SSGCID: Target Page

BupsA.17748.a

MOSC domain protein

CENTER ID:	BupsA.17748.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17748.a.A1.GE31529	Full length( BupsA.17748.a )		1	229

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.1169
RefSeq:	YP_332571.1
UniProt:	Q3JV32

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MHPTTAKLDA LLTGAIRPLA GTARTSAIDK QPVRERSWLA RTGFAGDEQA DRKHHGGPDK AVHHYAFDHY PQWIAEIGPR DVLARPGAFG ENLSTRGVTE RDVCVGDVFT LGGATLQVSQ SRQPCWKLNA RFGLPKMSAR VQQSGRTGWY YRVLDEGWVE PDAELALRER PYPQWPLSNV LDVLYRRTLD VGALEALSRI DALPPSWRQL FERRLKAGAV EDWTKRLDE

NT Sequence

ATGCATCCGA CAACCGCGAA GCTCGATGCG CTGCTGACCG GTGCGATACG GCCGCTCGCG GGCACGGCAC GCACGAGCGC GATCGACAAG CAGCCGGTGC GCGAGCGCTC ATGGCTCGCC CGCACCGGCT TCGCCGGCGA CGAGCAGGCC GATCGCAAGC ATCACGGCGG CCCGGACAAG GCGGTCCATC ACTATGCGTT CGATCACTAT CCGCAGTGGA TCGCCGAGAT CGGTCCGCGC GACGTGCTGG CCCGGCCCGG CGCATTCGGC GAGAACCTGT CGACGCGCGG CGTCACCGAA CGCGACGTGT GCGTCGGCGA TGTCTTCACG CTCGGCGGCG CGACGCTGCA GGTGTCGCAA TCCCGGCAGC CGTGCTGGAA GCTCAACGCA CGCTTCGGAC TGCCGAAGAT GTCCGCGCGC GTGCAGCAGA GCGGGCGAAC CGGCTGGTAC TACCGCGTGC TCGACGAAGG CTGGGTGGAG CCCGACGCCG AGCTCGCGTT GCGCGAGCGG CCGTATCCGC AATGGCCGCT GTCGAACGTG CTCGACGTGC TGTACCGTCG CACGCTCGAC GTCGGCGCGC TCGAAGCGCT ATCGCGCATC GACGCGCTGC CGCCGTCATG GCGCCAGTTG TTCGAGCGGC GGCTGAAGGC GGGCGCGGTC GAGGACTGGA CGAAGCGGCT CGACGAA

Details for BupsA.17748.a.A1.GE31529

HARVESTED ON:	2/1/2011
SEQUENCED ON:	2/8/2011
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	17
PERCENT COVERAGE:	88

Validated AA Sequence

MXLXHHXXXX GYPGSSDPGS WFDASDNREA RCAADRCDTA ARGHGTHERD RQAAGARALM ARPHRLRRRR AGRSQASRRP GQGGPSLCVR SLSAVDRRDR SARRAGPARR IRREPVDARR HRTRRVRRRC LHARRRDAAG VAIPAAVLEA QRTLXTXXXX XXXSXXXXXP XGTXXXXXXK XXXXXXXPXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XX

Validated NT Sequence

tannnnnnnn nnnnactnnn nnnnnggngt anatnaccna tgngnctcnt ncaccatcnc cntnnanntg ggtaccctgg aagctcagac ccagggtcct ggttcgatgc atccgacaac cgcgaagctc gatgcgctgc tgaccggtgc gatacggccg ctcgcgggca cggcacgcac gagcgcgatc gacaagcagc cggtgcgcga gcgctcatgg ctcgcccgca ccggcttcgc cggcgacgag caggccgatc gcaagcatca cggcggcccg gacaaggcgg tccatcacta tgcgttcgat cactatccgc agtggatcgc cgagatcggt ccgcgcgacg tgctggcccg gcccggcgca ttcggcgaga acctgtcgac gcgcggcgtc accgaacgcg acgtgtgcgt cggcgatgtc ttcacgctcg gcggcgcgac gctgcaggtg tcgcaatccc ggcagccgtg ctggaagctc aacgcacgct tcnnactgnn naannnntcc nnncnnnntc agnnannngg gnnnaaccng ntggnacnnn ccnnnnnnnn ncnnnnaagg nnnggnnggn nncccnnnnc ccnnnntnnn nntnnnnnnn nnngtncnnn nnngnnnnnn nnnnaannnn nnnnnnngnn nnnnnnnnnn nnntnnnnnn nncnnnnann nnnnnnnnnn nnnnnnna

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMHPTTAKLD ALLTGAIRPL AGTARTSAID KQPVRERSWL ARTGFAGDEQ ADRKHHGGPD KAVHHYAFDH YPQWIAEIGP RDVLARPGAF GENLSTRGVT ERDVCVGDVF TLGGATLQVS QSRQPCWKLN ARFGLPKMSA RVQQSGRTGW YYRVLDEGWV EPDAELALRE RPYPQWPLSN VLDVLYRRTL DVGALEALSR IDALPPSWRQ LFERRLKAGA VEDWTKRLDE

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcatccgaca accgcgaagc tcgatgcgct gctgaccggt gcgatacggc cgctcgcggg cacggcacgc acgagcgcga tcgacaagca gccggtgcgc gagcgctcat ggctcgcccg caccggcttc gccggcgacg agcaggccga tcgcaagcat cacggcggcc cggacaaggc ggtccatcac tatgcgttcg atcactatcc gcagtggatc gccgagatcg gtccgcgcga cgtgctggcc cggcccggcg cattcggcga gaacctgtcg acgcgcggcg tcaccgaacg cgacgtgtgc gtcggcgatg tcttcacgct cggcggcgcg acgctgcagg tgtcgcaatc ccggcagccg tgctggaagc tcaacgcacg cttcggactg ccgaagatgt ccgcgcgcgt gcagcagagc gggcgaaccg gctggtacta ccgcgtgctc gacgaaggct gggtggagcc cgacgccgag ctcgcgttgc gcgagcggcc gtatccgcaa tggccgctgt cgaacgtgct cgacgtgctg taccgtcgca cgctcgacgt cggcgcgctc gaagcgctat cgcgcatcga cgcgctgccg ccgtcatggc gccagttgtt cgagcggcgg ctgaaggcgg gcgcggtcga ggactggacg aagcggctcg acgaataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa