BupsA.17639.a

YD repeat protein

CENTER ID: BupsA.17639.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17639.a.A1.GE31479 Full length( BupsA.17639.a ) 1 425
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.5282
RefSeq: YP_336348.1
UniProt: Q3JJA5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGRASAMIDP AGRTTAWEYD AYGSLLVQTL PDGSAVRTEF DLDHRPVCMT LIGGRQWGYE WNTFGNLLAQ SDPSGAISRY TYDEYGQLVE HTGPRGASTR FDYHPDGNLA AQIDALGHRT QYRYDARGYL GEAIDALGQQ SQYEYDRNGH LTRAIEPGGR EIHCAYDADG NLSRHRDPMG HVTQVEYSAL GQVSRRLAPD GTTVEYRYDS HITSAGFRTR PIGRLPMFAC QTCRRYFSRT AAPPLGEKHL KKLDLFVSLL SHPISCVDAG EQMGSLSTDI GKRVTAWRAW LLELDPSGKW ERRVRLSHRP PHCPNCGSHQ TRFDECSNGA FPRFKCANCG TKFTRRRGTP FVNAKMSSPE RMRLVIRRLS LPLLVMQVAD LVGTSHGMVR KWHSMFTDFA DRLEPSGSLS ARIRLRSNSA NAPNK
NT Sequence
GTGGGGCGTG CGAGCGCGAT GATCGATCCG GCGGGGCGGA CGACGGCTTG GGAATATGAC GCGTATGGCA GTTTGCTTGT GCAGACGTTG CCGGATGGCA GCGCAGTCAG AACGGAATTT GACCTCGATC ACCGACCGGT CTGCATGACG TTGATAGGCG GCCGGCAGTG GGGCTACGAG TGGAATACGT TCGGTAATCT GCTCGCGCAG AGCGATCCAT CGGGGGCGAT ATCTCGCTAT ACCTATGACG AGTACGGCCA GCTTGTTGAG CATACTGGGC CGCGTGGTGC GAGCACACGG TTCGATTATC ACCCGGACGG CAATCTCGCG GCGCAGATCG ATGCGTTGGG GCATCGCACG CAGTATCGGT ACGATGCGCG CGGCTACCTC GGCGAAGCAA TCGATGCGCT CGGACAGCAA AGCCAATACG AGTACGACCG CAACGGCCAT CTGACGCGCG CAATCGAGCC GGGCGGGCGT GAGATTCACT GTGCGTACGA CGCCGATGGA AATCTGTCTC GCCATCGTGA CCCCATGGGC CACGTGACGC AGGTGGAGTA CTCGGCGCTC GGACAGGTCA GCAGACGGCT CGCGCCCGAC GGCACCACCG TTGAATACCG CTACGACAGC CACATTACCA GCGCGGGATT CCGAACGCGG CCCATCGGTC GGCTGCCGAT GTTCGCGTGC CAGACTTGCC GGCGCTACTT CAGTCGCACG GCCGCCCCCC CACTCGGCGA GAAACATCTC AAGAAACTCG ATCTATTCGT GTCCTTGCTG TCGCATCCGA TCTCGTGCGT TGATGCGGGC GAACAGATGG GCAGCCTATC GACCGACATC GGAAAACGCG TGACGGCCTG GCGCGCGTGG CTGTTGGAGC TCGACCCGAG CGGCAAGTGG GAGCGCCGCG TGAGGCTCAG CCATCGACCT CCGCATTGCC CGAACTGCGG CAGTCACCAG ACGCGTTTCG ATGAATGCTC GAACGGCGCC TTCCCACGGT TCAAATGCGC GAATTGCGGG ACCAAATTCA CCCGACGCCG CGGCACGCCG TTCGTCAATG CGAAGATGAG TTCGCCCGAG CGCATGCGCC TGGTCATTCG GCGCCTGTCG CTGCCGTTGT TGGTCATGCA GGTGGCGGAC CTTGTCGGCA CGAGCCATGG GATGGTCCGG AAATGGCACA GCATGTTCAC CGATTTTGCG GATCGGCTCG AACCGAGTGG CAGTCTTTCA GCGCGGATCA GGTTGCGCTC GAACTCTGCC AATGCGCCGA ACAAA
Details for BupsA.17639.a.A1.GE31479
HARVESTED ON: 2/1/2011
SEQUENCED ON: 2/8/2011
EXPECTED MW: 50kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 22
PERCENT COVERAGE: 32
Validated AA Sequence
MTLIGGRXWG YXWNTFGNLL XXSDPSGAIS XXXXDXXXXL XXXXGXXXXA XXXXXXXTXX XXXXXXXXXX XXXXXXXXXX XXXXXQXXXX XXXXXXXXXX XXXXGXXSXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXX
Validated NT Sequence
cnnnnnnnnn cnnnnnnnnn tgcggnncag ccatgtttan ntnnctcctt ngnnnnannn nnnnnnnacn atnancnnnn nnccctggaa gctcagaccc ngggtcctgg ttcnatggtg gggcgtgcna gcgcgatgat cgatccggcg gggcggacna cggcttggga atatgacgcg natggnantt tgcttgtgan acgttgccgg atggcagcgc agtcagaacg gaatttgacc tcnatcaccg accggtctgc atgacgttga taggcggncg gnngtggggc tacnagtgga atacnttcgg taatctgctc ncnnagagcg atccntcngg ggcgatatct nnntntancn ntgacnagtn cngncngctt gntgancnnn ntgggncggn ggngnnngcn cnnngnnnna ntnnnnncnn gacggnncnn nnnnnnnnnn nnnnggnntt nnnnnnnnnn nnnnnncncn gntncnncnn cnaannnnnn nntncnnnnn gannncaann nnnnntnngn ncnancnnnn nngnnnnnng gnnnnnnnnn nnnngnnnng nnggntgnnn ttccnngnnn nnnnnnnnnc nannnnnnnn ngnnncnnnn nnnnnggnnn nnnnnnnnng nngnnaannn nnnnnnnnna nnnnnannnn nnnnnccnnt nnnnnnnnng nnnnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVGRASAMI DPAGRTTAWE YDAYGSLLVQ TLPDGSAVRT EFDLDHRPVC MTLIGGRQWG YEWNTFGNLL AQSDPSGAIS RYTYDEYGQL VEHTGPRGAS TRFDYHPDGN LAAQIDALGH RTQYRYDARG YLGEAIDALG QQSQYEYDRN GHLTRAIEPG GREIHCAYDA DGNLSRHRDP MGHVTQVEYS ALGQVSRRLA PDGTTVEYRY DSHITSAGFR TRPIGRLPMF ACQTCRRYFS RTAAPPLGEK HLKKLDLFVS LLSHPISCVD AGEQMGSLST DIGKRVTAWR AWLLELDPSG KWERRVRLSH RPPHCPNCGS HQTRFDECSN GAFPRFKCAN CGTKFTRRRG TPFVNAKMSS PERMRLVIRR LSLPLLVMQV ADLVGTSHGM VRKWHSMFTD FADRLEPSGS LSARIRLRSN SANAPNK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggggcgt gcgagcgcga tgatcgatcc ggcggggcgg acgacggctt gggaatatga cgcgtatggc agtttgcttg tgcagacgtt gccggatggc agcgcagtca gaacggaatt tgacctcgat caccgaccgg tctgcatgac gttgataggc ggccggcagt ggggctacga gtggaatacg ttcggtaatc tgctcgcgca gagcgatcca tcgggggcga tatctcgcta tacctatgac gagtacggcc agcttgttga gcatactggg ccgcgtggtg cgagcacacg gttcgattat cacccggacg gcaatctcgc ggcgcagatc gatgcgttgg ggcatcgcac gcagtatcgg tacgatgcgc gcggctacct cggcgaagca atcgatgcgc tcggacagca aagccaatac gagtacgacc gcaacggcca tctgacgcgc gcaatcgagc cgggcgggcg tgagattcac tgtgcgtacg acgccgatgg aaatctgtct cgccatcgtg accccatggg ccacgtgacg caggtggagt actcggcgct cggacaggtc agcagacggc tcgcgcccga cggcaccacc gttgaatacc gctacgacag ccacattacc agcgcgggat tccgaacgcg gcccatcggt cggctgccga tgttcgcgtg ccagacttgc cggcgctact tcagtcgcac ggccgccccc ccactcggcg agaaacatct caagaaactc gatctattcg tgtccttgct gtcgcatccg atctcgtgcg ttgatgcggg cgaacagatg ggcagcctat cgaccgacat cggaaaacgc gtgacggcct ggcgcgcgtg gctgttggag ctcgacccga gcggcaagtg ggagcgccgc gtgaggctca gccatcgacc tccgcattgc ccgaactgcg gcagtcacca gacgcgtttc gatgaatgct cgaacggcgc cttcccacgg ttcaaatgcg cgaattgcgg gaccaaattc acccgacgcc gcggcacgcc gttcgtcaat gcgaagatga gttcgcccga gcgcatgcgc ctggtcattc ggcgcctgtc gctgccgttg ttggtcatgc aggtggcgga ccttgtcggc acgagccatg ggatggtccg gaaatggcac agcatgttca ccgattttgc ggatcggctc gaaccgagtg gcagtctttc agcgcggatc aggttgcgct cgaactctgc caatgcgccg aacaaataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa