BupsE.19916.a

General secretory pathway protein L (gspL BPSL0016)

CENTER ID: BupsE.19916.a
ORGANISM: Burkholderia pseudomallei K96243
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsE.19916.a.B2.GE42234 GspL periplasmic domain 356 476
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272560.51.peg.1731
UniProt: Q63Z21

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTLIVLLPP REPAVPLQEW QWPELPFALV DKSGHTQRAG RAALALLPQA ATTVLIVAAR DLLMLEQALP PLKGPRLKQA LPNIIEDQLI QDPQGCHIAV DPAALDGGRR VLAVIDRAWF KFIVDAFTAA GHRHLRAVPV TRCLPPATRR DAAAAAETEA VADVALDRPA GHAAAADAPG SGHAGATANA PAPAESIVAV ALGLAATERA PSLAEEPAAL LPQAPSAPRV ELALARGALG EGFAAPVSSA VATLEALANG TPLELYELGE PGAEPQLASV RPLDDKRLLP GAAIWPFDAL VRRALDSRFD LCQFEFEFAP WRFDRATFMR LRLPLALAAT TLAIAVIGAN AHWWKLSRER DALAAQITET LLSTFPKTTT VLDPAAQMTR QLDRLRIAAG ELSPNDFLSL ASGLARSLGP LPPNGIASLD YHDRRLDVGF KPETKVDPDF AQRLARNGLT GEIDSSTGKW TIRSRS
NT Sequence
ttgagcacgt tgattgtcct attgccgccg cgcgagccgg ccgtgccgtt gcaggaatgg caatggcccg agctgccgtt cgcgctcgtc gacaagagcg gccacacgca gcgcgcgggc cgcgcggcgc tcgcgctgct gcctcaggcg gcgacgacgg tgcttatcgt cgccgcgcgc gacctgctga tgctcgagca ggcgctgccg ccgctgaaag ggccgaggct gaagcaggcg ctgccgaaca tcatcgagga tcaactgatc caggatccgc agggctgcca catcgcggtc gatccggcgg cgctcgacgg cgggcgccgc gtgctcgcgg tgatcgaccg cgcgtggttc aagttcatcg tcgacgcctt caccgcggcc gggcatcgcc atctgcgcgc ggtgcccgtc acgcgctgcc tgccgcccgc gacgcgccgc gatgcggccg cggcggccga gaccgaggct gtcgccgacg tcgcgctcga tcggccggcg gggcacgccg ccgcggccga cgcgccgggc agcggccacg cgggcgcgac cgcgaacgcc ccagcgccgg ccgagtcgat cgtcgcggtc gcgctcggcc tcgccgcgac ggagcgcgcg ccgtcgctcg ccgaggaacc ggcggcgctg ctgccgcagg cgccgagcgc gccgcgcgtc gagctcgcgc tcgcgcgcgg cgcgctcggc gaaggcttcg ccgcgccggt gtcgagcgcg gtggcgacgc tcgaagcgct cgcgaacggc acgccgctcg agctctacga gctcggcgag ccgggtgccg agccgcagct cgcttcggtg cgcccgctcg acgacaagcg cttgctgccg ggcgccgcga tctggccgtt cgacgcgctc gtgcgccgtg cgctcgattc gcgcttcgac ctctgccagt tcgaattcga gttcgcgccg tggcgcttcg accgcgcgac gttcatgcgc ctgcgcttgc cgctcgcgct cgcggcgacg acgctcgcca tcgcggtgat cggcgcgaat gcgcactggt ggaagctctc gcgcgagcgc gacgcgctcg ccgcgcagat caccgagacg ctgctctcga cgtttccgaa gacgacgacg gtgctcgatc cggccgcgca gatgacgcgc cagctcgacc ggctgcggat cgccgcgggc gagctgtcgc cgaacgattt cctgtcgctc gcgagcggcc ttgcgcgctc gctcggcccg ctgccgccga acggcatcgc atcgctcgat tatcacgatc gccggctcga cgtcggcttc aagcccgaga ccaaggtcga ccccgacttc gcgcagcgcc tcgcgcgcaa cggcctcacg ggcgagatcg acagcagcac gggcaaatgg accattcgga gccgatcatg a
Details for BupsE.19916.a.B2.GE42234
HARVESTED ON: data unavailable
SEQUENCED ON: 3/1/2018
EXPECTED MW: 14kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML SRERDALAAQ ITETLLSTFP KTTTVLDPAA QMTRQLDRLR IAAGELSPND FLSLASGLAR SLGPLPPNGI ASLDYHDRRL DVGFKPETKV DPDFAQRLAR NGLTGEIDSS TGKWTIRSRS
Validated NT Sequence
atcttactca cttatgatcg gctccgaatg gtccatttgc ccgtgctgct gtcgatctcg cccgtgaggc cgttgcgcgc gaggcgctgc gcgaagtcgg ggtcgacctt ggtctcgggc ttgaagccga cgtcgagccg gcgatcgtga taatcgagcg atgcgatgcc gttcggcggc agcgggccga gcgagcgcgc aaggccgctc gcgagcgaca ggaaatcgtt cggcgacagc tcgcccgcgg cgatccgcag ccggtcgagc tggcgcgtca tctgcgcggc cggatcgagc accgtcgtcg tcttcggaaa cgtcgagagc agcgtctcgg tgatctgcgc ggcgagcgcg tcgcgctcgc gcgagagcat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHLS RERDALAAQI TETLLSTFPK TTTVLDPAAQ MTRQLDRLRI AAGELSPNDF LSLASGLARS LGPLPPNGIA SLDYHDRRLD VGFKPETKVD PDFAQRLARN GLTGEIDSST GKWTIRSRS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctctcgc gcgagcgcga cgcgctcgcc gcgcagatca ccgagacgct gctctcgacg tttccgaaga cgacgacggt gctcgatccg gccgcgcaga tgacgcgcca gctcgaccgg ctgcggatcg ccgcgggcga gctgtcgccg aacgatttcc tgtcgctcgc gagcggcctt gcgcgctcgc tcggcccgct gccgccgaac ggcatcgcat cgctcgatta tcacgatcgc cggctcgacg tcggcttcaa gcccgagacc aaggtcgacc ccgacttcgc gcagcgcctc gcgcgcaacg gcctcacggg cgagatcgac agcagcacgg gcaaatggac cattcggagc cgatcatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t