BupsE.10611.a

Zinc-binding dehydrogenase

CENTER ID: BupsE.10611.a
ORGANISM: Burkholderia pseudomallei K96243
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsE.10611.a.A2.GE34344 domain 1 1 191
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272560.6.peg.4931
RefSeq: YP_110851.1
UniProt: Q63M20

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKALVYHGPG RKSLDERPMP ELAAPTDVIV RVTRTTICGT DLHILKGDVP SCEPGRVLGH EGVGVVHRAG GAVSTFKEGD RVLISCISSC GQCEYCRRGM YSHCATGGWI LGNRIDGTQA EYVRIPHAQT SLYRIAPGAD EEALVMLSDI LPTGFECGVL NGRVQPGGTV AIIGAGPIGL AALLTAQFYS PAQIVMIDPD PNRLALARRF GATACIDNGQ VDAVAGVKAL TGDGVDCAIE AVGIPASFEL CQRIIAPGGV IANVGVHGMK ADLHLETLWD RNITITTRLV DTSSTPMLLK TVGAGRLDPK QLITHRFSLA DIGQAYETFG HAAHTRALKV IVEVSE
NT Sequence
atgaaagcgc tggtgtatca cgggccgggc cgcaagtcgc tggacgagcg cccgatgccc gagctggccg cgccgacgga cgtcatcgtg cgcgtcacgc gcacgacgat ctgcggcacc gatctgcaca tcctgaaggg cgacgtgccg agctgcgagc cggggcgcgt gctcggccac gagggcgtgg gcgtcgtgca tcgcgcgggc ggcgcggtga gcacgttcaa ggagggcgac cgcgtgctga tctcgtgcat ttcgtcgtgc gggcagtgcg aatactgccg gcgcgggatg tattcgcact gcgcgacggg cggctggatt ctcggcaacc ggatcgacgg cacgcaggcc gaatacgtgc ggattccgca cgcgcagacg agcctgtacc ggatcgcgcc cggcgccgac gaggaggcgc tcgtgatgct gtccgacatc ctgccgacgg gcttcgagtg cggcgtgctg aacggccgcg tgcagccggg cggcacggtc gcgatcatcg gcgcggggcc gatcgggctc gcggcgctgt tgaccgcgca gttctattcg cccgcgcaga tcgtgatgat cgatccggac ccgaaccggc tcgcgctcgc gcggcgcttc ggcgcgaccg cgtgcatcga caacggccag gtcgacgcgg tggccggcgt gaaggcgttg acgggcgacg gcgtggattg cgcgatcgag gcagtcggca ttccggcgtc attcgagctg tgccagcgga tcatcgcgcc gggcggcgtg atcgccaacg tcggcgtgca cggcatgaag gcggatctgc atctcgagac gctgtgggac cgtaacatca cgatcacgac ccggctcgtc gacacgtcga gcacgccgat gctgctgaag accgtcggcg cgggacggct cgacccgaag caactgatca cgcaccgttt ttcgctcgcg gatatcgggc aggcgtacga gacgttcggt catgcggcgc acacgcgcgc gctgaaggtc atcgtcgagg tgtccgagta g
Details for BupsE.10611.a.A2.GE34344
HARVESTED ON: 1/10/2012
SEQUENCED ON: 1/25/2012
EXPECTED MW: 22kDa
OBSERVED MW: 22kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 94
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMXAXVXXXX XXKSLDERPM PELAAPTDVI VRVTRTTICG TDLHILKGDV PSCEPGRVLG HEGVGVXXRA GGAVSTFKEG DRVLISCISS XGQCEYCRRG MYSHCATGGW ILGNRIDGTQ AEYVRIPHAQ TSLYRIAPGA DEEALVMLSD ILPTGFECGV LNGRVQPGGT VAIIGAGPIG LAALLTAQFY SP
Validated NT Sequence
ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgnnngcn cnngtgnntn nnannnnnnn nnncaagtcg ctggacgagc gcccgatgcc cgagctggcc gcgccgacgg acgtcatcgt gcgcgtcacg cgcacgacga tctgcggcac cgatctgcac atcctgaagg gcgacgtgcc gagctgcgag ccggggcgcg tgctcggcca cgagggcgtg ggcgtnnngn ntcgcgcggg cggcgcggtg agcacgttca aggagggcga ccgcgtgctg atctcgtgca tttcgtcgng ngggcagtgc gaatactgcc ggcgcgggat gtattcgcac tgcgcgacgg gcggctggat tctcggcaac cggatcgacg gcacgcaggc cgaatacgtg cggattccgc acgcgcagac gagcctgtac cggatcgcgc ccggcgccga cgaggaggcg ctcgtgatgc tgtccgacat cctgccgacg ggcttcgagt gcggcgtgct gaacggccgc gtgcagccgg gcggcacggt cgcgatcatc ggcgcggggc cgatcgggct cgcggcgctg ttgaccgcgc agttctattc gccctaataa acagcacgaa caagttctgc agccnagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgncaccnnt nannnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKALVYHGP GRKSLDERPM PELAAPTDVI VRVTRTTICG TDLHILKGDV PSCEPGRVLG HEGVGVVHRA GGAVSTFKEG DRVLISCISS CGQCEYCRRG MYSHCATGGW ILGNRIDGTQ AEYVRIPHAQ TSLYRIAPGA DEEALVMLSD ILPTGFECGV LNGRVQPGGT VAIIGAGPIG LAALLTAQFY SP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaagcgctg gtgtatcacg ggccgggccg caagtcgctg gacgagcgcc cgatgcccga gctggccgcg ccgacggacg tcatcgtgcg cgtcacgcgc acgacgatct gcggcaccga tctgcacatc ctgaagggcg acgtgccgag ctgcgagccg gggcgcgtgc tcggccacga gggcgtgggc gtcgtgcatc gcgcgggcgg cgcggtgagc acgttcaagg agggcgaccg cgtgctgatc tcgtgcattt cgtcgtgcgg gcagtgcgaa tactgccggc gcgggatgta ttcgcactgc gcgacgggcg gctggattct cggcaaccgg atcgacggca cgcaggccga atacgtgcgg attccgcacg cgcagacgag cctgtaccgg atcgcgcccg gcgccgacga ggaggcgctc gtgatgctgt ccgacatcct gccgacgggc ttcgagtgcg gcgtgctgaa cggccgcgtg cagccgggcg gcacggtcgc gatcatcggc gcggggccga tcgggctcgc ggcgctgttg accgcgcagt tctattcgcc ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa