SSGCID: Target Page

BupsA.17781.a

Polysaccharide deacetylase family protein

CENTER ID:	BupsA.17781.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17781.a.A1.GE31542	Full length( BupsA.17781.a )		1	298

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.2514
RefSeq:	YP_333800.1
UniProt:	Q3JRK3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MARIVLKIDV DTLRGTREGV PNLARIFDRF AARATFLFSL GPDHTGWAMR RVLRPGFLKK VSRTSVVEHY GVRQLLYGVL LPGPDIGRRA RADMRAIHEA GFECGIHTWD HVYWQDNVRR RPREWTVREM NKSRERFVEI FGAPPVTHGA AGWQMNDAAF EQIDAWGMRY ASDGRGHSPY LPVVAGKTLA HVQMPTTLPT LDEILGVDGI DTGNVAARVL EHTERNPHDQ VFTLHAELEG QKLAPVFEQL LAGWRAQGHT FATMGDYYAT LDRAALPSYP VAWGEIPGRS GELIVQPD

NT Sequence

TTGGCTCGCA TCGTCCTCAA GATCGACGTC GACACGTTGC GCGGCACGCG CGAAGGCGTG CCGAACCTCG CGCGCATCTT CGATCGCTTC GCCGCGCGCG CGACCTTCCT CTTCAGCCTC GGCCCCGATC ACACCGGCTG GGCGATGCGC CGCGTGCTGC GTCCCGGGTT CCTGAAGAAG GTGTCGCGCA CGTCGGTCGT CGAACATTAC GGCGTCAGGC AACTGCTGTA CGGCGTGCTG CTGCCCGGCC CCGACATCGG CCGCCGCGCG AGAGCCGACA TGCGCGCGAT CCACGAAGCC GGCTTCGAAT GCGGCATCCA CACGTGGGAT CACGTGTACT GGCAGGACAA CGTCCGCCGG CGCCCGCGCG AGTGGACCGT GCGCGAGATG AACAAGAGCC GCGAGCGCTT CGTCGAGATC TTCGGCGCGC CGCCCGTCAC GCACGGCGCG GCCGGCTGGC AGATGAACGA CGCGGCGTTC GAGCAGATCG ACGCGTGGGG GATGCGCTAC GCGTCGGACG GCCGCGGCCA TTCGCCGTAT CTGCCCGTCG TCGCGGGCAA AACGCTCGCG CACGTGCAGA TGCCGACCAC GCTGCCGACG CTCGACGAGA TCCTCGGCGT CGACGGCATC GATACGGGCA ACGTCGCCGC GCGTGTGCTC GAGCATACCG AGCGCAATCC GCACGACCAG GTGTTCACGC TGCACGCGGA GCTCGAAGGC CAGAAGCTCG CGCCGGTGTT CGAGCAACTG CTCGCGGGCT GGCGCGCGCA AGGCCACACG TTCGCGACGA TGGGCGACTA TTACGCGACG CTGGACCGCG CCGCGCTGCC ATCGTACCCT GTCGCGTGGG GCGAAATCCC CGGCCGCTCC GGCGAGCTGA TCGTCCAGCC CGAC

Details for BupsA.17781.a.A1.GE31542

HARVESTED ON:	2/1/2011
SEQUENCED ON:	2/8/2011
EXPECTED MW:	35kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	63
PERCENT COVERAGE:	82

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLARIVLKI DVDTLRGTRE GVPNLARIFD RFAARATFLF SLGPDHTGWA MRRVLRPGFL KKVSRTSVVE HYGVRQLLYG VLLPGPDIGR RARADMRAIH EAGFECGIHT WDHVYWQDNV RRRPREWTVR EMNKXRERFV XIXGXRHARX GXXXXXRXGX RXXRXXGXXL XXXXXXXXXX SXXXXGXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXX

Validated NT Sequence

cnnnnnnnnn nnnnnnnnnn nnnnngnnnt nnnnnnnngn nnnngattac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgttg gctcgcatcg tcctcaagat cgacgtcgac acgttgcgcg gcacgcgcga aggcgtgccg aacctcgcgc gcatcttcga tcgcttcgcc gcgcgcgcga ccttcctctt cagcctcggc cccgatcaca ccggctgggc gatgcgccgc gtgctgcgtc ccgggttcct gaagaaggtg tcgcgcacgt cggtcgtcga acattacggc gtcaggcaac tgctgtacgg cgtgctgctg cccggccccg acatcggccg ccgcgcgaga gccgacatgc gcgcgatcca cgaagccggc ttcgaatgcg gcatccacac gtgggatcac gtgtactggc aggacaacgt ccgccggcgc ccgcgcgagt ggaccgtgcg cgagatgaac aaganccgcg agcgcttcgt cnagatcntc ggcgnccgtc acgcacggng cggccngntn gnananganc gacncggnnn tcgancanat cgacncnngg ggnangnnct acnnntngna nngnnnnnnn cattnnncnn atcngncnnn nnnnncgggn naaannnnnn nnnnngtgnc nnnnnnnnnn nnannntngn nnnnnnnnnn nnnnnnnnnn nnntnncngn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cnnnnnnnnn nnnnnnnnnn annnnnnnnn nnnnnngnnn nnncnnnnnn nnnnnnnnnn nngggnnnnn c

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLARIVLKI DVDTLRGTRE GVPNLARIFD RFAARATFLF SLGPDHTGWA MRRVLRPGFL KKVSRTSVVE HYGVRQLLYG VLLPGPDIGR RARADMRAIH EAGFECGIHT WDHVYWQDNV RRRPREWTVR EMNKSRERFV EIFGAPPVTH GAAGWQMNDA AFEQIDAWGM RYASDGRGHS PYLPVVAGKT LAHVQMPTTL PTLDEILGVD GIDTGNVAAR VLEHTERNPH DQVFTLHAEL EGQKLAPVFE QLLAGWRAQG HTFATMGDYY ATLDRAALPS YPVAWGEIPG RSGELIVQPD

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttggctcgc atcgtcctca agatcgacgt cgacacgttg cgcggcacgc gcgaaggcgt gccgaacctc gcgcgcatct tcgatcgctt cgccgcgcgc gcgaccttcc tcttcagcct cggccccgat cacaccggct gggcgatgcg ccgcgtgctg cgtcccgggt tcctgaagaa ggtgtcgcgc acgtcggtcg tcgaacatta cggcgtcagg caactgctgt acggcgtgct gctgcccggc cccgacatcg gccgccgcgc gagagccgac atgcgcgcga tccacgaagc cggcttcgaa tgcggcatcc acacgtggga tcacgtgtac tggcaggaca acgtccgccg gcgcccgcgc gagtggaccg tgcgcgagat gaacaagagc cgcgagcgct tcgtcgagat cttcggcgcg ccgcccgtca cgcacggcgc ggccggctgg cagatgaacg acgcggcgtt cgagcagatc gacgcgtggg ggatgcgcta cgcgtcggac ggccgcggcc attcgccgta tctgcccgtc gtcgcgggca aaacgctcgc gcacgtgcag atgccgacca cgctgccgac gctcgacgag atcctcggcg tcgacggcat cgatacgggc aacgtcgccg cgcgtgtgct cgagcatacc gagcgcaatc cgcacgacca ggtgttcacg ctgcacgcgg agctcgaagg ccagaagctc gcgccggtgt tcgagcaact gctcgcgggc tggcgcgcgc aaggccacac gttcgcgacg atgggcgact attacgcgac gctggaccgc gccgcgctgc catcgtaccc tgtcgcgtgg ggcgaaatcc ccggccgctc cggcgagctg atcgtccagc ccgactaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa