BupsA.17630.a

Pyrophosphatase, MutT/nudix family

CENTER ID: BupsA.17630.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17630.a.A1.GE31475 Full length( BupsA.17630.a ) 1 196
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17630.a.A1.PW31373 Full length( BupsA.17630.a ) 1 196

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.1497
RefSeq: YP_332860.1
UniProt: Q3JU93

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MADLPNHDAT LTETCIESES VYDGAFLKVK RDTVRLPDGK HATREYVTHP GAVMVIPLFD NGRVLMESQY RYPIGKVMAE FPAGKLDPNE GALACAVREL REETGYTARE YVFLTRIHPI ISYSTEFIDI YLARGLTAGE RKLDDGEFLE TFTATLPDLL EWVRTGRISD VKTIIGTMWL EKAVSGAWPL GDVIRP
NT Sequence
ATGGCCGATT TGCCGAATCA CGACGCCACC CTCACCGAAA CTTGCATCGA AAGCGAATCC GTCTACGACG GCGCGTTCCT GAAGGTCAAG CGCGACACCG TTCGTCTGCC GGACGGCAAG CATGCGACGC GCGAATACGT GACGCATCCG GGCGCGGTGA TGGTCATCCC GCTGTTCGAC AACGGCCGCG TGCTGATGGA AAGCCAGTAC CGCTATCCGA TCGGCAAGGT GATGGCCGAG TTTCCGGCGG GCAAGCTCGA TCCGAACGAA GGCGCGCTCG CCTGCGCGGT GCGCGAACTG CGTGAGGAAA CCGGCTACAC CGCGCGCGAA TACGTGTTCC TCACGCGCAT TCATCCGATC ATCTCGTATT CGACCGAATT CATCGATATC TATCTCGCGC GCGGCCTGAC GGCGGGCGAG CGCAAGCTCG ACGACGGCGA GTTCCTCGAG ACGTTCACGG CGACGCTGCC GGACCTGCTC GAGTGGGTGC GCACCGGCCG GATCAGCGAC GTCAAGACGA TCATCGGCAC GATGTGGCTC GAGAAGGCGG TATCGGGCGC ATGGCCGCTC GGTGACGTGA TCCGGCCG
Details for BupsA.17630.a.A1.GE31475
HARVESTED ON: 2/1/2011
SEQUENCED ON: 2/8/2011
EXPECTED MW: 24kDa
OBSERVED MW: 24kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMADLPNHDA TLTETCIESE SVYDGAFLKV KRDTVRLPDG KHATREYVTH PGAVMVIPLF DNGRVLMESQ YRYPIGKVMA EFPAGKLDPN EGALACAVRE LREETGYTAR EYVFLTRIHP IISYSTEFID IYLARGLTAG ERKLDDGEFL ETFTATLPDL LEWVRTGRIS DVKTIIGTMW LEKAVSGAWP LGDVIRP
Validated NT Sequence
gtgctgttta ttacggccgg atcacgtcac cgagcggcca tgcgcccgat accgccttct cgagccacat cgtgccgatg atcgtcttga cgtcgctgat ccggccggtg cgcacccact cgagcaggtc cggcagcgtc gccgtgaacg tctcgaggaa ctcgccgtcg tcgagcttgc gctcgcccgc cgtcaggccg cgcgcgagat agatatcgat gaattcggtc gaatacgaga tgatcggatg aatgcgcgtg aggaacacgt attcgcgcgc ggtgtagccg gtttcctcac gcagttcgcg caccgcgcag gcgagcgcgc cttcgttcgg atcgagcttg cccgccggaa actcggccat caccttgccg atcggatagc ggtactggct ttccatcagc acgcggccgt tgtcgaacag cgggatgacc atcaccgcgc ccggatgcgt cacgtattcg cgcgtcgcat gcttgccgtc cggcagacga acggtgtcgc gcttgacctt caggaacgcg ccgtcgtaga cggattcgct ttcgatgcaa gtttcggtga gggtggcgtc gtgattcggc aaatcggcca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMADLPNHDA TLTETCIESE SVYDGAFLKV KRDTVRLPDG KHATREYVTH PGAVMVIPLF DNGRVLMESQ YRYPIGKVMA EFPAGKLDPN EGALACAVRE LREETGYTAR EYVFLTRIHP IISYSTEFID IYLARGLTAG ERKLDDGEFL ETFTATLPDL LEWVRTGRIS DVKTIIGTMW LEKAVSGAWP LGDVIRP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgatttg ccgaatcacg acgccaccct caccgaaact tgcatcgaaa gcgaatccgt ctacgacggc gcgttcctga aggtcaagcg cgacaccgtt cgtctgccgg acggcaagca tgcgacgcgc gaatacgtga cgcatccggg cgcggtgatg gtcatcccgc tgttcgacaa cggccgcgtg ctgatggaaa gccagtaccg ctatccgatc ggcaaggtga tggccgagtt tccggcgggc aagctcgatc cgaacgaagg cgcgctcgcc tgcgcggtgc gcgaactgcg tgaggaaacc ggctacaccg cgcgcgaata cgtgttcctc acgcgcattc atccgatcat ctcgtattcg accgaattca tcgatatcta tctcgcgcgc ggcctgacgg cgggcgagcg caagctcgac gacggcgagt tcctcgagac gttcacggcg acgctgccgg acctgctcga gtgggtgcgc accggccgga tcagcgacgt caagacgatc atcggcacga tgtggctcga gaaggcggta tcgggcgcat ggccgctcgg tgacgtgatc cggccgtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BupsA.17630.a.A1.PW31373
PURIFICATION DATe: 4/22/2011
CONCENTRATION: 24.93mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 33
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMADLPNHDA TLTETCIESE SVYDGAFLKV KRDTVRLPDG KHATREYVTH PGAVMVIPLF DNGRVLMESQ YRYPIGKVMA EFPAGKLDPN EGALACAVRE LREETGYTAR EYVFLTRIHP IISYSTEFID IYLARGLTAG ERKLDDGEFL ETFTATLPDL LEWVRTGRIS DVKTIIGTMW LEKAVSGAWP LGDVIRP
Validated NT Sequence
gtgctgttta ttacggccgg atcacgtcac cgagcggcca tgcgcccgat accgccttct cgagccacat cgtgccgatg atcgtcttga cgtcgctgat ccggccggtg cgcacccact cgagcaggtc cggcagcgtc gccgtgaacg tctcgaggaa ctcgccgtcg tcgagcttgc gctcgcccgc cgtcaggccg cgcgcgagat agatatcgat gaattcggtc gaatacgaga tgatcggatg aatgcgcgtg aggaacacgt attcgcgcgc ggtgtagccg gtttcctcac gcagttcgcg caccgcgcag gcgagcgcgc cttcgttcgg atcgagcttg cccgccggaa actcggccat caccttgccg atcggatagc ggtactggct ttccatcagc acgcggccgt tgtcgaacag cgggatgacc atcaccgcgc ccggatgcgt cacgtattcg cgcgtcgcat gcttgccgtc cggcagacga acggtgtcgc gcttgacctt caggaacgcg ccgtcgtaga cggattcgct ttcgatgcaa gtttcggtga gggtggcgtc gtgattcggc aaatcggcca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMADLPNHDA TLTETCIESE SVYDGAFLKV KRDTVRLPDG KHATREYVTH PGAVMVIPLF DNGRVLMESQ YRYPIGKVMA EFPAGKLDPN EGALACAVRE LREETGYTAR EYVFLTRIHP IISYSTEFID IYLARGLTAG ERKLDDGEFL ETFTATLPDL LEWVRTGRIS DVKTIIGTMW LEKAVSGAWP LGDVIRP
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCGATTTG CCGAATCACG ACGCCACCCT CACCGAAACT TGCATCGAAA GCGAATCCGT CTACGACGGC GCGTTCCTGA AGGTCAAGCG CGACACCGTT CGTCTGCCGG ACGGCAAGCA TGCGACGCGC GAATACGTGA CGCATCCGGG CGCGGTGATG GTCATCCCGC TGTTCGACAA CGGCCGCGTG CTGATGGAAA GCCAGTACCG CTATCCGATC GGCAAGGTGA TGGCCGAGTT TCCGGCGGGC AAGCTCGATC CGAACGAAGG CGCGCTCGCC TGCGCGGTGC GCGAACTGCG TGAGGAAACC GGCTACACCG CGCGCGAATA CGTGTTCCTC ACGCGCATTC ATCCGATCAT CTCGTATTCG ACCGAATTCA TCGATATCTA TCTCGCGCGC GGCCTGACGG CGGGCGAGCG CAAGCTCGAC GACGGCGAGT TCCTCGAGAC GTTCACGGCG ACGCTGCCGG ACCTGCTCGA GTGGGTGCGC ACCGGCCGGA TCAGCGACGT CAAGACGATC ATCGGCACGA TGTGGCTCGA GAAGGCGGTA TCGGGCGCAT GGCCGCTCGG TGACGTGATC CGGCCGTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA