SSGCID: Target Page

BupsA.17764.a

Transcriptional regulator ModE

CENTER ID:	BupsA.17764.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17764.a.A1.GE31534	Full length( BupsA.17764.a )		1	282

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17764.a.A1.PS01156	Full length( BupsA.17764.a )		1	282

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.4917
RefSeq:	YP_336029.1
UniProt:	Q3JK74

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTADSLSSRS DARAARESLA LSGELWLHAG GQTLGGAARI ALLAAIGETG SITRAAKAVG LSYKGAWDAI DTMNNLAGEP LVLRATGGKG GGGTTLTPRA TALIAAFRAI EREHRRFIDA ASAAVEGFEV NWKLIGRIGM KTSARNQLFG KVLAVKHGAV NDEVVLALPG EHTITAVVTH ESVQELGLAP GVDACALVKA SWVVLAVEDG SPLRLSARNQ LQGVVETVTR GVVNSEVLLA LDGGMTLAAI VTNDSVDALG LAQGVSAVAA FKASSVILAV NG

NT Sequence

ATGACCGCCG ATTCGCTCTC CTCCCGTTCC GATGCGCGCG CCGCGCGCGA GTCGCTCGCG TTGAGCGGCG AGCTGTGGCT GCACGCGGGC GGGCAGACGC TCGGCGGCGC GGCGCGCATC GCGCTGCTCG CCGCGATCGG CGAGACCGGA TCGATCACGC GCGCGGCGAA GGCGGTCGGC CTCAGCTACA AAGGCGCGTG GGACGCGATC GACACGATGA ACAACCTCGC GGGCGAGCCG CTCGTGCTGC GCGCGACGGG CGGCAAGGGC GGCGGCGGCA CGACGCTCAC GCCGCGCGCG ACCGCGCTGA TCGCGGCGTT TCGCGCGATC GAGCGCGAGC ATCGGCGCTT TATCGACGCG GCGAGCGCCG CGGTCGAAGG CTTCGAGGTC AATTGGAAAC TTATCGGGAG AATCGGCATG AAGACGAGCG CACGCAACCA ACTGTTCGGC AAGGTCCTGG CCGTCAAGCA CGGCGCGGTG AACGACGAAG TGGTGCTCGC GTTGCCGGGC GAACACACGA TCACGGCAGT GGTGACGCAC GAGAGCGTGC AGGAGCTGGG GCTCGCGCCG GGCGTCGACG CGTGTGCGCT CGTGAAGGCG TCGTGGGTCG TGCTCGCGGT CGAGGACGGC TCGCCGCTCA GGCTATCGGC GCGCAATCAA TTGCAGGGCG TCGTCGAGAC GGTCACGCGC GGCGTGGTGA ACAGCGAGGT GCTGCTCGCG CTCGACGGCG GCATGACGCT CGCGGCGATC GTCACGAACG ACAGCGTCGA TGCGCTCGGG CTCGCGCAGG GCGTGAGCGC GGTGGCGGCG TTCAAGGCGT CGAGCGTGAT TCTCGCGGTC AACGGA

Details for BupsA.17764.a.A1.GE31534

HARVESTED ON:	2/1/2011
SEQUENCED ON:	2/8/2011
EXPECTED MW:	31kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	95

Validated AA Sequence

MGTLEAQTQG PGSMTADSLS SRSDARAARE SLALSGELWL HAGGQTLGGA ARIALLAAIG ETGSITRAAK AVGLSYKGAW DAIDTMNNLA GEPLVLRATG GKGGGGTTLT PRATALIAAF RAIEREHRRF IDAASAAVEG FEVNWKLIGR IGMKTSARNQ LFGKVLAVKH GAVNDEVVLA LPGEHTITAV VTHESVQELG LAPGVDACAL VKASWVVLAV EDGSPLRLSA RNQLQGVVET VTRGVVNSEV LLALDGGMTL AAIVTNDSVD ALGLAQGVSA VAAFKASSVI

Validated NT Sequence

gagaatcacg ctcgacgcct tgaacgccgc caccgcgctc acgccctgcg cgagcccgag cgcatcgacg ctgtcgttcg tgacgatcgc cgcgagcgtc atgccgccgt cgagcgcgag cagcacctcg ctgttcacca cgccgcgcgt gaccgtctcg acgacgccct gcaattgatt gcgcgccgat agcctgagcg gcgagccgtc ctcgaccgcg agcacgaccc acgacgcctt cacgagcgca cacgcgtcga cgcccggcgc gagccccagc tcctgcacgc tctcgtgcgt caccactgcc gtgatcgtgt gttcgcccgg caacgcgagc accacttcgt cgttcaccgc gccgtgcttg acggccagga ccttgccgaa cagttggttg cgtgcgctcg tcttcatgcc gattctcccg ataagtttcc aattgacctc gaagccttcg accgcggcgc tcgccgcgtc gataaagcgc cgatgctcgc gctcgatcgc gcgaaacgcc gcgatcagcg cggtcgcgcg cggcgtgagc gtcgtgccgc cgccgccctt gccgcccgtc gcgcgcagca cgagcggctc gcccgcgagg ttgttcatcg tgtcgatcgc gtcccacgcg cctttgtagc tgaggccgac cgccttcgcc gcgcgcgtga tcgatccggt ctcgccgatc gcggcgagca gcgcgatgcg cgccgcgccg ccgagcgtct gcccgcccgc gtgcagccac agctcgccgc tcaacgcgag cgactcgcgc gcggcgcgcg catcggaacg ggaggagagc gaatcggcgg tcatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTADSLSSR SDARAARESL ALSGELWLHA GGQTLGGAAR IALLAAIGET GSITRAAKAV GLSYKGAWDA IDTMNNLAGE PLVLRATGGK GGGGTTLTPR ATALIAAFRA IEREHRRFID AASAAVEGFE VNWKLIGRIG MKTSARNQLF GKVLAVKHGA VNDEVVLALP GEHTITAVVT HESVQELGLA PGVDACALVK ASWVVLAVED GSPLRLSARN QLQGVVETVT RGVVNSEVLL ALDGGMTLAA IVTNDSVDAL GLAQGVSAVA AFKASSVILA VNG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgccgat tcgctctcct cccgttccga tgcgcgcgcc gcgcgcgagt cgctcgcgtt gagcggcgag ctgtggctgc acgcgggcgg gcagacgctc ggcggcgcgg cgcgcatcgc gctgctcgcc gcgatcggcg agaccggatc gatcacgcgc gcggcgaagg cggtcggcct cagctacaaa ggcgcgtggg acgcgatcga cacgatgaac aacctcgcgg gcgagccgct cgtgctgcgc gcgacgggcg gcaagggcgg cggcggcacg acgctcacgc cgcgcgcgac cgcgctgatc gcggcgtttc gcgcgatcga gcgcgagcat cggcgcttta tcgacgcggc gagcgccgcg gtcgaaggct tcgaggtcaa ttggaaactt atcgggagaa tcggcatgaa gacgagcgca cgcaaccaac tgttcggcaa ggtcctggcc gtcaagcacg gcgcggtgaa cgacgaagtg gtgctcgcgt tgccgggcga acacacgatc acggcagtgg tgacgcacga gagcgtgcag gagctggggc tcgcgccggg cgtcgacgcg tgtgcgctcg tgaaggcgtc gtgggtcgtg ctcgcggtcg aggacggctc gccgctcagg ctatcggcgc gcaatcaatt gcagggcgtc gtcgagacgg tcacgcgcgg cgtggtgaac agcgaggtgc tgctcgcgct cgacggcggc atgacgctcg cggcgatcgt cacgaacgac agcgtcgatg cgctcgggct cgcgcagggc gtgagcgcgg tggcggcgtt caaggcgtcg agcgtgattc tcgcggtcaa cggataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for BupsA.17764.a.A1.PS01156

PURIFICATION DATe:	7/20/2011
CONCENTRATION:	36.85mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	95

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCGCCGAT TCGCTCTCCT CCCGTTCCGA TGCGCGCGCC GCGCGCGAGT CGCTCGCGTT GAGCGGCGAG CTGTGGCTGC ACGCGGGCGG GCAGACGCTC GGCGGCGCGG CGCGCATCGC GCTGCTCGCC GCGATCGGCG AGACCGGATC GATCACGCGC GCGGCGAAGG CGGTCGGCCT CAGCTACAAA GGCGCGTGGG ACGCGATCGA CACGATGAAC AACCTCGCGG GCGAGCCGCT CGTGCTGCGC GCGACGGGCG GCAAGGGCGG CGGCGGCACG ACGCTCACGC CGCGCGCGAC CGCGCTGATC GCGGCGTTTC GCGCGATCGA GCGCGAGCAT CGGCGCTTTA TCGACGCGGC GAGCGCCGCG GTCGAAGGCT TCGAGGTCAA TTGGAAACTT ATCGGGAGAA TCGGCATGAA GACGAGCGCA CGCAACCAAC TGTTCGGCAA GGTCCTGGCC GTCAAGCACG GCGCGGTGAA CGACGAAGTG GTGCTCGCGT TGCCGGGCGA ACACACGATC ACGGCAGTGG TGACGCACGA GAGCGTGCAG GAGCTGGGGC TCGCGCCGGG CGTCGACGCG TGTGCGCTCG TGAAGGCGTC GTGGGTCGTG CTCGCGGTCG AGGACGGCTC GCCGCTCAGG CTATCGGCGC GCAATCAATT GCAGGGCGTC GTCGAGACGG TCACGCGCGG CGTGGTGAAC AGCGAGGTGC TGCTCGCGCT CGACGGCGGC ATGACGCTCG CGGCGATCGT CACGAACGAC AGCGTCGATG CGCTCGGGCT CGCGCAGGGC GTGAGCGCGG TGGCGGCGTT CAAGGCGTCG AGCGTGATTC TCGCGGTCAA CGGATAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA