BupsE.17371.a

Putative exported protein (BPSS0344)

CENTER ID: BupsE.17371.a
ORGANISM: Burkholderia pseudomallei K96243
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsE.17371.a.B2.GE42225 mature protein 25 360
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272560.51.peg.6464
UniProt: Q63NF5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTYTNFPRGG AWRRFALAAG AAALLQGAAA QAQAAAVVLY TADGLENLYR DVLPAFEKQE GVKVNIVTAG SGEVVNRANV EKGSPKADVI VTLPPFIQQA GQFGLLQPYR SVNYKNVPAI AKAEDGSWAT FVNNYFSFAI NPSVVKSQPK TFADLLHPDY SGKLAYSNPA TAGDGMAVII LTSALMGEDK AFDYLAKLER SVKFHTKGTG YLNVLLSRNE IAVANGDLQM DLDDAEHGGL SIKPIFVAAK AGEPPTTFQL PYAIGLVKGG PNQDAGRKLI DYLMSADVQA KVPDMFGIPG RTDVPLSGKN GEAVKRAIAG VKLIPVDWDA VMAKKPVWTE RWKKEVIGDS GKQTEVVKPK
NT Sequence
atgacgtata cgaatttccc gcgcggcggc gcctggcgcc gcttcgcgct cgccgccggc gccgccgcgc tgttgcaagg cgcggcagcg caggcgcagg cggcagccgt cgtgctgtac acggcggacg gcctcgagaa cctgtaccgc gacgtgctgc ccgcgttcga gaagcaggaa ggcgtgaagg tgaacatcgt gacggcgggc agcggcgaag tggtgaaccg cgcgaacgtc gagaagggct cgccgaaggc cgacgtgatc gtcacgctgc cgccgttcat tcagcaggcc ggccagttcg gcctgctgca gccgtaccgc agcgtcaact acaagaacgt gccggcgatc gcgaaggcgg aagacggctc atgggcgacg ttcgtcaaca actacttctc gttcgcgatc aacccgtcgg tcgtgaagag ccagccgaag acgttcgccg atctgctgca tcccgattac agcggcaagc tcgcgtattc gaacccggcg acggcgggcg acgggatggc cgtcatcatc ctgacgagcg cgctgatggg cgaggacaag gcgttcgact atctcgcgaa gctcgagcgc agcgtgaagt tccacacgaa gggcacgggc tacctgaacg tgctgctgtc gcgcaacgag atcgcggtcg cgaacggcga tctgcagatg gatctggacg acgccgagca cggcggcctg tcgatcaagc cgatcttcgt cgccgcgaag gcgggcgagc cgccgacgac gttccagctg ccgtacgcga tcggcctcgt caagggcggc ccgaaccagg acgcgggcag gaagctgatc gactacctga tgtcggccga cgtgcaggcg aaggtgcccg acatgttcgg cattccgggc cgcaccgacg tgccgctttc gggcaagaac ggcgaggcgg tgaagcgcgc gatcgccggc gtgaagctga ttccggtcga ctgggacgcg gtgatggcga agaagcccgt gtggaccgag cgctggaaga aggaagtgat cggcgattcg ggcaagcaga ccgaagtcgt caagccgaaa tga
Details for BupsE.17371.a.B2.GE42225
HARVESTED ON: 2/23/2018
SEQUENCED ON: 3/1/2018
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHML QGAAAQAQAA AVVLYTADGL ENLYRDVLPA FEKQEGVKVN IVTAGSGEVV NRANVEKGSP KADVIVTLPP FIQQAGQFGL LQPYRSVNYK NVPAIAKAED GSWATFVNNY FSFAINPSVV KSQPKTFADL LHPDYSGKLA YSNPATAGDG MAVIILTSAL MGEDKAFDYL AKLERSVKFH TKGTGYLNVL LSRNEIAVAN GDLQMDLDDA EHGGLSIKPI FVAAKAGEPP TTFQLPYAIG LVKGGPNQDA GRKLIDYLMS ADVQAKVPDM FGIPGRTDVP LSGKNGEAVK RAIAGVKLIP VDWDAVMAKK PVWTERWKKE VIGDSGK
Validated NT Sequence
ctgcttgccc gaatcgccga tcacttcctt cttccagcgc tcggtccaca cgggcttctt cgccatcacc gcgtcccagt cgaccggaat cagcttcacg ccggcgatcg cgcgcttcac cgcctcgccg ttcttgcccg aaagcggcac gtcggtgcgg cccggaatgc cgaacatgtc gggcaccttc gcctgcacgt cggccgacat caggtagtcg atcagcttcc tgcccgcgtc ctggttcggg ccgcccttga cgaggccgat cgcgtacggc agctggaacg tcgtcggcgg ctcgcccgcc ttcgcggcga cgaagatcgg cttgatcgac aggccgccgt gctcggcgtc gtccagatcc atctgcagat cgccgttcgc gaccgcgatc tcgttgcgcg acagcagcac gttcaggtag cccgtgccct tcgtgtggaa cttcacgctg cgctcgagct tcgcgagata gtcgaacgcc ttgtcctcgc ccatcagcgc gctcgtcagg atgatgacgg ccatcccgtc gcccgccgtc gccgggttcg aatacgcgag cttgccgctg taatcgggat gcagcagatc ggcgaacgtc ttcggctggc tcttcacgac cgacgggttg atcgcgaacg agaagtagtt gttgacgaac gtcgcccatg agccgtcttc cgccttcgcg atcgccggca cgttcttgta gttgacgctg cggtacggct gcagcaggcc gaactggccg gcctgctgaa tgaacggcgg cagcgtgacg atcacgtcgg ccttcggcga gcccttctcg acgttcgcgc ggttcaccac ttcgccgctg cccgccgtca cgatgttcac cttcacgcct tcctgcttct cgaacgcggg cagcacgtcg cggtacaggt tctcgaggcc gtccgccgtg tacagcacga cggctgccgc ctgcgcctgc gctgccgcgc cttgcaacat atggtggtgg tggtggtgag ccatggtata
Expected Protein Sequence
MAHHHHHHLQ GAAAQAQAAA VVLYTADGLE NLYRDVLPAF EKQEGVKVNI VTAGSGEVVN RANVEKGSPK ADVIVTLPPF IQQAGQFGLL QPYRSVNYKN VPAIAKAEDG SWATFVNNYF SFAINPSVVK SQPKTFADLL HPDYSGKLAY SNPATAGDGM AVIILTSALM GEDKAFDYLA KLERSVKFHT KGTGYLNVLL SRNEIAVANG DLQMDLDDAE HGGLSIKPIF VAAKAGEPPT TFQLPYAIGL VKGGPNQDAG RKLIDYLMSA DVQAKVPDMF GIPGRTDVPL SGKNGEAVKR AIAGVKLIPV DWDAVMAKKP VWTERWKKEV IGDSGKQTEV VKPK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgcaag gcgcggcagc gcaggcgcag gcggcagccg tcgtgctgta cacggcggac ggcctcgaga acctgtaccg cgacgtgctg cccgcgttcg agaagcagga aggcgtgaag gtgaacatcg tgacggcggg cagcggcgaa gtggtgaacc gcgcgaacgt cgagaagggc tcgccgaagg ccgacgtgat cgtcacgctg ccgccgttca ttcagcaggc cggccagttc ggcctgctgc agccgtaccg cagcgtcaac tacaagaacg tgccggcgat cgcgaaggcg gaagacggct catgggcgac gttcgtcaac aactacttct cgttcgcgat caacccgtcg gtcgtgaaga gccagccgaa gacgttcgcc gatctgctgc atcccgatta cagcggcaag ctcgcgtatt cgaacccggc gacggcgggc gacgggatgg ccgtcatcat cctgacgagc gcgctgatgg gcgaggacaa ggcgttcgac tatctcgcga agctcgagcg cagcgtgaag ttccacacga agggcacggg ctacctgaac gtgctgctgt cgcgcaacga gatcgcggtc gcgaacggcg atctgcagat ggatctggac gacgccgagc acggcggcct gtcgatcaag ccgatcttcg tcgccgcgaa ggcgggcgag ccgccgacga cgttccagct gccgtacgcg atcggcctcg tcaagggcgg cccgaaccag gacgcgggca ggaagctgat cgactacctg atgtcggccg acgtgcaggc gaaggtgccc gacatgttcg gcattccggg ccgcaccgac gtgccgcttt cgggcaagaa cggcgaggcg gtgaagcgcg cgatcgccgg cgtgaagctg attccggtcg actgggacgc ggtgatggcg aagaagcccg tgtggaccga gcgctggaag aaggaagtga tcggcgattc gggcaagcag accgaagtcg tcaagccgaa atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat