SSGCID: Target Page

BupsA.17668.a

Chemotaxis protein cheW

CENTER ID:	BupsA.17668.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17668.a.A1.GE31494	Full length( BupsA.17668.a )		1	201

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.5017
RefSeq:	YP_336123.1
UniProt:	Q3JJY0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFERHAALLL DRPLTDADLA DAARVAAERA PGANAPASKA SAAARDGDAD AVHSALAFRV ADEWLALPIR VLREITDTRA IHPLPHRRNR AVLGIVNVRG MLRVAVSLAE LLSLDARADR AAPRMSFTRM LVVAHRGDPV VFPVDEVEGV LRFASADWMP VPATVARTGA VHSRGVFAWR GKSIGLLDED RLFDSLTRSL R

NT Sequence

GTGTTCGAGC GGCACGCGGC GCTGCTGCTC GATCGCCCGC TGACCGACGC GGATCTCGCC GACGCGGCGC GCGTTGCCGC CGAGCGCGCG CCGGGCGCGA ACGCGCCGGC ATCGAAGGCG TCGGCAGCGG CGCGCGACGG CGATGCCGAC GCCGTGCATT CGGCGCTCGC GTTTCGCGTC GCCGACGAGT GGCTCGCGCT GCCGATTCGC GTGCTGCGCG AGATCACCGA TACGCGCGCG ATTCATCCGC TGCCGCACCG GCGCAACCGC GCGGTGCTCG GCATCGTCAA CGTGCGCGGG ATGCTGCGCG TCGCGGTATC GCTCGCCGAG CTGCTGAGCC TCGATGCGCG CGCGGATCGC GCCGCGCCGC GCATGAGCTT CACGCGGATG CTCGTCGTCG CGCATCGCGG CGATCCGGTC GTGTTTCCGG TCGACGAAGT CGAAGGCGTG CTGCGCTTCG CGTCCGCCGA CTGGATGCCG GTGCCGGCCA CCGTCGCGCG CACGGGCGCC GTGCATTCGC GCGGCGTGTT CGCGTGGCGC GGCAAGTCGA TCGGCCTGCT CGACGAAGAT CGCCTGTTCG ATTCGCTGAC GCGGAGCCTG CGA

Details for BupsA.17668.a.A1.GE31494

HARVESTED ON:	2/1/2011
SEQUENCED ON:	2/8/2011
EXPECTED MW:	24kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	76
PERCENT COVERAGE:	113

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVFERHAAL LLDRPLTDAD LADAARVAAE RAPGANAPAS KASAAARDGD ADAVHSALAF RVADEWLALP IRVLREITDT RAIHPLPHRR NRAVLGIVNV RGMLRVAVSL AELLSLDARA DRAAPRMXFT RMLVVAHRGD PVVFXGRRSR XXAALXVRRL XXXCRXXSRA RAPCIXXXXX RXGXXXXSXA RXXXXXXXXX XXXXXXXXAX XXFCXXXXXX XXXXPXGXXX XXPX

Validated NT Sequence

gtnnnnnnnn nnnngangga gnnataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtgttc gagcggcacg cggcgctgct gctcgatcgc ccgctgaccg acgcggatct cgccgacgcg gcgcgcgttg ccgccgagcg cgcgccgggc gcgaacgcgc cggcatcgaa ggcgtcggca gcggcgcgcg acggcgatgc cgacgccgtg cattcggcgc tcgcgtttcg cgtcgccgac gagtggctcg cgctgccgat tcgcgtgctg cgcgagatca ccgatacgcg cgcgattcat ccgctgccgc accggcgcaa ccgcgcggtg ctcggcatcg tcaacgtgcg cgggatgctg cgcgtcgcgg tatcgctcgc cgagctgctg agcctcgatg cgcgcgcgga tcgcgccgcg ccgcgcatga ncttcacgcg gatgctcgtc gtcgcgcatc gcggcgatcc ggtcgtgttt ncnggtcgac gaagtcgann nngtgctgcg cttcncgtcc gccgactggn ngncngntgc cgncnancgt cgcgcgcacg ggcgccgtgc attcnnnnnn gncnngntcg nnntggnnnn gnnngtcnat cgnnngctcg acnaanatnn ncngntcnan tnnncnnann nngnanncng nnannnnnan nngcnnnnnn nnnnttctgc nnnnnctnnn nnnnnnnnnn nnntnnnnnn ccnaanggna nctnngntnn nnnnccnnnn nnt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVFERHAAL LLDRPLTDAD LADAARVAAE RAPGANAPAS KASAAARDGD ADAVHSALAF RVADEWLALP IRVLREITDT RAIHPLPHRR NRAVLGIVNV RGMLRVAVSL AELLSLDARA DRAAPRMSFT RMLVVAHRGD PVVFPVDEVE GVLRFASADW MPVPATVART GAVHSRGVFA WRGKSIGLLD EDRLFDSLTR SLR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgttcgag cggcacgcgg cgctgctgct cgatcgcccg ctgaccgacg cggatctcgc cgacgcggcg cgcgttgccg ccgagcgcgc gccgggcgcg aacgcgccgg catcgaaggc gtcggcagcg gcgcgcgacg gcgatgccga cgccgtgcat tcggcgctcg cgtttcgcgt cgccgacgag tggctcgcgc tgccgattcg cgtgctgcgc gagatcaccg atacgcgcgc gattcatccg ctgccgcacc ggcgcaaccg cgcggtgctc ggcatcgtca acgtgcgcgg gatgctgcgc gtcgcggtat cgctcgccga gctgctgagc ctcgatgcgc gcgcggatcg cgccgcgccg cgcatgagct tcacgcggat gctcgtcgtc gcgcatcgcg gcgatccggt cgtgtttccg gtcgacgaag tcgaaggcgt gctgcgcttc gcgtccgccg actggatgcc ggtgccggcc accgtcgcgc gcacgggcgc cgtgcattcg cgcggcgtgt tcgcgtggcg cggcaagtcg atcggcctgc tcgacgaaga tcgcctgttc gattcgctga cgcggagcct gcgataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa