BupsM.00863.a

Sensory box histidine kinase (BP1026B_II1445)

CENTER ID: BupsM.00863.a
ORGANISM: Burkholderia pseudomallei 1026b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsM.00863.a.B2.GE42237 PAS and kinase domains 197 709
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|884204.3.peg.5796
UniProt: A0A0H3HXW7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIDFGLWAAA ALLLLTAGVG ALGTARLSDN EADIHRASEV ISGLERLLAL ARDMETSQRG YILTGKTDYL QPYKAALPEI RKQFDALATL VQGDAVQQQR LATLRMLLEK KQHELAATIE LRKTEGFDAA QAIVSSDAGK IFMDRARVMV NEMEATVQQQ LRTLRDRARA TRDIAIGVGL ASGLLTLAVC VSFGYVMRRL LLVEAAAAHD LFEQRELLHV TLASIGDGVI ATDVGGRITF FNRVAEEMTA LCADAVVGQP IEQVLTFRHA ATQRVIDNPA RVAVERRRTA SLPPHTRFVG RSGGELPVDG NAAPTFDANG KLVGAVLVVR DVSERERAEE RFRLAVEAAP TAMIMVGRDG RIVLVNSQAE KLFGYARGDM IGHGIDMLVP ERFRAGHWAH RDAFFGNPDA RPMGEGRDLF GLRRDGSEFP VEIGLNPITT SEGAFVLCSI ADITARKRAE LELRRRSEEL ARSNQDLEQF AYVASHDLQE PLRAVAGPLQ LLQRRYQGQL DARADEFIGH AVDGATRMQS LIDDLLSYSR VGRLEDARRP VDCEHVLDEA LRNLSAAIRE SGAQVTHDPL PTVHGIEAQL VLLFQNLIGN AIKFRGKDRV ARIHLEAHTR DDAWLLRVRD NGIGIDPQYF ERIFLIFQRL HTRREYPGTG LGLALCKRIV EHHGGRIFVE SEPGQGTTFS FTLSRPGADE TRWPRDPRE
NT Sequence
ttgatcgatt tcggtttgtg ggccgccgcc gcgctgttac tgctgacggc gggcgtcggc gcgctcggca cggcccgcct gtccgacaac gaggcggata tccatcgcgc gagcgaggtg atatcgggtc tcgaacggct gctggcgctg gcccgcgaca tggagacgag ccagcgcgga tatatcctca ccggcaagac ggactacctt cagccctaca aggcggcact gcccgaaatc cgcaagcagt tcgacgcgct cgcgacgctt gtgcagggcg atgccgttca gcagcagcgg ctcgcgacgc tgcgcatgct gctcgagaaa aagcagcacg agctcgcggc gacgatcgag ctgcgcaaga cggaggggtt cgacgcggcg caggcgatcg tctcgagcga tgcgggcaag atcttcatgg accgcgcgcg cgtgatggtc aacgaaatgg aggcgaccgt ccagcagcaa ctgaggacgc tgcgcgatcg cgccagggcg acacgcgaca tcgcgatcgg cgtggggctc gcgtccgggc tgctcacgct cgccgtgtgc gtatcgttcg gctatgtgat gcgccgcctg ctgctcgtcg aggcggccgc ggcccacgat ctgttcgagc agcgcgaact gctgcacgta acgctcgcga gcatcggcga cggcgtgatc gcgacggacg tcggcggccg catcacgttc ttcaaccggg tcgccgagga gatgacggcg ctttgcgccg atgccgtcgt cggtcagccg atcgagcagg tgctgacttt tcggcatgcc gcgacgcagc gggtgatcga caatccggcg cgcgtcgcgg tcgagcgcag gcgcaccgcg agcttgcccc cccatacgcg cttcgtcggc cgcagcggcg gggaattgcc ggtggacggc aacgccgcac cgacgttcga cgcgaacggc aagctcgtcg gcgcggtgct cgtcgtgcgc gacgtgtcgg agcgcgagcg ggccgaggag cgattccggc tcgccgtcga ggccgcgccg accgcgatga tcatggtggg gcgcgacggc aggatcgtgc tcgtcaattc gcaggccgag aaattgttcg ggtacgcgcg cggcgacatg atcgggcacg gcatcgacat gctggtgccc gagcgttttc gtgcggggca ttgggcgcac cgcgacgcgt tcttcggcaa cccggacgcg cggccgatgg gcgaggggcg ggacctgttc ggtttgcgcc gcgacggcag cgagtttccg gtcgagatcg gcctgaatcc gatcacgacc tcggagggcg cgttcgtgct gtgctcgatc gccgacatca ccgcgcgcaa gcgcgcggaa ctcgaactgc gccgcagaag cgaggagctt gcgcgctcga atcaggatct cgagcagttc gcctacgtcg cgtctcacga tctgcaggag ccgttgcgcg cggtcgcggg gcccctgcaa ttgctgcagc ggcgatacca ggggcagctc gacgcgcggg ccgacgagtt catcgggcac gcggtggatg gcgcgacgag aatgcagtcg ctgatcgacg acctgctcag ctattcgcgc gtcgggcggc tcgaggacgc gcgccggccg gtcgactgcg agcacgtgct ggacgaggcg ctgcgcaacc tgagcgcggc gattcgcgaa tccggcgcgc aggtcacgca cgatccgctg ccgaccgtgc atggcatcga ggcacagctc gtgctgctgt tccagaatct gatcggcaac gcgatcaagt ttcgcggcaa ggaccgcgtc gcgcggattc atctggaagc gcacacgcgc gacgacgcat ggctgttgcg cgtgcgcgac aacggcatcg ggatcgatcc ccagtatttc gagcgcatct tcctgatttt ccagcggctg cacacgcggc gcgaatatcc gggaacgggg ctcgggctcg cgctgtgcaa gcggatcgtc gagcaccatg gcggccgcat cttcgtcgag tccgagccgg ggcagggcac gacgttttca tttacgctgt ccaggccggg tgccgatgaa acgcgttggc cgcgcgatcc gagggagtga
Details for BupsM.00863.a.B2.GE42237
HARVESTED ON: 2/23/2018
SEQUENCED ON: 3/1/2018
EXPECTED MW: 58kDa
OBSERVED MW: 58kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMR RLLLVEAAAA HDLFEQRELL HVTLASIGDG VIATDVGGRI TFFNRVAEEM TALCADAVVG QPIEQVLTFR HAATQRVIDN PARVAVERRR TASLPPHTRF VGRSGGELPV DGNAAPTFDA NGKLVGAVLV VRDVSERERA EERFRLAVEA APTAMIMVGR DGRIVLVNSQ AEKLFGYARG DMIGHGIDML VPERFRAGHW AHRDAFFGNP DARPMGEGRD LFGLRRDGSE FPVEIGLNPI TTSEGAFVLC SIADITARKR AELELRRRSE ELARSNQDLE QFXYVASHDL QEPLRAVAGP LQLLQRRYQG QLDARADEFI GHAVDGATRM QSLIDDLLSY SRVGRLEDAR RPVDCEHVLD EALRNLSAAI RESGAQVTHD PLPTVHGIEA QLVLLFQNLI GNAIKFRGKD RVARIHLEAH TRDDAWLLRV RDNGIGIDPQ YFERIFLIFQ RLHTRREYPG TGLGLALCKR IVEHHGGRIF VESEPGQGTT FSFTLSRPGA DE
Validated NT Sequence
gtttcatcgg cacccggcct ggacagcgta aatgaaaacg tcgtgccctg ccccggctcg gactcgacga agatgcggcc gccatggtgc tcgacgatcc gcttgcacag cgcgagcccg agccccgttc ccggatattc gcgccgcgtg tgcagccgct ggaaaatcag gaagatgcgc tcgaaatact ggggatcgat cccgatgccg ttgtcgcgca cgcgcaacag ccatgcgtcg tcgcgcgtgt gcgcttccag atgaatccgc gcgacgcggt ccttgccgcg aaacttgatc gcgttgccga tcagattctg gaacagcagc acgagctgtg cctcgatgcc atgcacggtc ggcagcggat cgtgcgtgac ctgcgcgccg gattcgcgaa tcgccgcgct caggttgcgc agcgcctcgt ccagcacgtg ctcgcagtcg accggccggc gcgcgtcctc gagccgcccg acgcgcgaat agctgagcag gtcgtcgatc agcgactgca ttctcgtcgc gccatccacc gcgtgcccga tgaactcgtc ggcccgcgcg tcgagctgcc cctggtatcg ccgctgcagc aattgcaggg gccccgcgac cgcgcgcaac ggctcctgca gatcgtgaga cgcgacgtag ncgaactgct cgagatcctg attcgagcgc gcaagctcct cgcttctgcg gcgcagttcg agttccgcgc gcttgcgcgc ggtgatgtcg gcgatcgagc acagcacgaa cgcgccctcc gaggtcgtga tcggattcag gccgatctcg accggaaact cgctgccgtc gcggcgcaaa ccgaacaggt cccgcccctc gcccatnggc cgcgcgtccg ggttgccgaa gaacgcgtcg cggtgcgccc aatgccccgc acgaaaacgc tcgggcacca gcatgtcgat gccgtgcccg atcatgtcgc cgcgcgcgta cccgaacaat ttctcggcct gcgaattgac gagcacgatc ctgccgtcgc gccccaccat gatcatcgcg gtcggcgcgg cctcgacggc gagccggaat cgctcctcgg cccgctcgcg ctccgacacg tcgcgcacga cgagcaccgc gccgacgagc ttgccgttcg cgtcgaacgt cggtgcggcg ttgccgtcca ccggcaattc cccgccgctg cggccgacga agcgcgtatg ggggggcaag ctcgcggtgc gcctgcgctc gaccgcgacg cgcgccggat tgtcgatcac ccgctgcgtc gcggcatgcc gaaaagtcag cacctgctcg atcggctgac cgacgacggc atcggcgcaa agcgccgtca tctcctcggc gacccggttg aagaacgtga tgcggccgcc gacgtccgtc gcgatcacgc cgtcgccgat gctcgcgagc gttacgtgca gcagttcgcg ctgctcgaac agatcgtggg ccgcggccgc ctcgacgagc agcaggcggc gcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMR RLLLVEAAAA HDLFEQRELL HVTLASIGDG VIATDVGGRI TFFNRVAEEM TALCADAVVG QPIEQVLTFR HAATQRVIDN PARVAVERRR TASLPPHTRF VGRSGGELPV DGNAAPTFDA NGKLVGAVLV VRDVSERERA EERFRLAVEA APTAMIMVGR DGRIVLVNSQ AEKLFGYARG DMIGHGIDML VPERFRAGHW AHRDAFFGNP DARPMGEGRD LFGLRRDGSE FPVEIGLNPI TTSEGAFVLC SIADITARKR AELELRRRSE ELARSNQDLE QFAYVASHDL QEPLRAVAGP LQLLQRRYQG QLDARADEFI GHAVDGATRM QSLIDDLLSY SRVGRLEDAR RPVDCEHVLD EALRNLSAAI RESGAQVTHD PLPTVHGIEA QLVLLFQNLI GNAIKFRGKD RVARIHLEAH TRDDAWLLRV RDNGIGIDPQ YFERIFLIFQ RLHTRREYPG TGLGLALCKR IVEHHGGRIF VESEPGQGTT FSFTLSRPGA DETRWPRDPR E
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgcc gcctgctgct cgtcgaggcg gccgcggccc acgatctgtt cgagcagcgc gaactgctgc acgtaacgct cgcgagcatc ggcgacggcg tgatcgcgac ggacgtcggc ggccgcatca cgttcttcaa ccgggtcgcc gaggagatga cggcgctttg cgccgatgcc gtcgtcggtc agccgatcga gcaggtgctg acttttcggc atgccgcgac gcagcgggtg atcgacaatc cggcgcgcgt cgcggtcgag cgcaggcgca ccgcgagctt gcccccccat acgcgcttcg tcggccgcag cggcggggaa ttgccggtgg acggcaacgc cgcaccgacg ttcgacgcga acggcaagct cgtcggcgcg gtgctcgtcg tgcgcgacgt gtcggagcgc gagcgggccg aggagcgatt ccggctcgcc gtcgaggccg cgccgaccgc gatgatcatg gtggggcgcg acggcaggat cgtgctcgtc aattcgcagg ccgagaaatt gttcgggtac gcgcgcggcg acatgatcgg gcacggcatc gacatgctgg tgcccgagcg ttttcgtgcg gggcattggg cgcaccgcga cgcgttcttc ggcaacccgg acgcgcggcc gatgggcgag gggcgggacc tgttcggttt gcgccgcgac ggcagcgagt ttccggtcga gatcggcctg aatccgatca cgacctcgga gggcgcgttc gtgctgtgct cgatcgccga catcaccgcg cgcaagcgcg cggaactcga actgcgccgc agaagcgagg agcttgcgcg ctcgaatcag gatctcgagc agttcgccta cgtcgcgtct cacgatctgc aggagccgtt gcgcgcggtc gcggggcccc tgcaattgct gcagcggcga taccaggggc agctcgacgc gcgggccgac gagttcatcg ggcacgcggt ggatggcgcg acgagaatgc agtcgctgat cgacgacctg ctcagctatt cgcgcgtcgg gcggctcgag gacgcgcgcc ggccggtcga ctgcgagcac gtgctggacg aggcgctgcg caacctgagc gcggcgattc gcgaatccgg cgcgcaggtc acgcacgatc cgctgccgac cgtgcatggc atcgaggcac agctcgtgct gctgttccag aatctgatcg gcaacgcgat caagtttcgc ggcaaggacc gcgtcgcgcg gattcatctg gaagcgcaca cgcgcgacga cgcatggctg ttgcgcgtgc gcgacaacgg catcgggatc gatccccagt atttcgagcg catcttcctg attttccagc ggctgcacac gcggcgcgaa tatccgggaa cggggctcgg gctcgcgctg tgcaagcgga tcgtcgagca ccatggcggc cgcatcttcg tcgagtccga gccggggcag ggcacgacgt tttcatttac gctgtccagg ccgggtgccg atgaaacgcg ttggccgcgc gatccgaggg agtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat