BupsE.18050.a
Phosphate transport system, substrate-binding exported periplasmic protein
| CENTER ID: | BupsE.18050.a |
| ORGANISM: | Burkholderia pseudomallei K96243 |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsE.18050.a.A1.GE34317 | full length | 1 | 342 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsE.18050.a.A1.PS01695 | full length | 1 | 342 |
Structures
5WNN
DEPOSITED: 8/1/2017
DETERMINATION: XRay
CLONE: BupsE.18050.a.A1.GE34317
PROTEIN: BupsE.18050.a.A1.PS01695
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_132742 |
| PATRIC ID: | fig|272560.6.peg.1525 |
| RefSeq: | YP_107984.1 |
| UniProt: | Q63V82 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKLMQTAFAG LAGALFAVAA HADITGAGST FAMPIYTKWA ADYQQSGGAK VNYQGIGSSG GLKQIVAKTV DFAGSDAPLK DDELAKEGLF QFPTVVGGVV PVINVPGVKA GELTLSGAVL GDIYLGKIKK WNDPAIAALN PKVKLPDTDI AVVRRADGSG TSFIWTNYLS KVNDEWKSKV GEGTTVNWPT GTGGKGNDGV AAFVQRLPGA IGYVEWAYAK KNNMVYTALK NSTGTVVEPK TETFKAAAAG ANWSKSFYQI LTNQPGKEAW PVVGATFVLL HAKQDKPAQG AETLKFFDWA FKNGAKAADD LDYISLPASV ETEIRKQWKV KVTDAAGKAV AE
NT Sequence
atgaaattga tgcaaaccgc gttcgccggt ctcgccggcg cgcttttcgc ggtcgccgca cacgccgaca tcacgggcgc gggcagcacc ttcgccatgc cgatttacac gaaatgggca gccgactatc agcagtcggg cggcgcgaag gtcaactatc agggcatcgg ctcgtcgggc ggcctcaagc agatcgtcgc gaagacggtc gatttcgccg gctcggatgc gccgctcaag gatgacgagc tcgcgaagga aggcctgttc cagttcccga cggtggtcgg cggcgtcgtg cccgtcatca acgtgccggg cgtgaaggcg ggcgaactga cgctgtcggg cgcggtgctc ggcgacatct atctcggcaa gatcaagaag tggaacgatc cggccatcgc ggcgctgaac ccgaaggtca agctgccgga tacggacatc gcggtcgtgc gccgcgcgga cggctcgggc acgagcttca tctggacgaa ttacctgtcg aaggtcaacg acgagtggaa gtcgaaggtc ggcgaaggca cgacggtcaa ctggccgacc ggcacgggcg gcaagggcaa cgacggcgtc gcggcgttcg tgcagcgtct gccgggcgcg atcggctatg tcgagtgggc gtacgcgaag aagaacaaca tggtctacac cgcgctgaag aactcgacgg gcacggtggt cgagccgaag acggagacgt tcaaggcggc tgccgccggc gcgaactggt cgaagtcgtt ctaccagatc ctgacgaacc agccgggcaa ggaagcgtgg ccggtcgtcg gcgcgacgtt cgtgctgctg cacgcgaagc aggacaagcc ggcacagggc gcggaaacgc tgaagttctt cgactgggcg ttcaagaacg gcgcgaaggc ggcggacgat ctggattaca tctcgctgcc ggcgtcggtc gagacggaaa tccgcaagca gtggaaggtg aaggtgacgg acgcggccgg caaggcggtg gccgagtaa
Details for BupsE.18050.a.A1.GE34317
| HARVESTED ON: | 1/10/2012 |
| SEQUENCED ON: | 1/17/2012 |
| EXPECTED MW: | 38kDa |
| OBSERVED MW: | 38kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 72 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKLMQTAFA GLAGALFAVA AHADITGAGS TFAMPIYTKW AADYQQSGGA KVNYQGIGSS GGLKQIVAKT VDFAGSDAPL KDDELAKEGL FQFPTVVGGV VPVINVPGVK AGELTLSGAV LGDIYLGKIK KWNDPAIAAL NPKVKLPDTD IAVVRRADGS GTSFIWTNYL SKVNDEWKSK VGEGTTVNWP TGTGGKGNDG VAAFVQRLPG AIGYVEWAYA KKNNMVYTAL KNSTGTVVEP XXET
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaat tgatgcaaac cgcgttcgcc ggtctcgccg gcgcgctttt cgcggtcgcc gcacacgccg acatcacggg cgcgggcagc accttcgcca tgccgattta cacgaaatgg gcagccgact atcagcagtc gggcggcgcg aaggtcaact atcagggcat cggctcgtcg ggcggcctca agcagatcgt cgcgaagacg gtcgatttcg ccggctcgga tgcgccgctc aaggatgacg agctcgcgaa ggaaggcctg ttccagttcc cgacggtggt cggcggcgtc gtgcccgtca tcaacgtgcc gggcgtgaag gcgggcgaac tgacgctgtc gggcgcggtg ctcggcgaca tctatctcgg caagatcaag aagtggaacg atccggccat cgcggcgctg aacccgaagg tcaagctgcc ggatacggac atcgcggtcg tgcgccgcgc ggacggctcg ggcacgagct tcatctggac gaattacctg tcgaaggtca acgacgagtg gaagtcgaag gtcggcgaag gcacgacggt caactggccg accggcacgg gcggcaaggg caacgacggc gtcgcggcgt tcgtgcagcg tctgccgggc gcgatcggct atgtcgagtg ggcgtacgcg aagaagaaca acatggtcta caccgcgctg aagaactcga cgggcacngt ggtcgagccg aananggaga cgtt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKLMQTAFA GLAGALFAVA AHADITGAGS TFAMPIYTKW AADYQQSGGA KVNYQGIGSS GGLKQIVAKT VDFAGSDAPL KDDELAKEGL FQFPTVVGGV VPVINVPGVK AGELTLSGAV LGDIYLGKIK KWNDPAIAAL NPKVKLPDTD IAVVRRADGS GTSFIWTNYL SKVNDEWKSK VGEGTTVNWP TGTGGKGNDG VAAFVQRLPG AIGYVEWAYA KKNNMVYTAL KNSTGTVVEP KTETFKAAAA GANWSKSFYQ ILTNQPGKEA WPVVGATFVL LHAKQDKPAQ GAETLKFFDW AFKNGAKAAD DLDYISLPAS VETEIRKQWK VKVTDAAGKA VAE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaattgatg caaaccgcgt tcgccggtct cgccggcgcg cttttcgcgg tcgccgcaca cgccgacatc acgggcgcgg gcagcacctt cgccatgccg atttacacga aatgggcagc cgactatcag cagtcgggcg gcgcgaaggt caactatcag ggcatcggct cgtcgggcgg cctcaagcag atcgtcgcga agacggtcga tttcgccggc tcggatgcgc cgctcaagga tgacgagctc gcgaaggaag gcctgttcca gttcccgacg gtggtcggcg gcgtcgtgcc cgtcatcaac gtgccgggcg tgaaggcggg cgaactgacg ctgtcgggcg cggtgctcgg cgacatctat ctcggcaaga tcaagaagtg gaacgatccg gccatcgcgg cgctgaaccc gaaggtcaag ctgccggata cggacatcgc ggtcgtgcgc cgcgcggacg gctcgggcac gagcttcatc tggacgaatt acctgtcgaa ggtcaacgac gagtggaagt cgaaggtcgg cgaaggcacg acggtcaact ggccgaccgg cacgggcggc aagggcaacg acggcgtcgc ggcgttcgtg cagcgtctgc cgggcgcgat cggctatgtc gagtgggcgt acgcgaagaa gaacaacatg gtctacaccg cgctgaagaa ctcgacgggc acggtggtcg agccgaagac ggagacgttc aaggcggctg ccgccggcgc gaactggtcg aagtcgttct accagatcct gacgaaccag ccgggcaagg aagcgtggcc ggtcgtcggc gcgacgttcg tgctgctgca cgcgaagcag gacaagccgg cacagggcgc ggaaacgctg aagttcttcg actgggcgtt caagaacggc gcgaaggcgg cggacgatct ggattacatc tcgctgccgg cgtcggtcga gacggaaatc cgcaagcagt ggaaggtgaa ggtgacggac gcggccggca aggcggtggc cgagtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BupsE.18050.a.A1.PS01695
| PURIFICATION DATe: | 12/4/2012 |
| CONCENTRATION: | 54.7mg/ml |
| OBSERVED MW: | 36kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 72 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKLMQTAFA GLAGALFAVA AHADITGAGS TFAMPIYTKW AADYQQSGGA KVNYQGIGSS GGLKQIVAKT VDFAGSDAPL KDDELAKEGL FQFPTVVGGV VPVINVPGVK AGELTLSGAV LGDIYLGKIK KWNDPAIAAL NPKVKLPDTD IAVVRRADGS GTSFIWTNYL SKVNDEWKSK VGEGTTVNWP TGTGGKGNDG VAAFVQRLPG AIGYVEWAYA KKNNMVYTAL KNSTGTVVEP XXET
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaat tgatgcaaac cgcgttcgcc ggtctcgccg gcgcgctttt cgcggtcgcc gcacacgccg acatcacggg cgcgggcagc accttcgcca tgccgattta cacgaaatgg gcagccgact atcagcagtc gggcggcgcg aaggtcaact atcagggcat cggctcgtcg ggcggcctca agcagatcgt cgcgaagacg gtcgatttcg ccggctcgga tgcgccgctc aaggatgacg agctcgcgaa ggaaggcctg ttccagttcc cgacggtggt cggcggcgtc gtgcccgtca tcaacgtgcc gggcgtgaag gcgggcgaac tgacgctgtc gggcgcggtg ctcggcgaca tctatctcgg caagatcaag aagtggaacg atccggccat cgcggcgctg aacccgaagg tcaagctgcc ggatacggac atcgcggtcg tgcgccgcgc ggacggctcg ggcacgagct tcatctggac gaattacctg tcgaaggtca acgacgagtg gaagtcgaag gtcggcgaag gcacgacggt caactggccg accggcacgg gcggcaaggg caacgacggc gtcgcggcgt tcgtgcagcg tctgccgggc gcgatcggct atgtcgagtg ggcgtacgcg aagaagaaca acatggtcta caccgcgctg aagaactcga cgggcacngt ggtcgagccg aananggaga cgtt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKLMQTAFA GLAGALFAVA AHADITGAGS TFAMPIYTKW AADYQQSGGA KVNYQGIGSS GGLKQIVAKT VDFAGSDAPL KDDELAKEGL FQFPTVVGGV VPVINVPGVK AGELTLSGAV LGDIYLGKIK KWNDPAIAAL NPKVKLPDTD IAVVRRADGS GTSFIWTNYL SKVNDEWKSK VGEGTTVNWP TGTGGKGNDG VAAFVQRLPG AIGYVEWAYA KKNNMVYTAL KNSTGTVVEP KTETFKAAAA GANWSKSFYQ ILTNQPGKEA WPVVGATFVL LHAKQDKPAQ GAETLKFFDW AFKNGAKAAD DLDYISLPAS VETEIRKQWK VKVTDAAGKA VAE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAATTGATG CAAACCGCGT TCGCCGGTCT CGCCGGCGCG CTTTTCGCGG TCGCCGCACA CGCCGACATC ACGGGCGCGG GCAGCACCTT CGCCATGCCG ATTTACACGA AATGGGCAGC CGACTATCAG CAGTCGGGCG GCGCGAAGGT CAACTATCAG GGCATCGGCT CGTCGGGCGG CCTCAAGCAG ATCGTCGCGA AGACGGTCGA TTTCGCCGGC TCGGATGCGC CGCTCAAGGA TGACGAGCTC GCGAAGGAAG GCCTGTTCCA GTTCCCGACG GTGGTCGGCG GCGTCGTGCC CGTCATCAAC GTGCCGGGCG TGAAGGCGGG CGAACTGACG CTGTCGGGCG CGGTGCTCGG CGACATCTAT CTCGGCAAGA TCAAGAAGTG GAACGATCCG GCCATCGCGG CGCTGAACCC GAAGGTCAAG CTGCCGGATA CGGACATCGC GGTCGTGCGC CGCGCGGACG GCTCGGGCAC GAGCTTCATC TGGACGAATT ACCTGTCGAA GGTCAACGAC GAGTGGAAGT CGAAGGTCGG CGAAGGCACG ACGGTCAACT GGCCGACCGG CACGGGCGGC AAGGGCAACG ACGGCGTCGC GGCGTTCGTG CAGCGTCTGC CGGGCGCGAT CGGCTATGTC GAGTGGGCGT ACGCGAAGAA GAACAACATG GTCTACACCG CGCTGAAGAA CTCGACGGGC ACGGTGGTCG AGCCGAAGAC GGAGACGTTC AAGGCGGCTG CCGCCGGCGC GAACTGGTCG AAGTCGTTCT ACCAGATCCT GACGAACCAG CCGGGCAAGG AAGCGTGGCC GGTCGTCGGC GCGACGTTCG TGCTGCTGCA CGCGAAGCAG GACAAGCCGG CACAGGGCGC GGAAACGCTG AAGTTCTTCG ACTGGGCGTT CAAGAACGGC GCGAAGGCGG CGGACGATCT GGATTACATC TCGCTGCCGG CGTCGGTCGA GACGGAAATC CGCAAGCAGT GGAAGGTGAA GGTGACGGAC GCGGCCGGCA AGGCGGTGGC CGAGTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA