BupsE.18028.a
Putative exported protein
| CENTER ID: | BupsE.18028.a |
| ORGANISM: | Burkholderia pseudomallei K96243 |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsE.18028.a.A1.GE34311 | full length | 1 | 208 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsE.18028.a.A1.PS01510 | full length | 1 | 208 |
Structures
4JGB
DEPOSITED: 2/28/2013
DETERMINATION: XRay
CLONE: BupsE.18028.a.A1.GE34311
PROTEIN: BupsE.18028.a.A1.PS01510
Citations by Others
| TAK1: a potent tumour necrosis factor inhibitor for the treatment of inflammatory diseases | |
| J Totzke, SA Scarneo, KW Yang- Open, 2020 - royalsocietypublishing.org | |
| cited by: | 53 others |
| PDB: 4JGB | |
| Targeting Transforming Growth Factor Beta-Activated Kinase 1 as a Therapeutic | |
| J Totzke - 2017 - dukespace.lib.duke.edu | |
| PDB: 4JGB |
This list was obtained from Google Scholar searches using the Open Source tool https://github.com/ckreibich/scholar.py and each citation has been manually reviewed.
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|272560.6.peg.1413 |
| RefSeq: | YP_107894.1 |
| UniProt: | Q63VH2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKIALFGATG MIGSRIAAEA ARRGHQVTAL SRNPAASGAN VQAKAADLFD PASIAAALAG QDVVASAYGP KQEEASKVVA VAKALVDGAR KAGVKRVVVV GGAGTLEVAP GKQLVDTEGF PDAYKAVALA HRDAYGYLST VQDLDWTFFS PAALIAPGER TGRFRTGAGR LIVDEQGNSK ISAEDYAIAF VDEIEQGRFI RQAATAAY
NT Sequence
ttgaaaatcg ccctgttcgg cgcaaccggc atgatcggct cgcggatcgc cgccgaagcg gcgcgccgcg gccatcaggt gacggcgctg tcgcgcaacc cggcggcgag cggcgcgaac gtgcaggcga aggcggccga cctgttcgat ccggcgagca tcgccgccgc gctcgcaggc caggacgtcg tcgcgagcgc ctatgggccg aagcaggagg aggcatcgaa ggtcgtcgcg gtcgcgaagg cgctcgtcga cggcgcgcgc aaggcgggcg tgaagcgcgt cgtcgtcgtg ggcggcgcgg gcacgctcga agtcgctccc ggcaagcagc tcgtcgatac cgagggcttt ccggatgcgt acaaggccgt cgcgctcgcg caccgcgacg cgtacggcta cctgtcgacc gtgcaggacc tcgactggac gttcttctcg cccgccgcgc tgatcgcgcc cggcgagcgt accggccggt tccgcaccgg cgcgggcagg ctgatcgtcg acgagcaagg caacagcaag atctccgccg aagactatgc gatcgcgttc gtcgacgaga tcgagcaagg ccgtttcatc cggcaagccg cgacggcggc gtactga
Details for BupsE.18028.a.A1.GE34311
| HARVESTED ON: | 1/10/2012 |
| SEQUENCED ON: | 1/13/2012 |
| EXPECTED MW: | 23kDa |
| OBSERVED MW: | 25kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLKIALFGA TGMIGSRIAA EAARRGHQVT ALSRNPAASG ANVQAKAADL FDPASIAAAL AGQDVVASAY GPKQEEASKV VAVAKALVDG ARKAGVKRVV VVGGAGTLEV APGKQLVDTE GFPDAYKAVA LAHRDAYGYL STVQDLDWTF FSPAALIAPG ERTGRFRTGA GRLIVDEQGN SKISAEDYAI AFVDEIEQGR FIRQAATAAY
Validated NT Sequence
tttagtacgc cgccgtcgcg gcttgccgga tgaaacggcc ttgctcgatc tcgtcgacga acgcgatcgc atagtcttcg gcggagatct tgctgttgcc ttgctcgtcg acgatcagcc tgcccgcgcc ggtgcggaac cggccggtac gctcgccggg cgcgatcagc gcggcgggcg agaagaacgt ccagtcgagg tcctgcacgg tcgacaggta gccgtacgcg tcgcggtgcg cgagcgcgac ggccttgtac gcatccggaa agccctcggt atcgacgagc tgcttgccgg gagcgacttc gagcgtgccc gcgccgccca cgacgacgac gcgcttcacg cccgccttgc gcgcgccgtc gacgagcgcc ttcgcgaccg cgacgacctt cgatgcctcc tcctgcttcg gcccataggc gctcgcgacg acgtcctggc ctgcgagcgc ggcggcgatg ctcgccggat cgaacaggtc ggccgccttc gcctgcacgt tcgcgccgct cgccgccggg ttgcgcgaca gcgccgtcac ctgatggccg cggcgcgccg cttcggcggc gatccgcgag ccgatcatgc cggttgcgcc gaacagggcg attttcaaca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLKIALFGA TGMIGSRIAA EAARRGHQVT ALSRNPAASG ANVQAKAADL FDPASIAAAL AGQDVVASAY GPKQEEASKV VAVAKALVDG ARKAGVKRVV VVGGAGTLEV APGKQLVDTE GFPDAYKAVA LAHRDAYGYL STVQDLDWTF FSPAALIAPG ERTGRFRTGA GRLIVDEQGN SKISAEDYAI AFVDEIEQGR FIRQAATAAY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgaaaatc gccctgttcg gcgcaaccgg catgatcggc tcgcggatcg ccgccgaagc ggcgcgccgc ggccatcagg tgacggcgct gtcgcgcaac ccggcggcga gcggcgcgaa cgtgcaggcg aaggcggccg acctgttcga tccggcgagc atcgccgccg cgctcgcagg ccaggacgtc gtcgcgagcg cctatgggcc gaagcaggag gaggcatcga aggtcgtcgc ggtcgcgaag gcgctcgtcg acggcgcgcg caaggcgggc gtgaagcgcg tcgtcgtcgt gggcggcgcg ggcacgctcg aagtcgctcc cggcaagcag ctcgtcgata ccgagggctt tccggatgcg tacaaggccg tcgcgctcgc gcaccgcgac gcgtacggct acctgtcgac cgtgcaggac ctcgactgga cgttcttctc gcccgccgcg ctgatcgcgc ccggcgagcg taccggccgg ttccgcaccg gcgcgggcag gctgatcgtc gacgagcaag gcaacagcaa gatctccgcc gaagactatg cgatcgcgtt cgtcgacgag atcgagcaag gccgtttcat ccggcaagcc gcgacggcgg cgtactaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BupsE.18028.a.A1.PS01510
| PURIFICATION DATe: | 5/22/2012 |
| CONCENTRATION: | 46.2mg/ml |
| OBSERVED MW: | 25kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLKIALFGA TGMIGSRIAA EAARRGHQVT ALSRNPAASG ANVQAKAADL FDPASIAAAL AGQDVVASAY GPKQEEASKV VAVAKALVDG ARKAGVKRVV VVGGAGTLEV APGKQLVDTE GFPDAYKAVA LAHRDAYGYL STVQDLDWTF FSPAALIAPG ERTGRFRTGA GRLIVDEQGN SKISAEDYAI AFVDEIEQGR FIRQAATAAY
Validated NT Sequence
tttagtacgc cgccgtcgcg gcttgccgga tgaaacggcc ttgctcgatc tcgtcgacga acgcgatcgc atagtcttcg gcggagatct tgctgttgcc ttgctcgtcg acgatcagcc tgcccgcgcc ggtgcggaac cggccggtac gctcgccggg cgcgatcagc gcggcgggcg agaagaacgt ccagtcgagg tcctgcacgg tcgacaggta gccgtacgcg tcgcggtgcg cgagcgcgac ggccttgtac gcatccggaa agccctcggt atcgacgagc tgcttgccgg gagcgacttc gagcgtgccc gcgccgccca cgacgacgac gcgcttcacg cccgccttgc gcgcgccgtc gacgagcgcc ttcgcgaccg cgacgacctt cgatgcctcc tcctgcttcg gcccataggc gctcgcgacg acgtcctggc ctgcgagcgc ggcggcgatg ctcgccggat cgaacaggtc ggccgccttc gcctgcacgt tcgcgccgct cgccgccggg ttgcgcgaca gcgccgtcac ctgatggccg cggcgcgccg cttcggcggc gatccgcgag ccgatcatgc cggttgcgcc gaacagggcg attttcaaca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLKIALFGA TGMIGSRIAA EAARRGHQVT ALSRNPAASG ANVQAKAADL FDPASIAAAL AGQDVVASAY GPKQEEASKV VAVAKALVDG ARKAGVKRVV VVGGAGTLEV APGKQLVDTE GFPDAYKAVA LAHRDAYGYL STVQDLDWTF FSPAALIAPG ERTGRFRTGA GRLIVDEQGN SKISAEDYAI AFVDEIEQGR FIRQAATAAY
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGAAAATC GCCCTGTTCG GCGCAACCGG CATGATCGGC TCGCGGATCG CCGCCGAAGC GGCGCGCCGC GGCCATCAGG TGACGGCGCT GTCGCGCAAC CCGGCGGCGA GCGGCGCGAA CGTGCAGGCG AAGGCGGCCG ACCTGTTCGA TCCGGCGAGC ATCGCCGCCG CGCTCGCAGG CCAGGACGTC GTCGCGAGCG CCTATGGGCC GAAGCAGGAG GAGGCATCGA AGGTCGTCGC GGTCGCGAAG GCGCTCGTCG ACGGCGCGCG CAAGGCGGGC GTGAAGCGCG TCGTCGTCGT GGGCGGCGCG GGCACGCTCG AAGTCGCTCC CGGCAAGCAG CTCGTCGATA CCGAGGGCTT TCCGGATGCG TACAAGGCCG TCGCGCTCGC GCACCGCGAC GCGTACGGCT ACCTGTCGAC CGTGCAGGAC CTCGACTGGA CGTTCTTCTC GCCCGCCGCG CTGATCGCGC CCGGCGAGCG TACCGGCCGG TTCCGCACCG GCGCGGGCAG GCTGATCGTC GACGAGCAAG GCAACAGCAA GATCTCCGCC GAAGACTATG CGATCGCGTT CGTCGACGAG ATCGAGCAAG GCCGTTTCAT CCGGCAAGCC GCGACGGCGG CGTACTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA