SSGCID: Target Page

BupsE.17935.b

60 kDa chaperonin 1 (GroEL protein 1) (Protein Cpn60 1)

CENTER ID:	BupsE.17935.b
ORGANISM:	Burkholderia pseudomallei K96243
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsE.17935.b.A1.GE34340	full length		1	546

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsE.17935.b.A1.PW34950	full length		1	546

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272560.6.peg.3072
RefSeq:	YP_109293.1
UniProt:	Q9F712

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAAKDVVFGD SARAKMVEGV NILANAVKVT LGPKGRNVVL ERSFGGPTVT KDGVSVAKEI ELKDKLQNMG AQMVKEVASK TSDNAGDGTT TATVLAQSIV REGMKYVASG MNPMDLKRGI DKAVAAAVEE LKKISKPCTT NKEIAQVGAI SANSDSSIGD RIAEAMDKVG KEGVITVEDG KSLADELDVV EGMQFDRGYL SPYFINNPDK QVAVLENPFV LLHDKKVSNI RDLLPVLEQV AKAGRPLLII AEDVEGEALA TLVVNNIRGI LKTVAVKAPG FGDRRKAMLE DIAILTGGQV IAEETGLTLE KATLAELGQA KRIEVGKENT TIIDGAGEAV NIEARVKQIR TQIEEATSDY DREKLQERVA KLAGGVAVIK VGAATEVEMK EKKARVEDAL HATRAAVEEG IVPGGGVALI RARTAIAGLT GVNADQNAGI KIVLRAMEEP LRQIVTNGGE EASVVVAAVA AGKGNYGYNA ATGEYVDMVE AGVVDPTKVT RTALQNAASV AGLLLTTDAA VAELPKEDAP MPGGMPGGMG GMGMDM

NT Sequence

atggcagcta aagacgtcgt attcggcgat tccgcacgcg cgaagatggt cgaaggcgtg aacattctcg ccaacgctgt gaaggtcacg ctgggtccga agggccgcaa cgtggtgctc gagcgcagct tcggcggccc gacggtcacg aaggacggtg tgtcggtcgc gaaggaaatc gagctgaagg acaagctcca gaacatgggc gcgcagatgg tcaaggaagt cgcttccaag accagcgaca acgccggcga cggcacgacg acggccaccg tcctcgcgca atcgatcgtc cgcgaaggca tgaagtacgt cgcatcgggc atgaacccga tggacctgaa gcgcggcatc gacaaggcag tcgccgcggc agtcgaagag ctgaagaaga tcagcaagcc gtgcacgacg aacaaggaaa tcgcgcaagt cggcgcgatc tcggcgaaca gcgattcgtc gatcggcgat cgcatcgctg aagcgatgga caaggtcggc aaggaaggcg tgatcaccgt cgaagacggc aagtcgctcg ccgacgagct cgacgtcgtc gaaggcatgc agttcgaccg cggctacctg tcgccgtact tcatcaacaa cccggacaag caagtcgccg tcctcgagaa cccgttcgtg ctgctgcacg acaagaaggt gtcgaacatc cgcgacctgt tgccggtgct cgagcaagtc gcgaaggctg gccgtccgct gctgatcatc gccgaagacg tcgaaggcga agcgctcgca acgctggtcg tcaacaacat ccgcggcatc ctgaagaccg ttgcggtcaa ggcgccgggc ttcggcgatc gtcgcaaggc gatgctggaa gacatcgcga tcctgacggg cggccaggtc atcgcggaag aaaccggcct cacgctcgag aaggcaacgc tggcagaact gggccaggcg aagcgcatcg aagtgggcaa ggaaaacacg acgatcatcg acggcgcggg cgaagccgtg aacatcgaag cgcgcgtcaa gcaaatccgc acgcaaatcg aagaagcgac atcggactac gaccgtgaaa agctgcaaga gcgcgtggcc aagctggcag gcggcgtggc ggtgatcaag gttggcgctg cgaccgaagt cgaaatgaag gaaaagaagg cacgtgtcga ggacgcgctg cacgccaccc gcgctgccgt tgaagaaggc atcgtcccgg gcggcggcgt cgcgctgatc cgcgcacgca ccgcgatcgc gggcctgacc ggcgtgaacg ccgaccagaa cgccggcatc aagatcgtgc tgcgcgcgat ggaagagccg ctgcgccaga tcgtcacgaa cggcggcgaa gaagcgagcg tcgtggtggc ggcagttgct gcgggcaagg gcaactacgg ctacaacgcg gcgacgggcg agtacgtcga catggtcgaa gccggcgtcg tcgatccgac gaaggtcacc cgtaccgcgc tgcagaacgc ggcttcggtc gccggcctgc tgctgacgac ggacgcagcc gttgccgaac tgccgaagga agacgctccg atgccgggcg gcatgccggg cggcatgggc ggcatgggca tggacatgta a

Details for BupsE.17935.b.A1.GE34340

HARVESTED ON:	1/10/2012
SEQUENCED ON:	1/17/2012
EXPECTED MW:	59kDa
OBSERVED MW:	64kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	91

Validated AA Sequence

MGTLEAQTQG PGSMAAKDVV FGDSARAKMV EGVNILANAV KVTLGPKGRN VVLERSFGGP TVTKDGVSVA KEIELKDKLQ NMGAQMVKEV ASKTSDNAGD GTTTATVLAQ SIVREGMKYV ASGMNPMDLK RGIDKAVAAA VEELKKISKP CTTNKEIAQV GAISANSDSS IGDRIAEAMD KVGKEGVITV EDGKSLADEL DVVEGMQFDR GYLSPYFINN PDKQVAVLEN PFVLLHDKKV SNIRDLLXVL EQVAKAGRPL LIIAXDVEGE ALATLVVNNI RGILKTVAVK APGFGDRRKA MLEDIAIXTG GQVIAEETXX TLEKATLAEL GQAKRIEVGK ENTTIXDGAX EAVNIEARVK QIRTQIEEAT SDYDREKLQE RVAKLAGGVA VIKVGAATEV EMKEKKARVE DALHATRAAV EEGIVPGGGV ALIRARTAIA GLTGVNADQN AGIKIVLRAM EEPLRQIVTN GGEEASVVVA AVAAGKGNYG YNAATGEYVD MVEAGVVDPT KVTRTA

Validated NT Sequence

tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggcagc taaagacgtc gtattcggcg attccgcacg cgcgaagatg gtcgaaggcg tgaacattct cgccaacgct gtgaaggtca cgctgggtcc gaagggccgc aacgtggtgc tcgagcgcag cttcggcggc ccgacggtca cgaaggacgg tgtgtcggtc gcgaaggaaa tcgagctgaa ggacaagctc cagaacatgg gcgcgcagat ggtcaaggaa gtcgcttcca agaccagcga caacgccggc gacggcacga cgacggccac cgtcctcgcg caatcgatcg tccgcgaagg catgaagtac gtcgcatcgg gcatgaaccc gatggacctg aagcgcggca tcgacaaggc agtcgccgcg gcagtcgaag agctgaagaa gatcagcaag ccgtgcacga cgaacaagga aatcgcgcaa gtcggcgcga tctcggcgaa cagcgattcg tcgatcggcg atcgcatcgc tgaagcgatg gacaaggtcg gcaaggaagg cgtgatcacc gtcgaagacg gcaagtcgct cgccgacgag ctcgacgtcg tcgaaggcat gcagttcgac cgcggctacc tgtcgccgta cttcatcaac aacccggaca agcaagtcgc cgtcctcgag aacccgttcg tgctgctgca cgacaagaag gtgtcgaaca tccgcgacct gttgncggtg ctcgagcaag tcgcgaaggc tggccgtccn ctgctgatca tcgccnanga cgtcgaaggc gaagcgctcg caacgctggt cgtcaacaac atccgcggca tcctgaagac cgttgcggtc aaggcgccgg gcttcggcga tcgtcgcaag gcgatgctgg aagacatcgc gatcnngacg ggcggccagg tcatcgcgga agaaaccgnc ntcacgctcg agaaggcaac gctggcagaa ctgggccagg cgaagcgcat cgaagtgggc aaggaaaaca cgacgatcat ngacggcgcg gnngaagccg tgaacatcga agcgcgcgtc aagcaaatcc gcacgcaaat cgaagaagcg acatcggact acgaccgtga aaagctgcaa gagcgcgtgg ccaagctngc aggcggcgtg gcggtgatca aggtnggcgc tgcgaccgaa gtcgaaatga aggaaaagaa ggcacgtgtc gaggacgcgc tgcacgccac ccgcgctgcc gttgaagaag gcatcgtccc gggcggcggc gtcgcgctga tccgcgcacg caccgcgatc gcgggcctga ccggcgtgaa cgccgaccag aacgccggca tcaagatcgt gctgcgcgcg atggaagagc cgctgcgcca gatcgtcacg aacggcggcg aagaagcgag cgtcgtggtg gcggcagttg ctgcgggcaa gggcaactac ggctacaacg cggcgacggg cgagtacgtc gacatggtcg aagccggcgt cgtcgatccg acgaaggtca cccgtaccgc gct

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAAKDVVFG DSARAKMVEG VNILANAVKV TLGPKGRNVV LERSFGGPTV TKDGVSVAKE IELKDKLQNM GAQMVKEVAS KTSDNAGDGT TTATVLAQSI VREGMKYVAS GMNPMDLKRG IDKAVAAAVE ELKKISKPCT TNKEIAQVGA ISANSDSSIG DRIAEAMDKV GKEGVITVED GKSLADELDV VEGMQFDRGY LSPYFINNPD KQVAVLENPF VLLHDKKVSN IRDLLPVLEQ VAKAGRPLLI IAEDVEGEAL ATLVVNNIRG ILKTVAVKAP GFGDRRKAML EDIAILTGGQ VIAEETGLTL EKATLAELGQ AKRIEVGKEN TTIIDGAGEA VNIEARVKQI RTQIEEATSD YDREKLQERV AKLAGGVAVI KVGAATEVEM KEKKARVEDA LHATRAAVEE GIVPGGGVAL IRARTAIAGL TGVNADQNAG IKIVLRAMEE PLRQIVTNGG EEASVVVAAV AAGKGNYGYN AATGEYVDMV EAGVVDPTKV TRTALQNAAS VAGLLLTTDA AVAELPKEDA PMPGGMPGGM GGMGMDM

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcagctaaa gacgtcgtat tcggcgattc cgcacgcgcg aagatggtcg aaggcgtgaa cattctcgcc aacgctgtga aggtcacgct gggtccgaag ggccgcaacg tggtgctcga gcgcagcttc ggcggcccga cggtcacgaa ggacggtgtg tcggtcgcga aggaaatcga gctgaaggac aagctccaga acatgggcgc gcagatggtc aaggaagtcg cttccaagac cagcgacaac gccggcgacg gcacgacgac ggccaccgtc ctcgcgcaat cgatcgtccg cgaaggcatg aagtacgtcg catcgggcat gaacccgatg gacctgaagc gcggcatcga caaggcagtc gccgcggcag tcgaagagct gaagaagatc agcaagccgt gcacgacgaa caaggaaatc gcgcaagtcg gcgcgatctc ggcgaacagc gattcgtcga tcggcgatcg catcgctgaa gcgatggaca aggtcggcaa ggaaggcgtg atcaccgtcg aagacggcaa gtcgctcgcc gacgagctcg acgtcgtcga aggcatgcag ttcgaccgcg gctacctgtc gccgtacttc atcaacaacc cggacaagca agtcgccgtc ctcgagaacc cgttcgtgct gctgcacgac aagaaggtgt cgaacatccg cgacctgttg ccggtgctcg agcaagtcgc gaaggctggc cgtccgctgc tgatcatcgc cgaagacgtc gaaggcgaag cgctcgcaac gctggtcgtc aacaacatcc gcggcatcct gaagaccgtt gcggtcaagg cgccgggctt cggcgatcgt cgcaaggcga tgctggaaga catcgcgatc ctgacgggcg gccaggtcat cgcggaagaa accggcctca cgctcgagaa ggcaacgctg gcagaactgg gccaggcgaa gcgcatcgaa gtgggcaagg aaaacacgac gatcatcgac ggcgcgggcg aagccgtgaa catcgaagcg cgcgtcaagc aaatccgcac gcaaatcgaa gaagcgacat cggactacga ccgtgaaaag ctgcaagagc gcgtggccaa gctggcaggc ggcgtggcgg tgatcaaggt tggcgctgcg accgaagtcg aaatgaagga aaagaaggca cgtgtcgagg acgcgctgca cgccacccgc gctgccgttg aagaaggcat cgtcccgggc ggcggcgtcg cgctgatccg cgcacgcacc gcgatcgcgg gcctgaccgg cgtgaacgcc gaccagaacg ccggcatcaa gatcgtgctg cgcgcgatgg aagagccgct gcgccagatc gtcacgaacg gcggcgaaga agcgagcgtc gtggtggcgg cagttgctgc gggcaagggc aactacggct acaacgcggc gacgggcgag tacgtcgaca tggtcgaagc cggcgtcgtc gatccgacga aggtcacccg taccgcgctg cagaacgcgg cttcggtcgc cggcctgctg ctgacgacgg acgcagccgt tgccgaactg ccgaaggaag acgctccgat gccgggcggc atgccgggcg gcatgggcgg catgggcatg gacatgtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for BupsE.17935.b.A1.PW34950

PURIFICATION DATe:	3/16/2012
CONCENTRATION:	20.17mg/ml
OBSERVED MW:	56kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	11
VIAL VOLUME:	110µl
PERCENT IDENTITY:	98
PERCENT COVERAGE:	91

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCAGCTAAA GACGTCGTAT TCGGCGATTC CGCACGCGCG AAGATGGTCG AAGGCGTGAA CATTCTCGCC AACGCTGTGA AGGTCACGCT GGGTCCGAAG GGCCGCAACG TGGTGCTCGA GCGCAGCTTC GGCGGCCCGA CGGTCACGAA GGACGGTGTG TCGGTCGCGA AGGAAATCGA GCTGAAGGAC AAGCTCCAGA ACATGGGCGC GCAGATGGTC AAGGAAGTCG CTTCCAAGAC CAGCGACAAC GCCGGCGACG GCACGACGAC GGCCACCGTC CTCGCGCAAT CGATCGTCCG CGAAGGCATG AAGTACGTCG CATCGGGCAT GAACCCGATG GACCTGAAGC GCGGCATCGA CAAGGCAGTC GCCGCGGCAG TCGAAGAGCT GAAGAAGATC AGCAAGCCGT GCACGACGAA CAAGGAAATC GCGCAAGTCG GCGCGATCTC GGCGAACAGC GATTCGTCGA TCGGCGATCG CATCGCTGAA GCGATGGACA AGGTCGGCAA GGAAGGCGTG ATCACCGTCG AAGACGGCAA GTCGCTCGCC GACGAGCTCG ACGTCGTCGA AGGCATGCAG TTCGACCGCG GCTACCTGTC GCCGTACTTC ATCAACAACC CGGACAAGCA AGTCGCCGTC CTCGAGAACC CGTTCGTGCT GCTGCACGAC AAGAAGGTGT CGAACATCCG CGACCTGTTG CCGGTGCTCG AGCAAGTCGC GAAGGCTGGC CGTCCGCTGC TGATCATCGC CGAAGACGTC GAAGGCGAAG CGCTCGCAAC GCTGGTCGTC AACAACATCC GCGGCATCCT GAAGACCGTT GCGGTCAAGG CGCCGGGCTT CGGCGATCGT CGCAAGGCGA TGCTGGAAGA CATCGCGATC CTGACGGGCG GCCAGGTCAT CGCGGAAGAA ACCGGCCTCA CGCTCGAGAA GGCAACGCTG GCAGAACTGG GCCAGGCGAA GCGCATCGAA GTGGGCAAGG AAAACACGAC GATCATCGAC GGCGCGGGCG AAGCCGTGAA CATCGAAGCG CGCGTCAAGC AAATCCGCAC GCAAATCGAA GAAGCGACAT CGGACTACGA CCGTGAAAAG CTGCAAGAGC GCGTGGCCAA GCTGGCAGGC GGCGTGGCGG TGATCAAGGT TGGCGCTGCG ACCGAAGTCG AAATGAAGGA AAAGAAGGCA CGTGTCGAGG ACGCGCTGCA CGCCACCCGC GCTGCCGTTG AAGAAGGCAT CGTCCCGGGC GGCGGCGTCG CGCTGATCCG CGCACGCACC GCGATCGCGG GCCTGACCGG CGTGAACGCC GACCAGAACG CCGGCATCAA GATCGTGCTG CGCGCGATGG AAGAGCCGCT GCGCCAGATC GTCACGAACG GCGGCGAAGA AGCGAGCGTC GTGGTGGCGG CAGTTGCTGC GGGCAAGGGC AACTACGGCT ACAACGCGGC GACGGGCGAG TACGTCGACA TGGTCGAAGC CGGCGTCGTC GATCCGACGA AGGTCACCCG TACCGCGCTG CAGAACGCGG CTTCGGTCGC CGGCCTGCTG CTGACGACGG ACGCAGCCGT TGCCGAACTG CCGAAGGAAG ACGCTCCGAT GCCGGGCGGC ATGCCGGGCG GCATGGGCGG CATGGGCATG GACATGTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA