BupsA.17672.a

Putative exported protein

CENTER ID: BupsA.17672.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17672.a.A1.GE31496 Full length( BupsA.17672.a ) 1 355
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.3100
RefSeq: YP_334349.1
UniProt: Q3JQ04

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATAPAASAA LPIDELRVPP GFRVQVLADD VPTAREMAWS PRGILYVGSM NGRVHALVVR DGHVREHHVI ASGLEMPVGV AYRSGALFVS AVSRILRLDR IDERLAAPPK PVVVTNALPT DRHHGWKFIA FGPDGKLYVP TGAPCNICVA DRDRYAMIGR MNADGSGYEV YARGVRNTVG FAWHPATREL WFTDNGRDLM GDDRPDDKLN RAPRAGLDFG YPFCHGGDVL DPQFGRGHTC SSYAPPVLKL GAHVAALGMR FYTGGMFPPE YRDNIFIAEH GSWNRSRKVG YRVVRVIASP DGRAAREETF VHGWLRPDES VWGRPADVLP LPDGSLLVSD DYAGAIYRIT YDATH
NT Sequence
GTGGCCACGG CCCCCGCCGC GTCGGCCGCA TTGCCGATCG ACGAGCTCCG CGTGCCGCCC GGATTCCGGG TCCAGGTGCT CGCCGACGAC GTGCCCACCG CGCGCGAGAT GGCGTGGTCG CCGCGCGGCA TCCTCTACGT CGGCAGCATG AACGGACGAG TGCACGCGCT CGTCGTGCGC GACGGCCATG TCCGCGAACA CCACGTGATC GCGTCGGGGC TCGAGATGCC GGTCGGCGTC GCGTATCGCA GCGGCGCGCT CTTCGTGTCG GCCGTGTCGC GGATTTTACG CCTCGACCGC ATCGACGAGC GGCTCGCCGC GCCGCCGAAG CCCGTCGTCG TCACGAACGC GCTGCCGACC GACCGCCATC ACGGCTGGAA GTTCATCGCG TTCGGCCCGG ACGGCAAGCT CTACGTGCCC ACGGGCGCGC CGTGCAATAT TTGCGTCGCC GACCGCGACC GCTATGCGAT GATCGGCCGG ATGAATGCCG ACGGCAGCGG CTACGAGGTC TACGCGCGAG GCGTGCGCAA CACCGTCGGC TTCGCGTGGC ACCCGGCGAC GCGCGAACTC TGGTTCACCG ACAACGGCCG CGACCTGATG GGCGACGACC GTCCCGACGA CAAGCTCAAT CGCGCGCCGC GCGCGGGCCT CGACTTCGGC TATCCGTTCT GCCACGGCGG CGACGTGCTC GATCCGCAAT TCGGGCGCGG CCACACGTGC TCGAGCTACG CGCCGCCCGT GCTCAAGCTG GGCGCGCACG TCGCCGCGCT CGGCATGCGC TTCTATACGG GCGGCATGTT CCCGCCCGAA TACCGCGACA ATATCTTCAT CGCCGAGCAC GGCTCGTGGA ACCGCAGCCG CAAGGTCGGC TATCGGGTCG TGCGCGTGAT CGCGTCGCCC GACGGCCGCG CCGCGCGCGA GGAAACGTTC GTCCACGGGT GGCTGCGGCC CGACGAAAGC GTATGGGGGC GCCCCGCCGA CGTGCTGCCG CTACCGGACG GCTCGCTCCT CGTGAGCGAC GACTACGCGG GCGCCATCTA CCGAATCACC TACGATGCGA CGCAT
Details for BupsA.17672.a.A1.GE31496
HARVESTED ON: 2/1/2011
SEQUENCED ON: 2/8/2011
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MVATAPAASA ALPIDELRVP PGFRVQVLAD DVPTAREMAW SPRGILYVGS MNGRVHALVV RDGHVREHHV IASGLEMPVG VAYRSGALFV SAVSRILRLD RIDERLAAPP KPVVVTNALP TDRHHGWKFI AFGPDGKLYV PTGAPCNICV ADRDRYAMIG RMNADGSGYE VYARGVRNTV GFAWHPATRE LWFTDNGRDL MGDDRPDDKL NRAPRAGLDF GYPFCHGGDV LDPQFGRGHT CSSYAPPVLK LGAHVAALGM RFYTGGMFPP EYRDNIFIAE HGSWNRSRKV GYRVVRVIAS PDGRAAREET FVHGWLRPDE SVWGRPADVL PLPDGSLLVS DDYAGAIYRI TYDAT
Validated NT Sequence
gcgtcgcatc gtaggtgatt cggtagatgg cgcccgcgta gtcgtcgctc acgaggagcg agccgtccgg tagcggcagc acgtcggcgg ggcgccccca tacgctttcg tcgggccgca gccacccgtg gacgaacgtt tcctcgcgcg cggcgcggcc gtcgggcgac gcgatcacgc gcacgacccg atagccgacc ttgcggctgc ggttccacga gccgtgctcg gcgatgaaga tattgtcgcg gtattcgggc gggaacatgc cgcccgtata gaagcgcatg ccgagcgcgg cgacgtgcgc gcccagcttg agcacgggcg gcgcgtagct cgagcacgtg tggccgcgcc cgaattgcgg atcgagcacg tcgccgccgt ggcagaacgg atagccgaag tcgaggcccg cgcgcggcgc gcgattgagc ttgtcgtcgg gacggtcgtc gcccatcagg tcgcggccgt tgtcggtgaa ccagagttcg cgcgtcgccg ggtgccacgc gaagccgacg gtgttgcgca cgcctcgcgc gtagacctcg tagccgctgc cgtcggcatt catccggccg atcatcgcat agcggtcgcg gtcggcgacg caaatattgc acggcgcgcc cgtgggcacg tagagcttgc cgtccgggcc gaacgcgatg aacttccagc cgtgatggcg gtcggtcggc agcgcgttcg tgacgacgac nggcttcggc ggcgcggcga gccgctcgtc gatgcggtcg aggcgtaaaa tccgcgacac ggccgacacg aagagcgcgc cgctgcgata cgcgacgccg accggcatct cgagccccga cgcgatcacg tggtgttcgc ggacatggcc gtcgcgcacg acgagcgcgt gcactcgtcc gttcatgctg ccgacgtaga ggatgccgcg cggcgaccac gccatctcgc gcgcggtggg cacgtcgtcg gcgagcacct ggacccggaa tccgggcggc acgcggagct cgtcgatcgg caatgcggcc gacgcggcgg gggccgtggc caccatcgaa ccaggaccct gggtctgagc ttccagggta cc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVATAPAAS AALPIDELRV PPGFRVQVLA DDVPTAREMA WSPRGILYVG SMNGRVHALV VRDGHVREHH VIASGLEMPV GVAYRSGALF VSAVSRILRL DRIDERLAAP PKPVVVTNAL PTDRHHGWKF IAFGPDGKLY VPTGAPCNIC VADRDRYAMI GRMNADGSGY EVYARGVRNT VGFAWHPATR ELWFTDNGRD LMGDDRPDDK LNRAPRAGLD FGYPFCHGGD VLDPQFGRGH TCSSYAPPVL KLGAHVAALG MRFYTGGMFP PEYRDNIFIA EHGSWNRSRK VGYRVVRVIA SPDGRAAREE TFVHGWLRPD ESVWGRPADV LPLPDGSLLV SDDYAGAIYR ITYDATH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggccacg gcccccgccg cgtcggccgc attgccgatc gacgagctcc gcgtgccgcc cggattccgg gtccaggtgc tcgccgacga cgtgcccacc gcgcgcgaga tggcgtggtc gccgcgcggc atcctctacg tcggcagcat gaacggacga gtgcacgcgc tcgtcgtgcg cgacggccat gtccgcgaac accacgtgat cgcgtcgggg ctcgagatgc cggtcggcgt cgcgtatcgc agcggcgcgc tcttcgtgtc ggccgtgtcg cggattttac gcctcgaccg catcgacgag cggctcgccg cgccgccgaa gcccgtcgtc gtcacgaacg cgctgccgac cgaccgccat cacggctgga agttcatcgc gttcggcccg gacggcaagc tctacgtgcc cacgggcgcg ccgtgcaata tttgcgtcgc cgaccgcgac cgctatgcga tgatcggccg gatgaatgcc gacggcagcg gctacgaggt ctacgcgcga ggcgtgcgca acaccgtcgg cttcgcgtgg cacccggcga cgcgcgaact ctggttcacc gacaacggcc gcgacctgat gggcgacgac cgtcccgacg acaagctcaa tcgcgcgccg cgcgcgggcc tcgacttcgg ctatccgttc tgccacggcg gcgacgtgct cgatccgcaa ttcgggcgcg gccacacgtg ctcgagctac gcgccgcccg tgctcaagct gggcgcgcac gtcgccgcgc tcggcatgcg cttctatacg ggcggcatgt tcccgcccga ataccgcgac aatatcttca tcgccgagca cggctcgtgg aaccgcagcc gcaaggtcgg ctatcgggtc gtgcgcgtga tcgcgtcgcc cgacggccgc gccgcgcgcg aggaaacgtt cgtccacggg tggctgcggc ccgacgaaag cgtatggggg cgccccgccg acgtgctgcc gctaccggac ggctcgctcc tcgtgagcga cgactacgcg ggcgccatct accgaatcac ctacgatgcg acgcattaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa