SSGCID: Target Page

BupsE.00863.a

Putative two-component sensor kinase protein (BPSS1360)

CENTER ID:	BupsE.00863.a
ORGANISM:	Burkholderia pseudomallei K96243
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsE.00863.a.B2.GE42220	PAS and kinase domains		225	722

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272560.6.peg.5581
UniProt:	Q63KK2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQTAFWQRHL GGGEMASGIG RFGKRASRLI DFGLWAAAAL LLLTAGVGAL GTARLSDNEA DIHRASEVIS GLERLLALAR DMETSQRGYI LTGKPDYLQP YKAALPEIRK QFDALTTLVQ DDAVQQQRLA TLRMLLEKKQ HELAATIELR KTEGFDAAQA IVSSDAGKIF MDRARVMVNE MEATVQQQLR TLRDRARATR DIAIGVGLAS GLLTLAVCVS FGYVMRRLLL VEAAAAHDLF EQRELLHVTL ASIGDGVIAT DVGGRITFFN RVAEEMTALC ADAVVGQPIE RVLTFRHAAT QRVIDNPARV AVERRRTASL PAHTRFVGRS GGELPVDGNA APTFDANGKL VGAVLVVRDV SERERAEERF RLAVEAAPTA MIMVGRDGRI VLVNSQAEKL FGYARGDMIG HGIDMLVPER FRAGHWAHRD AFFGNPDARP MGEGRDLFGL RRDGSEFPVE IGLNPITTSE GAFVLCSIAD ITARKRAELE LRRRSEELAR SNQDLEQFAY VASHDLQEPL RAVAGPLQLL QRRYQGQLDA RADEFIGHAV DGATRMQSLI DDLLSYSRVG RLEDARRPVD CEHVLDEALR NLSAAIRESG AQVTHDPLPT VHGIEAQLVL LFQNLIGNAI KFRGKDRVAR IHLEAHTRDD AWLLRVRDNG IGIDPQYFER IFLIFQRLHT RREYPGTGLG LALCKRIVEH HGGRIFVESE PGQGTTFSFT LSRPGADETR WPRDPRE

NT Sequence

ttgcaaacgg ctttctggca aagacacctc ggagggggcg agatggcgtc gggcatcggg cgattcggga aaagagcgtc gcgcttgatc gatttcggtt tgtgggccgc cgccgcgctg ttactgctga cggcgggcgt cggcgcgctc ggcacggccc gcctgtccga caacgaggcg gatatccatc gcgcgagcga ggtgatatcg ggtctcgaac ggctgctggc gctggcccgc gacatggaga cgagccagcg cggatatatc ctcaccggca agccggacta ccttcagcct tacaaggcgg cactgcccga aatccgcaag cagttcgatg cgctcacgac gctcgtgcag gacgatgccg ttcagcagca gcggctcgcg acgctgcgca tgctgctcga gaaaaagcag cacgagctcg cggcgacgat cgagctgcgc aagacggagg ggttcgacgc ggcgcaggcg atcgtctcga gcgatgcggg caagatcttc atggaccgcg cgcgcgtgat ggtcaacgaa atggaggcga ccgtccagca gcaactgagg acgctgcgcg atcgcgccag ggcgacacgc gacatcgcga tcggcgtggg gctcgcgtcc gggctgctca cgctcgccgt gtgcgtatcg ttcggctatg tgatgcgccg cctgctgctc gtcgaggcgg ccgcggccca cgatctgttc gagcagcgcg aactgctgca cgtaacgctc gcgagcatcg gcgacggcgt gatcgcgacg gacgtcggcg gccgcatcac gttcttcaac cgggtcgccg aggagatgac ggcgctttgc gccgatgccg tcgtcggtca gccgatcgag cgggtgctga cttttcggca tgccgcgacg cagcgggtga tcgacaatcc ggcgcgcgtc gcggtcgagc gcaggcgcac cgcgagcttg cctgcccata cgcgcttcgt cggccgcagc ggcggggaat tgccggtgga cggcaacgcc gcaccgacgt tcgacgcgaa cggcaagctc gtcggcgcgg tgctcgtcgt gcgcgacgtg tcggagcgcg agcgggccga ggagcgattc cggctcgccg tcgaggccgc gccgaccgcg atgatcatgg tggggcgcga cggcaggatc gtgctcgtca attcgcaggc cgagaaattg ttcgggtacg cgcgcggcga catgatcggg cacggcatcg acatgctggt gcccgagcgt tttcgtgcgg ggcattgggc gcaccgcgac gcgttcttcg gcaacccgga cgcgcggccg atgggcgagg ggcgggacct gttcggtttg cgccgcgacg gcagcgagtt tccggtcgag atcggcctga atccgatcac gacctcggag ggcgcgttcg tgctgtgctc gatcgccgac atcaccgcgc gcaagcgcgc ggaactcgaa ctgcgccgca gaagcgagga gcttgcgcgc tcgaatcagg atctcgagca gttcgcctac gtcgcgtctc acgatctgca ggagccgttg cgcgcggtcg cggggcccct gcaattgctg cagcggcgat accaggggca gctcgacgcg cgggccgacg agttcatcgg gcacgcggtg gatggcgcga cgagaatgca gtcgctgatc gacgacctgc tcagctattc gcgcgtcggg cggctcgagg acgcgcgccg gccggtcgac tgcgagcacg tgctggacga ggcgctgcgc aacctgagcg cggcgattcg cgaatccggc gcgcaggtca cgcacgatcc gctgccgacc gtgcatggca tcgaggcaca gctcgtgctg ctgttccaga atctgatcgg caacgcgatc aagtttcgcg gcaaggaccg cgtcgcgcgg attcatctgg aagcgcacac gcgcgacgac gcatggctgt tgcgcgtgcg cgacaacggc atcgggatcg atccgcagta tttcgagcgc atcttcctga ttttccagcg gctgcacacg cggcgcgaat atccgggaac ggggctcggg ctcgcgctgt gcaagcggat cgtcgagcac cacggcggcc gcatcttcgt cgagtccgag ccggggcagg gcacgacgtt ttcgtttacg ctgtccaggc cgggtgccga tgaaacgcgt tggccgcgcg atccgaggga gtga

Details for BupsE.00863.a.B2.GE42220

HARVESTED ON:	2/23/2018
SEQUENCED ON:	3/1/2018
EXPECTED MW:	56kDa
OBSERVED MW:	55kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMR RLLLVEAAAA HDLFEQRELL HVTLASIGDG VIATDVGGRI TFFNRVAEEM TALCADAVVG QPIERVLTFR HAATQRVIDN PARVAVERRR TASLPAHTRF VGRSGGELPV DGNAAPTFDA NGKLVGAVLV VRDVSERERA EERFRLAVEA APTAMIMVGR DGRIVLVNSQ AEKLFGYARG DMIGHGIDML VPERFRAGHW AHRDAFFGNP DARPMGEGRD LFGLRRDGSE FPVEIGLNPI TTSEGAFVLC SIADITARKR AELELRRRSE ELARSNQDLE QFAYVASHDL QEPLRAVAGP LQLLQRRYQG QLDARADEFI GHAVDGATRM QSLIDDLLSY SRVGRLEDAR RPVDCEHVLD EALRNLSAAI RESGAQVTHD PLPTVHGIEA QLVLLFQNLI GNAIKFRGKD RVARIHLEAH TRDDAWLLRV RDNGIGIDPQ YFERIFLIFQ RLHTRREYPG TGLGLALCKR IVEHHGGRIF VESEPGQG

Validated NT Sequence

tgccctgccc cggctcggac tcgacgaaga tgcggccgcc gtggtgctcg acgatccgct tgcacagcgc gagcccgagc cccgttcccg gatattcgcg ccgcgtgtgc agccgctgga aaatcaggaa gatgcgctcg aaatactgcg gatcgatccc gatgccgttg tcgcgcacgc gcaacagcca tgcgtcgtcg cgcgtgtgcg cttccagatg aatccgcgcg acgcggtcct tgccgcgaaa cttgatcgcg ttgccgatca gattctggaa cagcagcacg agctgtgcct cgatgccatg cacggtcggc agcggatcgt gcgtgacctg cgcgccggat tcgcgaatcg ccgcgctcag gttgcgcagc gcctcgtcca gcacgtgctc gcagtcgacc ggccggcgcg cgtcctcgag ccgcccgacg cgcgaatagc tgagcaggtc gtcgatcagc gactgcattc tcgtcgcgcc atccaccgcg tgcccgatga actcgtcggc ccgcgcgtcg agctgcccct ggtatcgccg ctgcagcaat tgcaggggcc ccgcgaccgc gcgcaacggc tcctgcagat cgtgagacgc gacgtaggcg aactgctcga gatcctgatt cgagcgcgca agctcctcgc ttctgcggcg cagttcgagt tccgcgcgct tgcgcgcggt gatgtcggcg atcgagcaca gcacgaacgc gccctccgag gtcgtgatcg gattcaggcc gatctcgacc ggaaactcgc tgccgtcgcg gcgcaaaccg aacaggtccc gcccctcgcc catcggccgc gcgtccgggt tgccgaagaa cgcgtcgcgg tgcgcccaat gccccgcacg aaaacgctcg ggcaccagca tgtcgatgcc gtgcccgatc atgtcgccgc gcgcgtaccc gaacaatttc tcggcctgcg aattgacgag cacgatcctg ccgtcgcgcc ccaccatgat catcgcggtc ggcgcggcct cgacggcgag ccggaatcgc tcctcggccc gctcgcgctc cgacacgtcg cgcacgacga gcaccgcgcc gacgagcttg ccgttcgcgt cgaacgtcgg tgcggcgttg ccgtccaccg gcaattcccc gccgctgcgg ccgacgaagc gcgtatgggc aggcaagctc gcggtgcgcc tgcgctcgac cgcgacgcgc gccggattgt cgatcacccg ctgcgtcgcg gcatgccgaa aagtcagcac ccgctcgatc ggctgaccga cgacggcatc ggcgcaaagc gccgtcatct cctcggcgac ccggttgaag aacgtgatgc ggccgccgac gtccgtcgcg atcacgccgt cgccgatgct cgcgagcgtt acgtgcagca gttcgcgctg ctcgaacaga tcgtgggccg cggccgcctc gacgagcagc aggcggcgca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgcc gcctgctgct cgtcgaggcg gccgcggccc acgatctgtt cgagcagcgc gaactgctgc acgtaacgct cgcgagcatc ggcgacggcg tgatcgcgac ggacgtcggc ggccgcatca cgttcttcaa ccgggtcgcc gaggagatga cggcgctttg cgccgatgcc gtcgtcggtc agccgatcga gcgggtgctg acttttcggc atgccgcgac gcagcgggtg atcgacaatc cggcgcgcgt cgcggtcgag cgcaggcgca ccgcgagctt gcctgcccat acgcgcttcg tcggccgcag cggcggggaa ttgccggtgg acggcaacgc cgcaccgacg ttcgacgcga acggcaagct cgtcggcgcg gtgctcgtcg tgcgcgacgt gtcggagcgc gagcgggccg aggagcgatt ccggctcgcc gtcgaggccg cgccgaccgc gatgatcatg gtggggcgcg acggcaggat cgtgctcgtc aattcgcagg ccgagaaatt gttcgggtac gcgcgcggcg acatgatcgg gcacggcatc gacatgctgg tgcccgagcg ttttcgtgcg gggcattggg cgcaccgcga cgcgttcttc ggcaacccgg acgcgcggcc gatgggcgag gggcgggacc tgttcggttt gcgccgcgac ggcagcgagt ttccggtcga gatcggcctg aatccgatca cgacctcgga gggcgcgttc gtgctgtgct cgatcgccga catcaccgcg cgcaagcgcg cggaactcga actgcgccgc agaagcgagg agcttgcgcg ctcgaatcag gatctcgagc agttcgccta cgtcgcgtct cacgatctgc aggagccgtt gcgcgcggtc gcggggcccc tgcaattgct gcagcggcga taccaggggc agctcgacgc gcgggccgac gagttcatcg ggcacgcggt ggatggcgcg acgagaatgc agtcgctgat cgacgacctg ctcagctatt cgcgcgtcgg gcggctcgag gacgcgcgcc ggccggtcga ctgcgagcac gtgctggacg aggcgctgcg caacctgagc gcggcgattc gcgaatccgg cgcgcaggtc acgcacgatc cgctgccgac cgtgcatggc atcgaggcac agctcgtgct gctgttccag aatctgatcg gcaacgcgat caagtttcgc ggcaaggacc gcgtcgcgcg gattcatctg gaagcgcaca cgcgcgacga cgcatggctg ttgcgcgtgc gcgacaacgg catcgggatc gatccgcagt atttcgagcg catcttcctg attttccagc ggctgcacac gcggcgcgaa tatccgggaa cggggctcgg gctcgcgctg tgcaagcgga tcgtcgagca ccacggcggc cgcatcttcg tcgagtccga gccggggcag ggcacgacgt tttcgtttac gctgtcctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at