SSGCID: Target Page

BupsE.01668.a

Putative sensor histidine kinase (BPSS0813)

CENTER ID:	BupsE.01668.a
ORGANISM:	Burkholderia pseudomallei K96243
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsE.01668.a.B1.GE42221	full length		1	471

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272560.51.peg.7082
UniProt:	Q63M47

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSESTVTQMS AAIVEQLNCG VFSVDHDMRI LAWNGFMQYH TGRSRDDAIG QDLFALFPEL PARWLRKKLE SVFVLGVAMY TSWEHRPYLF RFDHSRPITG TIDAMRQNCS FVPLDDGRGG VAAVGVTIVD ATDICIAHEV LGNREKRLTD ALAELTARHA ELSDLNRQLA HAHQQLLQSE KLAAIGQLAA GIAHEINNPV GFVLSNVNTL GGYLGALIAH AHAVERLVAE RQPQLAPALA ELARGADLDY LCEDAPALVD ESKEGLARVR KIVVDLRDFS RVDSVHQWEW VDIHHCIEST LNIVRNEVKY AADLVREYAS LPMVRCIPSQ VNQVVLNLVV NAAQSYSSMR ADGGHGDVPR GTITIRTGVD AGDCPRIWFE VIDAGCGIAP ENLKRIFDPF FTTKPVGKGT GLGLSVAYGI VSAHGGEIAV SSAFGAGTTF RVTLPVERTG EPQPEAAAVS HSTPVASGIA S

NT Sequence

atgtccgaat ccaccgttac gcagatgtcc gcagcaatcg tcgagcagct caactgcggc gtgttctcgg tcgaccacga catgcgcatc ctcgcgtgga acggcttcat gcagtaccac acgggccgct cgcgcgacga cgcgatcggc caggatctgt tcgcgctctt tccggagctg cccgcgcgct ggctgcgcaa gaagctggag agcgtgttcg tgctcggcgt cgcgatgtac acgtcgtggg agcaccgtcc ctatttgttc cgcttcgatc attcgcggcc gatcaccggc acgatcgacg cgatgcggca gaactgcagc ttcgtgccgc tcgacgatgg gcgcggcggc gtcgccgcgg tgggcgtgac gatcgtcgac gccaccgata tctgcatcgc gcacgaagtg ctgggcaacc gcgagaagcg gctgaccgac gcgctcgccg agctgaccgc gcgccatgcc gagctgtcgg acctgaaccg ccagctcgca catgcgcatc agcaactgct gcagtcggag aagctcgcgg cgatcggcca gctcgccgcc ggcatcgcgc acgaaatcaa caatccggtc ggcttcgtgc tgtcgaacgt gaacacgctc ggcggctatc tcggcgcgct gatcgcgcat gcgcacgcgg tcgagcggct cgtcgccgag cgccagccgc agctcgcgcc ggcgctcgcc gagctcgcgc ggggcgcgga tctcgactat ctgtgcgagg acgcgccggc gctcgtcgac gaatcgaagg aagggctcgc gcgcgtgcgc aagatcgtcg tcgatctgcg cgatttctcg cgcgtcgaca gcgtgcacca gtgggaatgg gtcgacatcc atcactgcat cgaatcgacg ctgaacatcg tgcgcaacga agtgaaatac gcggccgatc tcgtgcgcga gtacgcatcg ctgccgatgg tccgctgcat cccgtcgcag gtcaaccagg tggtgctgaa cctcgtcgtc aacgccgcgc aatcgtattc gagcatgcgc gcggacggcg gccacggcga cgtgccgcgc ggcacgatca cgatccgcac gggcgtcgac gcgggcgact gcccgcgcat ctggttcgag gtcatcgatg cgggctgcgg gatcgccccc gagaatctca agcggatctt cgatccgttc ttcacgacga agccggtcgg caagggcacg gggctcgggc tgtcggtcgc ctacggcatc gtcagcgcgc acggcggcga gatcgcggtg tcgagcgcgt tcggcgcggg cacgacgttt cgcgtgacgc tgcccgtcga gcgcaccggc gagccgcagc ccgaagcggc ggccgtttcc cattcgacgc cggttgcgtc cggcatcgca agctga

Details for BupsE.01668.a.B1.GE42221

HARVESTED ON:	2/23/2018
SEQUENCED ON:	3/1/2018
EXPECTED MW:	52kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMS ESTVTQMSAA IVEQLNCGVF SVDHDMRILA WNGFMQYHTG RSRDDAIGQD LFALFPELPA RWLRKKLESV FVLGVAMYTS WEHRPYLFRF DHSRPITGTI DAMRQNCSFV PLDDGRGGVA AVGVTIVDAT DICIAHEVLG NREKRLTDAL AELTARHAEL SDLNRQLAHA HQQLLQSEKL AAIGQLAAGI AHEINNPVGF VLSNVNTLGG YLGALIAHAH AVERLVAERQ PQLAPALAEL ARGADLDYLC EDAPALVDES KEGLARVRKI VVDLRDFSRV DSVHQWEWVD IHHCIESTLN IVRNEVKYAA DLVREYASLP MVRCIPSQVN QVVLNLVVNA AQSYSSMRAD GGHGDVPRGT ITIRTGVDAG DCPRIWFEVI DAGCGIAPEN LKRIFDPFFT TKPVGKGTGL GLSVAYGIVS AHGGEIAVSS AFGAGTTFRV TLPVERTGEP QPEAAAVSHS T

Validated NT Sequence

cggcgtcgaa tgggaaacgg ccgccgcttc gggctgcggc tcgccggtgc gctcgacggg cagcgtcacg cgaaacgtcg tgcccgcgcc gaacgcgctc gacaccgcga tctcgccgcc gtgcgcgctg acgatgccgt aggcgaccga cagcccgagc cccgtgccct tgccgaccgg cttcgtcgtg aagaacggat cgaagatccg cttgagattc tcgggggcga tcccgcagcc cgcatcgatg acctcgaacc agatgcgcgg gcagtcgccc gcgtcgacgc ccgtgcggat cgtgatcgtg ccgcgcggca cgtcgccgtg gccgccgtcc gcgcgcatgc tcgaatacga ttgcgcggcg ttgacgacga ggttcagcac cacctggttg acctgcgacg ggatgcagcg gaccatcggc agcgatgcgt actcgcgcac gagatcggcc gcgtatttca cttcgttgcg cacgatgttc agcgtcgatt cgatgcagtg atggatgtcg acccattccc actggtgcac gctgtcgacg cgcgagaaat cgcgcagatc gacgacgatc ttgcgcacgc gcgcgagccc ttccttcgat tcgtcgacga gcgccggcgc gtcctcgcac agatagtcga gatccgcgcc ccgcgcgagc tcggcgagcg ccggcgcgag ctgcggctgg cgctcggcga cgagccgctc gaccgcgtgc gcatgcgcga tcagcgcgcc gagatagccg ccgagcgtgt tcacgttcga cagcacgaag ccgaccggat tgttgatttc gtgcgcgatg ccggcggcga gctggccgat cgccgcgagc ttctccgact gcagcagttg ctgatgcgca tgtgcgagct ggcggttcag gtccgacagc tcggcatggc gcgcggtcag ctcggcgagc gcgtcggtca gccgcttctc gcggttgccc agcacttcgt gcgcgatgca gatatcggtg gcgtcgacga tcgtcacgcc caccgcggcg acgccgccgc gcccatcgtc gagcggcacg aagctgcagt tctgccgcat cgcgtcgatc gtgccggtga tcggccgcga atgatcgaag cggaacaaat agggacggtg ctcccacgac gtgtacatcg cgacgccgag cacgaacacg ctctccagct tcttgcgcag ccagcgcgcg ggcagctccg gaaagagcgc gaacagatcc tggccgatcg cgtcgtcgcg cgagcggccc gtgtggtact gcatgaagcc gttccacgcg aggatgcgca tgtcgtggtc gaccgagaac acgccgcagt tgagctgctc gacgattgct gcggacatct gcgtaacggt ggattcggac atatggtggt ggtggtggtg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg aatccaccgt tacgcagatg tccgcagcaa tcgtcgagca gctcaactgc ggcgtgttct cggtcgacca cgacatgcgc atcctcgcgt ggaacggctt catgcagtac cacacgggcc gctcgcgcga cgacgcgatc ggccaggatc tgttcgcgct ctttccggag ctgcccgcgc gctggctgcg caagaagctg gagagcgtgt tcgtgctcgg cgtcgcgatg tacacgtcgt gggagcaccg tccctatttg ttccgcttcg atcattcgcg gccgatcacc ggcacgatcg acgcgatgcg gcagaactgc agcttcgtgc cgctcgacga tgggcgcggc ggcgtcgccg cggtgggcgt gacgatcgtc gacgccaccg atatctgcat cgcgcacgaa gtgctgggca accgcgagaa gcggctgacc gacgcgctcg ccgagctgac cgcgcgccat gccgagctgt cggacctgaa ccgccagctc gcacatgcgc atcagcaact gctgcagtcg gagaagctcg cggcgatcgg ccagctcgcc gccggcatcg cgcacgaaat caacaatccg gtcggcttcg tgctgtcgaa cgtgaacacg ctcggcggct atctcggcgc gctgatcgcg catgcgcacg cggtcgagcg gctcgtcgcc gagcgccagc cgcagctcgc gccggcgctc gccgagctcg cgcggggcgc ggatctcgac tatctgtgcg aggacgcgcc ggcgctcgtc gacgaatcga aggaagggct cgcgcgcgtg cgcaagatcg tcgtcgatct gcgcgatttc tcgcgcgtcg acagcgtgca ccagtgggaa tgggtcgaca tccatcactg catcgaatcg acgctgaaca tcgtgcgcaa cgaagtgaaa tacgcggccg atctcgtgcg cgagtacgca tcgctgccga tggtccgctg catcccgtcg caggtcaacc aggtggtgct gaacctcgtc gtcaacgccg cgcaatcgta ttcgagcatg cgcgcggacg gcggccacgg cgacgtgccg cgcggcacga tcacgatccg cacgggcgtc gacgcgggcg actgcccgcg catctggttc gaggtcatcg atgcgggctg cgggatcgcc cccgagaatc tcaagcggat cttcgatccg ttcttcacga cgaagccggt cggcaagggc acggggctcg ggctgtcggt cgcctacggc atcgtcagcg cgcacggcgg cgagatcgcg gtgtcgagcg cgttcggcgc gggcacgacg tttcgcgtga cgctgcccgt cgagcgcacc ggcgagccgc agcccgaagc ggcggccgtt tcccattcga cgccggttgc gtccggcatc gcaagctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t