SSGCID: Target Page

BupsE.18016.a

Bifunctional PutA protein [includes: proline dehydrogenase (EC 1.5.99.8) (Proline oxidase); delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12) (P5c dehydrogenase)] (EC 1.5.99.8)

CENTER ID:	BupsE.18016.a
ORGANISM:	Burkholderia pseudomallei K96243
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsE.18016.a.A2.GE34337	domain 1		1	607

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsE.18016.a.A2.PW34947	domain 1		1	607

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272560.6.peg.3854
RefSeq:	YP_109981.1
UniProt:	Q63PI8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MASTTLGVKV DDLLRARLKD AASRLERTPH WLIKQAIFAY LERIEHGQLP PELSGHTGVA DLSDSHADGD DDGAPHPFLE FAQSVQPQSV LRAAITAAYR RPEPECVPFL IGEARLPASL ASDVQAMAAG LVEALREKSS GGGVEGLIHE FSLSSQEGVA LMCLAEALLR IPDRATRDAL IRDKISKGDW RSHVGHAPSL FVNAATWGLM ITGKLVTTNS EAGLSSALTR LIGKGGEPLI RKGVDMAMRL MGEQFVTGET ISEALANSRK YEARGFRYSY DMLGEAATTE EDALRYYASY EQAIHAIGKA AGGRGIYEGP GISIKLSALH ARYSRSQQER TMNELLPRVR SLALLARRYD IGLNIDAEEA DRLELSLDLL EALCFDPELA GWNGIGFVVQ AYQKRCPFVI DYLVDLARRS RHRLMIRLVK GAYWDSEIKR AQVDGLEGYP VYTRKIYTDV SYLACAKKLL AAPDAVYPQF ATHNAYTLAA IYHLAGQNYY PGQYEFQCLH GMGEPLYEEV TGRDKLNRPC RVYAPVGTHE TLLAYLVRRL LENGANTSFV NRIADKTVSV KELVADPVED ALKVVPLGAP HAKIALPREL YGASRVNSMG LDLSNEHRLA SLSSALLASA HHPWRAAPML ADDALAHAAP RDVRNPADLR DVVGTVSEAT PDEVSAALAH AVAAAPIWQA TPVDARADCL ARAADLLEAQ MHTLMGLIVR EAGKSLPNAI AEIREAVDFL RYYSAQIRDE FSNDTHRPLG PVVCISPWNF PLAIFMGQVA AALAAGNTVL AKPAEQTPLI AAQAVRLLRD AGVPAGAVQL LPGNGETVGA ALVADPRTRA VMFTGSTEVA RLINKTLSSR LDPDGRPIPL IAETGGQNAM IVDSSALAEQ VVADVLQSSF DSAGQRCSAL RVLCLQDDVA DRTLTMLKGA MRELALGNPD RLSVDVGPVI DADAKRTIDT HIAAMKDKGH AVTQLEMPDA CAQGTFVPPT IIEIGNVDEL KREVFGPVLH VVRYRRSGLD KLLEQIRATG YGLTLGIHTR IDETIAHVIS RAHVGNIYVN RNVIGAVVGV QPFGGEGLSG TGPKAGGALY LQRLLATRPA GLPRSLEAAL VADAPQEGET RDNPSAALTA LRDWLIEQRE PALAARCDGY LSHVLAGATA VLSGPTGERN TYTLGARGTV LCIAATASGA RAQFAAALAT GNRALFAGAA GEALAAALPA QLKAHAGVRK QADAAFDAVL FEGDSDELLA LVKEVAQRPG PIVSVQGVAA GAFENGGDED YALERLLTER SVSVNTAAAG GNANLMTIG

NT Sequence

atggcaagca ccacactggg cgtcaaagtc gacgatctgc tgcgcgcgcg tctgaaggac gcggcgtcgc ggctcgagcg cactcctcac tggctgatca agcaggcgat cttcgcgtat ctcgagcgga tcgagcatgg tcagcttccg ccggagctct ccggccacac gggcgtcgcc gatctgtccg acagccacgc cgacggcgac gacgacggcg cgccgcaccc gttcctcgaa ttcgcgcaga gcgtgcagcc gcagtcggtg ctgcgcgcgg cgatcacggc cgcgtaccgg cggccggagc ccgaatgcgt gccgttcctg atcggcgagg cgcggctgcc cgcgagcctc gcgtcggacg tgcaggcaat ggcggcgggc ctcgtcgagg cgctgcgcga gaagagctcg ggcggcggcg tcgaggggct gatccatgaa ttctcgctgt cgagccagga gggcgtcgcg ctgatgtgcc tcgccgaggc gctgctgcgg attcccgatc gcgcgacgcg cgacgcgctg attcgcgaca agatcagcaa gggcgactgg cgctcgcacg tcggccacgc gccgtcgctg ttcgtcaacg cggccacctg gggcctgatg attaccggca agctcgtcac gacgaacagc gaggcgggcc tgtcgtcggc gctcacgcgg ctgatcggca agggcggcga gccgttgatc cgaaagggcg tcgacatggc gatgcgcctg atgggcgagc agttcgtcac gggcgagacg atctccgaag cgctcgcgaa cagccgcaag tacgaggcgc gcggcttccg ctactcgtac gacatgctcg gcgaagcggc gaccaccgaa gaggacgcgc tgcgctacta cgcgtcgtac gagcaggcga tccacgcgat cggcaaggcg gcgggcggcc gcggcatcta cgagggcccg ggcatctcga tcaagctctc ggcgctgcat gcgcgctact cgcgctccca gcaggaacgc acgatgaacg agctgctgcc gcgcgtgcgc tcgctcgcgc tgctcgcgcg ccgctatgac atcggcctga acatcgacgc cgaggaggcg gaccggctcg agctctcgct cgatctgctc gaggcgctgt gcttcgatcc ggagctcgcc ggctggaacg gcatcggctt cgtcgtgcag gcgtaccaga agcgctgccc gttcgtgatc gactaccttg tcgacctcgc gcgccgcagc cgccatcgcc tgatgatccg cctcgtgaag ggcgcgtact gggattccga gatcaagcgc gcgcaggtgg acggcctcga aggctatccg gtctatacgc gcaagatcta caccgacgtg tcctacctcg cgtgcgcgaa gaagctgctc gccgcgccgg acgccgtgta tccgcagttc gcgacgcaca acgcgtacac gctcgccgcg atctatcacc tcgcgggcca gaactactac cccggccagt acgagttcca gtgcctgcac gggatgggcg agccgctgta cgaggaagtc accggccgcg acaagctgaa ccgcccatgc cgcgtgtacg cgcccgtcgg cacgcacgag acgctgcttg cgtatctcgt gcgccgcctg ctggagaacg gcgcgaacac gtcgttcgtg aaccggatcg ccgacaagac cgtgtcggtg aaggaactgg tggccgatcc ggtcgaggat gcgctgaagg tcgtgccgct cggcgcgccg cacgcgaaga ttgcgctgcc gcgcgagctg tacggcgcgt cgcgcgtcaa ttcgatgggc ctcgatctgt cgaacgagca ccggctcgcg tcgctgtcgt cggcgctgct cgcgagcgcg catcatccgt ggcgcgccgc gccgatgctc gccgacgacg cgctcgccca cgccgcgccg cgcgacgtgc gcaatccggc cgatctgcgc gacgtcgtcg gcacggtgag cgaagcgacg cccgacgagg tgagcgcggc gctcgcgcac gcggtcgcgg ccgcgccgat ctggcaggcg acgcccgtcg acgcgcgcgc ggactgcctc gcgcgcgcgg cggacctgct cgaagcgcag atgcacacgc tgatgggcct gatcgtgcgc gaggcgggca aatcgctgcc gaacgcgatc gccgagatcc gcgaagcggt cgacttcctg cgctactact cggcgcagat ccgcgacgaa ttctcgaacg acacgcaccg cccgctcggc cccgtcgtct gcatcagccc gtggaacttc ccgctcgcga tcttcatggg ccaggtcgca gccgccctgg cggcgggcaa cacggtgctc gcgaagccgg ccgagcagac gccgctgatc gccgcgcagg cggtgcgcct gctgcgcgac gccggcgtgc cggcgggcgc ggtgcaactg ctgccgggca acggcgagac ggtgggcgcg gcgctcgtgg cggacccccg cacgcgcgcg gtgatgttca ccggctcgac cgaagtcgcg cgcctcatca acaagacgct gtcgtcgcgc ctcgatccgg acggcaggcc gatcccgctg atcgccgaga cgggcggcca gaacgcgatg atcgtcgatt cgtcggcgct cgccgagcag gtggtcgccg acgtgctgca gtcgtcgttc gactcggccg gtcaacgctg ttcggcgctg cgcgttctgt gtctgcagga cgacgtcgcg gatcgcacgc tgacgatgct caagggcgcg atgcgcgagc tcgcgctcgg caatccggac cggctgtcgg tggacgtcgg cccggtgatc gacgccgacg cgaagcgcac gatcgacacg cacatcgcgg cgatgaagga caaggggcac gcggtcacgc agctcgagat gccggacgca tgcgcgcagg gcacgttcgt gccgccgacg atcatcgaga tcggcaacgt cgacgagctc aagcgtgaag tgttcggccc ggtgctgcac gtcgtgcgtt atcgccgcag cggcctcgac aagctgctcg agcagatccg cgcgacgggc tatgggctca cgctcggcat tcacacgcgg atcgacgaga cgatcgcgca cgtgatctcg cgcgcgcacg tcggcaacat ctacgtgaac cgcaacgtga tcggcgcggt ggtgggcgtg cagccgttcg gcggcgaagg gctgtcgggc acggggccga aggcgggcgg cgcgctctac ttgcagcgcc tgctcgcgac gcgcccggcc gggctgccgc gctcgctcga ggcggcgctc gtcgccgatg cgccgcagga aggcgagacg cgcgataatc catccgctgc gctcaccgcg ctgcgcgact ggctgatcga gcagcgcgag ccggcgctcg ccgcgcgctg cgacggctat ctgtcgcacg tgctcgcggg cgcaaccgcc gtgctttcag ggccgacggg cgagcgcaat acgtatacac tcggcgcgcg cggcacggtg ctgtgcatcg cggcgacggc aagcggcgcg cgcgcgcagt tcgcggcggc gctcgccacc ggcaaccgcg cgcttttcgc gggcgcggcg ggcgaggcgc tcgccgcggc gctgccggcg caactgaagg cgcacgcggg cgtgagaaag caggcggacg cggcgttcga cgcggtgctg ttcgaaggcg acagcgacga gttgctcgcg ctcgtgaagg aagtcgcgca gcgcccgggg ccgatcgtgt cggtgcaggg cgtcgcggcg ggcgcgttcg agaacggcgg cgacgaagac tacgcgctcg agcggctcct caccgagcgc tcggtgagcg tgaacaccgc ggcggcaggc ggcaatgcga atttgatgac gattggctga

Details for BupsE.18016.a.A2.GE34337

HARVESTED ON:	1/10/2012
SEQUENCED ON:	1/13/2012
EXPECTED MW:	69kDa
OBSERVED MW:	69kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	77
PERCENT COVERAGE:	63

Validated AA Sequence

Validated NT Sequence

annnnnnnng nnnnntttaa gaaggagata taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg gcaagcacca cactgggcgt caaagtcgac gatctgctgc gcgcgcgtct gaaggacgcg gcgtcgcggc tcgagcgcac tcctcactgg ctgatcaagc aggcgatctt cgcgtatctc gagcggatcg agcatggtca gcttccgccg gagctctccg gccacacggg cgtcgccgat ctgtccgaca gccacgccga cggcgacgac gacggcgcgc cgcacccgtt cctcgaattc gcgcagagcg tgcagccgca gtcggtgctg cgcgcggcga tcacggccgc gtaccggcgg ccggagcccg aatgcgtgcc gttcctgatc ggcgaggcgc ggctgcccgc gagcctcgcg tcggacgtgc aggcaatggc ggcgggcctc gtcgaggcgc tgcgcgagaa gagctcgggc ggcggcgtcg aggggctgat ccatgaattc tcgctgtcga gccaggaggg cgtcgcgctg atgtgcctcg ccgaggcgct gctgcggatt cccgatcgcg cgacgcgcga cgcgctgatt cgcgacaaga tcagcaaggg cgactggcgc tcgcacgtcg gccacgcgcc gtcgctgttc gtcaacgcgg ccacctgggg cctgatgant accggcaagc tcgtcacgac gaacagcgag gcgggcctgt cgtcggcgct cacgcggctg atcggcaagg gcggcgagcc gttganccna aagggcgtcn acatggcgat gcgcctgatg ggcgagcagt tcgtcncggg cgagacganc tccgangcgc tcgcgaacan cnncaagtac naggngcgcg gcttccgcta ctcgtacgan nngctcggcg aancggcgac nancgaagan gacgcgctgc gnntnctacn cgtcgtacga gcaggnnatc ncgnnnncgg nnngnggnng ggnggnnnnn gnatctacna nggnccnggn nnnnnnnatc aagctcgnnn cnncntnnnn ncnnnnnnnn nntcccnnca nnnnnnnnna annnannann nnnnnnnnnn ngnnnnnnnn nnnnnnnnnn nnnncnnnnn ncnnnnnntn nnnnnnnnnn nnnccnnnnn nnnnnnngnn nnnnnnnnnn nannnnnnnn nnnnnt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMASTTLGVK VDDLLRARLK DAASRLERTP HWLIKQAIFA YLERIEHGQL PPELSGHTGV ADLSDSHADG DDDGAPHPFL EFAQSVQPQS VLRAAITAAY RRPEPECVPF LIGEARLPAS LASDVQAMAA GLVEALREKS SGGGVEGLIH EFSLSSQEGV ALMCLAEALL RIPDRATRDA LIRDKISKGD WRSHVGHAPS LFVNAATWGL MITGKLVTTN SEAGLSSALT RLIGKGGEPL IRKGVDMAMR LMGEQFVTGE TISEALANSR KYEARGFRYS YDMLGEAATT EEDALRYYAS YEQAIHAIGK AAGGRGIYEG PGISIKLSAL HARYSRSQQE RTMNELLPRV RSLALLARRY DIGLNIDAEE ADRLELSLDL LEALCFDPEL AGWNGIGFVV QAYQKRCPFV IDYLVDLARR SRHRLMIRLV KGAYWDSEIK RAQVDGLEGY PVYTRKIYTD VSYLACAKKL LAAPDAVYPQ FATHNAYTLA AIYHLAGQNY YPGQYEFQCL HGMGEPLYEE VTGRDKLNRP CRVYAPVGTH ETLLAYLVRR LLENGANTSF VNRIADKTVS VKELVADPVE DALKVVPLGA PHAKIALPRE LYGASRVN

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcaagcacc acactgggcg tcaaagtcga cgatctgctg cgcgcgcgtc tgaaggacgc ggcgtcgcgg ctcgagcgca ctcctcactg gctgatcaag caggcgatct tcgcgtatct cgagcggatc gagcatggtc agcttccgcc ggagctctcc ggccacacgg gcgtcgccga tctgtccgac agccacgccg acggcgacga cgacggcgcg ccgcacccgt tcctcgaatt cgcgcagagc gtgcagccgc agtcggtgct gcgcgcggcg atcacggccg cgtaccggcg gccggagccc gaatgcgtgc cgttcctgat cggcgaggcg cggctgcccg cgagcctcgc gtcggacgtg caggcaatgg cggcgggcct cgtcgaggcg ctgcgcgaga agagctcggg cggcggcgtc gaggggctga tccatgaatt ctcgctgtcg agccaggagg gcgtcgcgct gatgtgcctc gccgaggcgc tgctgcggat tcccgatcgc gcgacgcgcg acgcgctgat tcgcgacaag atcagcaagg gcgactggcg ctcgcacgtc ggccacgcgc cgtcgctgtt cgtcaacgcg gccacctggg gcctgatgat taccggcaag ctcgtcacga cgaacagcga ggcgggcctg tcgtcggcgc tcacgcggct gatcggcaag ggcggcgagc cgttgatccg aaagggcgtc gacatggcga tgcgcctgat gggcgagcag ttcgtcacgg gcgagacgat ctccgaagcg ctcgcgaaca gccgcaagta cgaggcgcgc ggcttccgct actcgtacga catgctcggc gaagcggcga ccaccgaaga ggacgcgctg cgctactacg cgtcgtacga gcaggcgatc cacgcgatcg gcaaggcggc gggcggccgc ggcatctacg agggcccggg catctcgatc aagctctcgg cgctgcatgc gcgctactcg cgctcccagc aggaacgcac gatgaacgag ctgctgccgc gcgtgcgctc gctcgcgctg ctcgcgcgcc gctatgacat cggcctgaac atcgacgccg aggaggcgga ccggctcgag ctctcgctcg atctgctcga ggcgctgtgc ttcgatccgg agctcgccgg ctggaacggc atcggcttcg tcgtgcaggc gtaccagaag cgctgcccgt tcgtgatcga ctaccttgtc gacctcgcgc gccgcagccg ccatcgcctg atgatccgcc tcgtgaaggg cgcgtactgg gattccgaga tcaagcgcgc gcaggtggac ggcctcgaag gctatccggt ctatacgcgc aagatctaca ccgacgtgtc ctacctcgcg tgcgcgaaga agctgctcgc cgcgccggac gccgtgtatc cgcagttcgc gacgcacaac gcgtacacgc tcgccgcgat ctatcacctc gcgggccaga actactaccc cggccagtac gagttccagt gcctgcacgg gatgggcgag ccgctgtacg aggaagtcac cggccgcgac aagctgaacc gcccatgccg cgtgtacgcg cccgtcggca cgcacgagac gctgcttgcg tatctcgtgc gccgcctgct ggagaacggc gcgaacacgt cgttcgtgaa ccggatcgcc gacaagaccg tgtcggtgaa ggaactggtg gccgatccgg tcgaggatgc gctgaaggtc gtgccgctcg gcgcgccgca cgcgaagatt gcgctgccgc gcgagctgta cggcgcgtcg cgcgtcaatt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa

Details for BupsE.18016.a.A2.PW34947

PURIFICATION DATe:	2/9/2012
CONCENTRATION:	34.68mg/ml
OBSERVED MW:	70kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	200µl
PERCENT IDENTITY:	77
PERCENT COVERAGE:	63

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCAAGCACC ACACTGGGCG TCAAAGTCGA CGATCTGCTG CGCGCGCGTC TGAAGGACGC GGCGTCGCGG CTCGAGCGCA CTCCTCACTG GCTGATCAAG CAGGCGATCT TCGCGTATCT CGAGCGGATC GAGCATGGTC AGCTTCCGCC GGAGCTCTCC GGCCACACGG GCGTCGCCGA TCTGTCCGAC AGCCACGCCG ACGGCGACGA CGACGGCGCG CCGCACCCGT TCCTCGAATT CGCGCAGAGC GTGCAGCCGC AGTCGGTGCT GCGCGCGGCG ATCACGGCCG CGTACCGGCG GCCGGAGCCC GAATGCGTGC CGTTCCTGAT CGGCGAGGCG CGGCTGCCCG CGAGCCTCGC GTCGGACGTG CAGGCAATGG CGGCGGGCCT CGTCGAGGCG CTGCGCGAGA AGAGCTCGGG CGGCGGCGTC GAGGGGCTGA TCCATGAATT CTCGCTGTCG AGCCAGGAGG GCGTCGCGCT GATGTGCCTC GCCGAGGCGC TGCTGCGGAT TCCCGATCGC GCGACGCGCG ACGCGCTGAT TCGCGACAAG ATCAGCAAGG GCGACTGGCG CTCGCACGTC GGCCACGCGC CGTCGCTGTT CGTCAACGCG GCCACCTGGG GCCTGATGAT TACCGGCAAG CTCGTCACGA CGAACAGCGA GGCGGGCCTG TCGTCGGCGC TCACGCGGCT GATCGGCAAG GGCGGCGAGC CGTTGATCCG AAAGGGCGTC GACATGGCGA TGCGCCTGAT GGGCGAGCAG TTCGTCACGG GCGAGACGAT CTCCGAAGCG CTCGCGAACA GCCGCAAGTA CGAGGCGCGC GGCTTCCGCT ACTCGTACGA CATGCTCGGC GAAGCGGCGA CCACCGAAGA GGACGCGCTG CGCTACTACG CGTCGTACGA GCAGGCGATC CACGCGATCG GCAAGGCGGC GGGCGGCCGC GGCATCTACG AGGGCCCGGG CATCTCGATC AAGCTCTCGG CGCTGCATGC GCGCTACTCG CGCTCCCAGC AGGAACGCAC GATGAACGAG CTGCTGCCGC GCGTGCGCTC GCTCGCGCTG CTCGCGCGCC GCTATGACAT CGGCCTGAAC ATCGACGCCG AGGAGGCGGA CCGGCTCGAG CTCTCGCTCG ATCTGCTCGA GGCGCTGTGC TTCGATCCGG AGCTCGCCGG CTGGAACGGC ATCGGCTTCG TCGTGCAGGC GTACCAGAAG CGCTGCCCGT TCGTGATCGA CTACCTTGTC GACCTCGCGC GCCGCAGCCG CCATCGCCTG ATGATCCGCC TCGTGAAGGG CGCGTACTGG GATTCCGAGA TCAAGCGCGC GCAGGTGGAC GGCCTCGAAG GCTATCCGGT CTATACGCGC AAGATCTACA CCGACGTGTC CTACCTCGCG TGCGCGAAGA AGCTGCTCGC CGCGCCGGAC GCCGTGTATC CGCAGTTCGC GACGCACAAC GCGTACACGC TCGCCGCGAT CTATCACCTC GCGGGCCAGA ACTACTACCC CGGCCAGTAC GAGTTCCAGT GCCTGCACGG GATGGGCGAG CCGCTGTACG AGGAAGTCAC CGGCCGCGAC AAGCTGAACC GCCCATGCCG CGTGTACGCG CCCGTCGGCA CGCACGAGAC GCTGCTTGCG TATCTCGTGC GCCGCCTGCT GGAGAACGGC GCGAACACGT CGTTCGTGAA CCGGATCGCC GACAAGACCG TGTCGGTGAA GGAACTGGTG GCCGATCCGG TCGAGGATGC GCTGAAGGTC GTGCCGCTCG GCGCGCCGCA CGCGAAGATT GCGCTGCCGC GCGAGCTGTA CGGCGCGTCG CGCGTCAATT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA