SSGCID: Target Page

BupsM.17236.b

EAL domain-containing protein

CENTER ID:	BupsM.17236.b
ORGANISM:	Burkholderia pseudomallei 1026b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsM.17236.b.B1.GE38807	full length		1	248

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|884204.3.peg.1756
RefSeq:	YP_006274606.1
RefSeq:	WP_004550403.1
UniProt:	A0A0H3HPY2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDYQPIVSIR SGAVTGAEAT IRWDHPEWGA LPQRVVHAAA DRLGAASRIA GHAFGEACRQ LARWKQQGAG VSALSMRLSG AQLGAESVFE RLALSIEAFD ISSSQFTLEV PEMSAPEESL SLLDRLKRLR QKGYGIVLGD FGAHHTAMST LMVLPVTGVK FGESFTERLP GSPTAEAILS SVSRLAHDLG FTLTVSGVEN GRQFELLRRF RDIELQGAFL FEPMRAEVWH ERVNPRALSQ LNLREPPF

NT Sequence

atggattatc agccgatcgt ctcgatccgc tccggggccg tgacgggcgc cgaagcgacg atacgatggg atcatccgga atggggcgcg ctgcctcagc gtgtggtcca cgccgccgcg gaccgattgg gcgcggcgtc ccggatcgcc ggccacgcgt tcggcgaggc gtgcaggcag ctcgcgcgct ggaagcagca gggcgccggc gtgtcggcgc tgtcgatgcg gctttcgggc gcccagttgg gcgccgaaag cgtattcgag cgactcgcgc tgtcgatcga ggcgttcgat atctcgagca gccagttcac gctcgaagtg ccggaaatgt cggcgccgga ggaatcgctg agtcttctcg atcgcctgaa gcggctgagg cagaagggtt acggcatcgt gctcggcgat ttcggagcgc atcacacggc gatgtcgacg ttgatggtgc tgcccgtcac gggcgtcaaa ttcggcgaat cgttcacgga gcgattgccc ggttcgccga ctgcggaggc gatcctgtcg agcgtctcgc gtctcgcgca cgatctgggt ttcacgttga cggtgtccgg cgtcgaaaac ggccggcagt tcgaattgct gcgtcgcttt cgcgatatcg agctgcaggg cgcctttctg ttcgagccga tgcgcgccga ggtgtggcac gagcgcgtga atccgagagc gctgtcgcaa ctgaatctgc gtgagccgcc gttctga

Details for BupsM.17236.b.B1.GE38807

HARVESTED ON:	1/22/2015
SEQUENCED ON:	1/15/2015
EXPECTED MW:	28kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMD YQPIVSIRSG AVTGAEATIR WDHPEWGALP QRVVHAAADR LGAASRIAGH AFGEACRQLA RWKQQGAGVS ALSMRLSGAQ LGAESVFERL ALSIEAFDIS SSQFTLEVPE MSAPEESLSL LDRLKRLRQK GYGIVLGDFG AHHTAMSTLM VLPVTGVKFG ESFTERLPGS PTAEAILSSV SRLAHDLGFT LTVSGVENGR QFELLRRFRD IELQGAFLFE PMRAEVWHER VNPRALSQLN LREPPF

Validated NT Sequence

atggctcacc accaccacca ccatatggat tatcagccga tcgtctcgat ccgctccggg gccgtgacgg gcgccgaagc gacgatacga tgggatcatc cggaatgggg cgcgctgcct cagcgtgtgg tccacgccgc cgcggaccga ttgggcgcgg cgtcccggat cgccggccac gcgttcggcg aggcgtgcag gcagctcgcg cgctggaagc agcagggcgc cggcgtgtcg gcgctgtcga tgcggctttc gggcgcccag ttgggcgccg aaagcgtatt cgagcgactc gcgctgtcga tcgaggcgtt cgatatctcg agcagccagt tcacgctcga agtgccggaa atgtcggcgc cggaggaatc gctgagtctt ctcgatcgcc tgaagcggct gaggcagaag ggttacggca tcgtgctcgg cgatttcgga gcgcatcaca cggcgatgtc gacgttgatg gtgctgcccg tcacgggcgt caaattcggc gaatcgttca cggagcgatt gcccggttcg ccgactgcgg aggcgatcct gtcgagcgtc tcgcgtctcg cgcacgatct gggtttcacg ttgacggtgt ccggcgtcga aaacggccgg cagttcgaat tgctgcgtcg ctttcgcgat atcgagctgc agggcgcctt tctgttcgag ccgatgcgcg ccgaggtgtg gcacgagcgc gtgaatccga gagcgctgtc gcaactgaat ctgcgtgagc cgccgttcta agtgagtaag at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatt atcagccgat cgtctcgatc cgctccgggg ccgtgacggg cgccgaagcg acgatacgat gggatcatcc ggaatggggc gcgctgcctc agcgtgtggt ccacgccgcc gcggaccgat tgggcgcggc gtcccggatc gccggccacg cgttcggcga ggcgtgcagg cagctcgcgc gctggaagca gcagggcgcc ggcgtgtcgg cgctgtcgat gcggctttcg ggcgcccagt tgggcgccga aagcgtattc gagcgactcg cgctgtcgat cgaggcgttc gatatctcga gcagccagtt cacgctcgaa gtgccggaaa tgtcggcgcc ggaggaatcg ctgagtcttc tcgatcgcct gaagcggctg aggcagaagg gttacggcat cgtgctcggc gatttcggag cgcatcacac ggcgatgtcg acgttgatgg tgctgcccgt cacgggcgtc aaattcggcg aatcgttcac ggagcgattg cccggttcgc cgactgcgga ggcgatcctg tcgagcgtct cgcgtctcgc gcacgatctg ggtttcacgt tgacggtgtc cggcgtcgaa aacggccggc agttcgaatt gctgcgtcgc tttcgcgata tcgagctgca gggcgccttt ctgttcgagc cgatgcgcgc cgaggtgtgg cacgagcgcg tgaatccgag agcgctgtcg caactgaatc tgcgtgagcc gccgttctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at