SSGCID: Target Page

BupsE.19915.a

Uncharacterized protein (BPSS2101)

CENTER ID:	BupsE.19915.a
ORGANISM:	Burkholderia pseudomallei K96243
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsE.19915.a.B1.GE42233	full length		1	359

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272560.51.peg.5644
UniProt:	Q63IH2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQSSDPIESL LAGVAPDAPC GANLEYEQDF LRLQESATPR PEQQYGDTVI PAEAPDWSAV ERLALELTAR TKDLRVAAHL ARSWTELRGM PGYADGLRLV AGMLDRWWDD VHPRLDADGD HDPAPRANAL AEIAGAHGCA RAARRQALFD GGPSVRDAER VFDGRDGGEH GYPGGRERLI ADLVRARDGG QPTLQAAFAA LDALDAIRAR VASALGGEWV PDASDFEKAL RRIVRDGLPP PAAAPAATNG AANVANAAHG GAANGVTNSA ANGAASGGAP AFAANGRAWR DAELTSRDDV QLGLEKMCRY FELHEPSHPA PILLRRAQRL LSLDFYEIIR DLAPESLPKL DLLSGQRNE

NT Sequence

atgcagagtt cagacccgat cgaatcgttg ctcgccggcg tcgcgcccga cgcgccgtgc ggcgcgaatc tcgaatacga gcaggatttc ctgcggctgc aggagagcgc gacgccgcgg cccgaacagc agtacggcga caccgtgatc ccggccgaag cgccggactg gagcgcggtc gagcgtctcg cgctcgagct gacggcgcgc acgaaggatc tgcgcgtggc cgcgcatctc gcgcgcagtt ggaccgaact gcgcggcatg cccggctacg cggacgggct gaggctcgtc gcggggatgc tggaccggtg gtgggacgac gtgcatccgc gcctggacgc cgacggcgac cacgacccgg ccccgcgcgc gaacgcgctg gcggaaatcg cgggcgcgca cggctgcgcg cgcgccgcgc gccggcaggc gctcttcgat ggcggcccga gcgtgcgcga tgccgagcgc gtgttcgatg gccgcgacgg cggcgagcac ggctacccgg gcggccgcga gcggctgatc gccgatctgg ttcgcgcgcg cgacggcggg cagccgacgc tgcaggcggc gttcgccgcg ctcgacgcgc tcgatgcgat tcgcgcgcgc gtcgcatcgg cgctcggcgg cgaatgggtg cccgacgcga gcgacttcga gaaggcgctg cgccggatcg tgcgcgacgg gttgccgccg cccgcggccg cgcccgccgc gacaaacggc gcggcgaatg tggcgaatgc ggcgcacggc ggcgcggcaa acggcgtcac gaacagtgcc gcgaacggcg cggcttcggg cggcgcgccg gctttcgccg cgaacggccg cgcgtggcgc gatgcggagc tgacgagccg cgacgacgtt cagctcggcc tcgaaaaaat gtgccgctac ttcgagctgc acgagccgag ccatccggcg ccgatcctgc tgcgccgcgc gcagcggctg ctgtcgctcg acttctacga aatcatccgg gatctcgcgc cggagagcct gccgaagctc gacttgctga gcggccagcg caacgaatga

Details for BupsE.19915.a.B1.GE42233

HARVESTED ON:	2/23/2018
SEQUENCED ON:	3/1/2018
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMQ SSDPIESLLA GVAPDAPCGA NLEYEQDFLR LQESATPRPE QQYGDTVIPA EAPDWSAVER LALELTARTK DLRVAAHLAR SWTELRGMPG YADGLRLVAG MLDRWWDDVH PRLDADGDHD PAPRANALAE IAGAHGCARA ARRQALFDGG PSVRDAERVF DGRDGGEHGY PGGRERLIAD LVRARDGGQP TLQAAFAALD ALDAIRARVA SALGGEWVPD ASDFEKALRR IVRDGLPPPA AAPAATNGAA NVANAAHGGA ANGVTNSAAN GAASGGAPAF AANGRAWRDA ELTSRDDVQL GLEKMCRYFE LHEPSHPAPI LLRRAQRLLS LDFYEIIRDL APESLPKLD

Validated NT Sequence

agtcgagctt cggcaggctc tccggcgcga gatcccggat gatttcgtag aagtcgagcg acagcagccg ctgcgcgcgg cgcagcagga tcggcgccgg atggctcggc tcgtgcagct cgaagtagcg gcacattttt tcgaggccga gctgaacgtc gtcgcggctc gtcagctccg catcgcgcca cgcgcggccg ttcgcggcga aagccggcgc gccgcccgaa gccgcgccgt tcgcggcact gttcgtgacg ccgtttgccg cgccgccgtg cgccgcattc gccacattcg ccgcgccgtt tgtcgcggcg ggcgcggccg cgggcggcgg caacccgtcg cgcacgatcc ggcgcagcgc cttctcgaag tcgctcgcgt cgggcaccca ttcgccgccg agcgccgatg cgacgcgcgc gcgaatcgca tcgagcgcgt cgagcgcggc gaacgccgcc tgcagcgtcg gctgcccgcc gtcgcgcgcg cgaaccagat cggcgatcag ccgctcgcgg ccgcccgggt agccgtgctc gccgccgtcg cggccatcga acacgcgctc ggcatcgcgc acgctcgggc cgccatcgaa gagcgcctgc cggcgcgcgg cgcgcgcgca gccgtgcgcg cccgcgattt ccgccagcgc gttcgcgcgc ggggccgggt cgtggtcgcc gtcggcgtcc aggcgcggat gcacgtcgtc ccaccaccgg tccagcatcc ccgcgacgag cctcagcccg tccgcgtagc cgggcatgcc gcgcagttcg gtccaactgc gcgcgagatg cgcggccacg cgcagatcct tcgtgcgcgc cgtcagctcg agcgcgagac gctcgaccgc gctccagtcc ggcgcttcgg ccgggatcac ggtgtcgccg tactgctgtt cgggccgcgg cgtcgcgctc tcctgcagcc gcaggaaatc ctgctcgtat tcgagattcg cgccgcacgg cgcgtcgggc gcgacgccgg cgagcaacga ttcgatcggg tctgaactct gcatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaga gttcagaccc gatcgaatcg ttgctcgccg gcgtcgcgcc cgacgcgccg tgcggcgcga atctcgaata cgagcaggat ttcctgcggc tgcaggagag cgcgacgccg cggcccgaac agcagtacgg cgacaccgtg atcccggccg aagcgccgga ctggagcgcg gtcgagcgtc tcgcgctcga gctgacggcg cgcacgaagg atctgcgcgt ggccgcgcat ctcgcgcgca gttggaccga actgcgcggc atgcccggct acgcggacgg gctgaggctc gtcgcgggga tgctggaccg gtggtgggac gacgtgcatc cgcgcctgga cgccgacggc gaccacgacc cggccccgcg cgcgaacgcg ctggcggaaa tcgcgggcgc gcacggctgc gcgcgcgccg cgcgccggca ggcgctcttc gatggcggcc cgagcgtgcg cgatgccgag cgcgtgttcg atggccgcga cggcggcgag cacggctacc cgggcggccg cgagcggctg atcgccgatc tggttcgcgc gcgcgacggc gggcagccga cgctgcaggc ggcgttcgcc gcgctcgacg cgctcgatgc gattcgcgcg cgcgtcgcat cggcgctcgg cggcgaatgg gtgcccgacg cgagcgactt cgagaaggcg ctgcgccgga tcgtgcgcga cgggttgccg ccgcccgcgg ccgcgcccgc cgcgacaaac ggcgcggcga atgtggcgaa tgcggcgcac ggcggcgcgg caaacggcgt cacgaacagt gccgcgaacg gcgcggcttc gggcggcgcg ccggctttcg ccgcgaacgg ccgcgcgtgg cgcgatgcgg agctgacgag ccgcgacgac gttcagctcg gcctcgaaaa aatgtgccgc tacttcgagc tgcacgagcc gagccatccg gcgccgatcc tgctgcgccg cgcgcagcgg ctgctgtcgc tcgacttcta cgaaatcatc cgggatctcg cgccggagag cctgccgaag ctcgacttgc tgagcggcca gcgcaacgaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat