SSGCID: Target Page

BupsA.17645.a

RNA polymerase sigma-70 factor, ECF subfamily

CENTER ID:	BupsA.17645.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17645.a.A1.GE31483	Full length( BupsA.17645.a )		1	231

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.5682
RefSeq:	YP_336724.1
UniProt:	Q3JI79

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTTEPRSGDM QPSRVDDRAL AARVAGGDHA AFEQLMRRYN RRLYRLARAT LRDDAQAEDV LQEAYLAAFR TIGRFRGDAS LSTWLSRLVL NACFTRQRRG ARRDNIAPMV NTSSIAGFER TTMDADLTSS PDHALMRTEL RALLERKLDA LPEAFRLVFV LRCVEELSVE ETAQCLGIPE ATVRSRHFRA KRLLRESLAR EIDSAGRDVF SFAGERCDRI VRGVLARLIS P

NT Sequence

ATGACGACCG AGCCGCGATC GGGCGACATG CAGCCTTCGC GCGTGGACGA CCGCGCGCTG GCCGCGCGGG TTGCCGGCGG CGATCATGCG GCATTCGAGC AATTGATGCG CCGCTACAAC CGGCGCCTCT ACCGGCTTGC GCGCGCGACG TTGCGCGACG ACGCGCAAGC CGAAGACGTG CTTCAGGAGG CCTATCTGGC CGCATTTCGC ACCATCGGGC GCTTTCGCGG CGACGCATCG CTGTCCACCT GGCTGTCGCG CCTCGTGCTC AACGCGTGCT TTACGCGCCA GCGCCGGGGC GCCCGGCGCG ACAACATCGC CCCGATGGTG AATACATCGT CCATCGCCGG CTTCGAGCGC ACTACCATGG ATGCGGACCT AACCTCCTCG CCCGATCATG CACTCATGCG CACGGAGCTG CGTGCATTGC TCGAGCGCAA GCTCGATGCG TTGCCCGAGG CTTTTCGCCT CGTGTTCGTC CTGCGTTGCG TGGAGGAGTT GAGCGTCGAG GAGACCGCGC AGTGTCTCGG CATACCGGAG GCCACCGTGC GATCGCGCCA TTTTCGGGCG AAGCGCTTAC TGCGCGAATC GCTCGCGCGG GAAATCGATT CGGCCGGGCG CGACGTGTTC TCGTTCGCCG GCGAGCGCTG CGACCGCATC GTCCGCGGCG TACTGGCGAG GCTGATTTCG CCC

Details for BupsA.17645.a.A1.GE31483

HARVESTED ON:	2/1/2011
SEQUENCED ON:	2/8/2011
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMTTEPRSGD MQPSRVDDRA LAARVAGGDH AAFEQLMRRY NRRLYRLARA TLRDDAQAED VLQEAYLAAF RTIGRFRGDA SLSTWLSRLV LNACFTRQRR GARRDNIAPM VNTSSIAGFE RTTMDADLTS SPDHALMRTE LRALLERKLD ALPEAFRLVF VLRCVEELSV EETAQCLGIP EATVRSRHFR AKRLLRESLA REIDSAGRDV FSFAGERCDR IVRGVLARLI SP

Validated NT Sequence

ttattagggc gaaatcagcc tcgccagtac gccgcggacg atgcggtcgc agcgctcgcc ggcgaacgag aacacgtcgc gcccggccga atcgatttcc cgcgcgagcg attcgcgcag taagcgcttc gcccgaaaat ggcgcgatcg cacggtggcc tccggtatgc cgagacactg cgcggtctcc tcgacgctca actcctccac gcaacgcagg acgaacacga ggcgaaaagc ctcgggcaac gcatcgagct tgcgctcgag caatgcacgc agctccgtgc gcatgagtgc atgatcgggc gaggaggtta ggtccgcatc catggtagtg cgctcgaagc cggcgatgga cgatgtattc accatcgggg cgatgttgtc gcgccgggcg ccccggcgct ggcgcgtaaa gcacgcgttg agcacgaggc gcgacagcca ggtggacagc gatgcgtcgc cgcgaaagcg cccgatggtg cgaaatgcgg ccagataggc ctcctgaagc acgtcttcgg cttgcgcgtc gtcgcgcaac gtcgcgcgcg caagccggta gaggcgccgg ttgtagcggc gcatcaattg ctcgaatgcc gcatgatcgc cgccggcaac ccgcgcggcc agcgcgcggt cgtccacgcg cgaaggctgc atgtcgcccg atcgcggctc ggtcgtcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgaccgag ccgcgatcgg gcgacatgca gccttcgcgc gtggacgacc gcgcgctggc cgcgcgggtt gccggcggcg atcatgcggc attcgagcaa ttgatgcgcc gctacaaccg gcgcctctac cggcttgcgc gcgcgacgtt gcgcgacgac gcgcaagccg aagacgtgct tcaggaggcc tatctggccg catttcgcac catcgggcgc tttcgcggcg acgcatcgct gtccacctgg ctgtcgcgcc tcgtgctcaa cgcgtgcttt acgcgccagc gccggggcgc ccggcgcgac aacatcgccc cgatggtgaa tacatcgtcc atcgccggct tcgagcgcac taccatggat gcggacctaa cctcctcgcc cgatcatgca ctcatgcgca cggagctgcg tgcattgctc gagcgcaagc tcgatgcgtt gcccgaggct tttcgcctcg tgttcgtcct gcgttgcgtg gaggagttga gcgtcgagga gaccgcgcag tgtctcggca taccggaggc caccgtgcga tcgcgccatt ttcgggcgaa gcgcttactg cgcgaatcgc tcgcgcggga aatcgattcg gccgggcgcg acgtgttctc gttcgccggc gagcgctgcg accgcatcgt ccgcggcgta ctggcgaggc tgatttcgcc ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa