SSGCID: Target Page

BupsA.17657.a

Flagellar hook-associated protein 3

CENTER ID:	BupsA.17657.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17657.a.A1.GE31490	Full length( BupsA.17657.a )		1	396

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.474
RefSeq:	YP_331889.2
UniProt:	Q3JX14

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MMNDQQAQIA QLYQQVSSGI SLTTPADNPL AAAQAVQLSA TSATLAQYTQ NQTIVQTALQ TEDTTLTSVN DVLNAAYQAL MHAGDGGLSD SDRAALAAQI QGSRDHLLTL ANTADGAGNY LFAGFQPTTQ PFSNKPGGGV TYAGDYGARA VQIADTRTVS QGDNGANVFM SVPFLGSLPV PAAGASNTGT GTIGAVSITN PSDPTNTHQF TITFGGTAAA PTYTVTDNSV TPPTTTAAQA YSSGQGINLG GQTVAVSGKP AVGDTFTVTP APQAGTDVFA TLDTVIAALK SPVGNSQTAS TALTNTMATA STKLMNTMTN VLTVQASVGG RLQEVKAMQA VTTTNTLQTT NSLSNLTDTN LPAAISQFLQ LQNSLSAAQK AFVQMQNLSL FQYLNP

NT Sequence

ATGATGAACG ACCAGCAGGC GCAGATCGCG CAGCTCTACC AGCAGGTATC GAGCGGCATC AGCCTGACGA CGCCCGCCGA CAATCCGCTC GCGGCCGCGC AGGCGGTGCA GCTGTCGGCG ACCTCGGCGA CGCTCGCGCA GTACACGCAG AACCAGACGA TCGTGCAGAC CGCGCTGCAG ACCGAGGACA CGACGCTCAC GAGCGTCAAC GACGTGCTCA ACGCCGCGTA CCAGGCGCTG ATGCACGCGG GCGACGGCGG GCTGTCGGAC AGCGACCGCG CGGCGCTTGC CGCGCAGATC CAGGGCTCGC GCGACCATCT GCTCACGCTC GCGAACACGG CGGACGGCGC GGGCAACTAT CTGTTCGCGG GCTTCCAGCC GACGACGCAG CCGTTCTCGA ACAAGCCGGG CGGCGGCGTC ACGTATGCGG GCGATTACGG CGCGCGCGCC GTGCAGATCG CCGATACGCG CACGGTTTCG CAGGGCGACA ACGGCGCGAA CGTGTTCATG TCGGTGCCGT TCCTCGGCAG CCTGCCCGTG CCCGCCGCGG GCGCGAGCAA CACGGGCACG GGCACGATCG GCGCGGTCAG CATCACGAAT CCGTCCGATC CGACCAACAC GCACCAGTTC ACGATCACGT TCGGCGGCAC GGCCGCGGCG CCGACTTACA CGGTGACCGA CAACTCGGTC ACGCCGCCGA CGACGACCGC GGCGCAGGCG TACTCGTCCG GCCAGGGCAT CAATCTCGGC GGCCAGACGG TCGCGGTGTC GGGCAAGCCG GCCGTGGGCG ACACGTTCAC GGTGACGCCC GCGCCGCAGG CGGGCACCGA CGTGTTCGCG ACGCTCGACA CGGTGATCGC CGCGCTGAAG TCGCCCGTCG GCAATAGCCA GACCGCGTCG ACCGCGCTGA CGAACACGAT GGCCACCGCG TCGACGAAGC TGATGAACAC GATGACGAAC GTGCTGACCG TGCAGGCGTC GGTCGGCGGC CGCCTGCAGG AAGTGAAGGC GATGCAGGCG GTGACGACGA CGAACACGCT GCAGACGACG AACAGCCTGT CGAACCTGAC CGACACGAAC CTGCCCGCCG CGATCAGCCA GTTCCTGCAG CTGCAGAATT CGCTGAGCGC CGCGCAGAAG GCGTTCGTGC AGATGCAGAA CCTGTCGCTG TTCCAGTATC TGAACCCG

Details for BupsA.17657.a.A1.GE31490

HARVESTED ON:	2/1/2011
SEQUENCED ON:	2/8/2011
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

Validated NT Sequence

tcagatactg gaacagcgac aggttctgca tctgcacgaa cgccttctgc gcggcgctca gcgaattctg cagctgcagg aactggctga tcgcggcggg caggttcgtg tcggtcaggt tcgacaggct gttcgtcgtc tgcagcgtgt tcgtcgtcgt caccgcctgc atcgccttca cttcctgcag gcggccgccg accgacgcct gcacggtcag cacgttcgtc atcgtgttca tcagcttcgt cgacgcggtg gccatcgtgt tcgtcagcgc ggtcgacgcg gtctggctat tgccgacggg cgacttcagc gcggcgatca ccgtgtcgag cgtcgcgaac acgtcggtgc ccgcctgcgg cgcgggcgtc accgtgaacg tgtcgcccac ggccggcttg cccgacaccg cgaccgtctg gccgccgaga ttgatgccct ggccggacga gtacgcctgc gccgcggtcg tcgtcggcgg cgtgaccgag ttgtcggtca ccgtgtaagt cggcgccgcg gccgtgccgc cgaacgtgat cgtgaactgg tgcgtgttgg tcggatcgga cggattcgtg atgctgaccg cgccgatcgt gcccgtgccc gtgttgctcg cgcccgcggc gggcacgggc aggctgccga ggaacggcac cgacatgaac acgttcgcgc cgttgtcgcc ctgcgaaacc gtgcgcgtat cggcgatctg cacggcgcgc gcgccgtaat cgcccgcata cgtgacgccg ccgcccggct tgttcgagaa cggctgcgtc gtcggctgga agcccgcgaa cagatagttg cccgcgccgt ccgccgtgtt cgcgagcgtg agcagatggt cgcgcgagcc ctggatctgc gcggcaagcg ccgcgcggtc gctgtccgac agcccgccgt cgcccgcgtg catcagcgcc tggtacgcgg cgttgagcac gtcgttgacg ctcgtgagcg tcgtgtcctc ggtctgcagc gcggtctgca cgatcgtctg gttctgcgtg tactgcgcga gcgtcgccga ggtcgccgac agctgcaccg cctgcgcggc cgcgagcgga ttgtcggcgg gcgtcgtcag gctgatgccg ctcgatacct gctggtagag ctgcgcgatc tgcgcctgct ggtcgttcat catcgaacca ggacc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMMNDQQAQI AQLYQQVSSG ISLTTPADNP LAAAQAVQLS ATSATLAQYT QNQTIVQTAL QTEDTTLTSV NDVLNAAYQA LMHAGDGGLS DSDRAALAAQ IQGSRDHLLT LANTADGAGN YLFAGFQPTT QPFSNKPGGG VTYAGDYGAR AVQIADTRTV SQGDNGANVF MSVPFLGSLP VPAAGASNTG TGTIGAVSIT NPSDPTNTHQ FTITFGGTAA APTYTVTDNS VTPPTTTAAQ AYSSGQGINL GGQTVAVSGK PAVGDTFTVT PAPQAGTDVF ATLDTVIAAL KSPVGNSQTA STALTNTMAT ASTKLMNTMT NVLTVQASVG GRLQEVKAMQ AVTTTNTLQT TNSLSNLTDT NLPAAISQFL QLQNSLSAAQ KAFVQMQNLS LFQYLNP

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatgaacgac cagcaggcgc agatcgcgca gctctaccag caggtatcga gcggcatcag cctgacgacg cccgccgaca atccgctcgc ggccgcgcag gcggtgcagc tgtcggcgac ctcggcgacg ctcgcgcagt acacgcagaa ccagacgatc gtgcagaccg cgctgcagac cgaggacacg acgctcacga gcgtcaacga cgtgctcaac gccgcgtacc aggcgctgat gcacgcgggc gacggcgggc tgtcggacag cgaccgcgcg gcgcttgccg cgcagatcca gggctcgcgc gaccatctgc tcacgctcgc gaacacggcg gacggcgcgg gcaactatct gttcgcgggc ttccagccga cgacgcagcc gttctcgaac aagccgggcg gcggcgtcac gtatgcgggc gattacggcg cgcgcgccgt gcagatcgcc gatacgcgca cggtttcgca gggcgacaac ggcgcgaacg tgttcatgtc ggtgccgttc ctcggcagcc tgcccgtgcc cgccgcgggc gcgagcaaca cgggcacggg cacgatcggc gcggtcagca tcacgaatcc gtccgatccg accaacacgc accagttcac gatcacgttc ggcggcacgg ccgcggcgcc gacttacacg gtgaccgaca actcggtcac gccgccgacg acgaccgcgg cgcaggcgta ctcgtccggc cagggcatca atctcggcgg ccagacggtc gcggtgtcgg gcaagccggc cgtgggcgac acgttcacgg tgacgcccgc gccgcaggcg ggcaccgacg tgttcgcgac gctcgacacg gtgatcgccg cgctgaagtc gcccgtcggc aatagccaga ccgcgtcgac cgcgctgacg aacacgatgg ccaccgcgtc gacgaagctg atgaacacga tgacgaacgt gctgaccgtg caggcgtcgg tcggcggccg cctgcaggaa gtgaaggcga tgcaggcggt gacgacgacg aacacgctgc agacgacgaa cagcctgtcg aacctgaccg acacgaacct gcccgccgcg atcagccagt tcctgcagct gcagaattcg ctgagcgccg cgcagaaggc gttcgtgcag atgcagaacc tgtcgctgtt ccagtatctg aacccgtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa