SSGCID: Target Page

BupsE.00559.a

Deoxyribose-phosphate aldolase (EC 4.1.2.4)

CENTER ID:	BupsE.00559.a
ORGANISM:	Burkholderia pseudomallei K96243
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsE.00559.a.A1.GE34330	full length		1	260

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272560.6.peg.6329
RefSeq:	YP_111968.1
UniProt:	Q63IV4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MELSLNRNQL TEAALKALHL IDLTSLNDDD TEERIASLAA SADTPVGTPA ALCVYPRFVG AAHAALARHG LVLPVATVTN FPHGAADPDA AARETADALA RGADEIDVVF PYRALIDGDE RVGRELVAQC RAAAGAQCLK VILETGELRD AAAIRRASEI AIEEGADFLK TSTGKVAVNA TLDAAAVMLA TIRVAGRMVG FKAAGGVRTA QDAAQYLSLA QRELSPGYLT SATFRFGASG LLGNLLETLG HRAGATRGAY

NT Sequence

atggagcttt cgttgaatcg caaccagttg accgaagcgg cgctcaaggc gctgcatctg atcgatctca cgtcgctgaa cgacgacgac accgaagaga ggatcgcctc gctcgccgcg tcggccgata cgccggtcgg cacgcccgcc gcgctgtgcg tgtatccgcg cttcgtcggc gccgcgcatg cggcgctcgc gcggcacggg ctcgtgctgc ccgtcgcgac cgtcacgaat tttccgcacg gcgcggccga tccggatgcg gccgcgcgcg agacggccga tgcgctcgcg cgcggcgcgg acgaaatcga tgtggtgttt ccgtatcgcg cgctcatcga cggcgacgaa cgggtcggcc gcgagctcgt cgcgcagtgc cgcgcggcgg cgggcgcgca gtgcctgaag gtgatcctcg agacgggcga gctgcgcgac gcggcggcga tccgccgcgc gagcgagatc gcgatcgagg agggcgcgga ttttctgaag acgtcgacgg gcaaggtcgc ggtgaacgcg acgctcgacg cggcggccgt gatgctcgcg acgatccgcg tcgcgggccg catggtcggc ttcaaggcgg cgggcggcgt gcgcaccgcg caggacgcgg cgcagtacct gtcgctcgcg cagcgcgagc tcagcccggg ctatctgacg agcgcgacgt ttcgcttcgg cgcgtcgggg ctgctcggca atctgctcga gacgctcggg caccgggcag gcgcgacgcg cggcgcgtat tga

Details for BupsE.00559.a.A1.GE34330

HARVESTED ON:	1/10/2012
SEQUENCED ON:	1/13/2012
EXPECTED MW:	29kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMELSLNRNQ LTEAALKALH LIDLTSLNDD DTEERIASLA ASADTPVGTP AALCVYPRFV GAAHAALARH GLVLPVATVT NFPHGAADPD AAARETADAL ARGADEIDVV FPYRALIDGD ERVGRELVAQ CRAAAGAQCL KVILETGELR DAAAIRRASE IAIEEGADFL KTSTGKVAVN ATLDAAAVML ATIRVAGRMV GFKAAGGVRT AQDAAQYLSL AQRELSPGYL TSATFRFGAS GLLGNLLETL GHRAGATRGA Y

Validated NT Sequence

ttattaatac gcgccgcgcg tcgcgcctgc ccggtgcccg agcgtctcga gcagattgcc gagcagcccc gacgcgccga agcgaaacgt cgcgctcgtc agatagcccg ggctgagctc gcgctgcgcg agcgacaggt actgcgccgc gtcctgcgcg gtgcgcacgc cgcccgccgc cttgaagccg accatgcggc ccgcgacgcg gatcgtcgcg agcatcacgg ccgccgcgtc gagcgtcgcg ttcaccgcga ccttgcccgt cgacgtcttc agaaaatccg cgccctcctc gatcgcgatc tcgctcgcgc ggcggatcgc cgccgcgtcg cgcagctcgc ccgtctcgag gatcaccttc aggcactgcg cgcccgccgc cgcgcggcac tgcgcgacga gctcgcggcc gacccgttcg tcgccgtcga tgagcgcgcg atacggaaac accacatcga tttcgtccgc gccgcgcgcg agcgcatcgg ccgtctcgcg cgcggccgca tccggatcgg ccgcgccgtg cggaaaattc gtgacggtcg cgacgggcag cacgagcccg tgccgcgcga gcgccgcatg cgcggcgccg acgaagcgcg gatacacgca cagcgcggcg ggcgtgccga ccggcgtatc ggccgacgcg gcgagcgagg cgatcctctc ttcggtgtcg tcgtcgttca gcgacgtgag atcgatcaga tgcagcgcct tgagcgccgc ttcggtcaac tggttgcgat tcaacgaaag ctccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggagctttcg ttgaatcgca accagttgac cgaagcggcg ctcaaggcgc tgcatctgat cgatctcacg tcgctgaacg acgacgacac cgaagagagg atcgcctcgc tcgccgcgtc ggccgatacg ccggtcggca cgcccgccgc gctgtgcgtg tatccgcgct tcgtcggcgc cgcgcatgcg gcgctcgcgc ggcacgggct cgtgctgccc gtcgcgaccg tcacgaattt tccgcacggc gcggccgatc cggatgcggc cgcgcgcgag acggccgatg cgctcgcgcg cggcgcggac gaaatcgatg tggtgtttcc gtatcgcgcg ctcatcgacg gcgacgaacg ggtcggccgc gagctcgtcg cgcagtgccg cgcggcggcg ggcgcgcagt gcctgaaggt gatcctcgag acgggcgagc tgcgcgacgc ggcggcgatc cgccgcgcga gcgagatcgc gatcgaggag ggcgcggatt ttctgaagac gtcgacgggc aaggtcgcgg tgaacgcgac gctcgacgcg gcggccgtga tgctcgcgac gatccgcgtc gcgggccgca tggtcggctt caaggcggcg ggcggcgtgc gcaccgcgca ggacgcggcg cagtacctgt cgctcgcgca gcgcgagctc agcccgggct atctgacgag cgcgacgttt cgcttcggcg cgtcggggct gctcggcaat ctgctcgaga cgctcgggca ccgggcaggc gcgacgcgcg gcgcgtatta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a