BupsE.01646.a
Putative cystathionine gamma-synthase
| CENTER ID: | BupsE.01646.a |
| ORGANISM: | Burkholderia pseudomallei K96243 |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsE.01646.a.A1.GE34328 | full length | 1 | 433 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
7SWH
DEPOSITED: 11/19/2021
DETERMINATION: XRay
CLONE: BupsE.01646.a.A1.GE34328
PROTEIN: BupsE.01646.a.A1.PW34938
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126775 |
| PATRIC ID: | fig|272560.6.peg.5014 |
| RefSeq: | YP_110922.1 |
| UniProt: | Q63LU9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTYSSYHKKD IEGRPLHPET QMMSYGFDPF LSEGAVKPPV FLTSTFAFRS AEDGADFFDI VSGRKPLPQG EAAGLVYSRF NHPNLEIVED RLALLDGSEA AVVTSSGMSA ISAIFLAFLR PGDQLVQSVP LYGGTETLIA KYFREWGVGA HSIDNGLSPQ AIGAALEAAA QQGPVRLCYV ETPANPTNAL IDLDGMRREL DAFEARHGYR PISVCDNTLL GPIFQKPSEH GVDMSVYSLT KYVGGHSDLV GGGVTGRRDL VAKVRAVRSA FGSQLDPHSS WMLIRSMETV VLRMKQAART ASAVAKWLAT NPHQKVDVYH PELIVDDAYQ AVYKRQCTGA GSTFAFVLNG GRAEAFRFIN ALHLFKSAVS LGGTESLICH PASTTHSGVP EAARKAAGVS EGLIRVSIGL EHEEDLIADL DHAFRRSGLA TGV
NT Sequence
atgacgtatt cgagctatca caagaaggac atcgagggcc gtccgctgca cccggaaacg cagatgatgt cgtacggatt cgatccgttc ttgtccgaag gcgcggtgaa gcctccggta ttcctcacgt cgacgttcgc gttccgctcg gcggaggacg gcgccgattt cttcgatatc gtctcgggcc gcaagccgtt gccgcagggc gaggcggcgg gcctcgtcta cagccgcttc aatcatccga atctcgagat cgtcgaggat cgcctcgcgc tgctcgacgg ctccgaggcg gccgtcgtca cgtcgagcgg gatgtcggcg atcagcgcga tctttctcgc gttcctgcgg ccgggcgatc agctcgtgca atcggtgccg ctgtacggcg gcacggaaac gctgatcgcg aaatacttcc gcgaatgggg tgtcggcgcg cattcgatcg acaacggcct gtcgccgcaa gcgatcggcg cggcgcttga agccgccgcg cagcaggggc ccgtgcggct ctgctacgtc gagacgccgg cgaatccgac gaacgcgctg atcgacctcg acggcatgcg ccgcgagctc gacgcgttcg aggcgcgaca cggctatcgt ccgatttcgg tgtgcgacaa cacgctgctc gggccgatct tccagaagcc gagcgagcac ggcgtcgaca tgtcggtgta ctcgctgacc aagtacgtcg gcggccacag cgatctcgtc gggggcggcg tgacggggcg gcgggatctc gtcgcgaagg tccgcgcggt gcgcagcgca ttcggctcgc agctcgatcc gcattcgtcg tggatgctga tccgttcgat ggaaacggtc gtgctgcgga tgaagcaggc ggcgcgcacc gcgtcggcgg tcgcgaagtg gctcgcgacg aatccgcatc agaaggtcga cgtgtaccac ccggagctga tcgtcgacga tgcgtatcag gccgtgtaca agcggcaatg cacgggcgct ggctcgacgt tcgcgttcgt gctgaacggc ggccgcgcgg aagcattccg cttcatcaac gcgttgcatc tgttcaagtc cgcggtgagt ctcggcggca ccgaatcgtt gatctgccat ccggcgtcga cgacgcactc cggcgtgccg gaggcggcgc gcaaggcggc gggcgtgtcg gaagggttga ttcgcgtgtc gatcgggctc gagcacgagg aggacctgat cgccgatctc gatcacgcgt ttcgccggtc cggcctcgcg acgggcgtct ga
Details for BupsE.01646.a.A1.GE34328
| HARVESTED ON: | 1/10/2012 |
| SEQUENCED ON: | 1/13/2012 |
| EXPECTED MW: | 48kDa |
| OBSERVED MW: | 48kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 64 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MGTLEAQTQG PGSMTYSSYH KKDIEGRPLH PETQMMSYGF DPFLSEGAVK PPVFLTSTFA FRSAEDGADF FDIVSGRKPL PQGEAAGLVY SRFNHPNLEI VEDRLALLDG SEAAVVTSSG MSAISAIFLA FLRPGDQLVQ SVPLYGGTET LIAKYFREWG VGAHSIDNGL SPQAIGAALE AAAQQGPVRL CYVETPANPT NALIDLDGMR RELDAFEARH GYRPISVCDN TLLXADLPEA ERARRRHVGV LADQVRRRPQ RSRRGRRDGA AGSRREGPRG AQRIRLAARS AFVVDADPFD GNGRAADEAG GAHRVGGREV ARDESASEGR RVPPGADRRR CVSGRVQAAM HGRWLDVRVR AERRPRGSIP LHQRVASVQV RGESRRHRIV DLPSGVDDAL RRAGGGAQGG GRVGRVDSRV DRARARGGPD RRSRSRVSPV RPRDGR
Validated NT Sequence
agacgcccgt cgcgaggccg gaccggcgaa acgcgtgatc gagatcggcg atcaggtcct cctcgtgctc gagcccgatc gacacgcgaa tcaacccttc cgacacgccc gccgccttgc gcgccgcctc cggcacgccg gagtgcgtcg tcgacgccgg atggcagatc aacgattcgg tgccgccgag actcaccgcg gacttgaaca gatgcaacgc gttgatgaag cggaatgctt ccgcgcggcc gccgttcagc acgaacgcga acgtcgagcc agcgcccgtg cattgccgct tgtacacggc ctgatacgca tcgtcgacga tcagctccgg gtggtacacg tcgaccttct gatgcggatt cgtcgcgagc cacttcgcga ccgccgacgc ggtgcgcgcc gcctgcttca tccgcagcac gaccgtttcc atcgaacgga tcagcatcca cgacgaangc ggatcgagct gcgagccgaa tgcgctgcgc accgcgcgga ccttcgcgac gagatcccgc cgccccgtca cgccgccccc gacgagatcg ctgtggccgc cgacgtactt ggtcagcgag tacaccgaca tgtcgacgcc gtgctcgctc ggcttctgga agatcggccc ngagcagcgt gttgtcgcac accgaaatcg gacgatagcc gtgtcgcgcc tcgaacgcgt cgagctcgcg gcgcatgccg tcgaggtcga tcagcgcgtt cgtcggattc gccggcgtct cgacgtagca gagccgcacg ggcccctgct gcgcggcggc ttcaagcgcc gcgccgatcg cttgcggcga caggccgttg tcgatcgaat gcgcgccgac accccattcg cggaagtatt tcgcgatcag cgtttccgtg ccgccgtaca gcggcaccga ttgcacgagc tgatcgcccg gccgcaggaa cgcgagaaag atcgcgctga tcgccgacat cccgctcgac gtgacgacgg ccgcctcgga gccgtcgagc agcgcgaggc gatcctcgac gatctcgaga ttcggatgat tgaagcggct gtagacgagg cccgccgcct cgccctgcgg caacggcttg cggcccgaga cgatatcgaa gaaatcggcg ccgtcctccg ccgagcggaa cgcgaacgtc gacgtgagga ataccggagg cttcaccgcg ccttcggaca agaacggatc gaatccgtac gacatcatct gcgtttccgg gtgcagcgga cggccctcga tgtccttctt gtgatagctc gaatacgtca tcgaaccagg accctgggtc tgagcttcca gggtacccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTYSSYHKK DIEGRPLHPE TQMMSYGFDP FLSEGAVKPP VFLTSTFAFR SAEDGADFFD IVSGRKPLPQ GEAAGLVYSR FNHPNLEIVE DRLALLDGSE AAVVTSSGMS AISAIFLAFL RPGDQLVQSV PLYGGTETLI AKYFREWGVG AHSIDNGLSP QAIGAALEAA AQQGPVRLCY VETPANPTNA LIDLDGMRRE LDAFEARHGY RPISVCDNTL LGPIFQKPSE HGVDMSVYSL TKYVGGHSDL VGGGVTGRRD LVAKVRAVRS AFGSQLDPHS SWMLIRSMET VVLRMKQAAR TASAVAKWLA TNPHQKVDVY HPELIVDDAY QAVYKRQCTG AGSTFAFVLN GGRAEAFRFI NALHLFKSAV SLGGTESLIC HPASTTHSGV PEAARKAAGV SEGLIRVSIG LEHEEDLIAD LDHAFRRSGL ATGV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgtattcg agctatcaca agaaggacat cgagggccgt ccgctgcacc cggaaacgca gatgatgtcg tacggattcg atccgttctt gtccgaaggc gcggtgaagc ctccggtatt cctcacgtcg acgttcgcgt tccgctcggc ggaggacggc gccgatttct tcgatatcgt ctcgggccgc aagccgttgc cgcagggcga ggcggcgggc ctcgtctaca gccgcttcaa tcatccgaat ctcgagatcg tcgaggatcg cctcgcgctg ctcgacggct ccgaggcggc cgtcgtcacg tcgagcggga tgtcggcgat cagcgcgatc tttctcgcgt tcctgcggcc gggcgatcag ctcgtgcaat cggtgccgct gtacggcggc acggaaacgc tgatcgcgaa atacttccgc gaatggggtg tcggcgcgca ttcgatcgac aacggcctgt cgccgcaagc gatcggcgcg gcgcttgaag ccgccgcgca gcaggggccc gtgcggctct gctacgtcga gacgccggcg aatccgacga acgcgctgat cgacctcgac ggcatgcgcc gcgagctcga cgcgttcgag gcgcgacacg gctatcgtcc gatttcggtg tgcgacaaca cgctgctcgg gccgatcttc cagaagccga gcgagcacgg cgtcgacatg tcggtgtact cgctgaccaa gtacgtcggc ggccacagcg atctcgtcgg gggcggcgtg acggggcggc gggatctcgt cgcgaaggtc cgcgcggtgc gcagcgcatt cggctcgcag ctcgatccgc attcgtcgtg gatgctgatc cgttcgatgg aaacggtcgt gctgcggatg aagcaggcgg cgcgcaccgc gtcggcggtc gcgaagtggc tcgcgacgaa tccgcatcag aaggtcgacg tgtaccaccc ggagctgatc gtcgacgatg cgtatcaggc cgtgtacaag cggcaatgca cgggcgctgg ctcgacgttc gcgttcgtgc tgaacggcgg ccgcgcggaa gcattccgct tcatcaacgc gttgcatctg ttcaagtccg cggtgagtct cggcggcacc gaatcgttga tctgccatcc ggcgtcgacg acgcactccg gcgtgccgga ggcggcgcgc aaggcggcgg gcgtgtcgga agggttgatt cgcgtgtcga tcgggctcga gcacgaggag gacctgatcg ccgatctcga tcacgcgttt cgccggtccg gcctcgcgac gggcgtctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa