agatcggcta ctgggtgatc cgcgaaacgt gccggcacct cagacgctgg cggtccgcgc acagggcggc ctgccgcatc gcgatcaatc tgtcgcctcg gcagctcgcg cagccggatc tcgtcgcgaa catcctcgag atcgtgcgcg ccgagcacgt cgagccgcgc cagttgatgt tcgagatcac cgaatcggtc gcgatggaag acccgccgcg cacgatcgag atgatccggc tgttccaggc gcacggcttc gagatcgcga tcgacgactt cggcaccggc tattcgagcc tcgcctatct gcagcgattc ggcgcgaagc agctcaagat cgaccgattc ttcacccacg cgctcgacga gcaaggccac gaaggcggcg cgatcgtctc cgcgatcatc gcgctcgccc actcgctcgc gatggacgtg gtcgccgaag gcgtcgagac gcgctcgcag ctcgaccggc tcaagacgct gatgtgcgat gaaatccaag ggttcctgct cggccgcccc gtgccgccgg aagagttcga ggcgatggtg ccgctgcact ccgaggcgcc ggcatga
Details for BupsM.17236.d.B2.GE38810
HARVESTED ON: 1/22/2015
SEQUENCED ON: 1/15/2015
EXPECTED MW: 52kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMS HFDVRTTLLA GSVSRLSGQI VRMAAFDTLT DLPNRHTLNE HLGRALAAAR ARGGKLAILF MDLDGFKTIN DSLGHPAGDE VLKTFSQRLL RCVRDGDIVA RLGGDEFVVL LESVSSDDEA ARVARDVLER MRKGRWNDGQ ALQVTPSIGI ALYPKDGDTA DTLLRHADAA MYAAKRGGRC TYRFFEATMD EAAARALKLQ HALHEALDSD YFSLCFQPKF RCDGHRLAGA EALLRLNHPT LGKLAPMEFI PVAERSGQII EIGYWVIRET CRHLRRWRSA HRAACRIAIN LSPRQLAQPD LVANILEIVR AEHVEPRQLM FEITESVAME DPPRTIEMIR LFQAHGFEIA IDDFGTGYSS LAYLQRFGAK QLKIDRFFTH ALDEQGHEGG AIVSAIIALA HSLAMDVVAE GVETRSQLDR LKTLMCDEIQ GFLLGRPVPP EEFEAMVP
Validated NT Sequence
atggctcacc accaccacca ccatatgtcg cacttcgacg tgcgcacgac gctgctcgcc ggatcggttt cgcgcctgag cggccagatc gtgcgcatgg ccgcattcga cacgctcacg gatctgccga accgccacac gctgaacgag cacctcggcc gcgcgctcgc ggcggcgcgg gcgcgcggcg gcaagctggc gatcctgttc atggatctcg acggattcaa gaccatcaac gattcgctcg gccacccggc cggcgacgaa gtgctgaaaa ccttctcgca gcggctgctg cgctgcgtgc gagacggcga catcgtcgcg cgcctcggcg gcgacgaatt cgtcgtgctg ctggagagcg tttcgtcgga cgatgaagcg gcccgcgtcg cgcgcgatgt cctcgaacgg atgcgcaagg ggcgctggaa cgacgggcag gcgctacagg tcacgccgag catcggcatc gcgctgtatc cgaaggacgg cgatacggcc gacacgctgc tcaggcacgc cgatgcggcg atgtatgcgg ccaagcgcgg cggccgctgc acctaccgct tcttcgaggc gacgatggac gaggcggcgg cgcgggcgct caagcttcag cacgcgctgc acgaagcgct cgattccgac tacttctcgc tgtgcttcca gccgaaattc cgctgcgacg gccaccggct cgcgggcgcg gaagcgctgt tgcgcctgaa tcatccgacg ctcggcaaac tcgcgccgat ggaattcatc cccgtggccg aacgctccgg ccagatcatc gagatcggct actgggtgat ccgcgaaacg tgccggcacc tcagacgctg gcggtccgcg cacagggcgg cctgccgcat cgcgatcaat ctgtcgcctc ggcagctcgc gcagccggat ctcgtcgcga acatcctcga gatcgtgcgc gccgagcacg tcgagccgcg ccagttgatg ttcgagatca ccgaatcggt cgcgatggaa gacccgccgc gcacgatcga gatgatccgg ctgttccagg cgcacggctt cgagatcgcg atcgacgact tcggcaccgg ctattcgagc ctcgcctatc tgcagcgatt cggcgcgaag cagctcaaga tcgaccgatt cttcacccac gcgctcgacg agcaaggcca cgaaggcggc gcgatcgtct ccgcgatcat cgcgctcgcc cactcgctcg cgatggacgt ggtcgccgaa ggcgtcgaga cgcgctcgca gctcgaccgg ctcaagacgc tgatgtgcga tgaaatccaa gggttcctgc tcggccgccc cgtgccgccg gaagagttcg aggcgatggt gccga
Expected Protein Sequence
MAHHHHHHSH FDVRTTLLAG SVSRLSGQIV RMAAFDTLTD LPNRHTLNEH LGRALAAARA RGGKLAILFM DLDGFKTIND SLGHPAGDEV LKTFSQRLLR CVRDGDIVAR LGGDEFVVLL ESVSSDDEAA RVARDVLERM RKGRWNDGQA LQVTPSIGIA LYPKDGDTAD TLLRHADAAM YAAKRGGRCT YRFFEATMDE AAARALKLQH ALHEALDSDY FSLCFQPKFR CDGHRLAGAE ALLRLNHPTL GKLAPMEFIP VAERSGQIIE IGYWVIRETC RHLRRWRSAH RAACRIAINL SPRQLAQPDL VANILEIVRA EHVEPRQLMF EITESVAMED PPRTIEMIRL FQAHGFEIAI DDFGTGYSSL AYLQRFGAKQ LKIDRFFTHA LDEQGHEGGA IVSAIIALAH SLAMDVVAEG VETRSQLDRL KTLMCDEIQG FLLGRPVPPE EFEAMVPLHS EAPA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcgcact tcgacgtgcg cacgacgctg ctcgccggat cggtttcgcg cctgagcggc cagatcgtgc gcatggccgc attcgacacg ctcacggatc tgccgaaccg ccacacgctg aacgagcacc tcggccgcgc gctcgcggcg gcgcgggcgc gcggcggcaa gctggcgatc ctgttcatgg atctcgacgg attcaagacc atcaacgatt cgctcggcca cccggccggc gacgaagtgc tgaaaacctt ctcgcagcgg ctgctgcgct gcgtgcgaga cggcgacatc gtcgcgcgcc tcggcggcga cgaattcgtc gtgctgctgg agagcgtttc gtcggacgat gaagcggccc gcgtcgcgcg cgatgtcctc gaacggatgc gcaaggggcg ctggaacgac gggcaggcgc tacaggtcac gccgagcatc ggcatcgcgc tgtatccgaa ggacggcgat acggccgaca cgctgctcag gcacgccgat gcggcgatgt atgcggccaa gcgcggcggc cgctgcacct accgcttctt cgaggcgacg atggacgagg cggcggcgcg ggcgctcaag cttcagcacg cgctgcacga agcgctcgat tccgactact tctcgctgtg cttccagccg aaattccgct gcgacggcca ccggctcgcg ggcgcggaag cgctgttgcg cctgaatcat ccgacgctcg gcaaactcgc gccgatggaa ttcatccccg tggccgaacg ctccggccag atcatcgaga tcggctactg ggtgatccgc gaaacgtgcc ggcacctcag acgctggcgg tccgcgcaca gggcggcctg ccgcatcgcg atcaatctgt cgcctcggca gctcgcgcag ccggatctcg tcgcgaacat cctcgagatc gtgcgcgccg agcacgtcga gccgcgccag ttgatgttcg agatcaccga atcggtcgcg atggaagacc cgccgcgcac gatcgagatg atccggctgt tccaggcgca cggcttcgag atcgcgatcg acgacttcgg caccggctat tcgagcctcg cctatctgca gcgattcggc gcgaagcagc tcaagatcga ccgattcttc acccacgcgc tcgacgagca aggccacgaa ggcggcgcga tcgtctccgc gatcatcgcg ctcgcccact cgctcgcgat ggacgtggtc gccgaaggcg tcgagacgcg ctcgcagctc gaccggctca agacgctgat gtgcgatgaa atccaagggt tcctgctcgg ccgccccgtg ccgccggaag agttcgaggc gatggtgccg ctgcactccg aggcgccggc atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat