BupsA.17647.a

Putative phage-related integrase

CENTER ID: BupsA.17647.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17647.a.A1.GE31484 Full length( BupsA.17647.a ) 1 412
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.978
RefSeq: YP_332380.1
UniProt: Q3JVM3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MALAFNLELA MKTPDDKPKA ADFNLDAESL KRLDVIFPDG TQVKDINTDD DVRRAKELFG DRFAAAVPAP PSFDLPAFSP SVAAAIAAQK AVQETKRKAK PFADVAKLYK KEKALDNTAR TITAKERAFA DFEKRIGSRP IDAYLIDDAV AYKNALIDEG GSASRINAKL SFLRDLFAYA LANGLRAEAN PFENVKVSSK SKLEQQKRSY KPFSADDIAT IFDPTAYPAR MDKPAYRWFP FLALYSGARL EELASLSLSQ IQREGDVWFF DIQKAKNANS QRRIPLHRVV VESGFLAYAE GLRERGATQL FPELKPGANG YGKNITRRFA DYLDERKITD DRKVFHSFRH TFIGRMTELN VHPAMLMALV GHYDQAKVDF SSPHFANYQH VKPLAELKAT LDRFDIDLPM AF
NT Sequence
TTGGCGCTCG CGTTCAATCT CGAACTCGCC ATGAAGACGC CGGACGACAA GCCGAAGGCC GCAGACTTCA ACCTCGACGC CGAATCGCTC AAACGGCTCG ACGTGATCTT CCCGGACGGC ACCCAGGTCA AGGACATCAA CACCGACGAC GACGTCCGCC GCGCCAAAGA GCTGTTCGGC GACCGCTTCG CCGCCGCCGT CCCGGCCCCG CCCTCGTTCG ACTTGCCCGC CTTCTCGCCG TCCGTCGCCG CCGCGATCGC CGCCCAGAAA GCCGTGCAGG AAACGAAGCG CAAGGCCAAG CCGTTCGCCG ACGTGGCGAA GCTCTACAAG AAGGAAAAGG CTCTCGACAA CACGGCGCGC ACGATCACGG CCAAAGAGCG GGCGTTCGCG GACTTCGAGA AGCGAATCGG CTCGCGGCCG ATCGACGCCT ACTTGATCGA CGACGCCGTT GCCTACAAGA ACGCTTTGAT CGACGAAGGC GGCAGCGCGA GCCGGATCAA CGCAAAGCTG TCGTTTCTGC GCGACCTGTT CGCCTACGCC CTGGCGAACG GCCTGCGGGC TGAGGCGAAC CCGTTCGAAA ACGTCAAAGT CTCGTCCAAG TCGAAGCTCG AACAGCAAAA GCGCTCCTAC AAACCGTTCA GCGCGGACGA CATCGCGACG ATCTTCGACC CGACGGCCTA TCCCGCGCGC ATGGACAAGC CGGCTTACCG TTGGTTTCCC TTCCTCGCGC TCTACTCCGG CGCGCGGCTC GAAGAGCTTG CCAGCCTGTC GCTGTCGCAA ATCCAGCGCG AGGGCGACGT TTGGTTCTTC GACATCCAGA AGGCGAAGAA CGCGAACTCC CAGCGGCGCA TCCCCCTGCA TCGCGTCGTA GTCGAGTCGG GTTTCCTCGC CTACGCGGAG GGTCTTCGCG AGCGTGGCGC GACGCAGCTA TTCCCGGAGT TGAAGCCGGG CGCGAACGGC TACGGAAAAA ACATCACGCG GCGCTTCGCG GACTATCTCG ACGAGCGCAA GATAACGGAC GACCGCAAGG TGTTCCACTC GTTCCGGCAC ACGTTCATCG GCCGCATGAC CGAGCTGAAC GTCCACCCGG CTATGCTCAT GGCGCTCGTC GGGCATTACG ATCAAGCGAA AGTCGATTTC AGCTCGCCGC ACTTCGCGAA CTACCAGCAC GTCAAGCCTC TCGCGGAGCT TAAAGCGACT CTCGACCGCT TTGACATTGA TTTGCCGATG GCTTTT
Details for BupsA.17647.a.A1.GE31484
HARVESTED ON: 2/1/2011
SEQUENCED ON: 2/8/2011
EXPECTED MW: 48kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 16
PERCENT COVERAGE: 53
Validated AA Sequence
MXXXLLXXXX XPSYGYPGSS DPGSWFDVGA RVQSRTRHED AGRQAEGRRL QPRRRIAQTA RRDLPGRHPG QGHQHRRRRP PRQRAVRRPL RRRRPGPALV RLARLLAVRR RRDRRPESRA GNEAQGQAVR RRGEALQEGK GSRQXGAHDH GXRAGVXGLX XXNRXAAXRR LLDRXXRXLX XXFDRXXXXX SXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX X
Validated NT Sequence
gcggnncagc catgttnnnn nnnctccttg nnnnnnnnnn nncaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgttggcgc tcgcgttcaa tctcgaactc gccatgaaga cgccggacga caagccgaag gccgcagact tcaacctcga cgccgaatcg ctcaaacggc tcgacgtgat cttcccggac ggcacccagg tcaaggacat caacaccgac gacgacgtcc gccgcgccaa agagctgttc ggcgaccgct tcgccgccgc cgtcccggcc ccgccctcgt tcgacttgcc cgccttctcg ccgtccgtcg ccgccgcgat cgccgcccag aaagccgtgc aggaaacgaa gcgcaaggcc aagccgttcg ccgacgtggc gaagctctac aagaaggaaa aggctctcga caacncggcg cgcacgatca cggccnaaga gcgggcgttc nnggacttcn nnnancgaat cggcncgcgg ccgancgacg cctacttgat cgncnannnc gttncctann anaangcttt gatcgannaa nnnngnnnnn nagccnnnnn cannncnang nngnnntttn nnnnnnnann nnnncncnnn nnnccngnnn nnnnnnnnng gnctnnnnnn nnancngnnn nnanncnnnt nnnnnnnnnn nnnnnnnnnn ncnnaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLALAFNLE LAMKTPDDKP KAADFNLDAE SLKRLDVIFP DGTQVKDINT DDDVRRAKEL FGDRFAAAVP APPSFDLPAF SPSVAAAIAA QKAVQETKRK AKPFADVAKL YKKEKALDNT ARTITAKERA FADFEKRIGS RPIDAYLIDD AVAYKNALID EGGSASRINA KLSFLRDLFA YALANGLRAE ANPFENVKVS SKSKLEQQKR SYKPFSADDI ATIFDPTAYP ARMDKPAYRW FPFLALYSGA RLEELASLSL SQIQREGDVW FFDIQKAKNA NSQRRIPLHR VVVESGFLAY AEGLRERGAT QLFPELKPGA NGYGKNITRR FADYLDERKI TDDRKVFHSF RHTFIGRMTE LNVHPAMLMA LVGHYDQAKV DFSSPHFANY QHVKPLAELK ATLDRFDIDL PMAF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttggcgctc gcgttcaatc tcgaactcgc catgaagacg ccggacgaca agccgaaggc cgcagacttc aacctcgacg ccgaatcgct caaacggctc gacgtgatct tcccggacgg cacccaggtc aaggacatca acaccgacga cgacgtccgc cgcgccaaag agctgttcgg cgaccgcttc gccgccgccg tcccggcccc gccctcgttc gacttgcccg ccttctcgcc gtccgtcgcc gccgcgatcg ccgcccagaa agccgtgcag gaaacgaagc gcaaggccaa gccgttcgcc gacgtggcga agctctacaa gaaggaaaag gctctcgaca acacggcgcg cacgatcacg gccaaagagc gggcgttcgc ggacttcgag aagcgaatcg gctcgcggcc gatcgacgcc tacttgatcg acgacgccgt tgcctacaag aacgctttga tcgacgaagg cggcagcgcg agccggatca acgcaaagct gtcgtttctg cgcgacctgt tcgcctacgc cctggcgaac ggcctgcggg ctgaggcgaa cccgttcgaa aacgtcaaag tctcgtccaa gtcgaagctc gaacagcaaa agcgctccta caaaccgttc agcgcggacg acatcgcgac gatcttcgac ccgacggcct atcccgcgcg catggacaag ccggcttacc gttggtttcc cttcctcgcg ctctactccg gcgcgcggct cgaagagctt gccagcctgt cgctgtcgca aatccagcgc gagggcgacg tttggttctt cgacatccag aaggcgaaga acgcgaactc ccagcggcgc atccccctgc atcgcgtcgt agtcgagtcg ggtttcctcg cctacgcgga gggtcttcgc gagcgtggcg cgacgcagct attcccggag ttgaagccgg gcgcgaacgg ctacggaaaa aacatcacgc ggcgcttcgc ggactatctc gacgagcgca agataacgga cgaccgcaag gtgttccact cgttccggca cacgttcatc ggccgcatga ccgagctgaa cgtccacccg gctatgctca tggcgctcgt cgggcattac gatcaagcga aagtcgattt cagctcgccg cacttcgcga actaccagca cgtcaagcct ctcgcggagc ttaaagcgac tctcgaccgc tttgacattg atttgccgat ggctttttaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa