BupsE.18018.a

Putative uncharacterized protein

CENTER ID: BupsE.18018.a
ORGANISM: Burkholderia pseudomallei K96243
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsE.18018.a.A1.GE34309 full length 1 215
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsE.18018.a.A1.PW37285 full length 1 215
BupsE.18018.a.A1.PW37363 full length 1 215

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272560.6.peg.6320
RefSeq: YP_111964.1
UniProt: Q63IV8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKSDAALKQD VEQELYWDPS LDARQIDVSV RDRIVTLRGF VPSWAQKYAA QKAAQRVAGA RALVLELKVL PSEAPRDDEV LAAAIVSALA WQEALAGQEI HVDVDEGCVT LTGEVAFGYQ RQAAETVVSR MRGVIGVVNR IDVRSRDAGA TVRERVADAL ARRAQREAAH IEVDERDGIV TLTGTVDSLI ERRAACGAAW SVKGVHEVID HLSVA
NT Sequence
atgaaatccg atgctgcgct caagcaggat gtcgaacagg aactgtattg ggatccgtcg ctggatgcgc gacagatcga cgtgtcggtg cgcgatcgca tcgtgacgct gcgcggcttc gtgccgagct gggcgcagaa gtacgcggcg cagaaggccg cgcagcgcgt cgcgggcgcg cgggcgctgg tgctcgagct gaaggtgctg ccgagcgaag cgccgcgcga cgacgaagtg ctcgccgccg cgatcgtgtc ggcgctcgcg tggcaggaag cgctcgccgg gcaggagatc cacgtcgatg tggacgaagg ctgcgtcacg ctcacgggcg aagtggcgtt cggctaccag cggcaggcgg ccgagacggt cgtgagccgg atgcgcggcg tgatcggcgt cgtcaaccgg atcgacgtgc gttcgcggga cgccggcgcg accgtgcgcg aacgtgtggc ggatgcgctt gcgcgacgcg cgcagcgcga ggccgcgcac atcgaagtcg acgagcgcga cggcatcgtc acgctgacgg gcaccgtcga ttcgctgatc gagcggcgcg cggcttgcgg tgccgcatgg tcggtgaagg gcgtgcacga agtgatcgat catctgagcg tcgcatag
Details for BupsE.18018.a.A1.GE34309
HARVESTED ON: 1/10/2012
SEQUENCED ON: 1/13/2012
EXPECTED MW: 25kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKSDAALKQ DVEQELYWDP SLDARQIDVS VRDRIVTLRG FVPSWAQKYA AQKAAQRVAG ARALVLELKV LPSEAPRDDE VLAAAIVSAL AWQEALAGQE IHVDVDEGCV TLTGEVAFGY QRQAAETVVS RMRGVIGVVN RIDVRSRDAG ATVRERVADA LARRAQREAA HIEVDERDGI VTLTGTVDSL IERRAACGAA WSVKGVHEVI DHLSVA
Validated NT Sequence
ttattatgcg acgctcagat gatcgatcac ttcgtgcacg cccttcaccg accatgcggc accgcaagcc gcgcgccgct cgatcagcga atcgacggtg cccgtcagcg tgacgatgcc gtcgcgctcg tcgacttcga tgtgcgcggc ctcgcgctgc gcgcgtcgcg caagcgcatc cgccacacgt tcgcgcacgg tcgcgccggc gtcccgcgaa cgcacgtcga tccggttgac gacgccgatc acgccgcgca tccggctcac gaccgtctcg gccgcctgcc gctggtagcc gaacgccact tcgcccgtga gcgtgacgca gccttcgtcc acatcgacgt ggatctcctg cccggcgagc gcttcctgcc acgcgagcgc cgacacgatc gcggcggcga gcacttcgtc gtcgcgcggc gcttcgctcg gcagcacctt cagctcgagc accagcgccc gcgcgcccgc gacgcgctgc gcggccttct gcgccgcgta cttctgcgcc cagctcggca cgaagccgcg cagcgtcacg atgcgatcgc gcaccgacac gtcgatctgt cgcgcatcca gcgacggatc ccaatacagt tcctgttcga catcctgctt gagcgcagca tcggatttca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKSDAALKQ DVEQELYWDP SLDARQIDVS VRDRIVTLRG FVPSWAQKYA AQKAAQRVAG ARALVLELKV LPSEAPRDDE VLAAAIVSAL AWQEALAGQE IHVDVDEGCV TLTGEVAFGY QRQAAETVVS RMRGVIGVVN RIDVRSRDAG ATVRERVADA LARRAQREAA HIEVDERDGI VTLTGTVDSL IERRAACGAA WSVKGVHEVI DHLSVA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaatccgat gctgcgctca agcaggatgt cgaacaggaa ctgtattggg atccgtcgct ggatgcgcga cagatcgacg tgtcggtgcg cgatcgcatc gtgacgctgc gcggcttcgt gccgagctgg gcgcagaagt acgcggcgca gaaggccgcg cagcgcgtcg cgggcgcgcg ggcgctggtg ctcgagctga aggtgctgcc gagcgaagcg ccgcgcgacg acgaagtgct cgccgccgcg atcgtgtcgg cgctcgcgtg gcaggaagcg ctcgccgggc aggagatcca cgtcgatgtg gacgaaggct gcgtcacgct cacgggcgaa gtggcgttcg gctaccagcg gcaggcggcc gagacggtcg tgagccggat gcgcggcgtg atcggcgtcg tcaaccggat cgacgtgcgt tcgcgggacg ccggcgcgac cgtgcgcgaa cgtgtggcgg atgcgcttgc gcgacgcgcg cagcgcgagg ccgcgcacat cgaagtcgac gagcgcgacg gcatcgtcac gctgacgggc accgtcgatt cgctgatcga gcggcgcgcg gcttgcggtg ccgcatggtc ggtgaagggc gtgcacgaag tgatcgatca tctgagcgtc gcataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BupsE.18018.a.A1.PW37285
PURIFICATION DATe: 5/6/2013
CONCENTRATION: 2.75mg/ml
OBSERVED MW: 28kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKSDAALKQ DVEQELYWDP SLDARQIDVS VRDRIVTLRG FVPSWAQKYA AQKAAQRVAG ARALVLELKV LPSEAPRDDE VLAAAIVSAL AWQEALAGQE IHVDVDEGCV TLTGEVAFGY QRQAAETVVS RMRGVIGVVN RIDVRSRDAG ATVRERVADA LARRAQREAA HIEVDERDGI VTLTGTVDSL IERRAACGAA WSVKGVHEVI DHLSVA
Validated NT Sequence
ttattatgcg acgctcagat gatcgatcac ttcgtgcacg cccttcaccg accatgcggc accgcaagcc gcgcgccgct cgatcagcga atcgacggtg cccgtcagcg tgacgatgcc gtcgcgctcg tcgacttcga tgtgcgcggc ctcgcgctgc gcgcgtcgcg caagcgcatc cgccacacgt tcgcgcacgg tcgcgccggc gtcccgcgaa cgcacgtcga tccggttgac gacgccgatc acgccgcgca tccggctcac gaccgtctcg gccgcctgcc gctggtagcc gaacgccact tcgcccgtga gcgtgacgca gccttcgtcc acatcgacgt ggatctcctg cccggcgagc gcttcctgcc acgcgagcgc cgacacgatc gcggcggcga gcacttcgtc gtcgcgcggc gcttcgctcg gcagcacctt cagctcgagc accagcgccc gcgcgcccgc gacgcgctgc gcggccttct gcgccgcgta cttctgcgcc cagctcggca cgaagccgcg cagcgtcacg atgcgatcgc gcaccgacac gtcgatctgt cgcgcatcca gcgacggatc ccaatacagt tcctgttcga catcctgctt gagcgcagca tcggatttca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKSDAALKQ DVEQELYWDP SLDARQIDVS VRDRIVTLRG FVPSWAQKYA AQKAAQRVAG ARALVLELKV LPSEAPRDDE VLAAAIVSAL AWQEALAGQE IHVDVDEGCV TLTGEVAFGY QRQAAETVVS RMRGVIGVVN RIDVRSRDAG ATVRERVADA LARRAQREAA HIEVDERDGI VTLTGTVDSL IERRAACGAA WSVKGVHEVI DHLSVA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAATCCGAT GCTGCGCTCA AGCAGGATGT CGAACAGGAA CTGTATTGGG ATCCGTCGCT GGATGCGCGA CAGATCGACG TGTCGGTGCG CGATCGCATC GTGACGCTGC GCGGCTTCGT GCCGAGCTGG GCGCAGAAGT ACGCGGCGCA GAAGGCCGCG CAGCGCGTCG CGGGCGCGCG GGCGCTGGTG CTCGAGCTGA AGGTGCTGCC GAGCGAAGCG CCGCGCGACG ACGAAGTGCT CGCCGCCGCG ATCGTGTCGG CGCTCGCGTG GCAGGAAGCG CTCGCCGGGC AGGAGATCCA CGTCGATGTG GACGAAGGCT GCGTCACGCT CACGGGCGAA GTGGCGTTCG GCTACCAGCG GCAGGCGGCC GAGACGGTCG TGAGCCGGAT GCGCGGCGTG ATCGGCGTCG TCAACCGGAT CGACGTGCGT TCGCGGGACG CCGGCGCGAC CGTGCGCGAA CGTGTGGCGG ATGCGCTTGC GCGACGCGCG CAGCGCGAGG CCGCGCACAT CGAAGTCGAC GAGCGCGACG GCATCGTCAC GCTGACGGGC ACCGTCGATT CGCTGATCGA GCGGCGCGCG GCTTGCGGTG CCGCATGGTC GGTGAAGGGC GTGCACGAAG TGATCGATCA TCTGAGCGTC GCATAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA
Details for BupsE.18018.a.A1.PW37363
PURIFICATION DATe: 9/16/2013
CONCENTRATION: 2.5mg/ml
OBSERVED MW: 27kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKSDAALKQ DVEQELYWDP SLDARQIDVS VRDRIVTLRG FVPSWAQKYA AQKAAQRVAG ARALVLELKV LPSEAPRDDE VLAAAIVSAL AWQEALAGQE IHVDVDEGCV TLTGEVAFGY QRQAAETVVS RMRGVIGVVN RIDVRSRDAG ATVRERVADA LARRAQREAA HIEVDERDGI VTLTGTVDSL IERRAACGAA WSVKGVHEVI DHLSVA
Validated NT Sequence
ttattatgcg acgctcagat gatcgatcac ttcgtgcacg cccttcaccg accatgcggc accgcaagcc gcgcgccgct cgatcagcga atcgacggtg cccgtcagcg tgacgatgcc gtcgcgctcg tcgacttcga tgtgcgcggc ctcgcgctgc gcgcgtcgcg caagcgcatc cgccacacgt tcgcgcacgg tcgcgccggc gtcccgcgaa cgcacgtcga tccggttgac gacgccgatc acgccgcgca tccggctcac gaccgtctcg gccgcctgcc gctggtagcc gaacgccact tcgcccgtga gcgtgacgca gccttcgtcc acatcgacgt ggatctcctg cccggcgagc gcttcctgcc acgcgagcgc cgacacgatc gcggcggcga gcacttcgtc gtcgcgcggc gcttcgctcg gcagcacctt cagctcgagc accagcgccc gcgcgcccgc gacgcgctgc gcggccttct gcgccgcgta cttctgcgcc cagctcggca cgaagccgcg cagcgtcacg atgcgatcgc gcaccgacac gtcgatctgt cgcgcatcca gcgacggatc ccaatacagt tcctgttcga catcctgctt gagcgcagca tcggatttca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKSDAALKQ DVEQELYWDP SLDARQIDVS VRDRIVTLRG FVPSWAQKYA AQKAAQRVAG ARALVLELKV LPSEAPRDDE VLAAAIVSAL AWQEALAGQE IHVDVDEGCV TLTGEVAFGY QRQAAETVVS RMRGVIGVVN RIDVRSRDAG ATVRERVADA LARRAQREAA HIEVDERDGI VTLTGTVDSL IERRAACGAA WSVKGVHEVI DHLSVA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAATCCGAT GCTGCGCTCA AGCAGGATGT CGAACAGGAA CTGTATTGGG ATCCGTCGCT GGATGCGCGA CAGATCGACG TGTCGGTGCG CGATCGCATC GTGACGCTGC GCGGCTTCGT GCCGAGCTGG GCGCAGAAGT ACGCGGCGCA GAAGGCCGCG CAGCGCGTCG CGGGCGCGCG GGCGCTGGTG CTCGAGCTGA AGGTGCTGCC GAGCGAAGCG CCGCGCGACG ACGAAGTGCT CGCCGCCGCG ATCGTGTCGG CGCTCGCGTG GCAGGAAGCG CTCGCCGGGC AGGAGATCCA CGTCGATGTG GACGAAGGCT GCGTCACGCT CACGGGCGAA GTGGCGTTCG GCTACCAGCG GCAGGCGGCC GAGACGGTCG TGAGCCGGAT GCGCGGCGTG ATCGGCGTCG TCAACCGGAT CGACGTGCGT TCGCGGGACG CCGGCGCGAC CGTGCGCGAA CGTGTGGCGG ATGCGCTTGC GCGACGCGCG CAGCGCGAGG CCGCGCACAT CGAAGTCGAC GAGCGCGACG GCATCGTCAC GCTGACGGGC ACCGTCGATT CGCTGATCGA GCGGCGCGCG GCTTGCGGTG CCGCATGGTC GGTGAAGGGC GTGCACGAAG TGATCGATCA TCTGAGCGTC GCATAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA