SSGCID: Target Page

BupsM.10014.a

Lipoprotein-releasing system ATP-binding protein LolD (EC 3.6.3.-) (lolD BP1026B_I1054)

CENTER ID:	BupsM.10014.a
ORGANISM:	Burkholderia pseudomallei 1026b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsM.10014.a.B1.GE42238	full length		1	240

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|884204.3.peg.1160
UniProt:	A0A0H3HH26

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNQNHQDGGA RECVLEARGV TKTFVQGGFN VQVLDNAQVS VRRGEKLAIV GASGSGKSTL LHVLGGLDEP SAGQVSLLGK PFTQLAERER NELRNRALGF VYQFHHLLPE FTALDNVAMP LRIRRMSTEE ARRHAREMLE QVGLGPRAKH RPGELSGGER QRVAIARALV TKPACVLADE PTGNLDGSTA DHVFHLMLEL SRTLDTSFVI VTHDPDLAAR CDRILRLRDG VLHEEPAVPV

NT Sequence

atgaatcaga accatcagga tggcggcgcg cgcgaatgcg tgctggaagc gcgcggcgtc acgaagacgt tcgtgcaggg cggcttcaac gtgcaggtgc tcgacaacgc gcaggtgtcg gtgcgccgcg gcgagaagct cgcgatcgtc ggcgcgtcgg gctcgggcaa gagcacgctg ctgcacgtac tgggcgggct cgacgagccg agcgcgggac aggtgtcgct gctcggcaag ccgttcacgc agctcgccga gcgcgagcgc aacgagctgc gcaatcgcgc gctcggcttc gtctatcagt tccaccatct gctgccggaa ttcaccgcgc tcgacaacgt cgcgatgccg ctccgaatcc gccggatgtc gaccgaggag gcgcgccggc atgcgcgcga gatgctcgag caggtggggc tcggcccgcg cgcgaagcat cgcccgggcg agctgtcggg cggcgagcgc cagcgcgtcg cgatcgcgcg cgcgctcgtg accaagcccg cttgcgtgct cgccgacgag ccgaccggca acctcgacgg ctcgaccgcc gatcatgtgt tccacctgat gctcgagctg tcgcgaacgc tcgacacgag cttcgtgatc gtcacgcacg atcccgatct cgccgcgcgc tgcgaccgca tcctgcggct gcgcgacggc gtgctgcacg aggagccggc cgtgccggtg tga

Details for BupsM.10014.a.B1.GE42238

HARVESTED ON:	data unavailable
SEQUENCED ON:	3/1/2018
EXPECTED MW:	27kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMN QNHQDGGARE CVLEARGVTK TFVQGGFNVQ VLDNAQVSVR RGEKLAIVGA SGSGKSTLLH VLGGLDEPSA GQVSLLGKPF TQLAERERNE LRNRALGFVY QFHHLLPEFT ALDNVAMPLR IRRMSTEEAR RHAREMLEQV GLGPRAKHRP GELSGGERQR VAIARALVTK PACVLADEPT GNLDGSTADH VFHLMLELSR TLDTSFVIVT HDPDLAARCD RILRLRDGVL HEEPAVPV

Validated NT Sequence

atcttactca cttacaccgg cacggccggc tcctcgtgca gcacgccgtc gcgcagccgc aggatgcggt cgcagcgcgc ggcgagatcg ggatcgtgcg tgacgatcac gaagctcgtg tcgagcgttc gcgacagctc gagcatcagg tggaacacat gatcggcggt cgagccgtcg aggttgccgg tcggctcgtc ggcgagcacg caagcgggct tggtcacgag cgcgcgcgcg atcgcgacgc gctggcgctc gccgcccgac agctcgcccg ggcgatgctt cgcgcgcggg ccgagcccca cctgctcgag catctcgcgc gcatgccggc gcgcctcctc ggtcgacatc cggcggattc ggagcggcat cgcgacgttg tcgagcgcgg tgaattccgg cagcagatgg tggaactgat agacgaagcc gagcgcgcga ttgcgcagct cgttgcgctc gcgctcggcg agctgcgtga acggcttgcc gagcagcgac acctgtcccg cgctcggctc gtcgagcccg cccagtacgt gcagcagcgt gctcttgccc gagcccgacg cgccgacgat cgcgagcttc tcgccgcggc gcaccgacac ctgcgcgttg tcgagcacct gcacgttgaa gccgccctgc acgaacgtct tcgtgacgcc gcgcgcttcc agcacgcatt cgcgcgcgcc gccatcctga tggttctgat tcatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatc agaaccatca ggatggcggc gcgcgcgaat gcgtgctgga agcgcgcggc gtcacgaaga cgttcgtgca gggcggcttc aacgtgcagg tgctcgacaa cgcgcaggtg tcggtgcgcc gcggcgagaa gctcgcgatc gtcggcgcgt cgggctcggg caagagcacg ctgctgcacg tactgggcgg gctcgacgag ccgagcgcgg gacaggtgtc gctgctcggc aagccgttca cgcagctcgc cgagcgcgag cgcaacgagc tgcgcaatcg cgcgctcggc ttcgtctatc agttccacca tctgctgccg gaattcaccg cgctcgacaa cgtcgcgatg ccgctccgaa tccgccggat gtcgaccgag gaggcgcgcc ggcatgcgcg cgagatgctc gagcaggtgg ggctcggccc gcgcgcgaag catcgcccgg gcgagctgtc gggcggcgag cgccagcgcg tcgcgatcgc gcgcgcgctc gtgaccaagc ccgcttgcgt gctcgccgac gagccgaccg gcaacctcga cggctcgacc gccgatcatg tgttccacct gatgctcgag ctgtcgcgaa cgctcgacac gagcttcgtg atcgtcacgc acgatcccga tctcgccgcg cgctgcgacc gcatcctgcg gctgcgcgac ggcgtgctgc acgaggagcc ggccgtgccg gtgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat