BupsM.17236.a

EAL/GGDEF domain-containing protein

CENTER ID: BupsM.17236.a
ORGANISM: Burkholderia pseudomallei 1026b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsM.17236.a.B4.GE38806 PAS domain 181 333
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|884204.3.peg.3224
RefSeq: YP_006275912.1
UniProt: A0A0H3HMH0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLGAALLSVE RFGAARSRAS AAIPASIGAR LSGCAFIAVR IGAALSRRAP SPAHAAAPGV ARPDFDAIER VFGPIRMRER RAHGATCTAD APDASHAGWI EPARACSAAA HAAPGRGAEG APNTMPGPSE AGAAAVPQSQ PYLDMIDSTF REPAAIVLAL SLLGMIALAL LPVFRIRRLA LRLGEAQGAL EMSEARARAA LVAVGDGVIF TGRAGRVECL NPAAERLIGM LADDCRGRPL VSTLRLSRTA TSASSGAFAA SAGLDAPDSP GSAASPDAPL GDLEDGGSCD ATLYRADGGA IAVRATASSI APPPGHARRA CGSGRVLVLK SLATEHELVR RLAWQTTHDP LTGLASRAEF ERHVGFALAA DVREPVALLF VDLDRFRIVN DTCGYAAGDA MLAALAARLV SCAASADVVA RLGGDEFCVL LDARDEASAV SAAERLRASV DGFVFVWDGQ PFSVTASVGV ALLGGPGRAP RVEDAVRLAG IACDVAKARG RNRVQLADPH DHELAHHISD VSWCARVRQA LEYDDFRLYV QPIVDTATQG ATGLPRARRG ELLLRMGALG EREGVAPPGL FIRAAERYGL VTDIDRWVVR TVLDALARTR SRRFSEYAIN LSGISIGDER FLDYVLEQFA RTRVAPALIC FEITETAAIA NLAGALRFMH ELKALGCRFA LDDFGSGMAS LSYLKQLPVE YLKIDGSFVT GIANDAASLD IVASINDIGH AMNCKTIAEY VDSAATLQKL AALGVDYAQG YYIGRPVPWC EAARA
NT Sequence
ttgctcggcg cggcgctgct gtccgtcgag cggttcggcg ccgcgcgaag ccgcgcgtcg gccgcgatcc cggcgagcat cggcgcgcgc ctgagcggct gcgcgttcat cgccgtgcgc atcggcgcgg cgctgtcgcg gcgcgcgccg tcgccggcgc atgcggcggc gcctggcgtt gcgcggccgg atttcgacgc gatcgaacgc gtgttcggcc cgatccgcat gcgcgagcgg cgcgcacatg gcgcgacgtg caccgcggac gcgccggacg cgtcgcacgc agggtggatc gaacccgcgc gcgcgtgctc ggccgccgcg catgcggcgc cggggcgcgg cgccgagggc gcgccgaaca cgatgccggg gccgtccgaa gcgggcgccg cggcggtccc acaatcgcaa ccgtacctgg acatgatcga ttcgacgttt cgcgaaccgg cggccatcgt gctcgctttg agcctgctcg gcatgatcgc gctcgcactg ctgcccgtgt tccggatcag gcggctcgcg cttcgcctcg gcgaggcgca gggagcgctc gagatgagcg aagcgcgcgc ccgcgcggcg ctcgtcgccg tcggcgacgg cgtgatcttc accggccggg cggggcgcgt cgaatgcctg aatccggcgg cggagcgatt gatcggcatg ctcgccgacg attgccgcgg ccgtccgctc gtgtcgacgc tgcggctgtc gcgcacggcc acgtccgctt cgtccggcgc gttcgccgcg tccgccgggc tcgacgcgcc ggattcgccg ggctcggccg cctcgcccga tgcgccgctc ggcgatctcg aggacggcgg cagttgcgac gcgacgctct accgcgcgga cggcggcgcg atcgccgtgc gcgcgaccgc gtcgtcgatc gcgccgccgc cggggcatgc gcggcgcgcg tgcggcagcg gccgcgtgct cgtgctcaag agcctcgcga ccgagcacga gctcgtgcgc cgcctcgcgt ggcagacaac gcacgatccg ctcaccggcc tggcgagccg cgcagagttc gagcgtcacg ttggtttcgc gctcgccgcc gacgtgcgcg agccggtcgc gctgctgttc gtcgacctgg atcgcttccg gatcgtcaac gatacgtgcg gctacgcggc gggcgacgcg atgctcgccg cgctcgccgc gcgtctcgtg tcgtgcgcgg cgtcggccga cgtcgtcgcg cggctcggcg gcgacgaatt ctgcgtgctg ctcgacgccc gcgacgaagc gtcggccgtg agcgcggccg agcgcctgcg cgcgagcgtc gacggcttcg tgttcgtctg ggacggccag ccgttctcgg tgacggcgag cgtcggcgtc gcactgctcg gcgggccggg ccgcgcgccg cgcgtcgagg atgcggtgcg gctcgccggc atcgcgtgcg acgtcgcgaa ggcgcgcggg cgcaaccgcg tgcaactggc cgatccgcac gatcacgagc tcgcgcacca catcagcgac gtatcgtggt gcgcgcgcgt gaggcaggcg ctcgaatacg acgatttccg cctgtacgtg cagccgatcg tcgatacggc gacgcagggt gcgacgggcc tgccgcgcgc gaggcgcggc gagctgctgc tgcgcatggg cgcgctcggc gagcgcgagg gcgtcgcgcc gcccggcctt ttcatccgcg cggccgagcg ctacgggctc gtcaccgaca tcgaccgctg ggtcgtgcgc acggtgctcg acgcgctcgc gcgcacgcgc agccggcgct tcagcgaata cgcgatcaat ctgtcgggga tctcgatcgg cgacgagcgc tttctcgact atgtgctcga acagttcgcg cgcacgcgcg tcgcgcccgc gctcatctgt ttcgagatca ccgagacggc ggcgatcgcg aacctggccg gcgcgctgcg cttcatgcac gagctgaagg cgctcggctg ccggttcgcg ctcgacgatt tcggctccgg gatggcgtcg ctcagctatc tgaagcaact gcccgtcgag tacctgaaga tcgacggcag cttcgtgacg ggcatcgcga acgatgccgc gagcctcgac atcgtcgcgt cgatcaacga catcgggcac gcgatgaatt gcaagacgat cgccgagtac gtcgacagcg cggcgacgct gcaaaagctc gccgcgctcg gcgtcgatta tgcgcagggc tactacatcg gccggcccgt gccgtggtgc gaggccgcgc gcgcgtag
Details for BupsM.17236.a.B4.GE38806
HARVESTED ON: 1/22/2015
SEQUENCED ON: 1/15/2015
EXPECTED MW: 16kDa
OBSERVED MW: 19kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 79
Validated AA Sequence
MAHHHHHHML RLGEAQGALE MSEARARAAL VAVGDGVIFT GRAGRVECLN PAAERLIGML ADDCRGRPLV STLRLSRTAT SASSGAFAAS AGLDAPDSPG SAASPDAPLG DLEDGGSCDA TLYRADGX
Validated NT Sequence
atggctcacc accaccacca ccatatgctt cgcctcggcg aggcgcaggg agcgctcgag atgagcgaag cgcgcgcccg cgcggcgctc gtcgccgtcg gcgacggcgt gatcttcacc ggccgggcgg ggcgcgtcga atgcctgaat ccggcggcgg agcgattgat cggcatgctc gccgacgatt gccgcggccg tccgctcgtg tcgacgctgc ggctgtcgcg cacggccacg tccgcttcgt ccggcgcgtt cgccgcgtcc gccgggctcg acgcgccgga ttcgccgggc tcggccgcct cgcccgatgc gccgctcggc gatctcgagg acggcggcag ttgcgacgcg acgctctacc gcgcggacgg nnnnnnctgt atannnnnnn nntcccctat tgnnnnnnnn ntnnncgnnn nnnattnnnn nnnnacnnnn tcagacttaa ntnnnnaagt aaaganannn nnngatgttt taagggaann nnaagagnnn ngtaaacctc atnnnnnnnn tgannatatc actgtatann nnnnannata tatnnnnnna
Expected Protein Sequence
MAHHHHHHLR LGEAQGALEM SEARARAALV AVGDGVIFTG RAGRVECLNP AAERLIGMLA DDCRGRPLVS TLRLSRTATS ASSGAFAASA GLDAPDSPGS AASPDAPLGD LEDGGSCDAT LYRADGGAIA VRATASSIAP PPGHARRACG SGRVLVLKSL A
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcttcgcc tcggcgaggc gcagggagcg ctcgagatga gcgaagcgcg cgcccgcgcg gcgctcgtcg ccgtcggcga cggcgtgatc ttcaccggcc gggcggggcg cgtcgaatgc ctgaatccgg cggcggagcg attgatcggc atgctcgccg acgattgccg cggccgtccg ctcgtgtcga cgctgcggct gtcgcgcacg gccacgtccg cttcgtccgg cgcgttcgcc gcgtccgccg ggctcgacgc gccggattcg ccgggctcgg ccgcctcgcc cgatgcgccg ctcggcgatc tcgaggacgg cggcagttgc gacgcgacgc tctaccgcgc ggacggcggc gcgatcgccg tgcgcgcgac cgcgtcgtcg atcgcgccgc cgccggggca tgcgcggcgc gcgtgcggca gcggccgcgt gctcgtgctc aagagcctcg cgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat