BupsE.01471.a
Aromatic-amino-acid aminotransferase (EC 2.6.1.57)
| CENTER ID: | BupsE.01471.a |
| ORGANISM: | Burkholderia pseudomallei K96243 |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsE.01471.a.A1.GE34338 | full length | 1 | 399 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsE.01471.a.A1.PW34948 | full length | 1 | 399 |
Structures
4EFF
DEPOSITED: 3/29/2012
DETERMINATION: XRay
CLONE: BupsE.01471.a.A1.GE34338
PROTEIN: BupsE.01471.a.A1.PW34948
4F4E
DEPOSITED: 5/10/2012
DETERMINATION: XRay
CLONE: BupsE.01471.a.A1.GE34338
PROTEIN: BupsE.01471.a.A1.PW34948
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126737 |
| PATRIC ID: | fig|272560.6.peg.4349 |
| RefSeq: | YP_110375.1 |
| UniProt: | Q63NE4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLFSAVELA PRDPILGLNE AFNADTRPTK VNLGVGVYTN EDGKIPLLRA VRDAEKARVE AGLPRGYLPI DGIAAYDASV QKLLLGDDSP LIAAGRVVTA QALGGTGALK IGADFLRTLN PKAKVAISDP SWENHRALFD MAGFEVVAYP YYDAKTNGVN FDGMLAALNG YEPGTIVVLH ACCHNPTGVD LNDAQWAQVV EVVKARRLVP FLDIAYQGFG ESIEADAAAV RLFAAANLNV FVSSSFSKSF SLYGERVGAL SIITDSKDEA ARVLSQLKRV IRTNYSNPPT HGGAIVAAVL ASPELRASWV QELGEMRDRI RAMRNGLVER LKAAGIERDF SFINAQRGMF SYSGLTSAQV DRLREEFGIY AVSTGRICVA ALNTRNLDVV ANAIAAVLK
NT Sequence
atgtcgctct tctccgctgt cgaacttgcc ccccgcgacc ctatcctggg cctgaacgaa gccttcaacg ccgacacgcg cccgaccaag gtgaacctgg gcgtcggcgt ctacaccaac gaggacggca agattccgct gctgcgcgcc gtgcgcgacg cggaaaaggc acgggtcgaa gccggcctgc cgcgcggcta cctgccgatc gacgggatcg ccgcctatga cgcatcggtg cagaagctgc tgctcggcga cgattcgccg ctgatcgcgg cgggccgcgt cgtgacggcg caggcgctcg gcggcacggg cgcgctgaag atcggcgcgg atttcctgcg caccctgaac ccgaaggcga aggtggcgat cagcgatccg agctgggaaa accaccgtgc cctgttcgac atggcgggct tcgaagtcgt cgcgtacccg tactacgacg cgaagacgaa cggcgtgaac ttcgacggca tgctggccgc gctcaacggc tacgagccgg gcacgatcgt cgtgctgcac gcgtgctgcc acaacccgac gggcgtggac ctgaacgatg cgcaatgggc gcaggtggtc gaggtcgtga aggcgcgtcg gctcgtgccg ttcctggaca tcgcctatca aggcttcggc gaaagcatcg aggccgacgc ggccgcggtg cgcctgttcg cggcggccaa tctgaacgtg ttcgtatcgt cgtcgttctc gaagtcgttc tcgctgtatg gcgagcgcgt cggcgcgctg tcgatcatca ccgacagcaa ggacgaagct gcacgcgtgc tgtcgcaact gaagcgcgtg attcgcacga actattcgaa cccgccgacg cacggcggcg cgatcgttgc cgcggtgctc gcctcgccgg aactgcgcgc ttcgtgggtg caggaactgg gcgagatgcg cgatcgcatc cgcgcaatgc gcaacggcct cgtcgagcgc ctgaaggcgg ccggcatcga gcgcgacttc agcttcatca acgcgcaacg cgggatgttc tcgtactcgg gcctgacgtc cgcgcaagtc gaccgtctgc gcgaagaatt cggcatctat gcggtcagca ccggccggat ctgcgtcgcg gcgctcaaca cgcgcaacct cgacgtcgtc gcgaacgcga tcgccgcggt gttgaagtaa
Details for BupsE.01471.a.A1.GE34338
| HARVESTED ON: | 1/10/2012 |
| SEQUENCED ON: | 1/25/2012 |
| EXPECTED MW: | 45kDa |
| OBSERVED MW: | 45kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 94 |
Validated AA Sequence
MSLFSAVELA PRDPILGLNE AFNADTRPTK VNLGVGVYTN EDGKIPLLRA VRDAEKARVE AGLPRGYLPI DGIAAYDASV QKLLLGDDSP LIAAGRVVTA QALGGTGALK IGADFLRTLN PKAKVAISDP SWENHRALFD MAGFEVVAYP YYDAKTNGVN FDGMLAALNG YEPGTIVVLH ACCHNPTGVD LNDAQWAQVV EVVKARRLVP FLDIAYQGFG ESIEADAAAV RLFAAANLNV FVSSSFSKSF SLYGERVGAL SIITDSKDEA ARVLSQLKRV IRTNYSNPPT HGGAIVAAVL ASPELRASWV QELGEMRDRI RAMRNGLVER LKAAGIERDF SFINAQRGMF SYSGLTSAQV DRLREEFGIY AVSTGRICVA ALNTRNLDVV ANAIA
Validated NT Sequence
gcggcgatcg cgttcgcgac gacgtcgagg ttgcgcgtgt tgagcgccgc gacgcagatc cggccggtgc tgaccgcata gatgccgaat tcttcgcgca gacggtcgac ttgcgcggac gtcaggcccg agtacgagaa catcccgcgt tgcgcgttga tgaagctgaa gtcgcgctcg atgccggccg ccttcaggcg ctcgacgagg ccgttgcgca ttgcgcggat gcgatcgcgc atctcgccca gttcctgcac ccacgaagcg cgcagttccg gcgaggcgag caccgcggca acgatcgcgc cgccgtgcgt cggcgggttc gaatagttcg tgcgaatcac gcgcttcagt tgcgacagca cgcgtgcagc ttcgtccttg ctgtcggtga tgatcgacag cgcgccgacg cgctcgccat acagcgagaa cgacttcgag aacgacgacg atacgaacac gttcagattg gccgccgcga acaggcgcac cgcggccgcg tcggcctcga tgctttcgcc gaagccttga taggcgatgt ccaggaacgg cacgagccga cgcgccttca cgacctcgac cacctgcgcc cattgcgcat cgttcaggtc cacgcccgtc gggttgtggc agcacgcgtg cagcacgacg atcgtgcccg gctcgtagcc gttgagcgcg gccagcatgc cgtcgaagtt cacgccgttc gtcttcgcgt cgtagtacgg gtacgcgacg acttcgaagc ccgccatgtc gaacagggca cggtggtttt cccagctcgg atcgctgatc gccaccttcg ccttcgggtt cagggtgcgc aggaaatccg cgccgatctt cagcgcgccc gtgccgccga gcgcctgcgc cgtcacgacg cggcccgccg cgatcagcgg cgaatcgtcg ccgagcagca gcttctgcac cgatgcgtca taggcggcga tcccgtcgat cggcaggtag ccgcgcggca ggccggcttc gacccgtgcc ttttccgcgt cgcgcacggc gcgcagcagc ggaatcttgc cgtcctcgtt ggtgtagacg ccgacgccca ggttcacctt ggtcgggcgc gtgtcggcgt tgaaggcttc gttcaggccc aggatagggt cgcggggggc aagttcgaca gcggagaaga gcgacatcga accaggaccc tgggtctgag cttccag
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSLFSAVEL APRDPILGLN EAFNADTRPT KVNLGVGVYT NEDGKIPLLR AVRDAEKARV EAGLPRGYLP IDGIAAYDAS VQKLLLGDDS PLIAAGRVVT AQALGGTGAL KIGADFLRTL NPKAKVAISD PSWENHRALF DMAGFEVVAY PYYDAKTNGV NFDGMLAALN GYEPGTIVVL HACCHNPTGV DLNDAQWAQV VEVVKARRLV PFLDIAYQGF GESIEADAAA VRLFAAANLN VFVSSSFSKS FSLYGERVGA LSIITDSKDE AARVLSQLKR VIRTNYSNPP THGGAIVAAV LASPELRASW VQELGEMRDR IRAMRNGLVE RLKAAGIERD FSFINAQRGM FSYSGLTSAQ VDRLREEFGI YAVSTGRICV AALNTRNLDV VANAIAAVLK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgctcttc tccgctgtcg aacttgcccc ccgcgaccct atcctgggcc tgaacgaagc cttcaacgcc gacacgcgcc cgaccaaggt gaacctgggc gtcggcgtct acaccaacga ggacggcaag attccgctgc tgcgcgccgt gcgcgacgcg gaaaaggcac gggtcgaagc cggcctgccg cgcggctacc tgccgatcga cgggatcgcc gcctatgacg catcggtgca gaagctgctg ctcggcgacg attcgccgct gatcgcggcg ggccgcgtcg tgacggcgca ggcgctcggc ggcacgggcg cgctgaagat cggcgcggat ttcctgcgca ccctgaaccc gaaggcgaag gtggcgatca gcgatccgag ctgggaaaac caccgtgccc tgttcgacat ggcgggcttc gaagtcgtcg cgtacccgta ctacgacgcg aagacgaacg gcgtgaactt cgacggcatg ctggccgcgc tcaacggcta cgagccgggc acgatcgtcg tgctgcacgc gtgctgccac aacccgacgg gcgtggacct gaacgatgcg caatgggcgc aggtggtcga ggtcgtgaag gcgcgtcggc tcgtgccgtt cctggacatc gcctatcaag gcttcggcga aagcatcgag gccgacgcgg ccgcggtgcg cctgttcgcg gcggccaatc tgaacgtgtt cgtatcgtcg tcgttctcga agtcgttctc gctgtatggc gagcgcgtcg gcgcgctgtc gatcatcacc gacagcaagg acgaagctgc acgcgtgctg tcgcaactga agcgcgtgat tcgcacgaac tattcgaacc cgccgacgca cggcggcgcg atcgttgccg cggtgctcgc ctcgccggaa ctgcgcgctt cgtgggtgca ggaactgggc gagatgcgcg atcgcatccg cgcaatgcgc aacggcctcg tcgagcgcct gaaggcggcc ggcatcgagc gcgacttcag cttcatcaac gcgcaacgcg ggatgttctc gtactcgggc ctgacgtccg cgcaagtcga ccgtctgcgc gaagaattcg gcatctatgc ggtcagcacc ggccggatct gcgtcgcggc gctcaacacg cgcaacctcg acgtcgtcgc gaacgcgatc gccgcggtgt tgaagtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BupsE.01471.a.A1.PW34948
| PURIFICATION DATe: | 2/24/2012 |
| CONCENTRATION: | 36.12mg/ml |
| OBSERVED MW: | 43kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 19 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 94 |
Protocol Notes
notes unavailable
Validated AA Sequence
MSLFSAVELA PRDPILGLNE AFNADTRPTK VNLGVGVYTN EDGKIPLLRA VRDAEKARVE AGLPRGYLPI DGIAAYDASV QKLLLGDDSP LIAAGRVVTA QALGGTGALK IGADFLRTLN PKAKVAISDP SWENHRALFD MAGFEVVAYP YYDAKTNGVN FDGMLAALNG YEPGTIVVLH ACCHNPTGVD LNDAQWAQVV EVVKARRLVP FLDIAYQGFG ESIEADAAAV RLFAAANLNV FVSSSFSKSF SLYGERVGAL SIITDSKDEA ARVLSQLKRV IRTNYSNPPT HGGAIVAAVL ASPELRASWV QELGEMRDRI RAMRNGLVER LKAAGIERDF SFINAQRGMF SYSGLTSAQV DRLREEFGIY AVSTGRICVA ALNTRNLDVV ANAIA
Validated NT Sequence
gcggcgatcg cgttcgcgac gacgtcgagg ttgcgcgtgt tgagcgccgc gacgcagatc cggccggtgc tgaccgcata gatgccgaat tcttcgcgca gacggtcgac ttgcgcggac gtcaggcccg agtacgagaa catcccgcgt tgcgcgttga tgaagctgaa gtcgcgctcg atgccggccg ccttcaggcg ctcgacgagg ccgttgcgca ttgcgcggat gcgatcgcgc atctcgccca gttcctgcac ccacgaagcg cgcagttccg gcgaggcgag caccgcggca acgatcgcgc cgccgtgcgt cggcgggttc gaatagttcg tgcgaatcac gcgcttcagt tgcgacagca cgcgtgcagc ttcgtccttg ctgtcggtga tgatcgacag cgcgccgacg cgctcgccat acagcgagaa cgacttcgag aacgacgacg atacgaacac gttcagattg gccgccgcga acaggcgcac cgcggccgcg tcggcctcga tgctttcgcc gaagccttga taggcgatgt ccaggaacgg cacgagccga cgcgccttca cgacctcgac cacctgcgcc cattgcgcat cgttcaggtc cacgcccgtc gggttgtggc agcacgcgtg cagcacgacg atcgtgcccg gctcgtagcc gttgagcgcg gccagcatgc cgtcgaagtt cacgccgttc gtcttcgcgt cgtagtacgg gtacgcgacg acttcgaagc ccgccatgtc gaacagggca cggtggtttt cccagctcgg atcgctgatc gccaccttcg ccttcgggtt cagggtgcgc aggaaatccg cgccgatctt cagcgcgccc gtgccgccga gcgcctgcgc cgtcacgacg cggcccgccg cgatcagcgg cgaatcgtcg ccgagcagca gcttctgcac cgatgcgtca taggcggcga tcccgtcgat cggcaggtag ccgcgcggca ggccggcttc gacccgtgcc ttttccgcgt cgcgcacggc gcgcagcagc ggaatcttgc cgtcctcgtt ggtgtagacg ccgacgccca ggttcacctt ggtcgggcgc gtgtcggcgt tgaaggcttc gttcaggccc aggatagggt cgcggggggc aagttcgaca gcggagaaga gcgacatcga accaggaccc tgggtctgag cttccag
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSLFSAVEL APRDPILGLN EAFNADTRPT KVNLGVGVYT NEDGKIPLLR AVRDAEKARV EAGLPRGYLP IDGIAAYDAS VQKLLLGDDS PLIAAGRVVT AQALGGTGAL KIGADFLRTL NPKAKVAISD PSWENHRALF DMAGFEVVAY PYYDAKTNGV NFDGMLAALN GYEPGTIVVL HACCHNPTGV DLNDAQWAQV VEVVKARRLV PFLDIAYQGF GESIEADAAA VRLFAAANLN VFVSSSFSKS FSLYGERVGA LSIITDSKDE AARVLSQLKR VIRTNYSNPP THGGAIVAAV LASPELRASW VQELGEMRDR IRAMRNGLVE RLKAAGIERD FSFINAQRGM FSYSGLTSAQ VDRLREEFGI YAVSTGRICV AALNTRNLDV VANAIAAVLK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCGCTCTTC TCCGCTGTCG AACTTGCCCC CCGCGACCCT ATCCTGGGCC TGAACGAAGC CTTCAACGCC GACACGCGCC CGACCAAGGT GAACCTGGGC GTCGGCGTCT ACACCAACGA GGACGGCAAG ATTCCGCTGC TGCGCGCCGT GCGCGACGCG GAAAAGGCAC GGGTCGAAGC CGGCCTGCCG CGCGGCTACC TGCCGATCGA CGGGATCGCC GCCTATGACG CATCGGTGCA GAAGCTGCTG CTCGGCGACG ATTCGCCGCT GATCGCGGCG GGCCGCGTCG TGACGGCGCA GGCGCTCGGC GGCACGGGCG CGCTGAAGAT CGGCGCGGAT TTCCTGCGCA CCCTGAACCC GAAGGCGAAG GTGGCGATCA GCGATCCGAG CTGGGAAAAC CACCGTGCCC TGTTCGACAT GGCGGGCTTC GAAGTCGTCG CGTACCCGTA CTACGACGCG AAGACGAACG GCGTGAACTT CGACGGCATG CTGGCCGCGC TCAACGGCTA CGAGCCGGGC ACGATCGTCG TGCTGCACGC GTGCTGCCAC AACCCGACGG GCGTGGACCT GAACGATGCG CAATGGGCGC AGGTGGTCGA GGTCGTGAAG GCGCGTCGGC TCGTGCCGTT CCTGGACATC GCCTATCAAG GCTTCGGCGA AAGCATCGAG GCCGACGCGG CCGCGGTGCG CCTGTTCGCG GCGGCCAATC TGAACGTGTT CGTATCGTCG TCGTTCTCGA AGTCGTTCTC GCTGTATGGC GAGCGCGTCG GCGCGCTGTC GATCATCACC GACAGCAAGG ACGAAGCTGC ACGCGTGCTG TCGCAACTGA AGCGCGTGAT TCGCACGAAC TATTCGAACC CGCCGACGCA CGGCGGCGCG ATCGTTGCCG CGGTGCTCGC CTCGCCGGAA CTGCGCGCTT CGTGGGTGCA GGAACTGGGC GAGATGCGCG ATCGCATCCG CGCAATGCGC AACGGCCTCG TCGAGCGCCT GAAGGCGGCC GGCATCGAGC GCGACTTCAG CTTCATCAAC GCGCAACGCG GGATGTTCTC GTACTCGGGC CTGACGTCCG CGCAAGTCGA CCGTCTGCGC GAAGAATTCG GCATCTATGC GGTCAGCACC GGCCGGATCT GCGTCGCGGC GCTCAACACG CGCAACCTCG ACGTCGTCGC GAACGCGATC GCCGCGGTGT TGAAGTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA