BupsM.17236.c

Sensory box protein

CENTER ID:	BupsM.17236.c
ORGANISM:	Burkholderia pseudomallei 1026b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsM.17236.c.B3.GE38809	soluble domain #2		235	807

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|884204.6.peg.7142
RefSeq:	YP_006279105.1
UniProt:	A0A0H3HXS7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MREQALGMND GGGQAAELRP ALDVWPILLM VSVLVCVVIV SLAVVSFLRV YIGGESTWSK SQKDAVIYLI RYAETGDERA YKLYEAAIDK PYRLSLARAA LQRKPPDRAS AREAIIASGI DPTDATAAAL LLPALSWVAK INYALQCWMQ ADVELAEFRS VASQLHALVS SGHRDDARVK ALEYRAWEIN ERVSGLPDRF SKAFSDEFRS SVALLLVCYF VASMFLMYLA LVWARKAALQ RLSIQYALDR SEAFAEAALS SVAEAVITVG LAKNVDFINN AAEQLLGYPA AACVGAPVSS VLMLLDKETG HAVDIVDEFW AREERTPIKR EVHLLRRDHS KIVVQTTISE MGDRVHGHIG YVLILRNMTR EQQFLESLAW QATHDVLTGL VNRVEFERRL EIALADTASG KERADSMLLM LDLDRFKEIN DTCGHAAGDA MLREVTQRFQ SCLDDDDVLA RLGGDEFGVL LPHGAGSWPS KNKAERLRRS LEDFVFLWEG ERFTVSVSIG VLELCKAPRN LEMAVKLADI ACYIAKERGR NRIQLADPSD LQQARHVGDV QWSRRIKTAL ENDGFRLYVQ PIVHTRPDSR TPERAEVLLR MMDSAGRDIS PAAFLPAAER YGLMGLIDRW VIRTVFRKLS ALPAREYHEY NVNLSGASIS DERFLEFVIA ELLSSGLEPS VICFEITETI AVKNLELASR FMGELRTIGC RFALDDFGAG MSSFRYLRSL PIDYLKIDGE FVSNMMSDRV SYGVVSAINE VAHSMRCSTV AEHVESEVEL AMLRELGIDF CQGYFFAQPS PWREGGY

NT Sequence

atgcgcgaac aggcgctcgg catgaacgac gggggtggcc aggcggccga actgaggccg gcgctcgacg tctggccgat cctgctgatg gtgtcggtgc tcgtgtgcgt cgtgatcgtg agcctcgcgg tggtgtcgtt cctcagggtc tacatcggcg gcgagagcac gtggtcgaag tcgcagaagg acgcggtcat ctacctgatc cgctatgcgg agacgggcga cgagcgcgcg tacaagctct atgaggccgc gatcgacaag ccgtatcggc tgagtctcgc gcgcgccgcg ctgcagcgca agccgcccga tcgcgcgagc gcgcgcgagg cgatcatcgc gagcggcatc gatccgaccg acgcgaccgc ggccgcgctg ctgctgcccg cgctcagctg ggtcgcgaag atcaactatg cgctgcaatg ctggatgcag gcggacgtcg agctcgccga gttccgcagc gtcgcgagcc agcttcatgc gctcgtcagc agcggccatc gcgacgacgc ccgcgtgaag gcgctcgaat accgcgcgtg ggaaatcaac gaacgcgtgt ccggcctgcc cgatcgcttc tcgaaggcgt tcagcgacga attccgttcg agcgtcgcgc tgctgctcgt ctgctacttc gtcgcgtcga tgttcctgat gtatctcgcg ctcgtctggg cgcgcaaggc cgcgctgcag cggctgtcga tccagtacgc gctcgatcgc agcgaggcgt tcgccgaggc ggcgctgagc tcggtcgcgg aggcggtgat cacggtcggg ctcgcgaaga acgtcgactt catcaacaac gcggccgagc agctgctcgg ctacccggcc gccgcgtgcg tcggcgcgcc ggtgtcgagc gtgctgatgc tgctcgacaa ggagacgggg cacgcggtcg acatcgtcga cgaattctgg gcgcgcgagg agcgcacgcc gatcaagcgc gaggtgcatc tgctgcgccg cgatcattcg aagatcgttg tgcagacaac gatttcggag atgggcgacc gcgtgcacgg gcacatcggc tacgtgctga tcctgcgcaa catgacgcgc gagcagcagt tcctcgaaag cctcgcgtgg caggcgacgc acgacgtgct gacggggctc gtgaaccgcg tcgagttcga gcgccggctc gagatcgcgc ttgccgacac ggcctccggc aaggagcgcg ccgattcgat gctgctgatg ctcgatctcg accgcttcaa ggagatcaac gacacctgcg gccacgcggc gggcgacgcg atgctgcgcg aagtcacgca gcgcttccag agctgcctcg acgacgacga cgtgctcgcg cggctcggcg gcgacgagtt cggcgtgctg ctgccgcacg gcgcgggatc gtggccttcg aagaacaagg cggagcggct gcgccgcagc ctcgaggatt tcgtgttcct gtgggagggc gagcgcttca cggtgagcgt gagcatcggc gtgctcgagc tctgcaaggc gccgcgcaat ctggagatgg ccgtcaagct cgccgacatc gcgtgctaca tcgcgaagga gcgcgggcgc aaccgcatcc agctcgcgga cccgagcgat ctgcaacagg cgcgccacgt cggcgacgtg caatggagcc gccggatcaa gaccgcgctc gaaaacgacg gctttcgcct gtacgtgcag ccgatcgtcc atacgcgccc ggattcgcgg acgcccgagc gcgcggaggt gctgctgcgc atgatggaca gcgcgggccg cgacatctcg cccgccgcgt tcctgccggc cgccgagcgc tacgggctga tggggctcat cgaccgctgg gtgatccgca cggtgttccg caagctgagc gcgctacccg cgcgcgagta ccacgagtac aacgtgaatc tgtcgggggc gtcgatctcc gacgagcgct ttctcgaatt cgtgatcgcc gagctgctga gctccggtct cgagccttcg gtgatctgtt tcgagatcac cgagacgatc gccgtgaaga acctcgagct cgcgtcgcgc ttcatgggcg agctgcgcac gatcggctgc cgcttcgcgc tcgacgattt cggcgcgggc atgtcgtcgt ttcgctatct gcgcagcctg ccgatcgatt atctgaagat cgacggcgag ttcgtgtcga acatgatgtc ggaccgggtg agttacggcg tcgtcagcgc gatcaacgaa gtcgcgcact cgatgcgttg cagcaccgtc gccgagcatg tcgagtccga ggtcgagctc gcgatgctgc gcgagctcgg catcgatttc tgccagggat atttctttgc tcagccttcg ccgtggcgcg agggcggcta ttga

Details for BupsM.17236.c.B3.GE38809

HARVESTED ON:	1/22/2015
SEQUENCED ON:	1/15/2015
EXPECTED MW:	65kDa
OBSERVED MW:	65kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	97
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMR KAALQRLSIQ YALDRSEAFA EAALSSVAEA VITVGLAKNV DFINNAAEQL LGYPAAACVG APVSSVLMLL DKETGHAVDI VDEFWAREER TPIKREVHLL RRDHSKIVVQ TTISEMGDRV HGHIGYVLIL RNMTREQQFL ESLAWQATHD VLTGLVNRVE FERRLEIALA DTASGKERAD SMLLMLDLDR FKEINDTCGH AAGDAMLREV TQRFQSCLDD XXVLARLGGD EFGVLLPXXA GSWPSKXXXE RLXXXXPDFV FLWEGERFTV SVSIGVLELC KAPRNLEMAV KLADIACXIA KERXXNRIQL ADPSDLQQAR HVGDVQWSRR IKTALENDGF RLYVQPIVHT RPDSRTPERA EVLLRMMDSA GRDISPAAFL PAAERYGLMG LIDRWVIRTV FRKLSALPAR EYHEYNVNLS GASISDERFL EFVIAELLSS GLEPSVICFE ITETIAVKNL ELASRFMGEL RTIGCRFALD DFGAGMSSFR YLRSLPIDYL KIDGEFVSNM MSDRVSYGVV SAINEVAHSM RCSTVAEHVE SEVELAMLRE LGIDFCQGYF FAQP

Validated NT Sequence

atggctcacc accaccacca ccatatgcgc aaggccgcgc tgcagcggct gtcgatccag tacgcgctcg atcgcagcga ggcgttcgcc gaggcggcgc tgagctcggt cgcggaggcg gtgatcacgg tcgggctcgc gaagaacgtc gacttcatca acaacgcggc cgagcagctg ctcggctacc cggccgccgc gtgcgtcggc gcgccggtgt cgagcgtgct gatgctgctc gacaaggaga cggggcacgc ggtcgacatc gtcgacgaat tctgggcgcg cgaggagcgc acgccgatca agcgcgaggt gcatctgctg cgccgcgatc attcgaagat cgttgtgcag acaacgattt cggagatggg cgaccgcgtg cacgggcaca tcggctacgt gctgatcctg cgcaacatga cgcgcgagca gcagttcctc gaaagcctcg cgtggcaggc gacgcacgac gtgctgacgg ggctcgtgaa ccgcgtcgag ttcgagcgcc ggctcgagat cgcgcttgcc gacacggcct ccggcaagga gcgcgccgat tcgatgctgc tgatgctcga tctcgaccgc ttcaaggaga tcaacgacac ctgcggccac gcggcgggcg acgcgatgct gcgcgaagtc acgcagcgct tccagagctg cctcgacgac gnnnncgtgc tcgcgcggct cggcggcgac gagttcggcg tgctgctgcc gcanngcgcg ggatcgtggc cttcgaagaa nnnnnnggag cggctnnnnn ncnnnnnncc agatttcgtg ttcctgtggg agggcgagcg cttcacggtg agcgtgagca tcggcgtgct cgagctctgc aaggcgccgc gcaatctgga gatggccgtc aagctcgccg acatcgcgtg ctanatcgcg aaggagcgnn nnngcaaccg catccagctc gcggacccga gcgatctgca acaggcgcgc cacgtcggcg acgtgcaatg gagccgccgg atcaagaccg cgctcgaaaa cgacggcttt cgcctgtacg tgcagccgat cgtccatacg cgcccggatt cgcggacgcc cgagcgcgcg gaggtgctgc tgcgcatgat ggacagcgcg ggccgcgaca tctcgcccgc cgcgttcctg ccggccgccg agcgctacgg gctgatgggg ctcatcgacc gctgggtgat ccgcacggtg ttccgcaagc tgagcgcgct acccgcgcgc gagtaccacg agtacaacgt gaatctgtcg ggggcgtcga tctccgacga gcgctttctc gaattcgtga tcgccgagct gctgagctcc ggtctcgagc cttcggtgat ctgtttcgag atcaccgaga cgatcgccgt gaagaacctc gagctcgcgt cgcgcttcat gggcgagctg cgcacgatcg gctgccgctt cgcgctcgac gatttcggcg cgggcatgtc gtcgtttcgc tatctgcgca gcctgccgat cgattatctg aagatcgacg gcgagttcgt gtcgaacatg atgtcggacc gggtgagtta cggcgtcgtc agcgcgatca acgaagtcgc gcactcgatg cgttgcagca ccgtcgccga gcatgtcgag tccgaggtcg agctcgcgat gctgcgcgag ctcggcatcg atttctgcca gggatatttc tttgctcagc cttc

Expected Protein Sequence

MAHHHHHHRK AALQRLSIQY ALDRSEAFAE AALSSVAEAV ITVGLAKNVD FINNAAEQLL GYPAAACVGA PVSSVLMLLD KETGHAVDIV DEFWAREERT PIKREVHLLR RDHSKIVVQT TISEMGDRVH GHIGYVLILR NMTREQQFLE SLAWQATHDV LTGLVNRVEF ERRLEIALAD TASGKERADS MLLMLDLDRF KEINDTCGHA AGDAMLREVT QRFQSCLDDD DVLARLGGDE FGVLLPHGAG SWPSKNKAER LRRSLEDFVF LWEGERFTVS VSIGVLELCK APRNLEMAVK LADIACYIAK ERGRNRIQLA DPSDLQQARH VGDVQWSRRI KTALENDGFR LYVQPIVHTR PDSRTPERAE VLLRMMDSAG RDISPAAFLP AAERYGLMGL IDRWVIRTVF RKLSALPARE YHEYNVNLSG ASISDERFLE FVIAELLSSG LEPSVICFEI TETIAVKNLE LASRFMGELR TIGCRFALDD FGAGMSSFRY LRSLPIDYLK IDGEFVSNMM SDRVSYGVVS AINEVAHSMR CSTVAEHVES EVELAMLREL GIDFCQGYFF AQPSPWREGG Y

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcgcaagg ccgcgctgca gcggctgtcg atccagtacg cgctcgatcg cagcgaggcg ttcgccgagg cggcgctgag ctcggtcgcg gaggcggtga tcacggtcgg gctcgcgaag aacgtcgact tcatcaacaa cgcggccgag cagctgctcg gctacccggc cgccgcgtgc gtcggcgcgc cggtgtcgag cgtgctgatg ctgctcgaca aggagacggg gcacgcggtc gacatcgtcg acgaattctg ggcgcgcgag gagcgcacgc cgatcaagcg cgaggtgcat ctgctgcgcc gcgatcattc gaagatcgtt gtgcagacaa cgatttcgga gatgggcgac cgcgtgcacg ggcacatcgg ctacgtgctg atcctgcgca acatgacgcg cgagcagcag ttcctcgaaa gcctcgcgtg gcaggcgacg cacgacgtgc tgacggggct cgtgaaccgc gtcgagttcg agcgccggct cgagatcgcg cttgccgaca cggcctccgg caaggagcgc gccgattcga tgctgctgat gctcgatctc gaccgcttca aggagatcaa cgacacctgc ggccacgcgg cgggcgacgc gatgctgcgc gaagtcacgc agcgcttcca gagctgcctc gacgacgacg acgtgctcgc gcggctcggc ggcgacgagt tcggcgtgct gctgccgcac ggcgcgggat cgtggccttc gaagaacaag gcggagcggc tgcgccgcag cctcgaggat ttcgtgttcc tgtgggaggg cgagcgcttc acggtgagcg tgagcatcgg cgtgctcgag ctctgcaagg cgccgcgcaa tctggagatg gccgtcaagc tcgccgacat cgcgtgctac atcgcgaagg agcgcgggcg caaccgcatc cagctcgcgg acccgagcga tctgcaacag gcgcgccacg tcggcgacgt gcaatggagc cgccggatca agaccgcgct cgaaaacgac ggctttcgcc tgtacgtgca gccgatcgtc catacgcgcc cggattcgcg gacgcccgag cgcgcggagg tgctgctgcg catgatggac agcgcgggcc gcgacatctc gcccgccgcg ttcctgccgg ccgccgagcg ctacgggctg atggggctca tcgaccgctg ggtgatccgc acggtgttcc gcaagctgag cgcgctaccc gcgcgcgagt accacgagta caacgtgaat ctgtcggggg cgtcgatctc cgacgagcgc tttctcgaat tcgtgatcgc cgagctgctg agctccggtc tcgagccttc ggtgatctgt ttcgagatca ccgagacgat cgccgtgaag aacctcgagc tcgcgtcgcg cttcatgggc gagctgcgca cgatcggctg ccgcttcgcg ctcgacgatt tcggcgcggg catgtcgtcg tttcgctatc tgcgcagcct gccgatcgat tatctgaaga tcgacggcga gttcgtgtcg aacatgatgt cggaccgggt gagttacggc gtcgtcagcg cgatcaacga agtcgcgcac tcgatgcgtt gcagcaccgt cgccgagcat gtcgagtccg aggtcgagct cgcgatgctg cgcgagctcg gcatcgattt ctgccaggga tatttctttg ctcagccttc gccgtggcgc gagggcggct attgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat