SSGCID: Target Page

BupsE.00203.a

Pyridoxine/pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5) (PNP/PMP oxidase) (PNPOx) (Pyridoxal 5'-phosphate synthase)

CENTER ID:	BupsE.00203.a
ORGANISM:	Burkholderia pseudomallei K96243
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsE.00203.a.A1.GE34326	full length		1	214

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272560.6.peg.922
RefSeq:	YP_107479.2
UniProt:	Q63WN7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTTLADLRTN YSRASLDAAD VNPNPFVQFD VWFKEALDAQ LPEPNTMTLA TVDESGRPSA RIVLIKGADE RGFVFFTNYE SRKGRELAHN PNAALLFYWI ELERQVRVEG RIEKTSEEES DRYFASRPLG SRIGAWASEQ SAVIESRALL EAREKEIGAR FGENPPRPPH WGGYRLVPSS IEFWQGRPSR LHDRLLYTRD AASASGWKIA RLAP

NT Sequence

ttgagcgttc gcacaccaat gacgacactt gccgatctcc gcaccaacta ctcccgcgcg tcgctcgacg cggcggacgt gaacccgaat ccgttcgtcc agttcgacgt ctggttcaag gaagcgctcg acgcgcaact gcccgagccc aacacgatga cgctcgcgac cgtcgacgaa tccggccggc cgtccgcgcg gatcgtcctc atcaagggcg ccgacgagcg cggcttcgtg ttcttcacga actacgaaag ccgcaagggg cgcgagctcg cgcacaaccc gaacgcggcg ctgctcttct actggatcga actcgagcgc caggtgcgcg tcgaggggcg catcgagaag acgagcgaag aggaaagcga ccggtatttc gcgtcgcggc cgctcggctc gcggatcggc gcatgggcgt ccgagcagag cgcggtgatc gagagccgcg cgctgctcga ggcgcgcgag aaggaaatcg gcgcgcgctt cggcgagaac ccgccgcgcc cgccgcactg gggcggctac cgtctcgtgc cgagctcgat cgaattctgg cagggccgcc cgtcgcggct ccacgaccgc ctgctgtata cgcgcgacgc cgcgtcggcg agcggctgga agatcgcgcg cctcgcgccg taa

Details for BupsE.00203.a.A1.GE34326

HARVESTED ON:	1/10/2012
SEQUENCED ON:	1/13/2012
EXPECTED MW:	26kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	88
PERCENT COVERAGE:	93

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLSVRTPMT TLADLRTNYS RASLDAADVN PNPFVQFDVW FKEALDAQLP EPNTMTLATV DESGRPSARI VLIKGADERG FVFFTNYESR KGRELAHNPN AALLFYWIEL ERQVRVEGRI EKTSEEESDR XFASRPLGSR IGAWASEQSA VIXSRALLXA RXKEIGARFX XXPPRPPXWG XXXXRXXXXX XXGXGXPXRX X

Validated NT Sequence

ttnttnnnct ttnaagaaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttga gcgttcgcac accaatgacg acacttgccg atctccgcac caactactcc cgcgcgtcgc tcgacgcggc ggacgtgaac ccgaatccgt tcgtccagtt cgacgtctgg ttcaaggaag cgctcgacgc gcaactgccc gagcccaaca cgatgacgct cgcgaccgtc gacgaatccg gccggccgtc cgcgcggatc gtcctcatca agggcgccga cgagcgcggc ttcgtgttct tcacgaacta cgaaagccgc aaggggcgcg agctcgcgca caacccgaac gcggcgctgc tcttctactg gatcgaactc gagcgccagg tgcgcgtcga ggggcgcatc gagaagacga gcgaagagga aagcgaccgg natttcgcgt cgcggccgct cggctcgcgg atcggcgcat gggcgtccga gcagagcgcg gtgatcnaga gccgcgcgct gctcnaggcg cgcganaagg aaatcggcgc gcgcttcngc nannnnccgc cgcgcccgcc gcantggggn gnntnnnnnn nncgngnnnn gntcnnnnnn nnnnntggna ngggcnnncc gnngcgnntn cnnc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLSVRTPMT TLADLRTNYS RASLDAADVN PNPFVQFDVW FKEALDAQLP EPNTMTLATV DESGRPSARI VLIKGADERG FVFFTNYESR KGRELAHNPN AALLFYWIEL ERQVRVEGRI EKTSEEESDR YFASRPLGSR IGAWASEQSA VIESRALLEA REKEIGARFG ENPPRPPHWG GYRLVPSSIE FWQGRPSRLH DRLLYTRDAA SASGWK

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgagcgtt cgcacaccaa tgacgacact tgccgatctc cgcaccaact actcccgcgc gtcgctcgac gcggcggacg tgaacccgaa tccgttcgtc cagttcgacg tctggttcaa ggaagcgctc gacgcgcaac tgcccgagcc caacacgatg acgctcgcga ccgtcgacga atccggccgg ccgtccgcgc ggatcgtcct catcaagggc gccgacgagc gcggcttcgt gttcttcacg aactacgaaa gccgcaaggg gcgcgagctc gcgcacaacc cgaacgcggc gctgctcttc tactggatcg aactcgagcg ccaggtgcgc gtcgaggggc gcatcgagaa gacgagcgaa gaggaaagcg accggtattt cgcgtcgcgg ccgctcggct cgcggatcgg cgcatgggcg tccgagcaga gcgcggtgat cgagagccgc gcgctgctcg aggcgcgcga gaaggaaatc ggcgcgcgct tcggcgagaa cccgccgcgc ccgccgcact ggggcggcta ccgtctcgtg ccgagctcga tcgaattctg gcagggccgc ccgtcgcggc tccacgaccg cctgctgtat acgcgcgacg ccgcgtcggc gagcggctgg aagtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa