BupsE.19910.a

Putative exported protein (BPSL1622)

CENTER ID: BupsE.19910.a
ORGANISM: Burkholderia pseudomallei K96243
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsE.19910.a.B2.GE42228 mature protein 30 256
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272560.6.peg.1829
UniProt: Q63UI0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKSSKISKTL ICSLLAFSAT AFSIHPVLAA SPQGGKIATT IQAAQGQTDI AALFKKTNFQ MDSAAEYGKV MQAIAANESA AVTYVQDSRL SATEVQTTHP IWSGVQNGDR LGVSWSGGDF QHQRIFVYEG NGNAGKWKKA EAADIPLKQG GAPSILNQTP LMPLMPQQQV RETSASAKSV ADVVSVSLYR RVTGNIDIYT VKVGSRIQGI YGVGMTAPGW IQADGVGTDI VVFQVRASGT PGLFYSGVAS ATVIRE
NT Sequence
atgaagtcaa gcaagatatc caaaacactg atttgctcgc ttctcgcatt ttccgctacc gctttttcga ttcatcctgt tctcgcggcc tccccacagg gcggcaagat cgcgacaacg atccaggcgg ctcaaggcca aacggatatc gccgctttat tcaagaaaac gaatttccaa atggattcgg cggccgaata cggaaaggtg atgcaggcca tcgccgcaaa cgaaagcgcc gctgtcacgt atgtgcaaga cagccgcctg tcggccaccg aggttcaaac gacgcatccg atctggagcg gcgtacagaa tggcgacagg ctcggcgtca gctggagcgg cggtgatttt cagcatcaac ggatcttcgt ctacgaaggc aacggcaatg ccgggaagtg gaagaaagcc gaggcggcgg acattccgct caagcagggc ggagcgcctt ccatcctgaa tcagacgccg ctgatgccgc tgatgccgca gcagcaggtg cgcgaaacgt ccgcgtcggc caagagtgtc gcggatgtcg tttccgtgtc gctatatcga cgagtgactg gaaatatcga tatctatacg gtaaaagtgg gcagcaggat tcaaggcatt tacggtgtcg gcatgactgc tcccggctgg attcaagccg acggcgtcgg aacggatatc gtcgtatttc aggtccgcgc ctccgggaca cccggtttgt tttatagcgg cgtggcgagc gccaccgtca tccgcgagtg a
Details for BupsE.19910.a.B2.GE42228
HARVESTED ON: data unavailable
SEQUENCED ON: 3/1/2018
EXPECTED MW: 25kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA SPQGGKIATT IQAAQGQTDI AALFKKTNFQ MDSAAEYGKV MQAIAANESA AVTYVQDSRL SATEVQTTHP IWSGVQNGDR LGVSWSGGDF QHQRIFVYEG NGNAGKWKKA EAADIPLKQG GAPSILNQTP LMPLMPQQQV RETSASAKSV ADVVSVSLYR RVTGNIDIYT VKVGSRIQGI YGVGMTAPGW IQADGVGTDI VVFQVRASGT PGLFYSGVAS ATVIRE
Validated NT Sequence
atcttactca cttactcgcg gatgacggtg gcgctcgcca cgccgctata aaacaaaccg ggtgtcccgg aggcgcggac ctgaaatacg acgatatccg ttccgacgcc gtcggcttga atccagccgg gagcagtcat gccgacaccg taaatgcctt gaatcctgct gcccactttt accgtataga tatcgatatt tccagtcact cgtcgatata gcgacacgga aacgacatcc gcgacactct tggccgacgc ggacgtttcg cgcacctgct gctgcggcat cagcggcatc agcggcgtct gattcaggat ggaaggcgct ccgccctgct tgagcggaat gtccgccgcc tcggctttct tccacttccc ggcattgccg ttgccttcgt agacgaagat ccgttgatgc tgaaaatcac cgccgctcca gctgacgccg agcctgtcgc cattctgtac gccgctccag atcggatgcg tcgtttgaac ctcggtggcc gacaggcggc tgtcttgcac atacgtgaca gcggcgcttt cgtttgcggc gatggcctgc atcacctttc cgtattcggc cgccgaatcc atttggaaat tcgttttctt gaataaagcg gcgatatccg tttggccttg agccgcctgg atcgttgtcg cgatcttgcc gccctgtggg gaggccatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHAS PQGGKIATTI QAAQGQTDIA ALFKKTNFQM DSAAEYGKVM QAIAANESAA VTYVQDSRLS ATEVQTTHPI WSGVQNGDRL GVSWSGGDFQ HQRIFVYEGN GNAGKWKKAE AADIPLKQGG APSILNQTPL MPLMPQQQVR ETSASAKSVA DVVSVSLYRR VTGNIDIYTV KVGSRIQGIY GVGMTAPGWI QADGVGTDIV VFQVRASGTP GLFYSGVASA TVIRE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcctccc cacagggcgg caagatcgcg acaacgatcc aggcggctca aggccaaacg gatatcgccg ctttattcaa gaaaacgaat ttccaaatgg attcggcggc cgaatacgga aaggtgatgc aggccatcgc cgcaaacgaa agcgccgctg tcacgtatgt gcaagacagc cgcctgtcgg ccaccgaggt tcaaacgacg catccgatct ggagcggcgt acagaatggc gacaggctcg gcgtcagctg gagcggcggt gattttcagc atcaacggat cttcgtctac gaaggcaacg gcaatgccgg gaagtggaag aaagccgagg cggcggacat tccgctcaag cagggcggag cgccttccat cctgaatcag acgccgctga tgccgctgat gccgcagcag caggtgcgcg aaacgtccgc gtcggccaag agtgtcgcgg atgtcgtttc cgtgtcgcta tatcgacgag tgactggaaa tatcgatatc tatacggtaa aagtgggcag caggattcaa ggcatttacg gtgtcggcat gactgctccc ggctggattc aagccgacgg cgtcggaacg gatatcgtcg tatttcaggt ccgcgcctcc gggacacccg gtttgtttta tagcggcgtg gcgagcgcca ccgtcatccg cgagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat