SSGCID: Target Page

BupsE.01673.a

Outer membrane efflux protein (oprM BPSS0734)

CENTER ID:	BupsE.01673.a
ORGANISM:	Burkholderia pseudomallei K96243
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsE.01673.a.B2.GE42222	mature protein		28	619

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsE.01673.a.B2.PW38474	mature protein		28	619

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272560.51.peg.6962
UniProt:	Q63MC6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MHSPPFLSSP KRALPIALAL SLAGCSMEPA YQRPDAPMPS AYPNGPAYAT PGAPRANPGE PAAAELGWRN FLADAQLQQL VALALANNRD LRVATLDIDE ARALYRIQRA AQFPAIDASV GLTSQRMSPA LRAPGQSAAI NSYDASVGLT HFEIDLFGRV RSLSHAAQEQ YLATEEARRS VHISLVAEVA NTYLTLLADR ALLALAQDTL RSQQDAADMI HRGKQAGAMA QLDEHRADTQ VQTARVAAEQ YTRQIAQDEN ALAVLIGGPL PAGVSRAAPL DDRALLAEFP AGLPSTLLER RPDIMAAEHR LIAANANIGA ARAAFFPRIT LTGALGVASA SLAGLFSGGV AWLFVPQLTL PIFNAGSNQA NLDLATVRRD INVAGYEHTI QDAFREVADN LAARATYERE VKAQEAMIRD LAETKRLADM RFRNGVDDYF GVFDAQRQLF AAQQLLVTYK LAGLTSRVTL YKALGGGWVE SAGAAAAQPR TGQAAPMARP AATPPQTTRP APVARSQTVP PRTVQPPIAQ PATAQPQTAQ AAPVAQSQTI PLRTVQPPIA QSSTSQPQTA QAAPITQART TPLRTAQPPA ARQQAPQSAP AAQPEAAPPQ TAQPPIFQP

NT Sequence

atgcatagcc caccgtttct ttcatcgccc aagcgcgcgc tgccgatcgc gctcgcgctc agcctcgccg gttgctcgat ggagccggcc taccagcggc ccgatgcgcc gatgccgtcc gcgtatccga acggccccgc ctacgcgacg ccgggcgcgc cgcgcgcgaa cccgggcgag ccggcggcgg ccgagctcgg ctggcggaat ttcctcgccg acgcgcagtt gcagcagctc gtcgcgctcg cgctcgcgaa caaccgcgac ctgcgcgtcg cgacgctcga catcgacgaa gcgcgcgcgc tgtaccgcat ccagcgcgcc gcgcagtttc ccgcaatcga tgcgagcgtc ggactcacga gccagcgcat gagccccgcg ctgcgcgcgc cggggcagtc cgcggcgatc aacagctatg acgcgagcgt cggcctcacc catttcgaga tcgacctgtt cggccgcgtg cgcagcctga gccacgccgc gcaggagcaa tacctcgcga ccgaggaggc gcggcgcagc gtccacatca gcctcgtcgc cgaagtcgcg aacacctacc tcacgctgct cgccgatcgc gcgctgctcg cgctcgcgca ggacacgctc aggagccagc aggacgccgc cgacatgatc caccgcggca agcaggcggg cgcgatggcg cagctcgacg agcatcgcgc ggacacgcag gtgcagaccg cacgcgtcgc cgccgagcaa tacacgcggc agatcgcgca ggacgagaac gcgcttgccg tgctgatcgg cgggccgctg ccggccggcg tgtcgcgcgc ggcgccgctc gacgatcgcg cgctgctcgc ggagttcccg gcgggcctgc cgtcgacgct gctcgagcgc cgccccgaca tcatggccgc cgagcatcgg ctcatcgccg cgaacgcgaa tatcggcgcg gcgcgcgcgg cgttcttccc gaggatcacg ttgacgggcg cgctcggcgt ggcgagcgcg agcctcgcgg ggctgttctc cggcggcgtc gcgtggctgt tcgtgccgca gctcacgctg ccgatcttca acgcgggcag caatcaggcg aatctcgatc tcgcgacggt gcgccgcgac atcaacgtgg ccggctacga gcacacgatc caggacgcgt tccgcgaggt cgccgacaac ctggccgcac gcgcgaccta cgagcgggaa gtgaaagcgc aggaggcgat gatccgcgac ctggccgaga cgaagcggct ggccgacatg cgttttcgca acggcgtcga cgactacttc ggcgtgttcg atgcgcaacg ccagctgttc gccgcgcagc aactgctcgt cacctacaag cttgccgggc tgacgagccg cgtgacgcta tacaaggcgc tcggcggcgg ctgggtcgaa tcggcgggcg cggcggccgc gcaaccgcgg acggggcagg ccgcgccgat ggctcgaccg gcagccactc caccccagac gacgcgaccc gcgccggttg ctcgatcgca gacggttcca ccgcgaacgg ttcagccccc gatcgcccaa ccggcaaccg cgcaaccgca aacggcgcaa gccgcgccgg ttgctcagtc gcagacgatt ccactgcgaa cggtccagcc gcctatcgcc caatcgtcaa cctctcaacc gcaaacggcg caagccgcgc cgatcactca agcgcggacg acaccactcc gaacggctca gccgcccgcc gctcgacaac aggcgccgca atccgctccg gcggctcaac cggaggcagc tccaccgcaa acggcccaac cgccgatttt tcagccgtaa

Details for BupsE.01673.a.B2.GE42222

HARVESTED ON:	2/23/2018
SEQUENCED ON:	3/1/2018
EXPECTED MW:	64kDa
OBSERVED MW:	62kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHEP AYQRPDAPMP SAYPNGPAYA TPGAPRANPG EPAAAELGWR NFLADAQLQQ LVALALANNR DLRVATLDID EARALYRIQR AAQFPAIDAS VGLTSQRMSP ALRAPGQSAA INSYDASVGL THFEIDLFGR VRSLSHAAQE QYLATEEARR SVHISLVAEV ANTYLTLLAD RALLALAQDT LRSQQDAADM IHRGKQAGAM AQLDEHRADT QVQTARVAAE QYTRQIAQDE NALAVLIGGP LPAGVSRAAP LDDRALLAEF PAGLPSTLLE RRPDIMAAEH RLIAANANIG AARAAFFPRI TLTGALGVAS ASLAGLFSGG VAWLFVPQLT LPIFNAGSNQ ANLDLATVRR DINVAGYEHT IQDAFREVAD NLAARATYER EVKAQEAMIR DLAETKRLAD MRFRNGVDDY FGVFDAQRQL FAAQQLLVTY KLAGLTSRVT LYKALGGGWV ESAGAAAAQP RTGQAAPMAR PAATPPQTTR PAPVARSQTV PPRTVQPPIA QPATAQPQTA QAAPVAQSQT IPLRTVQPPI AQSSTSQPQT AQAAPITQAR TTPLRTAQPP AARQQAPQSA PAAQPEAAPP QTAQPPIFQP

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgagccgg cctaccagcg gcccgatgcg ccgatgccgt ccgcgtatcc gaacggcccc gcctacgcga cgccgggcgc gccgcgcgcg aacccgggcg agccggcggc ggccgagctc ggctggcgga atttcctcgc cgacgcgcag ttgcagcagc tcgtcgcgct cgcgctcgcg aacaaccgcg acctgcgcgt cgcgacgctc gacatcgacg aagcgcgcgc gctgtaccgc atccagcgcg ccgcgcagtt tcccgcaatc gatgcgagcg tcggactcac gagccagcgc atgagccccg cgctgcgcgc gccggggcag tccgcggcga tcaacagcta tgacgcgagc gtcggcctca cccatttcga gatcgacctg ttcggccgcg tgcgcagcct gagccacgcc gcgcaggagc aatacctcgc gaccgaggag gcgcggcgca gcgtccacat cagcctcgtc gccgaagtcg cgaacaccta cctcacgctg ctcgccgatc gcgcgctgct cgcgctcgcg caggacacgc tcaggagcca gcaggacgcc gccgacatga tccaccgcgg caagcaggcg ggcgcgatgg cgcagctcga cgagcatcgc gcggacacgc aggtgcagac cgcacgcgtc gccgccgagc aatacacgcg gcagatcgcg caggacgaga acgcgcttgc cgtgctgatc ggcgggccgc tgccggccgg cgtgtcgcgc gcggcgccgc tcgacgatcg cgcgctgctc gcggagttcc cggcgggcct gccgtcgacg ctgctcgagc gccgccccga catcatggcc gccgagcatc ggctcatcgc cgcgaacgcg aatatcggcg cggcgcgcgc ggcgttcttc ccgaggatca cgttgacggg cgcgctcggc gtggcgagcg cgagcctcgc ggggctgttc tccggcggcg tcgcgtggct gttcgtgccg cagctcacgc tgccgatctt caacgcgggc agcaatcagg cgaatctcga tctcgcgacg gtgcgccgcg acatcaacgt ggccggctac gagcacacga tccaggacgc gttccgcgag gtcgccgaca acctggccgc acgcgcgacc tacgagcggg aagtgaaagc gcaggaggcg atgatccgcg acctggccga gacgaagcgg ctggccgaca tgcgttttcg caacggcgtc gacgactact tcggcgtgtt cgatgcgcaa cgccagctgt tcgccgcgca gcaactgctc gtcacctaca agcttgccgg gctgacgagc cgcgtgacgc tatacaaggc gctcggcggc ggctgggtcg aatcggcggg cgcggcggcc gcgcaaccgc ggacggggca ggccgcgccg atggctcgac cggcagccac tccaccccag acgacgcgac ccgcgccggt tgctcgatcg cagacggttc caccgcgaac ggttcagccc ccgatcgccc aaccggcaac cgcgcaaccg caaacggcgc aagccgcgcc ggttgctcag tcgcagacga ttccactgcg aacggtccag ccgcctatcg cccaatcgtc aacctctcaa ccgcaaacgg cgcaagccgc gccgatcact caagcgcgga cgacaccact ccgaacggct cagccgcccg ccgctcgaca acaggcgccg caatccgctc cggcggctca accggaggca gctccaccgc aaacggccca accgccgatt tttcagccgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for BupsE.01673.a.B2.PW38474

PURIFICATION DATe:	5/14/2018
CONCENTRATION:	14.11mg/ml
OBSERVED MW:	68kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGAGCCGG CCTACCAGCG GCCCGATGCG CCGATGCCGT CCGCGTATCC GAACGGCCCC GCCTACGCGA CGCCGGGCGC GCCGCGCGCG AACCCGGGCG AGCCGGCGGC GGCCGAGCTC GGCTGGCGGA ATTTCCTCGC CGACGCGCAG TTGCAGCAGC TCGTCGCGCT CGCGCTCGCG AACAACCGCG ACCTGCGCGT CGCGACGCTC GACATCGACG AAGCGCGCGC GCTGTACCGC ATCCAGCGCG CCGCGCAGTT TCCCGCAATC GATGCGAGCG TCGGACTCAC GAGCCAGCGC ATGAGCCCCG CGCTGCGCGC GCCGGGGCAG TCCGCGGCGA TCAACAGCTA TGACGCGAGC GTCGGCCTCA CCCATTTCGA GATCGACCTG TTCGGCCGCG TGCGCAGCCT GAGCCACGCC GCGCAGGAGC AATACCTCGC GACCGAGGAG GCGCGGCGCA GCGTCCACAT CAGCCTCGTC GCCGAAGTCG CGAACACCTA CCTCACGCTG CTCGCCGATC GCGCGCTGCT CGCGCTCGCG CAGGACACGC TCAGGAGCCA GCAGGACGCC GCCGACATGA TCCACCGCGG CAAGCAGGCG GGCGCGATGG CGCAGCTCGA CGAGCATCGC GCGGACACGC AGGTGCAGAC CGCACGCGTC GCCGCCGAGC AATACACGCG GCAGATCGCG CAGGACGAGA ACGCGCTTGC CGTGCTGATC GGCGGGCCGC TGCCGGCCGG CGTGTCGCGC GCGGCGCCGC TCGACGATCG CGCGCTGCTC GCGGAGTTCC CGGCGGGCCT GCCGTCGACG CTGCTCGAGC GCCGCCCCGA CATCATGGCC GCCGAGCATC GGCTCATCGC CGCGAACGCG AATATCGGCG CGGCGCGCGC GGCGTTCTTC CCGAGGATCA CGTTGACGGG CGCGCTCGGC GTGGCGAGCG CGAGCCTCGC GGGGCTGTTC TCCGGCGGCG TCGCGTGGCT GTTCGTGCCG CAGCTCACGC TGCCGATCTT CAACGCGGGC AGCAATCAGG CGAATCTCGA TCTCGCGACG GTGCGCCGCG ACATCAACGT GGCCGGCTAC GAGCACACGA TCCAGGACGC GTTCCGCGAG GTCGCCGACA ACCTGGCCGC ACGCGCGACC TACGAGCGGG AAGTGAAAGC GCAGGAGGCG ATGATCCGCG ACCTGGCCGA GACGAAGCGG CTGGCCGACA TGCGTTTTCG CAACGGCGTC GACGACTACT TCGGCGTGTT CGATGCGCAA CGCCAGCTGT TCGCCGCGCA GCAACTGCTC GTCACCTACA AGCTTGCCGG GCTGACGAGC CGCGTGACGC TATACAAGGC GCTCGGCGGC GGCTGGGTCG AATCGGCGGG CGCGGCGGCC GCGCAACCGC GGACGGGGCA GGCCGCGCCG ATGGCTCGAC CGGCAGCCAC TCCACCCCAG ACGACGCGAC CCGCGCCGGT TGCTCGATCG CAGACGGTTC CACCGCGAAC GGTTCAGCCC CCGATCGCCC AACCGGCAAC CGCGCAACCG CAAACGGCGC AAGCCGCGCC GGTTGCTCAG TCGCAGACGA TTCCACTGCG AACGGTCCAG CCGCCTATCG CCCAATCGTC AACCTCTCAA CCGCAAACGG CGCAAGCCGC GCCGATCACT CAAGCGCGGA CGACACCACT CCGAACGGCT CAGCCGCCCG CCGCTCGACA ACAGGCGCCG CAATCCGCTC CGGCGGCTCA ACCGGAGGCA GCTCCACCGC AAACGGCCCA ACCGCCGATT TTTCAGCCGT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT