CENTER ID: | BupsD.18988.a |
ORGANISM: | Burkholderia pseudomallei 668 |
ASSOCIATED DISEASE: | Melioidosis |
CURRENT STATUS: | HSQC satisfactory |
COMMUNITY REQUEST: | True |
NIH RISK GROUP: | 3 |
SELECT AGENT: | True |
NIH PRIORITY pathogens category: |
IIIB |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
BupsD.18988.a.D2.GE00001 | mature protein | 22 | 113 | ||
BupsD.18988.a.DN2.GE41370 | mature protein | 22 | 113 |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
BupsD.18988.a.DN2.PS38223 | mature protein | 22 | 113 |
RESOURCE | REFERENCE ID |
---|---|
PATRIC ID: | fig|320373.5.peg.6271 |
RefSeq: | YP_001063863.1 |
UniProt: | A3NNE2 |
HARVESTED ON: | 8/31/2016 |
SEQUENCED ON: | 5/27/2016 |
EXPECTED MW: | 21kDa |
OBSERVED MW: | 21kDa |
ANTIBIOTIC MARKER: | kanamycin |
COUNT OF EXPRESSION COLONIES: | Many (50-100) |
TOTAL EXPRESSION LEVEL: | Moderate Expression |
SOLUBLE EXPRESSION LEVEL | Low Expression |
EXPRESSION HOST: | Rosetta2 (DE3) |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | 99 |
PERCENT COVERAGE: | 100 |
HARVESTED ON: | 2/2/2017 |
SEQUENCED ON: | 1/23/2017 |
EXPECTED MW: | 14kDa |
OBSERVED MW: | 15kDa |
ANTIBIOTIC MARKER: | kanamycin |
COUNT OF EXPRESSION COLONIES: | Good (10-50) |
TOTAL EXPRESSION LEVEL: | Moderate Expression |
SOLUBLE EXPRESSION LEVEL | Moderate Expression |
EXPRESSION HOST: | BL 21 (DE3) Rosetta |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | 98 |
PERCENT COVERAGE: | 89 |
PURIFICATION DATe: | 3/17/2017 |
CONCENTRATION: | 5.3mg/ml |
OBSERVED MW: | 15kDa |
EXPRESSION LEVEL: | Moderate Expression |
PROTEIN PURIFICATION BUFFER: | SEC Buffer: 20mM HEPES pH 7.0, 300mM NaCl, 5% glycerol, 1mM TCEP |
EXPRESSION HOST: | BL 21 (DE3) Rosetta |
VIAL COUNT (approx.): | 9 |
VIAL VOLUME: | 110µl |
PERCENT IDENTITY: | 98 |
PERCENT COVERAGE: | 89 |